Granzyme B (GZMB) antibody

Principal name

Granzyme B (GZMB) antibody

Alternative names for Granzyme B (GZMB) antibody

Granzyme-2, CGL1, CTSGL1, CSPB, CTLA1, CTLA-1, GRB, SECT, Fragmentin-2, T-cell serine protease 1-3E, Cytotoxic T-lymphocyte proteinase 2, Lymphocyte protease, Cathepsin G-like 1

SwissProt ID

P04187 (Mouse), P10144 (Human)

Gene ID

3002 (GZMB), 14939 (Gzmb)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Por (Porcine), Chimp (Chimpanzee), Mky (Monkey), Bov (Bovine)

Available hosts

Mouse, Rabbit, Rat

Available applications

Frozen Sections (C), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Flow Cytometry (F), Paraffin Sections (P), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of Granzyme B (GZMB) antibody

Granzyme B is a serine protease expressed by cytotoxic T cells (CTL) and NK cells. Its main function is to induce cell death to eliminate harmful targets such as allogeneic, virally infected, and tumor cells. This is evident by the fact that CTLs from mice deficient of granzyme B exhibit a profound defect in inducing rapid DNA fragmentation and apoptosis in target cells. Following receptor-mediated conjugate formation between CTL or NK and their target cell, granzyme B enters the target via endocytosis, and subsequently activates multiple protein substrates to induce apoptosis. Most circulating CD56+ and CD8- NK cells, and approximately half of circulating CD8+ T cells, coexpress both granzyme A and B. In contrast, few circulating CD4+ T cells express granzymes A or B. Activation of CD8+ and CD4+ T cells induces substantial expression of granzyme B, but not granzyme A. Besides CTL and NK, evidence has shown that the distribution of human granzyme B has a broader spectrum of cells including CD34+ hematopoietic progenitor cells, keratinocytes, basophils, mast cells, plasmacytoid dendritic cells, and B cells. Although its role in cytotoxic lymphocyte-mediated apoptosis is well established, granzyme B can also degrade extracellular matrix proteins and alter inflammation, if present in the extracellular milieu. These findings suggest that granzyme B can function as an activation molecule with potentially important immunoregulatory functions. In addition, it was shown that expression of granzyme B is elevated in acute coronary syndrome and acute myocardial infarction, indicating that granzyme B could be a factor involved in cardiovascular diseases.

General readings

1. Mohamedali KA  et al. The functionalized human serine protease granzyme B/VEGF121 targets tumor vasculature and ablates tumor growth. Mol Cancer Ther 12:2055-66 (2013). ICC/IF .
2. Chaitanya GV  et al. Granzyme-b is involved in mediating post-ischemic neuronal death during focal cerebral ischemia in rat model. Neuroscience 165:1203-16 (2010). WB ; Rat .

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Granzyme B (GZMB) antibody

Immunohistochemical staining of paraffin-embedded Human Kidney tissue within the normal limits using anti-GZMB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505930) Mouse IgG2a OTIE6 Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Granzyme B (GZMB) antibody

Immunohistochemical staining of paraffin-embedded Human lymphoma tissue using anti-GZMB mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 120C for 3min, TA505931) Mouse IgG1 OTI4E4 Purified Hu ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Granzyme B (GZMB) antibody

Human tonsil stained with anti-Granzyme B antibody Rabbit Purified Can, Hu, Ms, Por, Rt P
0.5 ml / €360.00
  Acris Antibodies GmbH

Granzyme B (GZMB) antibody

Human tonsil stained with anti-Granzyme B antibody Rabbit Purified Can, Hu, Ms, Por, Rt P
1 ml / €490.00
  Acris Antibodies GmbH

Granzyme B (GZMB) antibody

Human tonsil stained with anti-Granzyme B antibody Rabbit Purified Can, Hu, Ms, Por, Rt P
0.1 ml / €200.00
  Acris Antibodies GmbH

Granzyme B (GZMB) antibody

Western blot analysis of extracts from NIH/3T3 cells, using Granzyme B antibody (#TA312131).The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Granzyme B (GZMB) antibody

Predicted cell location: Cytoplasm. Positive control: Human colon cancer tissue. Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded human colon cancer tissue using GZMB antibody at dilution 1/30, on the right is treated with the fusion protein. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Granzyme B (GZMB) antibody

Western blot analysis of extracts of various cell lines, using GZMB antibody. Rabbit IgG Purified Hu, Ms
0.1 mg / €325.00
  OriGene Technologies, Inc.

Granzyme B (GZMB) antibody

Western blot analysis on NIH-3T3 cell lysate using Granzyme B Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Granzyme B (GZMB) antibody

Western blot analysis of PBMC (lane 1), PBMC activated with 5 ug/mL CD3 antibody coated on the plate and 2 ug/mL CD28 antibody for two days (lane 2) using anti-Granzyme B antibody (clone M3304B06).  Anti-beta; actin antibody (poly6221) was used as a loading control. Mouse IgG1 M3304B06 Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Granzyme B (GZMB) antibody

5 ng of recombinant, His-thioredoxin tagged mouse Granzyme B was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-mouse Granzyme B (clone 12F9B65) antibody. Proteins were visualized using an anti-rat-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Rat 12F9B65 Purified Ms WB
0.1 mg / €248.00
  OriGene Technologies, Inc.

Granzyme B (GZMB) antibody

Human Tonsil stained with anti-Granzyme B antibody. Rabbit IgG Purified Hu P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Granzyme B (GZMB) antibody

Granzyme B (GZMB) Mouse IgG1 B18.1 Aff - Purified Hu C, F, IP, P, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

Granzyme B (GZMB) antibody

Granzyme B (GZMB) Mouse IgG2a GrB-7 Supernatant Hu P, WB
5 ml / €1,710.00
  Acris Antibodies GmbH

Granzyme B (GZMB) antibody

Granzyme B (GZMB) Mouse IgG2a GrB-7 Supernatant Hu P, WB
1 ml / €420.00
  Acris Antibodies GmbH

Granzyme B (GZMB) antibody

Immunohistochemistry (IHC) analyzes of Granzyme B/H antibody (Cat.-No.: AP06147PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Granzyme B (GZMB) (N-term) antibody

Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu, Ms, Rt P
50 µl / €470.00
  Acris Antibodies GmbH

Granzyme B (GZMB) (C-term) antibody

Western blot analysis of GZMB Antibody (C-term) (AP17448PU-N) in HL-60 cell line lysates (35 µg/lane). GZMB (arrow) was detected using the purified Pab. Rabbit Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Granzyme B (GZMB) (N-term) antibody

Western blot analysis of GZMB antibody (N-term) (AP17449PU-N) in mouse stomach lysates (35 µg/lane). GZMB (arrow) was detected using the purified Pab. Rabbit Hu, Ms WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Granzyme B (GZMB) antibody

Figure.3: BOSC cells were transiently transfected with expression vectors for Granzyme A, B, K, or M. Expression of the constructs was tested with an anti-tag monoclonal antibody (green curves), an irrelevant monoclonal antibody served as negative control (black curves). For specificity testing, purified Granzyme B antibody was tested on all transfectants. A positive signal wasobtained only with Granzyme B transfected cells (red curves). Mouse IgG1 GM4C1 Purified Hu E, F
0.1 mg / €360.00
  Acris Antibodies GmbH

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