GOPC / PIST antibody

Principal name

GOPC / PIST antibody

Alternative names for GOPC / PIST antibody

CAL, FIG, Golgi-associated PDZ and coiled-coil motif-containing protein, PDZ protein interacting specifically with TC10, CFTR-associated ligand, Fused in glioblastoma

SwissProt ID

Q8BH60 (Mouse), Q9HD26 (Human)

Gene ID

57120 (GOPC), 94221 (Gopc)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Ze (Zebrafish), African clawed frog, GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Eq (Equine)

Available hosts

Rabbit, Goat

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of GOPC / PIST antibody

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components (1,2) and is negatively regulated by TOR (Target of rapamycin) (3). PIST, a PDZ-containing protein, was discovered in a yeast two-hybrid system as a binding partner to Beclin-1, a Bcl-2-interacting protein homologous to the yeast autophagy gene apg6 (4-6). Experiments with mutant PIST proteins lacking the PDZ domain showed that PIST interaction with Beclin-1 through its coiled-coil domain can modulate Beclin-1 activity and suggest that PIST interactions with other proteins through its PDZ domain may regulate the activity of PIST and Beclin-1 (6).

General readings

Neudauer CL, Joberty G, Macara IG. PIST: a novel PDZ/coiled-coil domain binding partner for the rho-family GTPase TC10. Biochem Biophys Res Commun. 2001 Jan 19;280(2):541-7.
PMID: 11162552

18 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GOPC / PIST (C-term) antibody

  Goat Anti-PIST / FIG / GOPC Antibody  
TA305733 (0.05ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GOPC / PIST (C-term) antibody

  Rabbit Polyclonal PIST Antibody  
Western blot analysis of PIST in rat colon cell lysate with PIST antibody at (A) 1, (B) 2 and (C) 4 ug/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GOPC / PIST (Center) antibody

  Rabbit Polyclonal PIST Antibody  
Western blot analysis of PIST in PC-3 cell lysate with PIST antibody at (A) 1, (B) 2 and (C) 4 ug/ml. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GOPC / PIST antibody

  Rabbit Polyclonal Anti-GOPC Antibody  
WB Suggested Anti-GOPC Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Hela cell lysate Rabbit IgG Purified Bov, Can, GP, Hu, Ms, Por, Rb WB
50 µg / €325.00
  OriGene Technologies, Inc.

GOPC / PIST antibody

  Rabbit Polyclonal Anti-GOPC Antibody  
WB Suggested Anti-GOPC Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Hela cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por WB
50 µg / €325.00
  OriGene Technologies, Inc.

GOPC / PIST antibody

Brain, cerebellum: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu, Ms, Rt P, WB
50 µg / €470.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

Gopc - mouse gene knockout kit via CRISPR

Gopc - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Gopc - mouse gene knockout kit via CRISPR

Gopc - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Gopc - mouse gene knockout kit via CRISPR

Gopc - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Gopc - mouse gene knockout kit via CRISPR

Gopc - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

GOPC / PIST (transcript variant 1)

  Recombinant protein of human golgi associated PDZ and coiled-coil motif containing (GOPC), transcript variant 1  
GOPC / PIST Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

GOPC / PIST (278-454, His-tag)

GOPC / PIST Human Purified > 90 % by SDS – PAGE E. coli
0.5 mg / €670.00
  Acris Antibodies GmbH

GOPC / PIST (278-454, His-tag)

GOPC / PIST Human Purified > 90 % by SDS – PAGE E. coli
0.1 mg / €250.00
  Acris Antibodies GmbH

Lysates

Catalog No.    

GOPC overexpression lysate

GOPC overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, GOPC (mGFP-tagged)-Human golgi-associated PDZ and coiled-coil motif containing (GOPC), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, GOPC (mGFP-tagged)-Human golgi-associated PDZ and coiled-coil motif containing (GOPC), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, GOPC (Myc-DDK-tagged)-Human golgi-associated PDZ and coiled-coil motif containing (GOPC), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, GOPC (Myc-DDK-tagged)-Human golgi-associated PDZ and coiled-coil motif containing (GOPC), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Gopc (GFP-tagged) - Mouse golgi associated PDZ and coiled-coil motif containing (Gopc), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, Gopc (GFP-tagged) - Mouse golgi associated PDZ and coiled-coil motif containing (Gopc), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €1,060.00
  OriGene Technologies, Inc.

Lenti ORF particles, Gopc (Myc-DDK-tagged) - Mouse golgi associated PDZ and coiled-coil motif containing (Gopc), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, Gopc (Myc-DDK-tagged) - Mouse golgi associated PDZ and coiled-coil motif containing (Gopc), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €950.00
  OriGene Technologies, Inc.

18 Item(s)

per page
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