GNAT3 antibody

Principal name

GNAT3 antibody

Alternative names for GNAT3 antibody

Gustducin alpha-3 chain, G protein G(t) subunit alpha-3, Guanine nucleotide-binding protein G(t) subunit alpha-3

SwissProt ID

A8MTJ3 (Human), P0C7Q4 (Bovin), P29348 (Rat), Q3V3I2 (Mouse)

Gene ID

346562 (GNAT3), 242851 (Gnat3)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Bov (Bovine), Eq (Equine), Hst (Hamster), Mky (Monkey), Por (Porcine), Rb (Rabbit), Gorilla

Available hosts

Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Frozen Sections (C), Paraffin Sections (P), Flow Cytometry (F)

Background of GNAT3 antibody

Function: Guanine nucleotide-binding protein (G protein) alpha subunit playing a prominent role in bitter and sweet taste transduction as well as in umami (monosodium glutamate, monopotassium glutamate, and inosine monophosphate) taste transduction. Transduction by this alpha subunit involves coupling of specific cell-surface receptors with a cGMP-phosphodiesterase; Activation of phosphodiesterase lowers intracellular levels of cAMP and cGMP which may open a cyclic nucleotide-suppressible cation channel leading to influx of calcium, ultimately leading to release of neurotransmitter. Indeed, denatonuim and strychnine induce transient reduction in cAMP and cGMP in taste tissue, whereas this decrease is inhibited by GNAT3 antibody. Gustducin heterotrimer transduces response to bitter and sweet compounds via regulation of phosphodiesterase for alpha subunit, as well as via activation of phospholipase C for beta and gamma subunits, with ultimate increase inositol trisphosphate and increase of intracellular Calcium. GNAT3 can functionally couple to taste receptors to transmit intracellular signal: receptor heterodimer TAS1R2/TAS1R3 senses sweetness and TAS1R1/TAS1R3 transduces umami taste, whereas the T2R family GPCRs act as bitter sensors. Functions also as lumenal sugar sensors in the gut to control the expression of the Na+-glucose transporter SGLT1 in response to dietaty sugar, as well as the secretion of Glucagon-like peptide-1, GLP-1 and glucose-dependent insulinotropic polypeptide, GIP. Thus, may modulate the gut capacity to absorb sugars, with implications in malabsorption syndromes and diet-related disorders including diabetes and obesity.; Subcellular location: cytoplasm; Tissue specificity: Expressed in taste buds (sensory organs of clustered epithelial cells) of the circumvallate and fungiform papillae of the tongue as well as in palatal taste buds at protein level. Expressed in enteroendocrine cells of the gut, such as in subsets of enteroendocrine cells in the midjejunum and brush cells. Detected also in spermatozoa.

General readings

Fushan, A.A., et al. Chem. Senses 35(7):579-592(2010)
Luttrell, L.M. Mol. Biotechnol. 39(3):239-264(2008)
Margolskee, R.F., et al. Proc. Natl. Acad. Sci. U.S.A. 104(38):15075-15080(2007)
Jang, H.J., et al. Proc. Natl. Acad. Sci. U.S.A. 104(38):15069-15074(2007)
Fehr, J., et al. J. Comp. Physiol. A Neuroethol. Sens. Neural. Behav. Physiol. 193(1):21-34(2007)

5 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GNAT3 antibody

Anti-GNAT3 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml. Goat Purified Can, Hu, Ms, Rt E, P
50 µg / €345.00
  OriGene Technologies, Inc.

GNAT3 (Center) antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma reacted with GNAT3 Antibody (Center) Cat.-No AP51877PU-N, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Aff - Purified Hu F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

GNAT3 antibody

GNAT3 Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

GNAT3

GNAT3 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

GNAT3 overexpression lysate

GNAT3 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

5 Item(s)

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