GOT2 antibody

Principal name

GOT2 antibody

Alternative names for GOT2 antibody

Aspartate aminotransferase, mitochondrial, mAspAT, Transaminase A, FABP-1, FABPpm, Fatty acid-binding protein

SwissProt ID

P00505 (Human), P00506 (Pig), P00507 (Rat), P05202 (Mouse)

Gene ID

2806 (GOT2), 396968

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Por (Porcine), Bov (Bovine), Can (Canine), Ze (Zebrafish), Chk (Chicken), African clawed frog, Mky (Monkey), Xen (Xenopus), Eq (Equine), GP (Guinea Pig), Rb (Rabbit), Ye (Yeast)

Available hosts

Mouse, Sheep, Goat, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Frozen Sections (C), Dot blot (Dot), Immunoelectron Microscopy (EM), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), Flow Cytometry (F)

Background of GOT2 antibody

Aspartate Aminotransferase [Glutamate oxaloacetate transaminase] is a ubiquitous pyridoxal phosphate-dependent enzyme which exists in both mitochondrial and cytosolic forms. The enzyme plays an important role in amino acid metabolism and in the urea and tricarboxylic acid cycles. The 2 isoenzymes are homodimeric. In liver about 80% of the enzyme activity is mitochondrial in origin, whereas in serum the enzyme activity is largely cytosolic. Although the mitochondrial and soluble forms of GOT are coded by different chromosomes, the 2 show close homology in amino acid sequence and were presumably derived from a common ancestral gene.
Serum GOT [with SGPT] levels are usually elevated in states of hepatocellular injury (injury to the liver cells), the highest levels are associated with hepatitis of a viral origin. High levels are also found after myocardial infarction, when SGPT levels are lower.

General readings

Teichberg VI.
From the liver to the brain across the blood-brain barrier.
Proc Natl Acad Sci U S A. 2007 May 1;104(18):7315-6.
PMID: 17463093

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GOT2 antibody

GOT2 polyclonal antibody (A01), Lot # FHC0061120QCS1. Western Blot analysis of GOT2 expression in NIH/3T3. Mouse Serum Hu, Ms, Rt E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

GOT2 antibody

TA303304 (0.3µg/ml) staining of Human Liver cell lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GOT2 antibody

TA303325 (0.03µg/ml) staining of Human Kidney lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GOT2 antibody

Western blot analysis of lysates from Hela,  HepG2,  HL-60,  Ramos,  mouse brain cell line (from left to right),  using GOT2 Antibody (N-term)(Cat.  #TA325088).  TA325088 was diluted at 1:1000 at each lane.  A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.  Lysates at 35ug per lane. Rabbit IgG Purified Bov, Chk, Hu, Mky, Ms, Por, Rt F, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

GOT2 antibody

Anti-GOT2 antibody (0.3µg/ml) staining of HepG2 lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GOT2 antibody

WB Suggested Anti-GOT2 Antibody; Titration: 1 ug/ml; Positive Control: 293T Whole Cell Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ye, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

GOT2 antibody

WB Suggested Anti-GOT2 Antibody Titration: 1.0ug/ml; ELISA Titer: 1: 312500; Positive Control: 293T cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt, Ye, Ze WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GOT2 antibody

Immunofluorescence analysis of PC-3 (left) and SK-BR-3 (right) cells using anti-GOT2 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye. Mouse IgG1 3E9 Ascites Hu, Mky, Ms, Rt ICC/IF, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

GOT2 antibody

Skeletal Muscle: Formalin-Fixed Paraffin-Embedded (FFPE) Sheep Purified Hu, Por ICC/IF, IP, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

GOT2 (295-306) antibody

AP08818PU-N staining GOT2 in paraffin-embedded Human Liver by Immunohistochemistry. Goat IgG Aff - Purified Bear, Bov, Chk, Eq, Fi, Hst, Hu, Mky, Ms, Op, Pl, Por, Prim, Rt, Tr E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

GOT2 antibody

GOT2 monoclonal antibody (M09), clone 4H8. Western Blot analysis of GOT2 expression in HepG2(Cat # L019V1 ). Mouse IgG2b 4H8 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

GOT2 antibody

GOT2 polyclonal antibody ( Cat # PAB7440 ) ( 0.03 µg/ml ) staining of human kidney lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

GOT2 antibody

GOT2 polyclonal antibody ( Cat # PAB7559 ) ( 0.3 µg/ml ) staining of human liver lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

GOT2 antibody

Human 293T; WB Suggested Anti-GOT2 Antibody. . Titration: 1 ug/ml. . Positive Control: 293T Whole Cell; GOT2 antibody - N-terminal region (ARP43517_P050) in Human 293T cells using Western Blot Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

GOT2 antibody

Human 293T; WB Suggested Anti-GOT2 Antibody Titration: 1.0ug/ml. ELISA Titer: 1:312500. Positive Control: 293T cell lysate; GOT2 antibody - N-terminal region (ARP43517_T100) in Human 293T cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Por, Rt, Ze WB
0.1 mg / €310.00
  AVIVA Systems Biology

GOT2 antibody

Human Liver; GOT2 antibody - C-terminal region (ARP43518_T100) in Human Liver cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Chk, Hu, Ms, Por, Rt, Ze P, WB
0.1 mg / €310.00
  AVIVA Systems Biology

GOT2 antibody

Nb100-93404 (0.03ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemilumines Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

GOT2 antibody

Immunofluorescence: GOT2 Antibody (3E9) [NBP1-47469] - Immunofluorescence analysis of PC-3 (left) and SK-BR-3 (right) cells using anti-GOT2 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye. Mouse IgG1 3E9 Purified Hu, Mky, Ms, Rt E, ICC/IF, WB
0.1 ml / €420.00
  Novus Biologicals Inc.

GOT2 antibody

GOT2 Rabbit Purified Bov, Chk, Hu, Ms, Por, Rt, Xen, Ze P, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

GOT2 (30-430, His-tag)

GOT2 Human Purified > 95 % by SDS - PAGE E. coli
0.5 mg / €730.00
  Acris Antibodies GmbH

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