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Glypican-1 / GPC1 antibody

Principal name

Glypican-1 / GPC1 antibody

SwissProt ID

P35052 (Human), P35053 (Rat), P50593 (Chick), Q9QZF2 (Mouse)

Gene ID

2817 (GPC1), 14733 (Gpc1), 58920

Ncbi ID

9606, NP_002072.2, NP_002072, NM_002081, NP_057905.1, NM_016696, NM_030828

Available reactivities

Hu (Human), Can (Canine), Macaque, Ms (Mouse), Op (Opossum), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F), Frozen Sections (C)

Background of Glypican-1 / GPC1 antibody

Glypican-1 (also known as GPC1 or FLJ38078) is a member of the glypican-related integral membrane proteoglycan family (GRIPS). This protein is a heparan sulfate proteoglycan which is composed of a membrane-associated protein core substituted with a variable number of heparan sulfate chains and are anchored to the cell surface via a covalent linkage to glycosylphosphatidlyinositol (GPI). Glypicans can modify cell signaling pathways and contribute to cellular proliferation and tissue growth. In humans, glypican-1 is over expressed in breast and brain cancers (gliomas). All glypicans contain an N-terminal signal peptide and a hydrophobic domain in their C-terminal region which is required for attachment of the GPI anchor. The amino acid sequences of the six vertebrate glypican family members vary from 17% to 63% identity. The location of 14 cysteine amino acids is conserved between the glypicans, suggesting the existence of a highly similar three-dimensional structure. Heparan sulfate glycosaminoglycan chains are attached at the 50 amino acids at the C-terminal end of the protein, near the anchor and the cell membrane. Glypican functions as coreceptor for a variety of growth factors. Glypican-1 has been shown to interact with SLIT2.

General readings

Sjoeblom T., et.al., Science 314:268-274(2006)

WB of Rabbit anti-Glypican-1 antibody. Lane 1: untransfected 293T cell lysate. Lane 2: 293T whole cell lysate. Load: 50ug per lane. Primary antibody: Glypican-1 antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP Gt-a-Rabbit IgG diluted 1:5,000 at 4° C. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 61kDa, 61kDa for Glypican-1. Other band(s): 110kDa is likely due to the presence of the Fc-tag. Minor bands may represent post translational modifications of glypican-1.

A band of approximately 64kDa is detected in normal mouse brain extract.Lane 1: AM31485AF-N 5 µg/mlLane 2: AM31485AF-N 1 µg/mlLane 3: Isotype control 5 µg/mlLane 4: Isotype control 1 µg/ml

AM31485AF-N

Immunohistochemistry analysis in formalin-fixed and paraffin-embedded human lung carcinoma reacted with Glypican-1 / GPC1Antibody (N-term) Cat.-No AP51865PU-N, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

AP51865PU-N

Western blot analysis of Glypican-1 / GPC1Antibody (N-term) Cat.-No AP51865PU-N in Hela cell line lysates (35ug/lane). Glypican-1 (arrow) was detected using the purified Pab.

AP51865PU-N

Flow cytometric analysis of Hela cells using Glypican-1 / GPC1Antibody (N-term) Cat.-No AP51865PU-N (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

AP51865PU-N

Anti-Glypican-1 antibody ICC staining of HeLa cells (green). Plasma membranes are labelled in red and nuclei in blue.

AP54982SU-N

Primary Antibodies

Catalog No.	Host	Iso.	Clone	Pres.	React.	Applications
TA319424	Glypican-1 / GPC1 antibody
	Rabbit polyclonal Glypican-1 antibody
	Rabbit	IgG		Aff - Purified	Can, Hu, Macaque, Ms, Op, Rt	E, WB	0.1 mg / €356.00
							OriGene Technologies, Inc.
AM31485AF-N	Glypican-1 / GPC1 antibody
	Mouse	IgG1	B485M	Azide Free	Hu	E, ICC/IF, WB	0.1 mg / €600.00
							Acris Antibodies GmbH
AP51865PU-N	Glypican-1 / GPC1 (N-term) antibody
	Rabbit	Ig		Purified	Hu	F, P, WB	0.4 ml / €370.00
							Acris Antibodies GmbH
+2 additional images
AP54982SU-N	Glypican-1 / GPC1 antibody
	Rabbit			Serum	Hu	C, E, WB	0.2 ml / €400.00
							Acris Antibodies GmbH

CRISPR

Catalog No.	Species	Donor Vector
KN307154	Gpc1 - mouse gene knockout kit via CRISPR
	Mouse	GFP-Puro	1 kit / €1,200.00
			OriGene Technologies, Inc.
KN307154BN	Gpc1 - mouse gene knockout kit via CRISPR
	Mouse	mBFP-Neo	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN307154LP	Gpc1 - mouse gene knockout kit via CRISPR
	Mouse	Luciferase-Puro	1 kit / €1,290.00
			OriGene Technologies, Inc.
KN307154RB	Gpc1 - mouse gene knockout kit via CRISPR
	Mouse	RFP-BSD	1 kit / €1,290.00
			OriGene Technologies, Inc.

Lysates

Catalog No.
LY419549	GPC1 overexpression lysate
		0.1 mg / €280.00
		OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.
RC208602L2V	Lenti ORF particles, GPC1 (mGFP-tagged) - Human glypican 1 (GPC1), 200 uL, >10^7 TU/mL
		200 µl / €1,050.00
		OriGene Technologies, Inc.

RC208602L1V	Lenti ORF particles, GPC1 (Myc-DDK tagged) - Human glypican 1 (GPC1) , 200 uL, >10^7 TU/mL
		200 µl / €940.00
		OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.
MR208830L2V	Lenti ORF particles, Gpc1 (GFP-tagged) - Mouse glypican 1 (Gpc1), 200 uL, >10^7 TU/mL
		200 µl / €1,190.00
		OriGene Technologies, Inc.

MR208830L1V	Lenti ORF particles, Gpc1 (Myc-DDK-tagged) - Mouse glypican 1 (Gpc1), 200 uL, >10^7 TU/mL
		200 µl / €1,070.00
		OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.
RR207143L2V	Lenti ORF particles, Gpc1 (GFP-tagged ORF) - Rat glypican 1 (Gpc1), 200 uL, >10^7 TU/mL
		200 µl / €1,200.00
		OriGene Technologies, Inc.

RR207143L1V	Lenti ORF particles, Gpc1 (Myc-DDK-tagged ORF) - Rat glypican 1 (Gpc1), 200 uL, >10^7 TU/mL
		200 µl / €1,071.00
		OriGene Technologies, Inc.

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