GM-CSF antibody

Principal name

GM-CSF antibody

Alternative names for GM-CSF antibody

CSF2, GMCSF, Sargramostim, Molgramostin, Granulocyte-macrophage colony-stimulating factor, Colony stimulating factor 2

SwissProt ID

O62757 (Felca), P01587 (Mouse), P04141 (Human), P11052 (Bovin), P28773 (Sheep), P48749 (Canfa), P48750 (Rat), Q29118 (Pig), Q60481 (Cavpo)

Gene ID

1437 (CSF2), 116630 (Csf2), 12981, 281095, 443400

Available reactivities

Hu (Human), Bov (Bovine), Rt (Rat), Ms (Mouse), Macaque, Sh (Sheep), Mky (Monkey)

Available hosts

Mouse, Rat, Rabbit, Chicken, Sheep

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Functional assay (FN), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Flow Cytometry (F), Radioimmunoassay (R), Neutralisation (NEUT), Paraffin Sections (P), ELISA (capture) (E(capture)), Immunoprecipitation (IP)

Background of GM-CSF antibody

Granulocyte-macrophage colony stimulating factor (GM-CSF) regulates hematopoiesis, cell migration and immunity. GM-CSF is crucial for the growth and development of granulocyte and macrophage progenitor cells particularly during host defence and inflammatory reactions. GM-CSF is glycosylated at two N-linked and several O-linked sites. Glycosylation of GMCSF is important as it can affect pharmacokinetics, receptor affinity, biological activity, in vivo clearance rates and immunoreactivity. Patients receiving human recombinant GM-CSF commonly develop antibodies that recognize recombinant human GM-CSF produced in yeast (sargramostim) and E. coli (molgramostin), which exhibit glycosylation patterns that are distinct to the native human glycosylation. This is a HCX protein. HCX Expression System Details HCX proteins mimic the proteins in the human body because they are expressed from human, rather than animal, insect or bacterial cells. This process gives them human post-translational modifications. Recombinant DNA techniques allow a human protein with the correct amino acid sequence to be expressed in a non-human cell line. However, non-human cells lack the appropriate cellular machinery, such as specific glycosyltransferases, necessary to produce the correct human post-translational modifications of a protein. An extreme example is seen in E. coli cells, which produce recombinant proteins with no glycosylation, as the above figure illustrates. Rodent and yeast cells are able to glycosylate proteins, but they are still different from glycosylation in human cells. Expression System Resultant Proteins Human (e.g. K562, HEK293) Correct amino acid sequence Human post-translational modifications Rodent (e.g. CHO, NSO) Correct amino acid sequence Some natural glycosylation - not human-like Yeast (e.g. Pichia) Correct amino acid sequence Some natural glycosylation - not human-like E.Coli Correct amino acid sequence No PTMs Although there have been significant attempts to make non-human cell derived cytokines more human-like, there is a growing awareness that in many instances, particularly in therapeutics, cytokines should mimic those found in the body as closely as possible.

General readings

MartinezMoczygemba,M. and D.P. Huston (2003) J. Allergy Clin. Immunol. 112:653.

Barreda, D.R. et al. (2004) Dev. Comp. Immunol. 28:509.

Eksioglu, E.A. et al. (2007) Exp. Hematol. 35:1163.

Cao, Y. (2007) J. Clin. Invest. 117:2362.

Fleetwood, A.J. et al. (2005) Crit. Rev. Immunol. 25:405.

Heuser, M. et al. (2007) Semin. Hematol. 44:148.

Hege, K.M. et al. (2006) Int. Rev. Immunol. 25:321.

Kaushansky, K. et al. (1992) Biochemistry 31:1881.

Diederichs, K. et al. (1991) Science 254:1779.

Smith, L.R. et al. (1994) Immunogenetics 39:80.

OnettoPothier,N. et al. (1990) Blood 75:59.

Hayashida, K. et al. (1990) Proc. Natl. Acad. Sci. 87:9655.

Pelley, J.L. et al. (2007) Exp. Hematol. 35:1483.

Oaks, M.K. et al. (1995) J. Interferon Cytokine Res. 15:1095.

Vandenabeele, P. et al. (1990) Lymphokine Res. 9:381.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GM-CSF antibody

  CSF2 mouse monoclonal antibody, clone OTI1A3 (formerly 1A3)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human lung tissue using anti-CSF2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA808006)(1:150) Mouse IgG2b OTI1A3 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GM-CSF antibody

  CSF2 mouse monoclonal antibody, clone OTI8G5 (formerly 8G5)  
Immunohistochemical staining of paraffin-embedded Carcinoma of Human liver tissue using anti-CSF2 mouse monoclonal antibody.(Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808009)(1:150) Mouse IgG1 OTI8G5 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GM-CSF (Center) antibody

