Gremlin-1 / GREM1 antibody

Principal name

Gremlin-1 / GREM1 antibody

Alternative names for Gremlin-1 / GREM1 antibody

DAND2, CKTSF1B1, DRM, PIG2, IHG-2, GREM-1, DAN domain family member 2, Cysteine knot superfamily 1 BMP antagonist 1, Cell proliferation-inducing gene 2 protein

SwissProt ID

O35793 (Rat), O60565 (Human), O70326 (Mouse), O73754 (Xenla), O73755 (Chick)

Gene ID

26585 (GREM1), 23892 (Grem1)

Available reactivities

Hu (Human), Ms (Mouse), Rb (Rabbit), Rt (Rat)

Available hosts

Mouse, Rabbit, Rat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P)

Background of Gremlin-1 / GREM1 antibody

Gremlin, also known as “Increased in High Glucose protein 2” (IHG2) and “Down regulated in Mos-transformed cells protein” (Drm), is a 28 kDa member of the Dan family of secreted glycoproteins. Native human Gremlin consist of 160 amino acids. The mature region contains one potential site for N-linked glycosylation (Asn42), a cysteine-rich region, and a cysteine-knot motif (aa94-184) whose structure is shared by members of the TGFβ superfamily. Posttranslational modifications include glycosylation and phosphorylation (1-3). Human Gremlin exists in both secreted and membrane-associated forms (3) and there exist 2 isoforms. The aa sequence identity of human Gremlin with mouse and chicken Gremlin is 99% and 86%, respectively. Northern blot analysis shows that Gremlin mRNA is highly expressed in the small intestine, fetal brain and colon, and weakly expressed in adult brain, ovary, prostate, pancreas and skeletal muscle (4). Gremlin functions as a bone morphogenetic protein (BMP) antagonist. It acts by binding to, and forming heterodimers with, BMP2, BMP4, and BMP7, thus preventing them from interacting with their cell surface receptors (1). This mechanism is thought to be responsible for the pattern-inducing activity of Gremlin during embryonic development (5) and to play a role in human diseases, such as diabetic nephropathy (6). However, intracellular BMP-independent mechanisms of action (7) may mediate the ability of Gremlin to suppress transformation and tumor genesis under certain experimental conditions (8, 9). Gremlin also interacts with Slit proteins and acts as an inhibitor of monocyte chemotaxis (10). In addition, Gremlin has been found to be a proangiogenic factor expressed by endothelium (9). Furthermore Gremlin is a novel agonist of the major proangiogenic receptor VEGFR2 (11).

General readings

Kupfer, S.S., et al. Gastroenterology 139(5):1677-1685(2010)
Dimitrov, B.I., et al. J. Med. Genet. 47(8):569-574(2010)
McKnight, A.J., et al. J. Am. Soc. Nephrol. 21(5):773-781(2010)
van Vlodrop, I.J., et al. Am. J. Pathol. 176(2):575-584(2010)
Mangold, E., et al. Nat. Genet. 42(1):24-26(2010)

Items 1 to 20 of 37 total

per page
Page:
  1. 1
  2. 2

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Gremlin-1 / GREM1 (162-176) antibody

Anti-Gremlin-1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml. Rabbit Purified Hu E, P
50 µg / €345.00
  OriGene Technologies, Inc.

Gremlin-1 / GREM1 antibody

Predicted band size: 21 kDa . Positive control: Mouse testis tissue lysate. Recommended dilution: 1/500-2000. (Gel: 12%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/700 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Gremlin-1 / GREM1 antibody

Immunohistochemistry-Paraffin: Gremlin 1 Antibody [TA336479] - Detection of Gremlin in human colon cancer tissue using antibody at 5 ug/ml. Rabbit Purified Hu C, P
0.1 mg / €325.00
  OriGene Technologies, Inc.

