Glypican-1 / GPC1 antibody

Principal name

Glypican-1 / GPC1 antibody

SwissProt ID

P35052 (Human), P35053 (Rat), P50593 (Chick), Q9QZF2 (Mouse)

Gene ID

2817 (GPC1)

Available reactivities

Hu (Human), Ms (Mouse), Can (Canine), Macaque, Op (Opossum), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F), Frozen Sections (C)

Background of Glypican-1 / GPC1 antibody

Glypican-1 (also known as GPC1 or FLJ38078) is a member of the glypican-related integral membrane proteoglycan family (GRIPS). This protein is a heparan sulfate proteoglycan which is composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains and are anchored to the cell surface via a covalent linkage to glycosylphosphatidlyinositol (GPI). Glypicans can modify cell signaling pathways and contribute to cellular proliferation and tissue growth. In humans, glypican-1 is over expressed in breast and brain cancers (gliomas). All glypicans contain an N-terminal signal peptide and a hydrophobic domain in their C-terminal region which is required for attachment of the GPI anchor. The amino acid sequences of the six vertebrate glypican family members vary
from 17% to 63% identity. The location of 14 cysteine amino acids is conserved between the glypicans, suggesting the existence of a highly similar three-dimensional structure. Heparan sulfate glycosaminoglycan chains are attached at the 50 amino acids at the C-terminal end of the protein, near the anchor and the cell membrane. Glypican functions as coreceptor for a variety of growth factors. Glypican-1 has been shown to interact with SLIT2.

General readings

Sjoeblom T., et.al., Science 314:268-274(2006)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Glypican-1 / GPC1 antibody

WB of Rabbit anti-Glypican-1 antibody. Lane 1: untransfected 293T cell lysate. Lane 2: 293T whole cell lysate. Load: 50ug per lane. Primary antibody: Glypican-1 antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP Gt-a-Rabbit IgG diluted 1:5,000 at 4° C. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 61kDa, 61kDa for Glypican-1. Other band(s): 110kDa is likely due to the presence of the Fc-tag. Minor bands may represent post translational modifications of glypican-1. Rabbit IgG Aff - Purified Can, Hu, Macaque, Ms, Op, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Glypican-1 / GPC1 antibody

A band of approximately 64kDa is detected in normal mouse brain extract.
Lane 1: AM31485AF-N 5 µg/ml
Lane 2: AM31485AF-N 1 µg/ml
Lane 3: Isotype control 5 µg/ml
Lane 4: Isotype control 1 µg/ml Mouse IgG1 B485M Azide Free Hu E, ICC/IF, WB
0.1 mg / €540.00
  Acris Antibodies GmbH

Glypican-1 / GPC1 antibody

WB of Rabbit anti-Glypican-1 antibody. Lane 1: untransfected 293T cell lysate. Lane 2: 293T whole cell lysate. Load: 50ug per lane. Primary antibody: Glypican-1 antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP Gt-a-Rabbit IgG diluted 1:5,000 at 4° C. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 61kDa, 61kDa for Glypican-1. Other band(s): 110kDa is likely due to the presence of the Fc-tag. Minor bands may represent post translational modifications of glypican-1. Rabbit IgG Aff - Purified Can, Hu, Macaque, Ms, Op, Rt E, WB
25 µl / €190.00
  Acris Antibodies GmbH

Glypican-1 / GPC1 (N-term) antibody

Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human lung carcinoma reacted with Glypican-1 / GPC1Antibody (N-term) Cat.-No AP51865PU-N, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Glypican-1 / GPC1 antibody

Anti-Glypican-1 antibody ICC staining of HeLa cells (green). Plasma membranes are labelled in red and nuclei in blue. Rabbit Serum Hu C, E, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

Glypican-1 / GPC1 antibody

Glypican-1 / GPC1 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Glypican-1 / GPC1 antibody

Glypican-1 / GPC1 Rabbit IgG Hu ICC/IF, P, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

Glypican-1 / GPC1 antibody

Western Blot: Glypican 1/ GPC1 Antibody [NBP1-42718] - protein A purified anti-Glypican-1 antibody shows detection of  exogenous glypican-1 in 50ug 293T whole cell lysate (lane 2).  Minor bands may represent post translational modifications of  glypican-1. The blot was incubated overnight with primary  antibody at a 1:1,000 dilution followed by washes and  exposure to the secondary antibody HRP Gt-a-Rabbit IgG  diluted 1:5,000 in 5% BLOTTO at 4° C. Lane 1:  untransfected 293T cell lysate. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €510.00
  Novus Biologicals Inc.

Glypican-1 / GPC1 antibody

Immunohistochemistry-Paraffin: Glypican 1/ GPC1 Antibody [NBP1-89759] - Immunohistochemical staining of human duodenum shows moderate positivity in luminal membrane. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Glypican-1 / GPC1

Glypican-1 / GPC1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

Glypican-1 / GPC1

Glypican-1 / GPC1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

GPC1 overexpression lysate

GPC1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

12 Item(s)

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