Glycyl-tRNA synthetase antibody

Principal name

Glycyl-tRNA synthetase antibody

Alternative names for Glycyl-tRNA synthetase antibody

GARS, Glycine-tRNA ligase, GlyRS, AP-4-A synthetase, SMAD1, Diadenosine tetraphosphate synthetase

SwissProt ID

P41250 (Human), Q5I0G4 (Rat), Q9CZD3 (Mouse)

Gene ID

2617 (GARS)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), Ze (Zebrafish), African clawed frog, Can (Canine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), Western blot / Immunoblot (WB), ELISA (detection) (E(detection))

Background of Glycyl-tRNA synthetase antibody

GARS catalyzes the attachment of glycine to tRNA(Gly). Is also able produce diadenosine tetraphosphate (Ap4A), a universal pleiotropic signaling molecule needed for cell regulation pathways, by direct condensation of 2 ATPs.This gene encodes glycyl-tRNA synthetase, one of the aminoacyl-tRNA synthetases that charge tRNAs with their cognate amino acids. The encoded enzyme is an (alpha)2 dimer which belongs to the class II family of tRNA synthetases. It has been shown to be a target of autoantibodies in the human autoimmune diseases, polymyositis or dermatomyositis. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Guo R.-T., et al. (2009) J. Biol. Chem. 284:28968-28976Xie W., et al. (2007) Proc. Natl. Acad. Sci. U.S.A. 104:9976-9981

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Glycyl-tRNA synthetase antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human colon tissue using anti-GARS mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805168) Mouse IgG2b OTI6C5 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Glycyl-tRNA synthetase antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-GARS mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805281) Mouse IgG1 OTI4G4 Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Glycyl-tRNA synthetase antibody

Western blot analysis of extracts of various cell lines, using GARS antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

Glycyl-tRNA synthetase antibody

WB Suggested Anti-GARS Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: MCF7 cell lysate Rabbit IgG Purified Bov, Can, GP, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

Glycyl-tRNA synthetase antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-GARS mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA805076) Mouse IgG2b OTI4H8 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Glycyl-tRNA synthetase antibody

The cell lysates of Hela and 293T (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GARS antibody 1:500. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 AT4E10 Purified Hu E, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

Glycyl-tRNA synthetase antibody

The cell lysates of Hela and 293T (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GARS antibody 1:500. Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Mouse IgG1 AT4E10 Purified Hu E, WB
50 µl / €220.00
  Acris Antibodies GmbH

Glycyl-tRNA synthetase (C-term) antibody

Western blot analysis of anti-GARS Antibody (C-term) in Ramos cell line lysates (35ug/lane). GARS (arrow) was detected using the purified Pab. Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Glycyl-tRNA synthetase antibody

GARS MaxPab polyclonal antibody. Western Blot analysis of GARS expression in human placenta. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Glycyl-tRNA synthetase antibody

Glycyl-tRNA synthetase Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

Glycyl-tRNA synthetase antibody

Western Blot analysis of GARS expression in transfected 293T cell line (H00002617-T01) by GARS MaxPab polyclonal antibody.

Lane 1: GARS transfected lysate(77.50 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Glycyl-tRNA synthetase antibody

Human MCF-7; WB Suggested Anti-GARS Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: MCF7 cell lysate; GARS antibody - middle region (ARP58758_P050) in Human MCF-7 cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

Glycyl-tRNA synthetase antibody

Western Blot: GARS Antibody [NBP1-54602] - This Anti-GARS antibody was used in Western Blot of MCF7     tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €440.00
  Novus Biologicals Inc.

Glycyl-tRNA synthetase antibody

Immunohistochemistry-Paraffin: GARS Antibody [NBP1-85533] - Immunohistochemical staining of human cerebral cortex shows strong cytoplasmic positivity in neuronal cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Glycyl-tRNA synthetase antibody

Immunohistochemistry-Paraffin: GARS Antibody [NBP1-85534] - Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Glycyl-tRNA synthetase

Glycyl-tRNA synthetase Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

Glycyl-tRNA synthetase (43-289, His-tag)

Glycyl-tRNA synthetase Human Purified > 85 % by SDS - PAGE E. coli
0.25 mg / €940.00
  Acris Antibodies GmbH

Glycyl-tRNA synthetase (43-289, His-tag)

Glycyl-tRNA synthetase Human Purified > 85 % by SDS - PAGE E. coli
50 µg / €370.00
  Acris Antibodies GmbH

Glycyl-tRNA synthetase

Glycyl-tRNA synthetase Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

Glycyl-tRNA synthetase

Glycyl-tRNA synthetase Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Items 1 to 20 of 23 total

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