CSF2 antibody (Center) (Cat. #AP51097PU-N) immunohistochemistry analysis in formalin fixed and paraffin embedded human lung carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CSF2 antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated. Rabbit Ig Aff - Purified Hu F, ICC/IF, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

GM-CSF antibody

Formalin-Fixed, Paraffin-Embedded Sections of human cervical squamous cell carcinoma stained with GM-CSF Antibody Cat.-No PP1101P . Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. Rabbit Aff - Purified Hu E, FN, P, WB
50 µg / €230.00
  Acris Antibodies GmbH

GM-CSF antibody

Formalin-Fixed, Paraffin-Embedded Sections of human cervical squamous cell carcinoma stained with GM-CSF Antibody Cat.-No PP1101P . Tissue samples were provided by the Cooperative Human Tissue Network, which is funded by the National Cancer Institute. Rabbit Aff - Purified Hu E, FN, P, WB
0.1 mg / €340.00
  Acris Antibodies GmbH

GM-CSF antibody

  Rabbit polyclonal anti-GM-CSF antibody  
Western blot analysis of GMCSF using recombinant human GM-CSF. Rabbit Purified Hu NEUT, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GM-CSF antibody

  Rabbit polyclonal anti-GM-CSF antibody  
WB using anti-Human GM-CSF antibody shows detection of a band ~15 kDa in size corresponding to recombinant human GM-CSF (lane 1). Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using DyLight™649 conjugated anti-Rabbit IgG secondary antibody diluted 1:20,000 in blocking buffer . Rabbit IgG Purified Hu E, NEUT, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

GM-CSF antibody

  Rabbit polyclonal anti-GM-CSFantibody  
WB using anti-Mouse GM-CSF antibody shows detection of a band ~14 kDa in size corresponding to recombinant mouse GM-CSF (lane 1). Molecular weight markers are also shown (M). After transfer, the membrane was blocked overnight with 3% BSA in TBS followed by reaction with primary antibody at a 1:1,000 dilution. Detection occurred using DyLight™649 conjugated anti-Rabbit IgG secondary antibody diluted 1:20,000 in blocking buffer. Rabbit IgG Purified Ms E, NEUT, WB
0.1 mg / €356.00
  OriGene Technologies, Inc.

GM-CSF antibody

  Anti-CSF2 Rabbit Polyclonal Antibody  
Predicted band size: 16 kDa . Positive control: NIH/3T3 cell lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/600 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 5 seconds) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GM-CSF antibody

  Biotinylated Anti-Human GM-CSF Rabbit Polyclonal Antibody  
To detect hGM-CSF by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant hGM-CSF. Rabbit IgG Purified Hu E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

GM-CSF antibody

  Anti-Human GM-CSF Rabbit Polyclonal Antibody  
To detect hGM-CSF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human GM-CSF (TA328327) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hGM-CSF. Rabbit IgG Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GM-CSF antibody

  Biotinylated Anti-Murine GM-CSF Rabbit Polyclonal Antibody  
To detect mGM-CSF by direct ELISA (using 100 ul/well antibody solution) a concentration of 0.25-1.0 ug/ml of this antibody is required.  This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2-0.4 ng/well of recombinant mGM-CSF. Rabbit IgG Purified E, WB
50 µg / €325.00
  OriGene Technologies, Inc.

GM-CSF antibody

  Anti-Murine GM-CSF Rabbit Polyclonal Antibody  
To detect mGM-CSF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine GM-CSF (TA328516) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mGM-CSF. Rabbit IgG Purified E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GM-CSF antibody

  Rabbit Polyclonal GM-CSF Antibody  
ELISA: GM-CSF Antibody - Affinity Purified Rabbit IgG Purified Hu E
0.1 mg / €325.00
  OriGene Technologies, Inc.

GM-CSF antibody

  CSF2 mouse monoclonal antibody, clone OTI8F1 (formerly 8F1)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CSF2 (RC211109, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CSF2.(1:2000) Mouse IgG1 OTI8F1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GM-CSF antibody

  CSF2 mouse monoclonal antibody, clone OTI6H1 (formerly 6H1)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CSF2 (RC211109, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CSF2.(1:2000) Mouse IgG1 OTI6H1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GM-CSF antibody

  CSF2 mouse monoclonal antibody, clone OTI13E6 (formerly 13E6)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CSF2 (RC211109, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CSF2.(1:2000) Mouse IgG1 OTI13E6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GM-CSF antibody

  CSF2 mouse monoclonal antibody, clone OTI4B2 (formerly 4B2)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CSF2 (RC211109, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CSF2.(1:2000) Mouse IgG1 OTI4B2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GM-CSF antibody

GM-CSF Mouse IgG1 30-4 Purified Hu C, E, FN, WB
0.1 mg / €490.00
  Acris Antibodies GmbH

GM-CSF antibody

GM-CSF Rat IgG2a BVD2-23B6 Purified Hu C, E
0.5 mg / €450.00
  Acris Antibodies GmbH

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