Glycogen Synthase antibody

Principal name

Glycogen Synthase antibody

Alternative names for Glycogen Synthase antibody

GYS1, GYS, muscle Glycogen Synthase

SwissProt ID

A2RRU1 (Rat), A7MB78 (Bovin), P13807 (Human), P13834 (Rabit), Q9Z1E4 (Mouse)

Gene ID

2997 (GYS1), 29974 (A1CF)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Flow Cytometry (F), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Frozen Sections (C)

Background of Glycogen Synthase antibody

Glycogen (starch) synthase belongs to the mammalian/fungal glycogen synthase family of proteins. Two forms of this protein exist, a liver form and a muscle form, both of which have the same function in the glycogen biosynthesis pathway. Glycogen synthase transfers the glycosyl residue from UDP-glucose to the nonreducing end of α-1,4-glucan. The 81 kDa liver glycogen synthase protein is truncated by 34 amino acids compared to the muscle form. However, these enzymes differ significantly in their amino- and carboxyl-terminal regions. Muscle glycogen synthase serves to fuel muscular activity only and is regulated by muscle contraction and by catecholamines. Liver glycogen synthase mediates blood glucose homeostasis in response to nutritional cues. Defects in the gene encoding liver glycogen synthase results in glycogen storage disease type 0 (GSD0), a rare form of fasting ketotic hypoglycemia.

General readings

DiGiovanna MP et al. Production of Polyclonal anti-Phosphopeptide Antibodies. Curr. Prot. Immunol. 11:6.1-6.19 (1996).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Glycogen Synthase antibody

Immunohistochemistry (IHC) analysis of GYS antibody (Cat.-No AP06146PU-N) on paraffin-embedded sections. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Glycogen Synthase antibody

Predicted band size: 84 kDa . Positive control: Hela and 293 cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1,2: Hela and 293 cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Glycogen Synthase antibody

Western blot analysis of Glycogen Synthase expression in Serum treated HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Glycogen Synthase antibody

Glycogen Synthase Mouse IgG1 3A7 Ascites Hu E, WB
0.1 ml / €430.00
  Acris Antibodies GmbH

Glycogen Synthase pSer641 antibody

Figure 1. Formalin-Fixed Paraffin-Embedded (FFPE) on Prostate. Rabbit Aff - Purified Hu, Ms E, IP, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Glycogen Synthase pSer645 antibody

Western blot analysis of extracts from NIH-3T3 cells treated with PMA using Phospho-Glycogen Synthase (Ser645) antibody AP55897PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 ml / €340.00
  Acris Antibodies GmbH

Glycogen Synthase pSer645 antibody

Western blot analysis of extracts from NIH-3T3 cells treated with PMA using Phospho-Glycogen Synthase (Ser645) antibody AP55897PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µl / €270.00
  Acris Antibodies GmbH

Glycogen Synthase pSer641 antibody

Figure 1. Immunoblotting. Affinity Purified Phosphospecific antibody to human muscle Glycogen Synthase (GS) at pS640 was used at a 1:1000 dilution to detect human muscle GS by Western blot. Approximately 12 ul of a mouse cardiac myocyte lysate was loaded per lane on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock treated (lanes 1 and 5) or insulin treated at 10 nM, 100 nM and 1 µM (lanes 2, 3 and 4 respectively) for 15' or CLA treated at 4nM, 20 nM or 100 nM (lanes 6,7 and 8 respectively) for 45'. After washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG preceded color development using Amersham's substrate system. Other detection methods will yield similar results. Rabbit Aff - Purified Hu, Ms E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

Glycogen Synthase pSer641 antibody

Glycogen Synthase Rabbit Hu, Ms C, E, WB
0.1 mg / €560.00
  Abnova Taiwan Corp.

Glycogen Synthase pSer645 antibody

Immunohistochemistry analysis of paraffin-embedded human skeletal muscle, using Glycogen Synthase (Phospho-Ser645) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €205.00
  Assay Biotech

Glycogen Synthase pSer645 antibody

Immunohistochemistry analysis of paraffin-embedded human skeletal muscle, using Glycogen Synthase (Phospho-Ser645) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €310.00
  Assay Biotech

Glycogen Synthase antibody

Western blot analysis of extracts from HeLa cells, treated with Serum 20% 30', using Glycogen Synthase (Ab-645) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

Glycogen Synthase antibody

Western blot analysis of extracts from HeLa cells, treated with Serum 20% 30', using Glycogen Synthase (Ab-645) Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

Glycogen Synthase antibody

Western blot analysis of GYS1 expression in SW626 (A); SKOV3 (B); HeLa (C); K562 (D); HepG2 (E); mouse liver (F) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Por, Rb, Rt WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

Glycogen Synthase antibody

Western blot analysis of GYS1 expression in SW626 (A); SKOV3 (B); HeLa (C); K562 (D); HepG2 (E); mouse liver (F) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Por, Rb, Rt WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

Glycogen Synthase antibody

Western blot analysis of GYS1 expression in SW626 (A); SKOV3 (B); HeLa (C); K562 (D); HepG2 (E); mouse liver (F) whole cell lysates. Rabbit Aff - Purified Bov, Hu, Ms, Por, Rb, Rt WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

Glycogen Synthase antibody

Immunohistochemistry: Glycogen Synthase Antibody (EP816Y) [NB110-57009] - Immunohistochemical staining of paraffin-embedded human muscle using anti-Glycogen Synthase (cat. # 110-57009). Rabbit IgG EP816Y Aff - Purified Hu, Ms, Rt ICC/IF, IP, P, WB
0.1 ml / €480.00
  Novus Biologicals Inc.

Glycogen Synthase (C-term) antibody

Immunofluorescent: Glycogen Synthase (C-term) Antibody (EP817Y) [NB110-57010] - Immunofluorescent staining of HeLa cells using anti-Glycogen Synthase (C-term) (cat. # 110-57010). Rabbit IgG EP817Y Aff - Purified Hu, Ms, Rt F, ICC/IF, IP, P, WB
0.1 ml / €480.00
  Novus Biologicals Inc.

Glycogen Synthase pSer641 antibody

Western Blot: Glycogen Synthase Phosphospecific Antibody (EP852Y) [NB110-57011] - Western blot analysis on mouse muscle lysates using anti-Phospho-Glycogen Synthase (pS641) (NB110-57011), 1:10,000 dilution. Cells were either (A) untreated (B) treated with AP. Rabbit IgG EP852Y Aff - Purified Hu, Ms ICC/IF, IP, P, WB
0.1 ml / €480.00
  Novus Biologicals Inc.

Glycogen Synthase pSer641 antibody

Western Blot: Glycogen Synthase [p Ser640] Antibody [NB600-611] - Immunoblotting. Affinity Purified Phosphospecific antibody to human muscle Glycogen Synthase (GS) at pS640 was used at a 1:1000 dilution to detect human muscle GS by Western blot. Approximately 12 ul of a mouse cardiac myocyte lysate was loaded per lane on a 4-20% Criterion gel for SDS-PAGE. Samples were either mock treated (lanes 1 and 5) or insulin treated at 10 nM, 100 nM and 1 uM (lanes 2, 3 and 4 respectively) for 15 min or CLA treated at 4nM, 20 nM or 100 nM (lanes 6,7 and 8 respectively) for 45 min. After washing, a 1:5,000 dilution of HRP conjugated Gt-a-Rabbit IgG preceded color development using Amersham's substrate system. Other detection methods will yield similar results. Rabbit Aff - Purified Hu E, P, WB
50 µg / €320.00
  Novus Biologicals Inc.

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