Glutathione reductase antibody
Principal name
Glutathione reductase antibody
Alternative names for Glutathione reductase antibody
GRase, GR, GSR, GLUR, GRD1
Ncbi ID
9606, NP_000628, NP_015234, NP_000628.2, NM_001195103, NP_034474.4, NM_001195104, NM_001195102
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Frozen Sections (C), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Dot blot (Dot), Immunodiffusion (ID), Radioimmunoassay (R), Flow Cytometry (F)
Background of Glutathione reductase antibody
- Kit Component:
- - KN307386G1, Gsr gRNA vector 1 in pCas-Guide vector
- KN307386G2, Gsr gRNA vector 2 in pCas-Guide vector
- KN307386D, donor vector containing left and right homologous arms and Luciferase-Puro functional cassette
- GE100003, scramble sequence in pCas-Guide vector - CRISPR Manual:
- CRISPR/CAS9 Genome Editing Manual
General readings
Satoh, N., et al. Biochem. Genet. 48 (9-10), 816-821 (2010)
Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010)
Wang, Y., et al. J. Hum. Genet. 55(8):490-494(2010)
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010)
Moyer, A.M., et al. Cancer Epidemiol. Biomarkers Prev. 19(3):811-821(2010)
Immunoperoxidase of monoclonal antibody to GSR on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 6 ug/ml]
Western blot analysis of GSR over-expressed 293 cell line, cotransfected with GSR Validated Chimera RNAi ( Cat # H00002936-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with GSR monoclonal antibody (M01), clone 6B4 (Cat # H00002936-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
GSR monoclonal antibody (M01), clone 6B4 Western Blot analysis of GSR expression in IMR-32 ( Cat # L008V1 ).
Western Blot analysis of GSR expression in transfected 293T cell line by GSR monoclonal antibody (M01), clone 6B4.Lane 1: GSR transfected lysate(56.3 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged GSR is approximately 0.03ng/ml as a capture antibody.
Immunoblot analysis of cell and tissue lysates. Lane 1 : HeLa cell lysate. Lane 2 : Jurkat cell lysate. Lane 3 : Mouse lung. Lane 4 : Rat lung.
Immunoprecipitation analysis of Hela cell lysates. Lane 1 : Input and lane 2 : Precipitates.
Immunoprecipitation analysis of Hela cell lysates. Lane 1 : Input and lane 2 : Precipitates. Immunoblotting with GSR monoclonal antibody, clone 1A7 ( Cat # MAB0693 ).
GSR MaxPab polyclonal antibody. Western Blot analysis of GSR expression in human placenta.
Western Blot analysis of GSR expression in transfected 293T cell line (H00002936-T01) by GSR MaxPab polyclonal antibody.Lane 1: GSR transfected lysate(57.42 KDa).Lane 2: Non-transfected lysate.
GSR MaxPab rabbit polyclonal antibody. Western Blot analysis of GSR expression in human colon.
Western Blot analysis of GSR expression in transfected 293T cell line (H00002936-T01) by GSR MaxPab polyclonal antibody.Lane 1: GSR transfected lysate(56.30 KDa).Lane 2: Non-transfected lysate.
Human Bronchial Epithelial Tissue; Rabbit Anti-GSR Antibody . Catalog Number: ARP54344_P050 . Formalin Fixed Paraffin Embedded Tissue: Human Bronchial Epithelial Tissue . Observed Staining: Cytoplasmic . Primary Antibody Concentration: 1:100 . Secondary Antibody: Donkey anti-Rabbit-Cy3 . Secondary Antibody Concentration: 1:200 . Magnification: 20X . Exposure Time: 0.5 - 2.0 sec .; Rabbit Anti-GSR Antibody (ARP54344_P050) ) in Human Bronchial Epithelial Tissue using Immunohistochemistry
Hum. Fetal Liver; Host: Rabbit. Target Name: FAM46C . Sample Tissue: Human Fetal Liver . Antibody Dilution: 1.0ug/ml.; GSR antibody - N-terminal region (ARP54344_P050) in Hum. Fetal Liver cells using Western Blot
Hum. Fetal Lung; Host: Rabbit. Target Name: NSUN6 . Sample Tissue: Human Fetal Lung . Antibody Dilution: 1.0ug/ml.; GSR antibody - N-terminal region (ARP54344_P050) in Hum. Fetal Lung cells using Western Blot
Hum. Adult Placenta; Host: Rabbit. Target Name: CHAD . Sample Tissue: Human Adult Placenta . Antibody Dilution: 1.0ug/ml.; GSR antibody - N-terminal region (ARP54344_P050) in Hum. Adult Placenta cells using Western Blot
Human HepG2; Host: Rabbit. Target Name: EGFL8 . Sample Tissue: HepG2 . Antibody Dilution: 1.0ug/ml. GSR is supported by BioGPS gene expression data to be expressed in HepG2.; GSR antibody - N-terminal region (ARP54344_P050) in Human HepG2 cells using Western Blot
