GRAP antibody

Principal name

GRAP antibody

Alternative names for GRAP antibody

GRB2-related adapter protein

SwissProt ID

A6QLK6 (Bovin), Q13588 (Human), Q9CX99 (Mouse)

Gene ID

10750 (GRAP)

Available reactivities

Hu (Human), Bov (Bovine), Ms (Mouse), Rt (Rat), Mky (Monkey), Can (Canine), Eq (Equine), GP (Guinea Pig), Gt (Goat), Por (Porcine), Rb (Rabbit), Ze (Zebrafish)

Available hosts

Mouse, Goat, Rabbit

Available applications

ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of GRAP antibody

This gene encodes a member of the GRB2/Sem5/Drk family and functions as a cytoplasmic sigling protein which contains an SH2 domain flanked by two SH3 domains. The SH2 domain interacts with ligand-activated receptors for stem cell factor and erythropoietin, and facilitates the formation of a stable complex with the BCR-ABL oncoprotein. This protein also associates with the Ras guanine nucleotide exchange factor SOS1 (son of sevenless homolog 1) through its N-termil SH3 domain. In general, it couples sigls from receptor and cytoplasmic tyrosine kises to the Ras sigling pathway.

General readings

Feng GS, Ouyang YB, Hu DP, Shi ZQ, Gentz R, Ni J.
Grap is a novel SH3-SH2-SH3 adaptor protein that couples tyrosine kinases to the Ras pathway.
J Biol Chem 1996 May 24;271(21):12129-32
PMID: 8647802

16 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GRAP (C-term) antibody

TA302717 (0.3µg/ml) staining of human thymus lysate (35µg protein in RIPA buffer).  Primary incubation was 1 hour.  Detected by chemiluminescence. Goat Aff - Purified Bov, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GRAP antibody

Sample (30 ug of whole cell lysate). A: A549 12% SDS PAGE. TA308618 diluted at 1:1000. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GRAP antibody

Host: Rabbit; Target Name: GRAP; Sample Tissue: Fetal Brain lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Gt, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

GRAP antibody

WB Suggested Anti-GRAP Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 62500; Positive Control: THP-1 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

GRAP antibody

Western Blot analysis of GRAP expression in transfected 293T cell line (H00010750-T01) by GRAP MaxPab polyclonal antibody.

Lane 1: GRAP transfected lysate(23.87 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

GRAP antibody

GRAP polyclonal antibody ( Cat # PAB6031 ) staining ( 0.3 µg/ml ) of human thymus lysate ( 35 µg protein in RIPA buffer ) . Primary incubation was 1 hour. Detected by chemiluminescence. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

GRAP antibody

Western blot analysis of extracts from 293/MCF-7/COLO cells, using GRAP Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

GRAP antibody

Western blot analysis of extracts from 293/MCF-7/COLO cells, using GRAP Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

GRAP antibody

GRAP Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

GRAP antibody

NB100-800 (0.3ug/ml) staining of Human Thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

GRAP antibody

Western  Blot:  GRAP Antibody [NBP1-33626] – Western blot analysis of GRAP on A549 lysates using NBP1-33626 at a dilution of 1:1000. 30ug whole cell lysate loaded on 12% SDS PAGE gel. Rabbit IgG Hu ICC/IF, P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

GRAP

GRAP Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

GRAP

GRAP Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

GRAP

GRAP Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

GRAP 293T Cell Transient Overexpression Lysate(Denatured)

GRAP 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

GRAP overexpression lysate

GRAP overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

16 Item(s)

per page
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