Golgi phosphoprotein 3 / GOLPH3 antibody

Principal name

Golgi phosphoprotein 3 / GOLPH3 antibody

Alternative names for Golgi phosphoprotein 3 / GOLPH3 antibody

GPP34, Coat protein GPP34, MIDAS, Mitochondrial DNA absence factor

SwissProt ID

Q9CRA5 (Mouse), Q9ERE4 (Rat), Q9H4A6 (Human)

Gene ID

64083 (GOLPH3), 3640751 (ADH2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Bov (Bovine)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), Flow Cytometry (F), Frozen Sections (C)

Background of Golgi phosphoprotein 3 / GOLPH3 antibody

GOLPH3 was initially identified as a peripheral membrane protein localized to the trans-Golgi network, but others reported it to be primarily a mitochondrial protein that regulated the mitochondrial mass through the regulation of the mitochondria-specific phospholipid cardiolipin. GOLPH3 has since been implicated in the target of rapamycin (TOR) signalling pathway. Its overexpression in transfected cells led to and increase in anchorage-independent growth and cell proliferation in vitro. Furthermore, GOLPH3-transfected cells enhanced S6 Kinase activity in response to growth factor stimulation by EGF. Simultaneously, AKT phosphorylation increased in these cells, while these events were abrogated in GOLPH3 siRNA treated cells compared to control cells, indicating the GOLPH3 can enhance signalling through TOR-associated complexes. These results suggest that GOLPH3 is a bona fide oncogene and may be a useful target for therapeutic strategies.

17 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Golgi phosphoprotein 3 / GOLPH3 antibody

Western blot analysis of GOLPH3 in rat lung tissue lysate with GOLPH3 antibody at (A) 0.5 and (B) 1 µg/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Golgi phosphoprotein 3 / GOLPH3 antibody

GOLPH3 Antibody (C-term)(Cat. #TA324910) western blot analysis in 293,MDA-MB-231 cell line lysates (35ug/lane).This demonstrates the GOLPH3 antibody detected the GOLPH3 protein (arrow). Mouse IgG1 905CT9.1.1 Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Golgi phosphoprotein 3 / GOLPH3 antibody

GOLPH3 Antibody (C-term)(Cat. #TA325177) western blot analysis in HepG2,293 cell line lysates (35ug/lane).This demonstrates the GOLPH3 antibody detected the GOLPH3 protein (arrow). Mouse IgG1 905CT9.1.1 Ascites Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Golgi phosphoprotein 3 / GOLPH3 antibody

TA326322 (0.3µg/ml) staining of Human Platelets lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Can, Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Golgi phosphoprotein 3 / GOLPH3 (N-term) antibody

Western blot analysis of GOLPH3 antibody (N-term) in Hela cell line lysates (35ug/lane). GOLPH3 (arrow) was detected using the purified Pab. Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Golgi phosphoprotein 3 / GOLPH3 (C-term) antibody

Western blot analysis of anti-GOLPH3 Antibody (C-term) in mouse lung tissue lysates (35ug/lane). GOLPH3 (arrow) was detected using the purified Pab. Rabbit Ig Purified Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Golgi phosphoprotein 3 / GOLPH3 (N-term) antibody

Human Prostate: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Purified Hu, Ms, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Golgi phosphoprotein 3 / GOLPH3 Control Peptide

Golgi phosphoprotein 3 / GOLPH3
50 µg / €160.00
  Acris Antibodies GmbH

Golgi phosphoprotein 3 / GOLPH3 antibody

Western Blot analysis of GOLPH3 expression in transfected 293T cell line (H00064083-T02) by GOLPH3 MaxPab polyclonal antibody.

Lane 1: GOLPH3 transfected lysate(32.78 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Golgi phosphoprotein 3 / GOLPH3 antibody

Golgi phosphoprotein 3 / GOLPH3 Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €480.00
  Novus Biologicals Inc.

Golgi phosphoprotein 3 / GOLPH3 antibody

Western Blot: GOLPH3 Antibody [NBP1-76241] - Analysis of GOLPH3 in rat lung tissue lysate with GOLPH3 antibody at (A) 0.5 and (B) 1 ug/mL._x000d_ Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €440.00
  Novus Biologicals Inc.

Golgi phosphoprotein 3 / GOLPH3 antibody

Immunohistochemistry-Paraffin: GOLPH3 Antibody [NBP1-81804] - Immunohistochemical staining of human kidney shows strong cytoplasmic positivity in distal tubules. Rabbit IgG Aff - Purified Hu ICC/IF, P
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Golgi phosphoprotein 3 / GOLPH3

Golgi phosphoprotein 3 / GOLPH3 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Golgi phosphoprotein 3 / GOLPH3

Golgi phosphoprotein 3 / GOLPH3 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

Golgi phosphoprotein 3 / GOLPH3

Golgi phosphoprotein 3 / GOLPH3 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

GOLPH3 293T Cell Transient Overexpression Lysate(Denatured)

GOLPH3 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

GOLPH3 overexpression lysate

GOLPH3 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

17 Item(s)

per page
  • LinkedIn