GNMT antibody

Principal name

GNMT antibody

Alternative names for GNMT antibody

Glycine N-methyltransferase

SwissProt ID

P13255 (Rat), Q14749 (Human), Q29513 (Rabit), Q29555 (Pig), Q9QXF8 (Mouse)

Gene ID

27232 (GNMT), 14711 (Gnmt)

Available reactivities

Hu (Human), Ms (Mouse), Por (Porcine), Rt (Rat)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F)

Background of GNMT antibody

Glycine N-methyltransferase, also known as GNMT, catalyzes the synthesis of N-methylglycine (sarcosine) from glycine using S-adenosylmethionine (AdoMet) as the methyl donor. This protein affects DNA methylation by regulating the ratio of S-adenosylmethionine to S-adenosylhomocystine and participates in the detoxification pathway in liver cells. Also it is reported that GNMT expression is diminished in human hepatocellular carcinoma (HCC). Recombinant human GNMT protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

General readings

Liao YJ., et al. (2009) Int J Cancer.124(4):816-26.
Huang YC., et al. (2008) J Gastroenterol Hepatol. 23(9):1384-9.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GNMT antibody

Western blot analysis of extracts (10ug) from 7 different cell lines by using anti-GNMT monoclonal antibody.(1:200) Mouse IgG3 OTI8A3 Purified Hu F, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

GNMT antibody

The Recombinant protein (50ng) and Tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GNMT antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. GNMT recombinant protein. Lane 2: Liver tissue lysate.The Cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GNMT antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: 293T cell lysate, Lane 2: GNMT Transfected 293T cell lysate. Mouse IgG2a AT5D1 Purified Hu E, ICC/IF, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

GNMT antibody

The Recombinant protein (50ng) and Tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GNMT antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1. GNMT recombinant protein. Lane 2: Liver tissue lysate.The Cell lysates (10ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human GNMT antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1: 293T cell lysate, Lane 2: GNMT Transfected 293T cell lysate. Mouse IgG2a AT5D1 Purified Hu E, ICC/IF, WB
50 µl / €220.00
  Acris Antibodies GmbH

GNMT antibody

GNMT MaxPab polyclonal antibody. Western Blot analysis of GNMT expression in human liver. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

GNMT antibody

Western Blot analysis of GNMT expression in transfected 293T cell line (H00027232-T03) by GNMT MaxPab polyclonal antibody.

Lane 1: GNMT transfected lysate(32.70 KDa).
Lane 2: Non-transfected lysate. Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

GNMT antibody

Western Blot analysis of GNMT expression in transfected 293T cell line (H00027232-T03) by GNMT MaxPab polyclonal antibody.

Lane 1: GNMT transfected lysate(32.70 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

GNMT antibody

GNMT Mouse IgM 2B10 Ascites Hu E
0.2 ml / €370.00
  Abnova Taiwan Corp.

GNMT (N-term) antibody

Mouse Small Intestine; WB Suggested Anti-Gnmt Antibody. . Titration: 1.0 ug/ml. . Positive Control: Mouse Small Intestine; Gnmt antibody - N-terminal region (ARP43564_P050) in Mouse Small Intestine cells using Western Blot Rabbit Aff - Purified Ms
50 µg / €350.00
  AVIVA Systems Biology

GNMT (C-term) antibody

Mouse Liver; WB Suggested Anti-Gnmt Antibody. . Titration: 1.0 ug/ml. . Positive Control: Mouse Liver; Gnmt antibody - C-terminal region (ARP43565_P050) in Mouse Liver cells using Western Blot Rabbit Aff - Purified Ms
50 µg / €350.00
  AVIVA Systems Biology

GNMT antibody

Immunohistochemistry-Paraffin: GNMT Antibody [NBP1-89512] - Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

GNMT

GNMT Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

GNMT

GNMT Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
10 µg / €299.00
  OriGene Technologies, Inc.

GNMT (1-295, His-tag)

GNMT Human Purified > 95 % by SDS PAGE E. coli
0.5 mg / €540.00
  Acris Antibodies GmbH

GNMT (1-295, His-tag)

GNMT Human Purified > 95 % by SDS PAGE E. coli
0.1 mg / €230.00
  Acris Antibodies GmbH

GNMT

GNMT Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

GNMT

GNMT Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

GNMT

GNMT Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

GNMT

GNMT Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

GNMT

SDS-Page: GNMT Protein [NBC1-18532] - GNMT, 34.9kDa (315aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE Protein
0.1 mg / €310.00
  Novus Biologicals Inc.

Antibody Pairs

Catalog No.    

GNMT (Human) IP-WB Antibody Pair

GNMT (Human) IP-WB Antibody Pair  
1 set / €710.00
  Abnova Taiwan Corp.

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