GORASP2 / GOLPH6 antibody

Principal name

GORASP2 / GOLPH6 antibody

Alternative names for GORASP2 / GOLPH6 antibody

Golgi reassembly-stacking protein 2, GRS2, Golgi phosphoprotein 6, p59, Golgi marker, Golgi reassembly-stacking protein of 55 kDa

SwissProt ID

Q99JX3 (Mouse), Q9H8Y8 (Human), Q9R064 (Rat)

Gene ID

26003 (GORASP2), 70231 (Gorasp2), 2600321 (petB)

Available reactivities

Hu (Human), Mky (Monkey), Ms (Mouse), Rt (Rat), Bov (Bovine)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of GORASP2 / GOLPH6 antibody

The Golgi apparatus is a highly complex organelle comprised of a stack of cisternal membranes on the secretory pathway from the ER to the cell surface. The structure is maintained by an exoskeleton or Golgi matrix constructed from a family of coiled-coil protein, the golgins and other peripheral membrane components such as GRASP55 and GRASP65 (1). GRASP55 (Golgi reassembly stacking protien or p59) is a component of the Golgi stacking machinery. GRASP55 is highly homologous to GRASP65 and contains two PDZ domains. GRASP55 is myristoylated and palmitoylated. Unlike GRASP65, GRASP55 does not have detectable binding with the vesicle docking protein GM130 and is located on the medial-Golgi rather than cis-Golgi. Both GRASP55 and GRASP65 function in the stacking of Golgi Cisternae (2,3). The novel coiled-coil protein golgin 45 interacts with GRASP55 and the GTP form of Rab 2, suggesting that GRASP55 and golgin 45 form a Rab 2 effector complex on medial-Golgi essential for normal protein transport and Golgi structure (4). ERK2 directly phosphorylates GRASP55, which is phosphorylated in mitotic cells, suggesting that mitogen-activated protein kinase kinase (MKK)/ERK pathway phosphorylates the Golgi during mitosis (5).

General readings

Kuo A. et al. (2000) EMBO J. 19:6427-6439.Truschel S.T.et al. (2011) J. Biol. Chem. 286:20125-20129.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GORASP2 / GOLPH6 antibody

Western blot analysis of extracts from HeLa cells, HepG2 cells, Jurkat cells and LOVO cells, using GORASP2 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GORASP2 / GOLPH6 antibody

Western blot analysis of GORASP2 expression in HeLa cell extracts Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GORASP2 / GOLPH6 antibody

Western blot (WB) analysis of GRASP55 antibody (Cat.-No.: AP01476PU-N) in extracts from LOVO cells. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €350.00
  Acris Antibodies GmbH

GORASP2 / GOLPH6 (Center) antibody

GORASP2 Antibody (Center) (Cat. #AP51894PU-N) western blot analysis in Jurkat cell line lysates (35µg/lane).This demonstrates the GORASP2 antibody detected the GORASP2 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

GORASP2 / GOLPH6 antibody

GORASP2 / GOLPH6 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

GORASP2 / GOLPH6 antibody

Immunofluorescence of purified MaxPab antibody to GORASP2 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

GORASP2 / GOLPH6 antibody

Western blot analysis of extracts from HeLa/HepG2/Jurkat/LOVO cells, using GORASP2 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

GORASP2 / GOLPH6 antibody

Western blot analysis of extracts from HeLa/HepG2/Jurkat/LOVO cells, using GORASP2 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

GORASP2 / GOLPH6 (C-term) antibody

Human HepG2; WB Suggested Anti-GORASP2 Antibody. . Titration: 1.0 ug/ml. . Positive Control: HepG2 Whole Cell. GORASP2 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells.; GORASP2 antibody - C-terminal region (ARP62077_P050) in Human HepG2 cells using Western Blot Rabbit Aff - Purified Hu
50 µg / €350.00
  AVIVA Systems Biology

GORASP2 / GOLPH6 antibody

Immunohistochemical analysis of GRASP55 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Ms, Rt IP, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

GORASP2 / GOLPH6 antibody

Immunohistochemical analysis of GRASP55 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Ms, Rt IP, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

GORASP2 / GOLPH6 antibody

Immunohistochemical analysis of GRASP55 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Bov, Hu, Ms, Rt IP, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

GORASP2 / GOLPH6 antibody

Western Blot: GRASP55 Antibody [NB100-74431] - Figure 1 illustrates Western blot detection of GRASP55 protein (lane 1) by NB100-74431, and the neutralization of this detection using PEP-295 (lane 2). Rabbit IgG Aff - Purified Ms WB
0.1 mg / €400.00
  Novus Biologicals Inc.

GORASP2 / GOLPH6 antibody

Western Blot: GRASP55 Antibody [NBP1-00862] - Western blot (WB) analysis of GRASP55 (L215) antibody in extracts from LOVO cells. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €420.00
  Novus Biologicals Inc.

GORASP2 / GOLPH6 antibody

GORASP2 / GOLPH6 Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

GORASP2 / GOLPH6 antibody

GORASP2 / GOLPH6 Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
50 µg / €290.00
  Novus Biologicals Inc.

GORASP2 / GOLPH6 antibody

Immunohistochemistry-Paraffin: GRASP55 Antibody [NBP1-89747] -  staining of human rectum shows strong cytoplasmic positivity in granular pattern in glandular cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

GORASP2 / GOLPH6 (full length, N-term HIS tag, transcript variant 1)

GORASP2 / GOLPH6 Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

GORASP2 / GOLPH6 (1-452, His-tag)

GORASP2 / GOLPH6 Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

GORASP2 / GOLPH6 (1-452, His-tag)

GORASP2 / GOLPH6 Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €300.00
  Acris Antibodies GmbH

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