GOT1 antibody

Principal name

GOT1 antibody

Alternative names for GOT1 antibody

Aspartate aminotransferase, Transaminase A, Glutamate oxaloacetate transaminase 1

SwissProt ID

P00503 (Pig), P00504 (Chick), P05201 (Mouse), P13221 (Rat), P17174 (Human), P33097 (Bovin)

Gene ID

396967 (GOT1), 2805, 14718 (Got1), 24401

Available reactivities

Por (Porcine), Hu (Human), Ms (Mouse), Bov (Bovine), Chk (Chicken), Rt (Rat), African clawed frog, Mky (Monkey)

Available hosts

Sheep, Goat, Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Immunoprecipitation (IP), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Paraffin Sections (P), EPair for Elisa (EPair), Dot blot (Dot), Immunodiffusion (ID), Immunocytochemistry/Immunofluorescence (ICC/IF), Radioimmunoassay (R)

Background of GOT1 antibody

Aspartate aminotransferase (Glutamate oxaloacetate transaminase / GOT1) is a ubiquitous pyridoxal phosphate-dependent enzyme which exists in both mitochondrial and cytosolic forms. The enzyme plays an important role in amino acid metabolism and in the urea and tricarboxylic acid cycles. The 2 isoenzymes are homodimeric. In liver about 80% of the enzyme activity is mitochondrial in origin, whereas in serum the enzyme activity is largely cytosolic. Although the mitochondrial and soluble forms of GOT are coded by different chromosomes, the 2 show close homology in amino acid sequence and were presumably derived from a common ancestral gene.
Serum GOT (with SGPT) levels are usually elevated in states of hepatocellular injury (injury to the liver cells), the highest levels are associated with hepatitis of a viral origin. High levels are also found after myocardial infarction, when SGPT levels are lower.

General readings

Teichberg VI.
From the liver to the brain across the blood-brain barrier.
Proc Natl Acad Sci U S A. 2007 May 1;104(18):7315-6. Epub 2007 Apr 26. No
abstract available.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GOT1 (22-35) antibody

  Goat Polyclonal Antibody against GOT1  
TA303294 (0.5µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GOT1 (157-167) antibody

  Goat Polyclonal Antibody against GOT1 (Internal region)  
TA303313 (0.01µg/ml) staining of Human Liver lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu, Rt P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GOT1 antibody

  Aspartate Aminotransferase Goat Polyclonal (aa157-167) Antibody  
Anti-GOT1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. Goat IgG Aff - Purified Hu, Rt E, P, WB
50 µg / €400.00
  OriGene Technologies, Inc.

GOT1 antibody

  Rabbit anti-GOT1 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using GOT1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

GOT1 antibody

  Rabbit Polyclonal Anti-GOT1 Antibody  
Gel: 15%SDS-PAGE, Lysate: 40 ug, Lane 1-3: Human normal liver tissue, mouse brain tissue, Mouse heart tissue, Primary antibody: (GOT1 Antibody) at dilution 1/1600, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 seconds Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GOT1 (N-term) antibody

Western blot analysis of GOT1 Antibody (N-term) (AP18039PU-N) in mouse brain tissue lysates (35ug/lane). GOT1 (arrow) was detected using the purified Pab. Rabbit Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

GOT1 antibody

GOT1 Rabbit IgG Azide Free Por Dot, E, ICC/IF, ID, IP, R, WB
10 mg / €240.00
  Acris Antibodies GmbH

GOT1 antibody

GOT1 Rabbit IgG Biotin Por Dot, E, ICC/IF, ID, IP, R, WB
1 ml / €350.00
  Acris Antibodies GmbH

GOT1 antibody

GOT1 Rabbit IgG Aff - Purified Por Dot, E, ICC/IF, ID, IP, R, WB
1 mg / €440.00
  Acris Antibodies GmbH

GOT1 antibody

GOT1 Sheep Serum Por E, IP, WB
2 ml / €1,180.00
  Acris Antibodies GmbH

GOT1 antibody

GOT1 Sheep IgG Biotin Por E, WB
0.1 mg / €450.00
  Acris Antibodies GmbH

GOT1 antibody

GOT1 Sheep IgG HRP Por E, WB
0.1 mg / €450.00
  Acris Antibodies GmbH

GOT1 antibody

GOT1 Sheep Purified Por E, WB
0.1 mg / €450.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

Got1 - mouse gene knockout kit via CRISPR

Got1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Got1 - mouse gene knockout kit via CRISPR

Got1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Got1 - mouse gene knockout kit via CRISPR

Got1 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Got1 - mouse gene knockout kit via CRISPR

Got1 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

GOT1

  Recombinant protein of human glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) (GOT1)  
GOT1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

GOT1

  Recombinant protein of human glutamic-oxaloacetic transaminase 1, soluble (aspartate aminotransferase 1) (GOT1)  
GOT1 Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
10 µg / €299.00
  OriGene Technologies, Inc.

GOT1 (1-413, His-tag)

Recombinant human GOT1, 1-413aa, His-tagged Human Purified > 95 % by SDS - PAGE E. coli
0.5 mg / €670.00
  Acris Antibodies GmbH

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