Hu (Human), Ms (Mouse), Can (Canine), Macaque, Op (Opossum), Rt (Rat)
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F), Frozen Sections (C)
Background of Glypican-1 / GPC1 antibody
Glypican-1 (also known as GPC1 or FLJ38078) is a member of the glypican-related integral membrane proteoglycan family (GRIPS). This protein is a heparan sulfate proteoglycan which is composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains and are anchored to the cell surface via a covalent linkage to glycosylphosphatidlyinositol (GPI). Glypicans can modify cell signaling pathways and contribute to cellular proliferation and tissue growth. In humans, glypican-1 is over expressed in breast and brain cancers (gliomas). All glypicans contain an N-terminal signal peptide and a hydrophobic domain in their C-terminal region which is required for attachment of the GPI anchor. The amino acid sequences of the six vertebrate glypican family members vary from 17% to 63% identity. The location of 14 cysteine amino acids is conserved between the glypicans, suggesting the existence of a highly similar three-dimensional structure. Heparan sulfate glycosaminoglycan chains are attached at the 50 amino acids at the C-terminal end of the protein, near the anchor and the cell membrane. Glypican functions as coreceptor for a variety of growth factors. Glypican-1 has been shown to interact with SLIT2.
Sjoeblom T., et.al., Science 314:268-274(2006)
Immunohistochemical analysis of paraffin-embedded DLD-1 xenograft, using Glypican-1(GTX104557) antibody at 1:100 dilution.
Western blot using Protein A purified anti-Glypican-1 antibody shows detection of exogenous Glypican-1 in 50 µg 293T whole cell lysate (Lane 2). Minor bands may represent post translational modifications of glypican-1. The blot was incubated overnight with primary antibody at a 1/1,000 dilution followed by washes and exposure to the secondary antibody HRP Goat anti-Rabbit IgG diluted 1/5,000 in 5% BLOTTO at 4°C. Lane 1: untransfected 293T cell lysate.Personal Communication, Caterina Bianco, CCR-NCI, Bethesda, MD.
Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human lung carcinoma reacted with Glypican-1 / GPC1Antibody (N-term) Cat.-No AP51865PU-N, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of Hela cells using Glypican-1 / GPC1Antibody (N-term) Cat.-No AP51865PU-N (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western Blot: Glypican 1/ GPC1 Antibody [NBP1-42718] - protein A purified anti-Glypican-1 antibody shows detection of exogenous glypican-1 in 50ug 293T whole cell lysate (lane 2). Minor bands may represent post translational modifications of glypican-1. The blot was incubated overnight with primary antibody at a 1:1,000 dilution followed by washes and exposure to the secondary antibody HRP Gt-a-Rabbit IgG diluted 1:5,000 in 5% BLOTTO at 4° C. Lane 1: untransfected 293T cell lysate.
A band of approximately 64kDa is 1 2 3 4 detected in normal mouse brain extract. x000DLane 1: GTX42664 5ug/ml x000DLane 2: GTX42664 1ug/ml x000DLane 3: Isotype control 5ug/ml x000DLane 4: Isotype control 1ug/ml
Formalin-fixed and paraffin embedded rat brain labeled with Anti-Glypican 1/GPC1 Polyclonal Antibody, Unconjugated (bs-2426R) at 1:200 followed by conjugation to the secondary antibody and DAB staining.