GRAP2 antibody

Principal name

GRAP2 antibody

Alternative names for GRAP2 antibody

GADS, GRBLG, GRB2L, Grf-40, GRBX, GRID, GRB2-related adapter protein 2, Growth factor receptor-binding protein, GRB-2-like protein, Grf40 adapter protein, Hematopoietic cell-associated adapter protein GrpL, Adapter protein GRID, SH3-SH2-SH3 adapter Mona

SwissProt ID

O75791 (Human), O89100 (Mouse)

Gene ID

9402 (GRAP2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Por (Porcine), Bov (Bovine), Can (Canine)

Available hosts

Rabbit, Mouse, Goat

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Flow Cytometry (F), EPair for Elisa (EPair), ELISA (detection) (E(detection))

Background of GRAP2 antibody

In general, it exists in association with catalytic domains, as in the nonreceptor protein-tyrosine kinases and phospholipase C-γ, within structural proteins, such as spectrin or myosin, and in small adapter proteins, such as Crk and GRB2. SH3 domains are often accompanied by SH2 domains of 100 amino acids that bind to tyrosine-phosphorylated regions of target proteins, frequently linking activated growth factors to putative signal transduction proteins. Deletion or mutation of SH3 domains generally activates the transforming potential of nonreceptor tyrosine kinases, suggesting that SH3 mediates negative regulation of an intrinsic transforming activity. Gads is an adapter proteins that contains both SH2 and SH3 domains. Gads binds to tyrosine-phosphorylated proteins, such as Shc, and functions to couple these proteins to downstream effectors.

General readings

Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010)
Preisinger, C., et al. Cell. Signal. 22(5):848-856 (2010)
Davila, S., et al. Genes Immun. 11(3):232-238 (2010)
Segat, L., et al. J. Gastroenterol. Hepatol. 24(12):1840-1846 (2009)
Ludwig, L., et al. Cancer Lett. 275(2):194-197 (2009)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GRAP2 antibody

Immunohistochemical staining of paraffin-embedded Adenocarcinoma of Human endometrium tissue using anti-GRAP2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA502923) Mouse IgG1 OTI1G2 Purified Hu F, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

GRAP2 antibody

Immunoperoxidase of monoclonal antibody to GRAP2 on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3 ug/ml] Mouse IgG2a 1G12 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

GRAP2 (C-term) antibody

TA302501 staining (0.5µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Hu, Ms, Por WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GRAP2 antibody

Sample (30 ug of whole cell lysate). A: JurKat. 10% SDS PAGE. TA308365 diluted at 1:1000. Rabbit IgG Aff - Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GRAP2 antibody

Western blot analysis of extracts from HuvEc cells, using GRAP2 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GRAP2 antibody

Western blot detection of GRAP2 in GRAP2 positive cell (CEM,Molt-4,Jurkat) and negative cell (3T3,K562,Hela) lysates using GRAP2 mouse mAb (1:1000 diluted).Predicted band size:38KDa.Observed band size:38KDa. Mouse IgG2b 5C7-F4-E7 Purified WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GRAP2 antibody

GRAP2 Mouse IgG2a UW40 Aff - Purified Hu F, IP, WB
0.1 mg / €260.00
  Acris Antibodies GmbH

GRAP2 antibody

Western blot (WB) analysis of Gads antibody (Cat.-No.: AP21183PU-N) in extracts from HUVEC cells. Rabbit Aff - Purified Hu WB
0.1 mg / €350.00
  Acris Antibodies GmbH

GRAP2 (Center) antibody

Western blot analysis of GRAP2 Antibody (Center) Cat.-No AP51943PU-N in MCF-7 cell line lysates (35ug/lane). This demonstrates the GRAP2 antibody detected the GRAP2 protein (arrow). Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

GRAP2 antibody

Western Blot analysis of GRAP2 expression in transfected 293T cell line (H00009402-T02) by GRAP2 MaxPab polyclonal antibody.

Lane 1: GRAP2 transfected lysate(36.3 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

GRAP2 antibody

GRAP2 Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

GRAP2 antibody

Western Blot analysis of GRAP2 expression in transfected 293T cell line (H00009402-T01) by GRAP2 MaxPab polyclonal antibody.

Lane 1: GRAP2 transfected lysate(36.3 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

GRAP2 antibody

GRAP2 Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

GRAP2 antibody

GRAP2 MaxPab rabbit polyclonal antibody. Western Blot analysis of GRAP2 expression in Jurkat. Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

GRAP2 antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between GRAP2 and CSF1R. HeLa cells were stained with anti-GRAP2 rabbit purified polyclonal 1:1200 and anti-CSF1R mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

GRAP2 antibody

HEK293 overexpressing GRAP2 and probed with GRAP2 polyclonal antibody ( Cat # PAB6036 ) ( mock transfection in first lane ), tested by Origene. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

GRAP2 antibody

Western blot analysis of extracts from HuvEc cells, using GRAP2 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
50 µg / €205.00
  Assay Biotech

GRAP2 antibody

Western blot analysis of extracts from HuvEc cells, using GRAP2 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, WB
0.1 mg / €310.00
  Assay Biotech

GRAP2 antibody

Western Blot: GRAP2 Antibody [NB100-805] - staining (0.5ug/ml) of Jurkat lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Goat IgG Aff - Purified Ms, Rt E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

GRAP2 antibody

GRAP2 Rabbit Aff - Purified Hu WB
0.1 mg / €420.00
  Novus Biologicals Inc.

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