Human 721_B; Host: Rabbit. Target Name: WT1 . Sample Tissue: 721_B . Antibody Dilution: 1.0ug/ml. GSR is supported by BioGPS gene expression data to be expressed in 721_B.; GSR antibody - N-terminal region (ARP54344_P050) in Human 721_B cells using Western Blot
zebrafish head; WB Suggested Anti-GSR Antibody . . Positive Control: Lane 1: 10ug untreated zebrafish head lysate Lane 2: 10ug 3hr H2O2 treated zebrafish head lysate Lane 3: 10ug 6hr H2O2 treated zebrafish head lysate . Primary Antibody Dilution : 1:1000. Secondary Antibody : Anti rabbit-HRP . Secondry Antibody Dilution : 1:10,000. Submitted by: Dr. Yuk Fai Leung, Purdue University; GSR antibody - N-terminal region (ARP54344_P050) in zebrafish head cells using Western Blot
Human HeLa; WB Suggested Anti-GSR Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Hela cell lysate; GSR antibody - N-terminal region (ARP54344_P050) in Human HeLa cells using Western Blot
Mouse heart; . Lanes: 1: 40ug mouse heart lysate, 2: 40ug mouse heart lysate. Primary Antibody Dilution: 1:1000. Secondary Antibody: Anti-rabbit HRP. Secondary Antibody Dilution: 1:10000. Gene Name: GSR. Submitted by: Anonymous.; GSR antibody - N-terminal region (ARP54344_P050) in Mouse heart cells using Western Blot
Immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma reacted with Glutathione reductase Antibody (C-term) Cat.-No AP51963PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining.
Confocal immunofluorescent analysis of Glutathione reductase Antibody (C-term) Cat.-No AP51963PU-N with NCI-H460 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Western blot analysis of Glutathione reductase Antibody (C-term) Cat.-No AP51963PU-N in NCI-H460 cell line lysates (35ug/lane).
Flow cytometric analysis of NCI-H460 cells using Glutathione reductase Antibody (C-term) Cat.-No AP51963PU-N (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
The cell lysates of HeLa, Jurkat and 293T(40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GSR antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.
Predicted band size: 56 kDa . Positive control: Jurkat, Hela and A549 cell lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lane1: Jurkat cell lysate Lane2: Hela cell lysate Lane3: A549 cell lysate Lysates: 40 ug per lane Primary antibody: 1/750 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds)
Predicted cell location: Cytoplasm . Positive control: Human ovarian cancer tissue . Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human ovarian cancer tissue using GSR antibody at dilution 1/50, on the right is treated with the fusion protein. (Original magnification:x200)
Predicted band size: 56 kDa . Positive control: Jurkat, Hela and A549 cell lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lane1: Jurkat cell lysate Lane2: Hela cell lysate Lane3: A549 cell lysate Lysates: 40 ug per lane Primary antibody: 1/750 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 3 seconds)
Predicted cell location: Cytoplasm . Positive control: Human cervical cancer tissue. Recommended dilution: 1/50-200 The image on the left is immunohistochemistry of paraffin-embedded human cervical cancer tissue using GSR antibody at dilution 1/50, on the right is treated with the fusion protein. (Original magnification:x200)
WB Suggested Anti-GSR Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: Hela cell lysate
Lanes: 1: 40ug mouse heart lysate, 2: 40ug mouse heart lysate; Primary Antibody Dilution: 1: 1000; Secondary Antibody: Anti-rabbit HRP; Secondary Antibody Dilution: 1: 10000; Gene Name: GSR; Submitted by: Anonymous;
WB Suggested Anti-GSR Antibody; Positive Control: Lane 1: 10 ug untreated zebrafish head lysate Lane 2: 10 ug 3hr H2O2 treated zebrafish head lysate Lane 3: 10ug 6hr H2O2 treated zebrafish head lysate; Primary Antibody Dilution: 1: 1000; Secondary Antibody: Anti rabbit-HRP; Secondry Antibody Dilution: 1: 10, 000; Submitted by: Dr. Yuk Fai Leung, Purdue University
6Hum. Adult Placenta; Host: Rabbit; Target Name: CHAD; Sample Tissue: Human Adult Placenta; Antibody Dilution: 1.0 ug/ml
7Human HepG2; Host: Rabbit; Target Name: EGFL8; Sample Tissue: HepG2; Antibody Dilution: 1.0 ug/ml GSR is supported by BioGPS gene expression data to be expressed in HepG2
5Hum. Fetal Liver; Host: Rabbit; Target Name: FAM46C; Sample Tissue: Human Fetal Liver; Antibody Dilution: 1.0 ug/ml
