GNAT3 antibody

Principal name

GNAT3 antibody

Alternative names for GNAT3 antibody

Gustducin alpha-3 chain, G protein G(t) subunit alpha-3, Guanine nucleotide-binding protein G(t) subunit alpha-3

SwissProt ID

A8MTJ3 (Human), P0C7Q4 (Bovin), P29348 (Rat), Q3V3I2 (Mouse)

Gene ID

346562 (GNAT3), 242851 (Gnat3), 286924

Available reactivities

Hu (Human), Can (Canine), Ms (Mouse), Rt (Rat)

Available hosts

Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Flow Cytometry (F), Paraffin Sections (P)

Background of GNAT3 antibody

Guanine nucleotide-binding protein (G protein) alpha subunit playing a prominent role in bitter and sweet taste transduction as well as in umami (monosodium glutamate, monopotassium glutamate, and inosine monophosphate) taste transduction. Transduction by this alpha subunit involves coupling of specific cell-surface receptors with a cGMP-phosphodiesterase; Activation of phosphodiesterase lowers intracellular levels of cAMP and cGMP which may open a cyclic nucleotide-suppressible cation channel leading to influx of calcium, ultimately leading to release of neurotransmitter. Indeed, denatonium and strychnine induce transient reduction in cAMP and cGMP in taste tissue, whereas this decrease is inhibited by GNAT3 antibody. Gustducin heterotrimer transduces response to bitter and sweet compounds via regulation of phosphodiesterase for alpha subunit, as well as via activation of phospholipase C for beta and gamma subunits, with ultimate increase inositol trisphosphate and increase of intracellular Calcium. GNAT3 can functionally couple to taste receptors to transmit intracellular signal: receptor heterodimer TAS1R2/TAS1R3 senses sweetness and TAS1R1/TAS1R3 transduces umami taste, whereas the T2R family GPCRs act as bitter sensors. Functions also as lumenal sugar sensors in the gut to control the expression of the Na+-glucose transporter SGLT1 in response to dietaty sugar, as well as the secretion of Glucagon-like peptide-1, GLP-1 and glucose-dependent insulinotropic polypeptide, GIP. Thus, may modulate the gut capacity to absorb sugars, with implications in malabsorption syndromes and diet-related disorders including diabetes and obesity.

General readings

Fushan, A.A., et al. Chem. Senses 35(7):579-592(2010)
Luttrell, L.M. Mol. Biotechnol. 39(3):239-264(2008)
Margolskee, R.F., et al. Proc. Natl. Acad. Sci. U.S.A. 104(38):15075-15080(2007)
Jang, H.J., et al. Proc. Natl. Acad. Sci. U.S.A. 104(38):15069-15074(2007)
Fehr, J., et al. J. Comp. Physiol. A Neuroethol. Sens. Neural. Behav. Physiol. 193(1):21-34(2007)

19 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GNAT3 antibody

  GNAT3 / Gustducin Goat Polyclonal (Internal) Antibody  
Anti-GNAT3 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml. Goat Purified Can, Hu, Ms, Rt E, P
50 µg / €400.00
  OriGene Technologies, Inc.

GNAT3 antibody

  Rabbit Polyclonal Anti-GNAT3 Antibody  
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane 1-2: Mouse heart tissue, Mouse muscle tissue, Primary antibody: (GNAT3 Antibody) at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 1 minute Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GNAT3 (Center) antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma reacted with GNAT3 Antibody (Center) Cat.-No AP51877PU-N, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Aff - Purified Hu F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

GNAT3 - human gene knockout kit via CRISPR

GNAT3 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

GNAT3 - human gene knockout kit via CRISPR

GNAT3 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

GNAT3 - human gene knockout kit via CRISPR

GNAT3 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

GNAT3 - human gene knockout kit via CRISPR

GNAT3 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Gnat3 - mouse gene knockout kit via CRISPR

Gnat3 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Gnat3 - mouse gene knockout kit via CRISPR

Gnat3 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Gnat3 - mouse gene knockout kit via CRISPR

Gnat3 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Gnat3 - mouse gene knockout kit via CRISPR

Gnat3 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

GNAT3

  Recombinant protein of human guanine nucleotide binding protein, alpha transducing 3 (GNAT3)  
GNAT3 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

GNAT3 overexpression lysate

GNAT3 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, GNAT3 (mGFP-tagged) - Human guanine nucleotide binding protein, alpha transducing 3 (GNAT3), 200 uL, >10^7 TU/mL

Lenti ORF particles, GNAT3 (mGFP-tagged) - Human guanine nucleotide binding protein, alpha transducing 3 (GNAT3), 200 uL, >10^7 TU/mL  
200 µl / €920.00
  OriGene Technologies, Inc.

Lenti ORF particles, GNAT3 (Myc-DDK tagged) - Human guanine nucleotide binding protein, alpha transducing 3 (GNAT3) , 200 uL, >10^7 TU/mL

Lenti ORF particles, GNAT3 (Myc-DDK tagged) - Human guanine nucleotide binding protein, alpha transducing 3 (GNAT3) , 200 uL, >10^7 TU/mL  
200 µl / €820.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Gnat3 (GFP-tagged) - Mouse guanine nucleotide binding protein, alpha transducing 3 (Gnat3), 200 uL, >10^7 TU/mL

Lenti ORF particles, Gnat3 (GFP-tagged) - Mouse guanine nucleotide binding protein, alpha transducing 3 (Gnat3), 200 uL, >10^7 TU/mL  
200 µl / €990.00
  OriGene Technologies, Inc.

Lenti ORF particles, Gnat3 (Myc-DDK-tagged) - Mouse guanine nucleotide binding protein, alpha transducing 3 (Gnat3), 200 uL, >10^7 TU/mL

Lenti ORF particles, Gnat3 (Myc-DDK-tagged) - Mouse guanine nucleotide binding protein, alpha transducing 3 (Gnat3), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Gnat3 (GFP-tagged ORF) - Rat guanine nucleotide binding protein, alpha transducing 3 (Gnat3), 200 uL, >10^7 TU/mL

Lenti ORF particles, Gnat3 (GFP-tagged ORF) - Rat guanine nucleotide binding protein, alpha transducing 3 (Gnat3), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, Gnat3 (Myc-DDK-tagged ORF) - Rat guanine nucleotide binding protein, alpha transducing 3 (Gnat3), 200 uL, >10^7 TU/mL

Lenti ORF particles, Gnat3 (Myc-DDK-tagged ORF) - Rat guanine nucleotide binding protein, alpha transducing 3 (Gnat3), 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

19 Item(s)

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