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GRB10 / GRBIR antibody

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Principal name

GRB10 / GRBIR antibody

Alternative names for GRB10 / GRBIR antibody

Grb-IR, KIAA0207, Growth factor receptor-bound protein 10, Insulin receptor-binding protein Grb-IR

SwissProt ID

P0CE43 (Rat), Q13322 (Human), Q60760 (Mouse), Q92907 (Human)

Gene ID

2887 (GRB10), 14783 (Grb10)

Ncbi ID

9606, NP_001001549, NP_001001549.1, NP_005302, NM_005311, NM_001001549, NM_001001550, NP_001001555, NP_001001550, NP_001171100.1, NM_001177629, NM_010345

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of GRB10 / GRBIR antibody

Many growth factors function by binding receptors with intrinsic tyrosine kinase activity. Signaling by such receptors involves a series of intermediates characterized by SH2 domains that bind tyrosine phosphorylated receptors by a direct interaction between the SH2 domain and specific phosphotyrosine- containing receptor sequences. GRB7, a SH2 domain protein, has a single SH2 domain at its C-terminal, a central region with similarity to Ras GAP, and a proline-rich N terminus. A related SH2 domain-containing protein, GRB10, exhibits a high degree of homology with GRB7. GRB10 undergoes serine but not tyrosine phosphorylation in response to EGF treatment, but appears to bind to the EGF receptor poorly. GRB10 maps to mouse chromosome 11, in close proximity to the EGF receptor. Similarly, GRB7 maps to the same mouse chromosome near the EGF receptor-related protein HER2.

General readings

Liu F and Roth RA. Grb-IR: a SH2-domain-containing protein that binds to the insulin receptor and inhibits its function. Proc. Natl. Acad. Sci. USA 1995; 92:10287-91.

H00002887-M01 - GRB10 / GRBIR
Detection limit for recombinant GST tagged GRB10 is approximately 0.03ng/ml as a capture antibody.
H00002887-D01P - GRB10 / GRBIR
Western Blot analysis of GRB10 expression in transfected 293T cell line (H00002887-T04) by GRB10 MaxPab polyclonal antibody.Lane 1: GRB10 transfected lysate(60.80 KDa).Lane 2: Non-transfected lysate.
AP20303PU-N - GRB10 / GRBIR
Immunohistochemistry analyzes of GRB10 antibody (Cat.-No.: AP20303PU-N) in paraffin-embedded human brain tissue.
AP20303PU-N - GRB10 / GRBIR
Western blot analysis of GRB10 antibody (Cat.-No.: AP20303PU-N) at 1/500 dilution.
Lane 1: HEK293T whole cell lysate. 
Lane 2: Raw364.7 whole cell lysate. 
Lane 2: PC12 whole cell lysate.
AP20303PU-N - GRB10 / GRBIR
Western blot analysis of GRB10 antibody (Cat.-No.: AP20303PU-N) in extracts from NIH/3T3 cells.
A1031-1 - GRB10 / GRBIR
Immunofluorescence analysis of HepG2 cells, using GRB10 (Phospho-Tyr67) Antibody. The picture on the right is treated with the synthesized peptide.
A1031-1 - GRB10 / GRBIR
Western blot analysis of extracts from NIH-3T3 cells treated with Insulin 0.01U/ml 15', using GRB10 (Phospho-Tyr67) Antibody. The lane on the right is treated with the synthesized peptide.
B1031-1 - GRB10 / GRBIR
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using GRB10 (Ab-67) Antibody. The picture on the right is treated with the synthesized peptide.
B1031-1 - GRB10 / GRBIR
Immunofluorescence analysis of HepG2 cells, using GRB10 (Ab-67) Antibody. The picture on the right is treated with the synthesized peptide.
B1031-1 - GRB10 / GRBIR
Western blot analysis of extracts from NIH-3T3 cells, treated with Insulin 0.01U/ml 15', using GRB10 (Ab-67) Antibody. The lane on the right is treated with the synthesized peptide.
AP32960PU-N - GRB10 / GRBIR
AP32960PU-N(1µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
CPA1498-100ul - GRB10 / GRBIR
Immunofluorescent analysis of GRB10 (pY67) staining in PC12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
CPA1498-100ul - GRB10 / GRBIR
Western blot analysis of GRB10 (pY67) expression in HEK293T insulin-treated (A); mouse spleen (B); PC12 insulin-treated (C) whole cell lysates.
TP306853 - GRB10 / GRBIR
TP320822 - GRB10 / GRBIR
TA319657 - GRB10 / GRBIR
Western blot analysis of GRB10 in SK-N-SH cell lysate with GRB10 antibody at (A) 1 and (B) 2 ug/mL.
TA319657 - GRB10 / GRBIR
Immunofluorescence of GRB10 in rat brain cells with GRB10 antibody at 20 ug/mL.
TA322628 - GRB10 / GRBIR
Predicted cell location: Cytoplasm, Cell membrane. Positive control: Human tonsil tissue. Recommended dilution: 1/10-50 The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using GRB10 antibody at dilution 1/12, on the right is treated with the synthetic peptide. (Original magnification: x200)
TA322629 - GRB10 / GRBIR
Predicted cell location: Cytoplasm, Cell membrane. Positive control: Human cervical cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using GRB10 antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification:x200)
TA325016 - GRB10 / GRBIR
GRB10 Antibody (N-term) (Cat. #TA325016) western blot analysis in A549,MDA-MB453,U251 cell line lysates (35ug/lane).This demonstrates the GRB10 antibody detected the GRB10 protein (arrow).
TA313962 - GRB10
Western blot analysis of extracts from NIH/3T3 cells, treated with Insulin (0.01U/ml, 15mins), using GRB10 (Phospho-Tyr67) antibody.The lane on the right is treated with the synthesized peptide.
TA313962 - GRB10
Immunofluorescence analysis of HepG2 cells, using GRB10 (Phospho-Tyr67) antibody.The picture on the right is treated with the synthesized peptide.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GRB10 / GRBIR antibody
AP32960PU-N(1µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu WB
0.1 mg / €350.00
  Acris Antibodies GmbH
 

