GNE / GLCNE antibody

Principal name

GNE / GLCNE antibody

Alternative names for GNE / GLCNE antibody

UDP-GlcNAc-2-epimerase/ManAc kinase, * Bifunctional UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase

SwissProt ID

O35826 (Rat), Q7TQ49 (Crigr), Q91WG8 (Mouse), Q9Y223 (Human)

Gene ID

10020 (GNE)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P)

Background of GNE / GLCNE antibody

The bifunctional enzyme UDP-N-acetylglucosamine 2-epimerase/N-acetylmannosamine kinase (GNE/Mnk), or GLCNE, regulates and initiates biosynthesis of N-acetylneuraminic acid (NeuAc), a precursor of sialic acids. GLCNE is required for normal sialylation in hematopoietic cells. Sialylation is implicated in cell adhesion, signal transduction, tumorigenicity and metastatic behavior of malignant cells. It is upregulated after PKC-dependent phosphorylation and is most abundantly expressed in liver and placenta. It is also expressed, to a lesser extent, in heart, brain, lung, kidney, skeletal muscle and pancreas. Defects in GLCNE are the cause of sialuria, inclusion body myopathy 2 (IBM2) and Nonaka myopathy (NM) or distal myopathy with rimmed vacuoles (DMRV). Sialuria is an autosomal dominant disorder caused by a lack of feedback inhibition of GLCNE by CMP-NeuAc, resulting in overproduction of NeuAc. It is characterized by an accumulation of free sialic acid in the cytoplasm and large quantities of neuraminic acid in the urine. Both IBM2 and NM/DMRV are autosomal recessive neuromuscular disorders characterized by adult onset, distal and proximal muscle weakness (especially in the legs) and a typical muscle pathology including filamentous inclusions and rimmed vacuoles.

General readings

Stober, A., et al. Neuromuscul. Disord. 20(5):335-336(2010)
Reinke, S.O., et al. Glycoconj. J. 26(4):415-422(2009)
Tong, Y., et al. PLoS ONE 4 (10), E7165 (2009):
Reinke, S.O., et al. FEBS Lett. 581(17):3327-3331(2007)
Watts, G.D., et al. Neuromuscul. Disord. 13 (7-8), 559-567 (2003)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GNE / GLCNE antibody

Western blot analysis of extracts from NIH/3T3 cells, using GNE antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GNE / GLCNE (N-term) antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue reacted with GNE / GLCNE Antibody (N-term) Cat.-No AP51881PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. Rabbit Ig Aff - Purified Hu P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

GNE / GLCNE antibody

GNE / GLCNE Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

GNE / GLCNE antibody

Western Blot analysis of GNE expression in transfected 293T cell line (H00010020-T01) by GNE MaxPab polyclonal antibody.

Lane 1: GNE transfected lysate(79.30 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

GNE / GLCNE antibody

Immunohistochemistry-Paraffin: GNE Antibody [NBP1-81621] - Immunohistochemical staining of human prostate shows strong cytoplasmic positivity in glandular cells. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

GNE / GLCNE antibody

Immunohistochemistry-Paraffin: GNE Antibody [NBP1-81622] Immunohistochemical staining of human liver shows strong cytoplasmic and nuclear positivity in hepatocytes. Rabbit IgG Aff - Purified Hu P
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

GNE / GLCNE (transcript variant 2)

GNE / GLCNE Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

GNE / GLCNE

GNE / GLCNE Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue
10 µg / €380.00
  Abnova Taiwan Corp.

GNE / GLCNE

GNE / GLCNE Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue
25 µg / €510.00
  Abnova Taiwan Corp.

GNE / GLCNE

GNE / GLCNE Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

GNE / GLCNE

GNE / GLCNE Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

GNE overexpression lysate

GNE overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

GNE overexpression lysate

GNE overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

13 Item(s)

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