GOT1 antibody

Principal name

GOT1 antibody

Alternative names for GOT1 antibody

Aspartate aminotransferase, Transaminase A, Glutamate oxaloacetate transaminase 1

SwissProt ID

P00503 (Pig), P00504 (Chick), P05201 (Mouse), P13221 (Rat), P17174 (Human), P33097 (Bovin)

Gene ID

396967 (GOT1), 2805, 14718 (Got1)

Available reactivities

Por (Porcine), Hu (Human), Ms (Mouse), Bov (Bovine), Chk (Chicken), Rt (Rat), African clawed frog, Xen (Xenopus), Mky (Monkey), Chimp (Chimpanzee), Gt (Goat), Hst (Hamster), Gorilla

Available hosts

Sheep, Goat, Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), EPair for Elisa (EPair), Dot blot (Dot), Immunodiffusion (ID), Immunocytochemistry/Immunofluorescence (ICC/IF), Radioimmunoassay (R), Frozen Sections (C)

Background of GOT1 antibody

Aspartate aminotransferase (Glutamate oxaloacetate transaminase / GOT1) is a ubiquitous pyridoxal phosphate-dependent enzyme which exists in both mitochondrial and cytosolic forms. The enzyme plays an important role in amino acid metabolism and in the urea and tricarboxylic acid cycles. The 2 isoenzymes are homodimeric. In liver about 80% of the enzyme activity is mitochondrial in origin, whereas in serum the enzyme activity is largely cytosolic. Although the mitochondrial and soluble forms of GOT are coded by different chromosomes, the 2 show close homology in amino acid sequence and were presumably derived from a common ancestral gene.
Serum GOT (with SGPT) levels are usually elevated in states of hepatocellular injury (injury to the liver cells), the highest levels are associated with hepatitis of a viral origin. High levels are also found after myocardial infarction, when SGPT levels are lower.

General readings

Teichberg VI.
From the liver to the brain across the blood-brain barrier.
Proc Natl Acad Sci U S A. 2007 May 1;104(18):7315-6. Epub 2007 Apr 26. No
abstract available.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GOT1 antibody

Human Pineal Tissue; Rabbit Anti-GOT1 Antibody . Catalog Number: ARP48205_P050 . Formalin Fixed Paraffin Embedded Tissue: Human Pineal Tissue . Observed Staining: Cytoplasmic in cell bodies and processes of pinealocytes . Primary Antibody Concentration: 1:100 . Secondary Antibody: Donkey anti-Rabbit-Cy3 . Secondary Antibody Concentration: 1:200 . Magnification: 20X . Exposure Time: 0.5 - 2.0 sec .; Rabbit Anti-GOT1 Antibody (ARP48205_P050) ) in Human Pineal Tissue using Immunohistochemistry Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

GOT1 (22-35) antibody

TA303294 (0.5µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GOT1 (157-167) antibody

TA303313 (0.01µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GOT1 antibody

Anti-GOT1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. Goat IgG Aff - Purified Hu, Rt E, P, WB
50 µg / €345.00
  OriGene Technologies, Inc.

GOT1 antibody

Western blot analysis of extracts of various cell lines, using GOT1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

GOT1 (N-term) antibody

Western blot analysis of GOT1 Antibody (N-term) (AP18039PU-N) in mouse brain tissue lysates (35ug/lane). GOT1 (arrow) was detected using the purified Pab. Rabbit Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

GOT1 antibody

GOT1 Rabbit IgG Azide Free Por Dot, E, ICC/IF, ID, IP, R, WB
10 mg / €230.00
  Acris Antibodies GmbH

GOT1 antibody

GOT1 Rabbit IgG Biotin Por Dot, E, ICC/IF, ID, IP, R, WB
1 ml / €340.00
  Acris Antibodies GmbH

GOT1 antibody

GOT1 Rabbit IgG Aff - Purified Por Dot, E, ICC/IF, ID, IP, R, WB
1 mg / €430.00
  Acris Antibodies GmbH

GOT1 antibody

GOT1 Sheep Serum Por E, IP, WB
2 ml / €1,150.00
  Acris Antibodies GmbH

GOT1 antibody

GOT1 Sheep IgG Biotin Por E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

GOT1 antibody

GOT1 Sheep IgG HRP Por E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

GOT1 antibody

GOT1 Sheep Purified Por E, WB
0.1 mg / €440.00
  Acris Antibodies GmbH

GOT1 antibody

Western Blot analysis of GOT1 expression in transfected 293T cell line (H00002805-T01) by GOT1 MaxPab polyclonal antibody.

Lane 1: GOT1 transfected lysate(45.43 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

GOT1 antibody

Immunoperoxidase of purified MaxPab rabbit antibody to GOT1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml] Rabbit Serum Hu, Ms E(detection), IP, P, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

GOT1 antibody

Immunoperoxidase of purified MaxPab rabbit antibody to GOT1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml] Rabbit Purified Hu, Ms E(detection), P, WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

GOT1 antibody

GOT1 Mouse IgG2a 4G4 Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

GOT1 antibody

GOT1 polyclonal antibody ( Cat # PAB7390 ) ( 0.5 µg/ml ) staining of human liver lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

GOT1 antibody

GOT1 polyclonal antibody ( Cat # PAB7415 ) ( 0.01 µg/ml ) staining of human liver lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

GOT1 antibody

Hum. Fetal Liver; Host: Rabbit .  Target Name: GOT1 .  Sample Tissue: Human Fetal Liver . Antibody Dilution: 1.0ug/ml.; GOT1 antibody - N-terminal region (ARP48205_T100) in Hum. Fetal Liver cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Chk, Hu, Ms, Por, Rt WB
0.1 mg / €310.00
  AVIVA Systems Biology

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