Acris recommends this product for Western blotting.

GRB10 / GRBIR antibody
  Rabbit polyclonal GRB10 (Tyr67) antibody(Phospho-specific)  
Western blot analysis of extracts from NIH/3T3 cells, treated with Insulin (0.01U/ml, 15mins), using GRB10 (Phospho-Tyr67) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, ICC/IF, WB
0.1 ml / €335.00
  OriGene Technologies, Inc.
+1 additional image

GRB10 / GRBIR (C-term) antibody
  Rabbit Polyclonal GRB10 Antibody  
Western blot analysis of GRB10 in SK-N-SH cell lysate with GRB10 antibody at (A) 1 and (B) 2 ug/mL. Rabbit IgG Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €371.00
  OriGene Technologies, Inc.
+1 additional image

GRB10 / GRBIR antibody
  Anti-GRB10 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm, Cell membrane. Positive control: Human tonsil tissue. Recommended dilution: 1/10-50 The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using GRB10 antibody at dilution 1/12, on the right is treated with the synthetic peptide. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.

GRB10 / GRBIR antibody
  Anti-GRB10 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm, Cell membrane. Positive control: Human cervical cancer tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using GRB10 antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €335.00
  OriGene Technologies, Inc.

GRB10 / GRBIR antibody
  Rabbit polyclonal GRB10 Antibody (N-term)  
GRB10 Antibody (N-term) (Cat. #TA325016) western blot analysis in A549,MDA-MB453,U251 cell line lysates (35ug/lane).This demonstrates the GRB10 antibody detected the GRB10 protein (arrow). Rabbit IgG Purified Hu WB
0.4 ml / €410.00
  OriGene Technologies, Inc.

GRB10 / GRBIR antibody
Immunohistochemistry analyzes of GRB10 antibody (Cat.-No.: AP20303PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH
+2 additional images

GRB10 / GRBIR pTyr67 antibody
Immunofluorescence analysis of HepG2 cells, using GRB10 (Phospho-Tyr67) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, ICC/IF, WB
50 µg / €230.00
  Assay Biotech
+1 additional image

GRB10 / GRBIR pTyr67 antibody
Immunofluorescence analysis of HepG2 cells, using GRB10 (Phospho-Tyr67) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, ICC/IF, WB
0.1 mg / €330.00
  Assay Biotech
+1 additional image

GRB10 / GRBIR antibody
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using GRB10 (Ab-67) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, ICC/IF, P, WB
50 µg / €230.00
  Assay Biotech
+2 additional images

GRB10 / GRBIR antibody
Immunohistochemistry analysis of paraffin-embedded human brain tissue, using GRB10 (Ab-67) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu E, ICC/IF, P, WB
0.1 mg / €330.00
  Assay Biotech
+2 additional images

CRISPR

Catalog No. Species Donor Vector  

GRB10 - human gene knockout kit via CRISPR
GRB10 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

GRB10 - human gene knockout kit via CRISPR
GRB10 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

GRB10 - human gene knockout kit via CRISPR
GRB10 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

GRB10 - human gene knockout kit via CRISPR
GRB10 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Grb10 - mouse gene knockout kit via CRISPR
Grb10 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Grb10 - mouse gene knockout kit via CRISPR
Grb10 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Grb10 - mouse gene knockout kit via CRISPR
Grb10 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Grb10 - mouse gene knockout kit via CRISPR
Grb10 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

GRB10 / GRBIR (transcript variant 4)
  Recombinant protein of human growth factor receptor-bound protein 10 (GRB10), transcript variant 4  
GRB10 / GRBIR Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.		 HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

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