Glypican-1 / GPC1 antibody

Principal name

Glypican-1 / GPC1 antibody

SwissProt ID

P35052 (Human), P35053 (Rat), P50593 (Chick), Q9QZF2 (Mouse)

Gene ID

2817 (GPC1), 14733 (Gpc1), 58920

Available reactivities

Hu (Human), Can (Canine), Macaque, Ms (Mouse), Op (Opossum), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F), Frozen Sections (C)

Background of Glypican-1 / GPC1 antibody

Glypican-1 (also known as GPC1 or FLJ38078) is a member of the glypican-related integral membrane proteoglycan family (GRIPS). This protein is a heparan sulfate proteoglycan which is composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains and are anchored to the cell surface via a covalent linkage to glycosylphosphatidlyinositol (GPI). Glypicans can modify cell signaling pathways and contribute to cellular proliferation and tissue growth. In humans, glypican-1 is over expressed in breast and brain cancers (gliomas). All glypicans contain an N-terminal signal peptide and a hydrophobic domain in their C-terminal region which is required for attachment of the GPI anchor. The amino acid sequences of the six vertebrate glypican family members vary from 17% to 63% identity. The location of 14 cysteine amino acids is conserved between the glypicans, suggesting the existence of a highly similar three-dimensional structure. Heparan sulfate glycosaminoglycan chains are attached at the 50 amino acids at the C-terminal end of the protein, near the anchor and the cell membrane. Glypican functions as coreceptor for a variety of growth factors. Glypican-1 has been shown to interact with SLIT2.

General readings

Sjoeblom T., et.al., Science 314:268-274(2006)

15 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Glypican-1 / GPC1 antibody

  Rabbit polyclonal Glypican-1 antibody  
WB of Rabbit anti-Glypican-1 antibody. Lane 1: untransfected 293T cell lysate. Lane 2: 293T whole cell lysate. Load: 50ug per lane. Primary antibody: Glypican-1 antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP Gt-a-Rabbit IgG diluted 1:5,000 at 4° C. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 61kDa, 61kDa for Glypican-1. Other band(s): 110kDa is likely due to the presence of the Fc-tag. Minor bands may represent post translational modifications of glypican-1. Rabbit IgG Aff - Purified Can, Hu, Macaque, Ms, Op, Rt E, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

Glypican-1 / GPC1 antibody

A band of approximately 64kDa is detected in normal mouse brain extract.
Lane 1: AM31485AF-N 5 µg/ml
Lane 2: AM31485AF-N 1 µg/ml
Lane 3: Isotype control 5 µg/ml
Lane 4: Isotype control 1 µg/ml Mouse IgG1 B485M Azide Free Hu E, ICC/IF, WB
0.1 mg / €600.00
  Acris Antibodies GmbH

Glypican-1 / GPC1 (N-term) antibody

Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human lung carcinoma reacted with Glypican-1 / GPC1Antibody (N-term) Cat.-No AP51865PU-N, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Glypican-1 / GPC1 antibody

Anti-Glypican-1 antibody ICC staining of HeLa cells (green). Plasma membranes are labelled in red and nuclei in blue. Rabbit Serum Hu C, E, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

Gpc1 - mouse gene knockout kit via CRISPR

Gpc1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Gpc1 - mouse gene knockout kit via CRISPR

Gpc1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Gpc1 - mouse gene knockout kit via CRISPR

Gpc1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Gpc1 - mouse gene knockout kit via CRISPR

Gpc1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

GPC1 overexpression lysate

GPC1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, GPC1 (mGFP-tagged) - Human glypican 1 (GPC1), 200 uL, >10^7 TU/mL

Lenti ORF particles, GPC1 (mGFP-tagged) - Human glypican 1 (GPC1), 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, GPC1 (Myc-DDK tagged) - Human glypican 1 (GPC1) , 200 uL, >10^7 TU/mL

Lenti ORF particles, GPC1 (Myc-DDK tagged) - Human glypican 1 (GPC1) , 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Gpc1 (GFP-tagged) - Mouse glypican 1 (Gpc1), 200 uL, >10^7 TU/mL

Lenti ORF particles, Gpc1 (GFP-tagged) - Mouse glypican 1 (Gpc1), 200 uL, >10^7 TU/mL  
200 µl / €1,190.00
  OriGene Technologies, Inc.

Lenti ORF particles, Gpc1 (Myc-DDK-tagged) - Mouse glypican 1 (Gpc1), 200 uL, >10^7 TU/mL

Lenti ORF particles, Gpc1 (Myc-DDK-tagged) - Mouse glypican 1 (Gpc1), 200 uL, >10^7 TU/mL  
200 µl / €1,070.00
  OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Gpc1 (GFP-tagged ORF) - Rat glypican 1 (Gpc1), 200 uL, >10^7 TU/mL

Lenti ORF particles, Gpc1 (GFP-tagged ORF) - Rat glypican 1 (Gpc1), 200 uL, >10^7 TU/mL  
200 µl / €1,200.00
  OriGene Technologies, Inc.

Lenti ORF particles, Gpc1 (Myc-DDK-tagged ORF) - Rat glypican 1 (Gpc1), 200 uL, >10^7 TU/mL

Lenti ORF particles, Gpc1 (Myc-DDK-tagged ORF) - Rat glypican 1 (Gpc1), 200 uL, >10^7 TU/mL  
200 µl / €1,071.00
  OriGene Technologies, Inc.

15 Item(s)

per page
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