GRK4 antibody

Principal name

GRK4 antibody

Alternative names for GRK4 antibody

G protein-coupled receptor kinase 4, ITI1, GPRK2L, GPRK4, G protein-coupled receptor kinase GRK4

SwissProt ID

O70291 (Mouse), P32298 (Human), P70507 (Rat), Q15313 (Human)

Gene ID

2868 (GRK4)

Available reactivities

Hu (Human), Bov (Bovine), Can (Canine), Chk (Chicken), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB)

Background of GRK4 antibody

This gene encodes a member of the guanine nucleotide-binding protein (G protein)-coupled receptor kise subfamily of the Ser/Thr protein kise family. The protein phosphorylates the activated forms of G protein-coupled receptors thus initiating its deactivation. This gene has been linked to both genetic and acquired hypertension. Specifically phosphorylates the activated forms of G protein-coupled receptors. GRK4-alpha can phosphorylate rhodopsin and its activity is inhibited by calmodulin; the other three isoforms do not phosphorylate rhodopsin and do not interact with calmodulin. GRK4-alpha and GRK4-gamma phosphorylate DRD3. Phosphorylates ADRB2.

General readings

Staessen,J.A., (er) Hypertension (2008) In press

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GRK4 antibody

Predicted band size: 66 kDa . Positive control: Jurkat cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 10 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GRK4 antibody

Predicted band size: 66 kDa . Positive control: Hela and Jurkat cell lysate. Recommended dilution: 1/1000-5000. (Gel: 10%SDS-PAGE Lane1: Hela cell lysate Lane2: Jurkat cell lysate Lysates: 40 ug per lane Primary antibody: 1/700 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 40 seconds) Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GRK4 antibody

WB Suggested Anti-GRK4 Antibody Titration: 0.2-1 ug/ml; Positive Control: Jurkat cell lysate Rabbit IgG Purified Can, Eq, GP, Hu, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

GRK4 antibody

WB Suggested Anti-GRK4 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 312500; Positive Control: MCF7 cell lysate Rabbit IgG Purified Can, Eq, GP, Hu, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

GRK4 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRK4 (RC216602, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRK4.(1:500) Mouse IgG1 OTI4F5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GRK4 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GRK4 (RC216602, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GRK4. Mouse IgG2a OTI9A3 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GRK4 antibody

GRK4 polyclonal antibody (A01), Lot # 060516JCS1 Western Blot analysis of GRK4 expression in HL-60 ( Cat # L014V1 ). Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

GRK4 antibody

GRK4 Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

GRK4 antibody

Detection limit for recombinant GST tagged GRK4 is approximately 0.3ng/ml as a capture antibody. Mouse IgG2b 6D12 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

GRK4 antibody

GRK4 Mouse IgG2b 6D12 Ascites Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

GRK4 antibody

Detection limit for recombinant GST tagged GRK4 is approximately 0.1ng/ml as a capture antibody. Mouse IgG2b 6D5 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

GRK4 antibody

Detection limit for recombinant GST tagged GRK4 is approximately 0.1ng/ml as a capture antibody. Mouse IgG1 2C5 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

GRK4 antibody

Detection limit for recombinant GST tagged GRK4 is approximately 0.3ng/ml as a capture antibody. Mouse IgG1 4D2 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

GRK4 antibody

GRK4 Mouse IgG2b 1F7 Ascites Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

GRK4 antibody

Human Jurkat; WB Suggested Anti-GRK4 Antibody Titration: 0.2-1 ug/ml. Positive Control: Jurkat cell lysate; GRK4 antibody - middle region (ARP53565_P050) in Human Jurkat cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Chk, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

GRK4 antibody

GRK4 Rabbit Aff - Purified Hu WB
50 µg / €350.00
  AVIVA Systems Biology

GRK4 antibody

Western Blot: GRK4 Antibody [NBP1-55313] - This Anti-GRK4 antibody was used in Western Blot of Jurkat tissue lysate at a concentration of 1ug/ml. Rabbit Aff - Purified Hu WB
50 µg / €440.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

GRK4 (transcript variant 1)

GRK4 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

GRK4

GRK4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

GRK4

GRK4 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

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