Gremlin-1 / GREM1 antibody

Host: Rabbit; Target Name: Grem1; Sample Tissue: Rat Testis lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Hu, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Gremlin-1 / GREM1 (C-term) antibody

(TOP) Formalin-PBS and Bouin embedded paraffin human renal tissue pretreated with heat induced epitope retrieval Citrate buffer pH 6.0 in microwave. Dilution of primary antibody was 1:50. Detection method was ABC system or Vectastaint Elite VECTOR. (BUTTOM) Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with GREMLIN Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. Rabbit Ig Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Gremlin-1 / GREM1 (C-term) antibody

Immunohistochemistry with Gremlin 1 Polyclonal Antibody Rabbit IgG Aff - Purified Hu, Ms, Rb, Rt WB
0.1 mg / €340.00
  Acris Antibodies GmbH

Gremlin-1 / GREM1 antibody

Western analysis of anti-human Gremlin-1 antibody Cat.-No. AP26028PU-N. Samples were loaded in 15% SDS-polyacrylamide gel under reducing conditions. 
Lane 1: MWM (kDa) 
Lane 2: rm Grem1 
Lane 3: rh Grem1 
Lane 4: rh Grem1. Rabbit IgG Purified Hu WB
0.2 mg / €370.00
  Acris Antibodies GmbH

Gremlin-1 / GREM1 antibody

Western analysis of anti-human Gremlin-1 antibody Cat.-No. AP26028PU-N. Samples were loaded in 15% SDS-polyacrylamide gel under reducing conditions. 
Lane 1: MWM (kDa) 
Lane 2: rm Grem1 
Lane 3: rh Grem1 
Lane 4: rh Grem1. Rabbit IgG Purified Hu WB
0.1 mg / €270.00
  Acris Antibodies GmbH

Gremlin-1 / GREM1 antibody

Western analysis using anti-Mouse Gremlin-1 antibody Cat.-No AP26029PU-N. Samples were loaded in 15% SDS-polyacrylamide gel under reducing conditions.
Lane 1: MWM (kDa)
Lane 2: rm Grem1,
Lane 3: rm Grem1,
Lane 4: rh Grem1. Rabbit IgG Purified Hu, Ms WB
0.2 mg / €370.00
  Acris Antibodies GmbH

Gremlin-1 / GREM1 antibody

Western analysis using anti-Mouse Gremlin-1 antibody Cat.-No AP26029PU-N. Samples were loaded in 15% SDS-polyacrylamide gel under reducing conditions.
Lane 1: MWM (kDa)
Lane 2: rm Grem1,
Lane 3: rm Grem1,
Lane 4: rh Grem1. Rabbit IgG Purified Hu, Ms WB
0.1 mg / €270.00
  Acris Antibodies GmbH

Gremlin-1 / GREM1 (C-term) antibody

Western blot analysis of GREM1 (arrow) using Gremlin-1 / GREM1 Antibody (C-term) Cat.-No AP51947PU-N. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the GREM1 gene. Rabbit Ig Aff - Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

Gremlin-1 / GREM1 antibody

Gremlin-1 / GREM1 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Gremlin-1 / GREM1 antibody

Gremlin-1 / GREM1 Mouse IgG2a 4D8 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

Gremlin-1 / GREM1 antibody

Gremlin-1 / GREM1 Mouse IgM 2C8 Ascites Hu E
0.2 ml / €370.00
  Abnova Taiwan Corp.

Gremlin-1 / GREM1 antibody

Gremlin-1 / GREM1 Mouse IgM 2C9 Ascites Hu E
0.2 ml / €370.00
  Abnova Taiwan Corp.

Gremlin-1 / GREM1 antibody

Detection limit for recombinant GST tagged GREM1 is approximately 3ng/ml as a capture antibody. Mouse IgG2a 4C2 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Gremlin-1 / GREM1 antibody

Detection limit for recombinant GST tagged GREM1 is approximately 0.03ng/ml as a capture antibody. Mouse IgG2a 3H4 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

Gremlin-1 / GREM1 antibody

Detection limit for recombinant GST tagged GREM1 is approximately 0.3ng/ml as a capture antibody. Mouse IgG2a 2F1 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Gremlin-1 / GREM1 antibody

Detection limit for recombinant GST tagged GREM1 is approximately 1ng/ml as a capture antibody. Mouse IgG2a 2D1 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Gremlin-1 / GREM1 antibody

Formalin-fixed and paraffin-embedded human skeletal muscle tissue reacted with GREM1 polyclonal antibody ( Cat # PAB4716 ) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. Rabbit Hu, Ms E, P, WB
0.4 ml / €390.00
  Abnova Taiwan Corp.

Items 1 to 20 of 37 total

per page
Page:
  1. 1
  2. 2
  • LinkedIn