GPR75 antibody

Principal name

GPR75 antibody

Alternative names for GPR75 antibody

Orphan retinal GPCR, G-protein coupled receptor 75

SwissProt ID

O95800 (Human), Q6X632 (Mouse)

Gene ID

10936 (GPR75)

Available reactivities

Chk (Chicken), Eq (Equine), Hu (Human), Ms (Mouse), Op (Opossum), Por (Porcine), Rb (Rabbit), Xen (Xenopus), Ze (Zebrafish), Tr (Turkey), L (Lizard), Bt (Bat), Bov (Bovine), Mky (Monkey), Rt (Rat), Bear, African clawed frog, Hst (Hamster)

Available hosts

Rabbit

Available applications

Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Frozen Sections (C), Immunoprecipitation (IP)

Background of GPR75 antibody

Recently a novel human G-protein coupled receptor gene has been characterized and mapped to chromosome 2p16. This gene codes for a 540 amino acid protein in retinal pigment epithelium (RPE) and cells surrounding retinal arterioles. In contrast, the Northern blot data obtained from mouse sections suggest the expression of transcripts in photoreceptor inner segments and I outer plexiform layer. The transcripts of the GPCR-75 gene (7kb) are also found in abundance in brain sections. So far, no mutations in GPCR-75 protein were identified in patients suffering from Doyne’s honeycomb retinal dystrophy (DHRD), an inherited retinal degeneration disease that maps to chromosome 2p16 (1).
The GPCR-75 protein is approximately 78 kDa (540 amino acids) protein that is primarily expressed in human retinal pigment epithelium (RPEs). The GPCR-75 sequence analyses suggest the presence of 7 trans-membrane domains, a characteristic feature of GPCR. The protein has putative N-glycosylation sites near the extra cellular N-terminal end of the proteins. The protein has a large 3 intra cellular loop which might be the site for interaction of G-proteins. The short carboxy terminal is intracellular and has putative post-translational modification lipid modification sites.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GPR75 antibody

Western blot analysis of extracts from HT-29 cells and K562 cells, using GPR75 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GPR75 antibody

WB Suggested Anti-GPR75 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Eq, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

GPR75 antibody

WB Suggested Anti-GPR75 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 2500; Positive Control: COLO205 cell lysateGPR75 is supported by BioGPS gene expression data to be expressed in COLO205 Rabbit IgG Purified Bov, Eq, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

GPR75 antibody

WB Suggested Anti-GPR75 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:100; Positive Control: Hela cell lysate Rabbit IgG Purified Bov, Eq, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

GPR75 antibody

Anti-GPR75 antibody IHC of human brain, neurons and glia. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Purified Chk, Hu, Ms, Rt, Xen
50 µg / €379.00
  OriGene Technologies, Inc.

GPR75 (C-term) antibody

Anti-GPR75 antibody IHC of human eye, retina. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Chk, Elephant, Eq, Hu, L, Ms, Op, Por, Rb, Tr, Xen, Ze P
50 µg / €379.00
  OriGene Technologies, Inc.

GPR75 (N-term Extracell. Dom.) antibody

Anti-GPR75 antibody IHC of human eye, retina. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Hu, Mky, Prim P
50 µg / €379.00
  OriGene Technologies, Inc.

GPR75 antibody

Anti-GPR75 antibody IHC of human brain, cortex, neurons. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 4-7 ug/ml. Rabbit Purified Bov, Hu, Mky, Por, Rb
50 µg / €379.00
  OriGene Technologies, Inc.

GPR75 antibody

Anti-GPR75 antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3-7 ug/ml. Rabbit Purified Bov, Hst, Hu, Ms, Por, Rt
50 µg / €379.00
  OriGene Technologies, Inc.

GPR75 (3rd cytopl. dom.) antibody

Anti-GPR75 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Rabbit Aff - Purified Bear, Bov, Bt, Elephant, Eq, Hu, Mky, Ms, Por, Rb, Rt, Xen P
50 µg / €379.00
  OriGene Technologies, Inc.

GPR75 antibody

Anti-GPR75 antibody IHC of human brain, cortex, neurons. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10-25 ug/ml. Rabbit Purified Hst, Hu, Ms, Rt
50 µg / €379.00
  OriGene Technologies, Inc.

GPR75 (2nd Cytopl. Dom.) antibody

Anti-GPR75 antibody IHC of human brain, cortex, neurons. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 18 ug/ml. Rabbit Aff - Purified Bear, Bov, Bt, Chk, Elephant, Eq, Fi, Hst, Hu, L, Mky, Ms, Por, Prim, Rb, Rt, Xen P
50 µg / €379.00
  OriGene Technologies, Inc.

GPR75 Control Peptide

GPR75
0.25 mg / €250.00
  Acris Antibodies GmbH

GPR75 antibody

GPR75 Rabbit Aff - Purified Hu, Ms, Rt C, E, IP, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

GPR75 antibody

Immunofluorescence analysis of LOVO cells, using GPR75 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, WB
50 µg / €205.00
  Assay Biotech

GPR75 antibody

Immunofluorescence analysis of LOVO cells, using GPR75 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, ICC/IF, WB
0.1 mg / €310.00
  Assay Biotech

GPR75 antibody

Hum. Adult Placenta; Host:Rabbit. Target Name:GPR75. Sample Tissue:Human Adult Placenta. Antibody Dilution: 1.0ug/ml.; GPR75 antibody - middle region (ARP33103_P050) in Hum. Adult Placenta cells using Western Blot Rabbit Aff - Purified African clawed frog, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

GPR75 antibody

Human COLO205; WB Suggested Anti-GPR75 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:2500. Positive Control: COLO205 cell lysate. GPR75 is supported by BioGPS gene expression data to be expressed in COLO205.; GPR75 antibody - middle region (ARP33104_P050) in Human COLO205 cells using Western Blot Rabbit Aff - Purified Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

GPR75 (C-term) antibody

Human HeLa; WB Suggested Anti-GPR75 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:100. Positive Control: Hela cell lysate; GPR75 antibody - C-terminal region (ARP34088_P050) in Human HeLa cells using Western Blot Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

GPR75 antibody

Immunofluorescent analysis of GPR75 staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4  C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

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