4Hum. Fetal Lung; Host: Rabbit; Target Name: NSUN6; Sample Tissue: Human Fetal Lung; Antibody Dilution: 1.0 ug/ml
8Human 721_B; Host: Rabbit; Target Name: WT1; Sample Tissue: 721_B; Antibody Dilution: 1.0 ug/ml. GSR is supported by BioGPS gene expression data to be expressed in 721_B
Rabbit Anti-GSR Antibody; Formalin Fixed Paraffin Embedded Tissue: Human Bronchial Epithelial Tissue; Observed Staining: Cytoplasmic; Primary Antibody Concentration: 1: 100; Secondary Antibody: Donkey anti-Rabbit-Cy3; Secondary Antibody Concentration: 1: 200; Magnification: 20X; Exposure Time: 0.5 - 2.0 sec;
- Quick-Filters:
- Highly Validated (2)
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA336225 | Glutathione reductase antibody |
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| Rabbit Polyclonal Anti-GSR Antibody | |||||||
 |
Rabbit | IgG | Purified | Bov, GP, Hu, Ms, Por, Rb, Rt | ICC/IF, WB |
50 µg /
€360.00
|
|
| OriGene Technologies, Inc. | |||||||
 +8 additional images |
Highly validated antibody! |
||||||
| AP51963PU-N | Glutathione reductase (C-term) antibody |
||||||
 |
Rabbit | Ig | Aff - Purified | Hu | F, ICC/IF, P, WB |
0.4 ml /
€370.00
|
|
| Acris Antibodies GmbH | |||||||
| +3 additional images |
Highly validated antibody! |
||||||
| TA323576 | Glutathione reductase antibody |
||||||
| Anti-GSR Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| TA323577 | Glutathione reductase antibody |
||||||
| Anti-GSR Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P, WB |
0.1 ml /
€335.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
|||||||
| AM50347PU-N | Glutathione reductase antibody |
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 |
Mouse | IgG1 | AT11D10 | Purified | Hu | E, WB |
0.1 ml /
€310.00
|
| Acris Antibodies GmbH | |||||||
| AM50347PU-S | Glutathione reductase antibody |
||||||
|
Mouse | IgG1 | AT11D10 | Purified | Hu | E, WB |
50 µl /
€220.00
|
| Acris Antibodies GmbH | |||||||
| AP21307AF-N | Glutathione reductase antibody |
||||||
 |
Rabbit | IgG | Azide Free | Bakers Yeast | Dot, E, ICC/IF, ID, IP, R, WB |
10 mg /
€240.00
|
|
| Acris Antibodies GmbH | |||||||
| AP21307BT-N | Glutathione reductase antibody |
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|
Rabbit | IgG | Biotin | Bakers Yeast | Dot, E, ICC/IF, ID, IP, R, WB |
1 ml /
€360.00
|
|
| Acris Antibodies GmbH | |||||||
| AP21307PU-N | Glutathione reductase antibody |
||||||
|
Rabbit | IgG | Aff - Purified | Bakers Yeast | Dot, E, ICC/IF, ID, IP, R, WB |
1 mg /
€450.00
|
|
| Acris Antibodies GmbH | |||||||
CRISPR
| Catalog No. | Species | Donor Vector | |
|---|---|---|---|
| KN209652 | GSR - human gene knockout kit via CRISPR |
||
|
Human | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN209652BN | GSR - human gene knockout kit via CRISPR |
||
|
Human | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN209652LP | GSR - human gene knockout kit via CRISPR |
||
|
Human | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN209652RB | GSR - human gene knockout kit via CRISPR |
||
|
Human | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN307386 | Gsr - mouse gene knockout kit via CRISPR |
||
|
Mouse | GFP-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN307386BN | Gsr - mouse gene knockout kit via CRISPR |
||
|
Mouse | mBFP-Neo |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN307386LP | Gsr - mouse gene knockout kit via CRISPR |
||
|
Mouse | Luciferase-Puro |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
| KN307386RB | Gsr - mouse gene knockout kit via CRISPR |
||
|
Mouse | RFP-BSD |
1 kit /
€1,290.00
|
| OriGene Technologies, Inc. | |||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP309652 | Glutathione reductase |
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| Recombinant protein of human glutathione reductase (GSR) | |||||
 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€680.00
|
|
| OriGene Technologies, Inc. | |||||
| AR50143PU-N | Glutathione reductase (43-522, His-tag) |
||||
 |
Human | Purified | > 95 % by SDS - PAGE | E. coli |
0.25 mg /
€730.00
|
| Acris Antibodies GmbH | |||||
| AR50143PU-S | Glutathione reductase (43-522, His-tag) |
||||
|
Human | Purified | > 95 % by SDS - PAGE | E. coli |
50 µg /
€300.00
|
| Acris Antibodies GmbH | |||||
