GORASP2 / GOLPH6 antibody

Principal name

GORASP2 / GOLPH6 antibody

Alternative names for GORASP2 / GOLPH6 antibody

Golgi reassembly-stacking protein 2, GRS2, Golgi phosphoprotein 6, p59, Golgi marker, Golgi reassembly-stacking protein of 55 kDa

SwissProt ID

Q99JX3 (Mouse), Q9H8Y8 (Human), Q9R064 (Rat)

Gene ID

26003 (GORASP2), 70231 (Gorasp2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P)

Background of GORASP2 / GOLPH6 antibody

The Golgi apparatus is a highly complex organelle comprised of a stack of cisternal membranes on the secretory pathway from the ER to the cell surface. The structure is maintained by an exoskeleton or Golgi matrix constructed from a family of coiled-coil protein, the golgins and other peripheral membrane components such as GRASP55 and GRASP65. GRASP55 (Golgi reassembly stacking protien or p59) is a component of the Golgi stacking machinery. GRASP55 is highly homologous to GRASP65 and contains two PDZ domains. GRASP55 is myristoylated and palmitoylated. Unlike GRASP65, GRASP55 does not have detectable binding with the vesicle docking protein GM130 and is located on the medial-Golgi rather than cis-Golgi. Both GRASP55 and GRASP65 function in the stacking of Golgi cisternae. The novel coiled-coil protein golgin 45 interacts with GRASP55 and the GTP form of Rab 2, suggesting that GRASP55 and golgin 45 form a Rab 2 effector complex on medial-Golgi essential for normal protein transport and Golgi structure. ERK2 directly phosphorylates GRASP55, which is phosphorylated in mitotic cells, suggesting that mitogen-activated protein kinase kinase (MKK)/ERK pathway phosphorylates the Golgi during mitosis.

General readings

Kuo A. et al. (2000) EMBO J. 19:6427-6439.Truschel S.T.et al. (2011) J. Biol. Chem. 286:20125-20129.

16 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GORASP2 / GOLPH6 antibody

  Rabbit polyclonal anti-GORASP2 antibody  
Western blot analysis of extracts from HeLa cells, HepG2 cells, Jurkat cells and LOVO cells, using GORASP2 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GORASP2 / GOLPH6 antibody

  Rabbit Polyclonal Anti-GORASP2 Antibody  
Western blot analysis of GORASP2 expression in HeLa cell extracts Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GORASP2 / GOLPH6 antibody

Western blot (WB) analysis of GRASP55 antibody (Cat.-No.: AP01476PU-N) in extracts from LOVO cells. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €350.00
  Acris Antibodies GmbH

GORASP2 / GOLPH6 (Center) antibody

GORASP2 Antibody (Center) (Cat. #AP51894PU-N) western blot analysis in Jurkat cell line lysates (35µg/lane).This demonstrates the GORASP2 antibody detected the GORASP2 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

GORASP2 / GOLPH6 antibody

Western blot analysis of extracts from HeLa/HepG2/Jurkat/LOVO cells, using GORASP2 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €230.00
  Assay Biotech

GORASP2 / GOLPH6 antibody

Western blot analysis of extracts from HeLa/HepG2/Jurkat/LOVO cells, using GORASP2 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

GORASP2 - human gene knockout kit via CRISPR

GORASP2 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

GORASP2 - human gene knockout kit via CRISPR

GORASP2 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

GORASP2 - human gene knockout kit via CRISPR

GORASP2 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

GORASP2 - human gene knockout kit via CRISPR

GORASP2 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Gorasp2 - mouse gene knockout kit via CRISPR

Gorasp2 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

GORASP2 / GOLPH6 (full length, N-term HIS tag, transcript variant 1)

  Recombinant protein of human golgi reassembly stacking protein 2, 55kDa (GORASP2), transcript variant 1, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug  
GORASP2 / GOLPH6 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

GORASP2 / GOLPH6 (1-452, His-tag)

GORASP2 / GOLPH6 Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

GORASP2 / GOLPH6 (1-452, His-tag)

GORASP2 / GOLPH6 Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Gorasp2 (GFP-tagged) - Mouse golgi reassembly stacking protein 2 (Gorasp2), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, Gorasp2 (GFP-tagged) - Mouse golgi reassembly stacking protein 2 (Gorasp2), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, Gorasp2 (Myc-DDK-tagged) - Mouse golgi reassembly stacking protein 2 (Gorasp2), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, Gorasp2 (Myc-DDK-tagged) - Mouse golgi reassembly stacking protein 2 (Gorasp2), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

16 Item(s)

per page
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