GRB2 / ASH antibody

Principal name

GRB2 / ASH antibody

Alternative names for GRB2 / ASH antibody

Growth factor receptor-bound protein 2

SwissProt ID

P62993 (Human), P62994 (Rat), Q07883 (Chick), Q60631 (Mouse)

Gene ID

2885 (GRB2), 14784 (Grb2), 81504

Available reactivities

Hu (Human), Bov (Bovine), Ms (Mouse), Rt (Rat), Mky (Monkey), Por (Porcine), Ze (Zebrafish), Can (Canine)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of GRB2 / ASH antibody

GRB2, Growth factor receptor-bound protein 2, is an adaptor protein involved in signal transduction/cell communication.The GRB2 gene encodes a protein that has homology to noncatalytic regions of the SRC oncogene product. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. In humans, the GRB2 protein is encoded by the GRB2 gene. Grb2 is widely expressed and is essential for multiple cellular functions. The protein encoded by this gene binds receptors such as the epidermal growth factor receptor and contains one SH2 domain and two SH3 domains. Its two SH3 domains direct complex formation with proline-rich regions of other proteins, and its SH2 domain binds tyrosine phosphorylated sequences. This gene is similar to the sem-5 gene of Caenorhabditis elegans, which is involved in the signal transduction pathway. Two alternatively spliced transcript variants encoding different isoforms have been found for this gene.

General readings

Rudd CE., et al. (2009) Immunol Rev. 229(1):12-26.
Weisswange I., et al. (2009) Nature. 458(7234):87-91.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GRB2 / ASH antibody

  Rabbit Polyclonal Antibody against GRB2 (Y209)  
Western blot analysis of lysate from Ramos cell line,  using GRB2 Antibody (pY209)(Cat.  #TA302011).  TA302011 was diluted at 1:1000.  A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody.  Lysate at 35ug. Rabbit Ig Aff - Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GRB2 / ASH (C-term) antibody

  Goat Polyclonal Antibody against GRB2  
TA302665 staining (2µg/ml) of Jurkat lysate (RIPA buffer, 35µg total protein per lane).  Primary incubated for 1 hour.  Detected by western blot using chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GRB2 / ASH antibody

  Rabbit polyclonal GRB2 (Phospho-Ser159) antibody  
Western blot analysis of extracts from HT-29 cells, treated with serum (20%, 15mins), using GRB2 (Phospho-Ser159) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GRB2 / ASH antibody

  Rabbit anti-GRB2 Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using GRB2 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

GRB2 / ASH antibody

  Mouse Monoclonal GRB2 Antibody  
Western blot detection of GRB2 in K562,Raji,Jurkat,Ramos,C6,3T3 and Hela cell lysates using GRB2 mouse mAb (1:1000 diluted).Predicted band size:25KDa.Observed band size:25KDa. Mouse IgG1 5G3-E7-F11 Purified WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GRB2 / ASH antibody

  Rabbit Polyclonal Anti-GRB2 Antibody  
The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using (GRB2 Antibody) at dilution 1/25, on the right is treated with fusion protein. (Original magnification: ×200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

GRB2 / ASH (N-term) antibody

GRB2  / ASH Mouse IgG1 3F2 Purified Hu, Ms, Rt WB
0.1 ml / €360.00
  Acris Antibodies GmbH

GRB2 / ASH (198-217) antibody

GRB2  / ASH Rabbit IgG Aff - Purified Bov, Hu, Ms, Rt WB
0.1 mg / €260.00
  Acris Antibodies GmbH

GRB2 / ASH antibody

Formalin-fixed and paraffin-embedded human brain tissue reacted with GRB2 antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu P, WB

Regular Price: 0.4 ml / €370.00

Special Price: 0.4 ml / €231.00

  Acris Antibodies GmbH

GRB2 / ASH antibody

Western blot (WB) analysis of GRB2 antibody (Cat.-No.: AP21184PU-N) in extracts from Jurkat cells. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €350.00
  Acris Antibodies GmbH

GRB2 / ASH (C-term) antibody

Immunohistochemistry Image: Human Pancreas: Formalin-Fixed, Paraffin-Embedded (FFPE) Goat Purified Bov, Bt, Can, Eq, Hst, Hu, Mky, Ms, Por, Rb, Rt E, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

GRB2 / ASH antibody

Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Purified Hu P, WB
50 µl / €470.00
  Acris Antibodies GmbH

GRB2 / ASH (C-term) antibody

IHC Image for AP23211PU-N
Human Colon, Myenteric Plexus: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Aff - Purified Hu P, WB
50 µg / €470.00
  Acris Antibodies GmbH

GRB2 / ASH antibody

Western blot analysis of extracts from Jurkat cells, using GRB2 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €230.00
  Assay Biotech

GRB2 / ASH antibody

Western blot analysis of extracts from Jurkat cells, using GRB2 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

GRB2 - human gene knockout kit via CRISPR

GRB2 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

GRB2 - human gene knockout kit via CRISPR

GRB2 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

GRB2 - human gene knockout kit via CRISPR

GRB2 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

GRB2 - human gene knockout kit via CRISPR

GRB2 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

GRB2 / ASH (transcript variant 1)

  Recombinant protein of human growth factor receptor-bound protein 2 (GRB2), transcript variant 1  
GRB2  / ASH Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

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