Glutamine synthetase antibody

Principal name

Glutamine synthetase antibody

Alternative names for Glutamine synthetase antibody

GS, GLUL, GLNS, Glutamate decarboxylase, Glutamate-ammonia ligase

SwissProt ID

P04773 (Crigr), P09606 (Rat), P0A9C5 (Ecoli), P15103 (Bovin), P15104 (Human), P15105 (Mouse), P16580 (Chick), P46410 (Pig), P51121 (Xenla), P52782 (Luplu), Q6TEP3, Q8HZM5 (Canfa)

Gene ID

948370 (glnA), 2752 (GLUL), 4030465 (cysS)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Bac (Bacteria), Arabidopsis, Pl (Plant), Ze (Zebrafish), Can (Canine), Mky (Monkey), Por (Porcine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Goat, Mouse, Rabbit, Chicken

Available applications

Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Paraffin Sections (P), Flow Cytometry (F), Frozen Sections (C), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), EPair for Elisa (EPair)

Background of Glutamine synthetase antibody

Glutamine Synthetase catalyzes the conversion of ammonia and glutamate to glutamine. This reaction consumes a molecule of ATP:Glutamate + NH4+ + ATP , and produces, Glutamine + ADP + Pi. It is found in astrocytes as an octamer of identical 45kDa subunits. The function of Glutamine Synthetase is the detoxification of brain ammonia. It also has an important role in controlling metabolic regulations of neurotransmitter glutamate. Because of the multiple functions and importance of Glutamine Synthetase in cellular metabolism, both catalytic activities and synthesis are highly regulated. The activity of Glutamine Synthetase is controlled by adenylylation. Its activity is decreased in the cerebral cortex of brains affected by Alzheimer's disease, particularly in the vicinity of senile plaques. It is also decreased under conditions of glucose deprivation. On the other hands, the level of expression of Glutamine Synthetase is increased during ischemia in vivo or hypoxia in culture.

General readings

Vermeulen T., et al, (2008) Arch Biochem Biophys. 478:96-102.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Glutamine synthetase antibody

Immunohistochemical staining of paraffin-embedded Human breast tissue within the normal limits using anti-GLUL mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500700) Mouse IgG2a OTI1F4 Purified Hu F, ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Glutamine synthetase antibody

Western Blot analysis of GLUL expression in transfected 293T cell line by GLUL monoclonal antibody (M02), clone 3B6.

Lane 1: GLUL transfected lysate(42.1 KDa).
Lane 2: Non-transfected lysate. Mouse IgG1 3B6 Purified Hu, Ms, Rt E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Glutamine synthetase antibody

Western blot analysis of glutamine synthase. 40ug of lysates from mouse (lane M), rat (lane R), pig (lane P), bovine (lane B), or human (lane Hu) retina were probed. A 34 kDa band was identified in lysates from retinas of all species. Rabbit IgG Purified Bov, Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Glutamine synthetase antibody

Predicted band size: 42 kDa . Positive control: Rat and Mouse brain tissue and C6 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1,2,3: Rat and Mouse brain tissue and C6 cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Glutamine synthetase antibody

Western blot analysis of extracts of various cell lines, using GLUL antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

Glutamine synthetase antibody

WB Suggested Anti-GLUL Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Human brain Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Glutamine synthetase (N-term) antibody

Western blot analysis of GLUL Antibody (N-term) (AP17428PU-N) in mouse cerebellum tissue lysates (35 µg/lane). GLUL (arrow) was detected using the purified Pab. Rabbit Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Glutamine synthetase antibody

Glutamine synthetase Goat Serum E, IP, WB
2 ml / €1,150.00
  Acris Antibodies GmbH

Glutamine synthetase antibody

Glutamine synthetase Mouse Serum Hu E
50 µl / €290.00
  Abnova Taiwan Corp.

Glutamine synthetase antibody

GLUL MaxPab rabbit polyclonal antibody. Western Blot analysis of GLUL expression in mouse liver. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Glutamine synthetase antibody

Immunoperoxidase of monoclonal antibody to GLUL on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 0.5 ug/ml] Mouse IgG2a 2B12 Purified Hu E, P, WB
50 µg / €370.00
  Abnova Taiwan Corp.

Glutamine synthetase antibody

GLUL monoclonal antibody (M01), clone 2B12 Western Blot analysis of GLUL expression in HeLa ( Cat # L013V1 ). Mouse IgG2a 2B12 Ascites Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

Glutamine synthetase antibody

Immunoprecipitation analysis of cell lysate. Lane 1 : Input and lane 2 : Precipitates. Immunoblotting with GLUL monoclonal antibody, clone 8G9 ( Cat # MAB0734 ) . Mouse IgG2a 8G9 Hu IP
0.1 ml / €390.00
  Abnova Taiwan Corp.

Glutamine synthetase antibody

Immunoprecipitation analysis of cell lysate. Lane 1 : Input and lane 2 : Precipitates. Immunoblotting with GLUL monoclonal antibody, clone 27D10 ( Cat # MAB0735 ) . Mouse IgG2b 27D10 Hu C, IP
0.1 ml / €390.00
  Abnova Taiwan Corp.

Glutamine synthetase antibody

Immunoblot analysis of Lane 1 : 293T cell lysates transfected with Myc-Glutamine synthetase. Lane 2 : mouse brain, Lane 3 : mouse liver and Lane 4 : rat brain. Rabbit Hu WB
0.1 ml / €390.00
  Abnova Taiwan Corp.

Glutamine synthetase antibody

Localization of glutamine synthase in the retina.
Paraffin sections of mouse ( A, B ), rat ( C, D, G-I ) were reacted with GLUL polyclonal antibody ( Cat # PAB12567).
Staining red fluorescence in B, D, G, I, and brown immuno-peroxidase reaction.
Nuclei in some immunofluorescence experiments ( A-D ) were stained with DAPI ( shown in cyan ).
On inspection at low magnification, GLUL polyclonal antibody ( Cat # PAB12567) reacted with a single population of cells extending from the ganglion cell layer ( GCL ) through the inner nuclear layer ( INL ).
No signal was detected in controls either pre-incubated with 100 µg/ml of the immunizing peptide ( A ) or with pre-immune serum ( C ).
The pattern of staining observed in all experiments is typical of Muller cells.
This finding was confirmed by co-localization ( indicated by yellow in I) of GLUL ( red in G ) with antoher marker of GLUL ( green in H ). Rabbit IgG Bov, Hu, Ms, Rt ICC/IF, P
0.1 ml / €470.00
  Abnova Taiwan Corp.

Glutamine synthetase antibody

Glutamine synthetase Chicken Purified Bac WB
50 µl / €300.00
  AgriSera AB

Glutamine synthetase (pan plant) antibody

Glutamine synthetase Rabbit Serum Arabidopsis, Arabidopsis thaliana, Eragrostis tef, moss: Physcomitrella patens, Pl, red alga Gracilaria gracilis, Spinacia oleracea, Zea mays WB
50 µl / €390.00
  AgriSera AB

Glutamine synthetase (C-term) antibody

Glutamine synthetase Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

Glutamine synthetase antibody

Western blot analysis of Glutamine Synthetase expression in Jurkat (A); HeLa (B); HT29 (C) whole cell lysates. Rabbit Aff - Purified Bov, Can, Hu, Mky, Ms, Por, Rt, Ze WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

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