GRIP1 antibody

Principal name

GRIP1 antibody

Alternative names for GRIP1 antibody

GRIP-1, Glutamate receptor-interacting protein 1

SwissProt ID

P97879 (Rat), Q925T6 (Mouse), Q9Y3R0 (Human)

Gene ID

23426 (GRIP1)

Available reactivities

Hst (Hamster), Hu (Human), Ms (Mouse), Rt (Rat), Ze (Zebrafish), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Frozen Sections (C), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P)

Background of GRIP1 antibody

NCOA2/GRIP1 (nuclear receptor coactivator 2/glucocorticoid receptor-interacting protein 1 (GRIP1)) is a 159 kD member of the p160/steroid receptor coactivator family containing a nuclear localization sequence, bHLH, and PAS domain. Two isoforms of this protein have been reported. NCOA/GRIP1 is expressed in the cytoplasm and translocated into the nucleus upon phosphorylation. This protein is highly expressed in the placenta, skeletal muscle, uterus with lower expression in B cells, lymphocytes, and some tumors. NCOA/GRIP1 has histone acetyltransferase activity, and is a transcriptiol coactivator for steroid and nuclear hormone receptor. This protein mediates ligand-independent nuclear translocation and activates the CAR receptor in response to phenobarbital. This protein is modified by phosphorylation. NCOA/GRIP1 forms a multisubunit coactivation complex with NCOA3, IKKA, IKKB, IKBKG and histone acetyltransferase protein CREB binding protein and has also been shown to interact with HIF1A, APEX, NR3C1, and CAR. The Poly6090 antibody recognizes the N-termil region of human NCOA2/GRIP1 and has been shown to be useful for Western blotting.

General readings

General / background references:Hong H et al. An additional region of coactivator GRIP1 required for interaction with the hormone-binding domains of a subset of nuclear receptors. J Biol Chem 274:3496-502 (1999). PubMed PMID: 9920895
Schmidt S et al. Multiple receptor interaction domains of GRIP1 function in synergy. Nucleic Acids Res 26:1191-7 (1998). PubMed PMID: 9469826
Hong H et al. GRIP1, a transcriptional coactivator for the AF-2 transactivation domain of steroid, thyroid, retinoid, and vitamin D receptors. Mol Cell Biol 17:2735-44 (1997). PubMed PMID: 9111344

12 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GRIP1 antibody

Western blot analysis of GRIP1 in HeLa cell lysate with GRIP1 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GRIP1 antibody

Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-NCOA2/GRIP1 polyclonal antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. Rabbit IgG Poly6090 Purified Hu WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

GRIP1 antibody

WB Suggested Anti-GRIP1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: HepG2 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

GRIP1 antibody

GRIP1 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

GRIP1 antibody

GRIP1 Mouse IgG2a 4A9 Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

GRIP1 antibody

Human Muscle; WB Suggested Anti-GRIP1 Antibody Titration: 1.25ug/ml. Positive Control: Human Muscle; GRIP1 antibody - N-terminal region (ARP32496_T100) in Human Muscle cells using Western Blot Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €310.00
  AVIVA Systems Biology

GRIP1 (C-term) antibody

Human HepG2; WB Suggested Anti-GRIP1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: HepG2 cell lysate; GRIP1 antibody - C-terminal region (ARP32497_P050) in Human HepG2 cells using Western Blot Rabbit Aff - Purified Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

GRIP1 antibody

Figure 1 shows immunolocalization of GRIP1 in CHO cells using NB120-2842. Rabbit IgG Purified Hu ICC/IF
0.1 mg / €400.00
  Novus Biologicals Inc.

GRIP1 antibody

Immunohistochemistry-Paraffin: GRIP1 Antibody [NBP1-86252] Immunohistochemical staining of human pancreas shows strong cytoplasmic positivity in exocrine pancreas. Rabbit IgG Aff - Purified Hu P
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

GRIP1

GRIP1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

GRIP1

GRIP1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

GRIP1 overexpression lysate

GRIP1 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

12 Item(s)

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