GPR3 / ACCA antibody

Principal name

GPR3 / ACCA antibody

Alternative names for GPR3 / ACCA antibody

G-protein coupled receptor 3, ACCA orphan receptor

SwissProt ID

P35413 (Mouse), P46089 (Human)

Gene ID

2827 (GPR3), 14748 (Gpr3)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Eq (Equine), Mky (Monkey), Por (Porcine), Rb (Rabbit), Bear, Hst (Hamster), Can (Canine), Bt (Bat)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Dot blot (Dot), Frozen Sections (C)

Background of GPR3 / ACCA antibody

Accumulation of beta amyloid beta peptide (AbP) is a central pathological characteristic of Alzheimer diseased (AD) brain. The APP is generated bt sequential cleavages of the beta amyloid precursor protein (APP) by alfa, beta and gamma secretases (1). The action of alfa secretase with in the AbP generates the AbP sequences and all three secretases are considered to be relevant to therapeutic target for AD. The high throughput functional genomics screening utilizing fullength human FLeXSelect cDNA library composed of 4217 individual adenoviruses representing the transcript of 1905 unique genes encoding potential drug targets was carried out to identify modulators of ABP production (1). Several primary hits affecting AbP were identified including those which are known previously to affect AbP generation like HTR2C, PTGER2 led to an increase in (AbP1-42) production (2) while BACE2 repressed Abp production (3). After application of several prioritizing criteria as secondary assays, including secreted alkaline phosphataase (SEAP) assay, RNA interference assay, real-time PCR, and mass spectrometric analyses of immunoprecipitated AbP one candidate was identified as GPCR3. The GPCR3 gene is mapped to the candidate AD linkage region on chromosome 1p36.1-p34.3. GPCR3 is predominantly expressed din CNS (4). Induction of GPCR3 in human neuroblastoma SHSY-5Y or human embryonic kidney (HEK293) cells led to a robust increase in AbP1-40 and AbP1-42 which is independent of beta secretase expression (1). Mechanistically GPCR3 increases AbP production by increasing the activities of gamma secretases subunits.
GPCR3 is an orphan receptor, but sphingosine and its metabolites (SPC and S1P) are supposedly act as putative ligands and activate the receptor. Expression of GPR3 along with GPR12 and Edg3this preceptor in xenopus oocytes control the cytosolic levels of cAMP and down regulation of GPR3 caused a meiotic resumption in mouse and rat oocyte (3). Incubation of mouse oocytes with the GPR3/12 ligands SPC and S1P delayed spontaneous oocyte maturation, suggesting that basal cAMP levels are maintained by GPR3 and GPR12 receptors in mouse/rat oocytes. GPCR3 is a 7TMD receptor with an apparent molecular weight of 45kDa. There are putative glycosylation sites on the amino terminal end of protein and the third large intra cytoplasmic loop may be the site of protein-protein interaction as evident in many other GPCRs. The receptor is expressed in CNS, gonads and some other organs. Gene bank has at least three variants of rat GPCR3 receptor.

General readings

Tanaka S, Ishii K, Kasai K, Yoon SO, Saeki Y.
Neural expression of G protein-coupled receptors GPR3, GPR6, and GPR12 upregulates cyclic AMP levels and promotes neurite outgrowth.
J Biol Chem. 2007 Apr;282(14):10506-15. Epub 2007 Feb 6.
PMID: 17284443

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GPR3 / ACCA antibody

Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 3B4-G3 Hu E, P, RNAi, WB
50 µg / €530.00
  Acris Antibodies GmbH

GPR3 / ACCA antibody

  Goat Polyclonal Antibody against GPR3  
TA303266 (0.3µg/ml) staining of Human Brain (Frontal Cortex) lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GPR3 / ACCA antibody

  Rabbit Polyclonal GPR3 Antibody  
Western blot analysis of GPR3 in EL4 cell lysate with GPR3 antibody at (A) 1 and (B) 2 ug/ml. Rabbit IgG Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GPR3 / ACCA (1st extracel. dom.) antibody

  GPR3 Rabbit Polyclonal (Extracellular Domain) Antibody  
GPR3 / ACCA Rabbit Aff - Purified Bear, Bov, Eq, Hu, Mky, Ms, Por, Rb, Rt P
50 µg / €400.00
  OriGene Technologies, Inc.

GPR3 / ACCA (2nd extracell. dom.) antibody

  GPR3 Rabbit Polyclonal (Extracellular Domain) Antibody  
GPR3 / ACCA Rabbit Aff - Purified Hst, Hu, Mky, Ms, Rb, Rt P
50 µg / €400.00
  OriGene Technologies, Inc.

GPR3 / ACCA (3rd extracell. dom.) antibody

  GPR3 Rabbit Polyclonal (Extracellular Domain) Antibody  
GPR3 / ACCA Rabbit Aff - Purified Bear, Bov, Can, Elephant, Eq, Hst, Hu, Mky, Ms, Por, Rt E, P
50 µg / €400.00
  OriGene Technologies, Inc.

GPR3 / ACCA (extracell. dom.) (N-term) antibody

  GPR3 Rabbit Polyclonal (N-Terminus) Antibody  
GPR3 / ACCA Rabbit Aff - Purified Bear, Bt, Can, Hu P
50 µg / €400.00
  OriGene Technologies, Inc.

GPR3 / ACCA antibody

GPR3 / ACCA Rabbit IgG Aff - Purified Hu E, WB
0.1 mg / €490.00
  Acris Antibodies GmbH

GPR3 / ACCA (C-term) antibody

Human Small Intestine (formalin-fixed, paraffin-embedded) stained with TRIM5 antibody AP22840PU-N at 2.5 µg/ml followed by biotinylated goat anti-rabbit IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

GPR3 / ACCA antibody

GPR3 staining of Human Brain tissue (paraffin-embedded). Rabbit IgG Aff - Purified Hu P
50 µg / €460.00
  Neuromics Antibodies

CRISPR

Catalog No. Species Donor Vector  

GPR3 - human gene knockout kit via CRISPR

GPR3 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

GPR3 - human gene knockout kit via CRISPR

GPR3 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

GPR3 - human gene knockout kit via CRISPR

GPR3 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

GPR3 - human gene knockout kit via CRISPR

GPR3 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Gpr3 - mouse gene knockout kit via CRISPR

Gpr3 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, GPR3 (mGFP-tagged) - Human G protein-coupled receptor 3 (GPR3), 200 uL, >10^7 TU/mL

Lenti ORF particles, GPR3 (mGFP-tagged) - Human G protein-coupled receptor 3 (GPR3), 200 uL, >10^7 TU/mL  
200 µl / €960.00
  OriGene Technologies, Inc.

Lenti ORF particles, GPR3 (Myc-DDK tagged) - Human G protein-coupled receptor 3 (GPR3) , 200 uL, >10^7 TU/mL

Lenti ORF particles, GPR3 (Myc-DDK tagged) - Human G protein-coupled receptor 3 (GPR3) , 200 uL, >10^7 TU/mL  
200 µl / €860.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Gpr3 (GFP-tagged) - Mouse G-protein coupled receptor 3 (Gpr3), 200 uL, >10^7 TU/mL

Lenti ORF particles, Gpr3 (GFP-tagged) - Mouse G-protein coupled receptor 3 (Gpr3), 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, Gpr3 (Myc-DDK-tagged) - Mouse G-protein coupled receptor 3 (Gpr3), 200 uL, >10^7 TU/mL

Lenti ORF particles, Gpr3 (Myc-DDK-tagged) - Mouse G-protein coupled receptor 3 (Gpr3), 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

19 Item(s)

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