Grp75 / HSPA9 antibody

Principal name

Grp75 / HSPA9 antibody

Alternative names for Grp75 / HSPA9 antibody

Heat shock 70 kDa protein 9, Peptide-binding protein 74, PBP74, MOT, HSPA9B, Stress-70 protein, Mortalin, 75 kDa glucose-regulated protein

SwissProt ID

P38646 (Human), P38647 (Mouse), P48721 (Rat), Q3ZCH0 (Bovin), Q5ZM98 (Chick)

Gene ID

3313 (HSPA9), 15526 (Hspa9)

Available reactivities

Bov (Bovine), Can (Canine), GP (Guinea Pig), Hst (Hamster), Hu (Human), Mky (Monkey), Ms (Mouse), Por (Porcine), Rb (Rabbit), Rt (Rat), Sh (Sheep), Xen (Xenopus), Dros (Drosophila), African clawed frog

Available hosts

Mouse, Rabbit

Available applications

Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), ELISA (detection) (E(detection)), Flow Cytometry (F)

Background of Grp75 / HSPA9 antibody

In response to adverse changes in their environment, cells from many organisms increase the expression of a class of proteins referred to as heat shock or stress proteins. One class of stress proteins, termed the Hsp70 family, is comprised of multiple members, all of which bind ATP in vitro, but which are localized within different intracellular compartments. These include: i) Hsc70 (or constitutive form) present within the cytosol/nucleus; ii) Hsp70 (inducible form) present within the cytosol/nucleus/nucleolus; iii) the constitutive glucose-regulated 78 kDa (or BiP) protein present within the lumen of the endoplasmic reticulum; and iv) the constitutive glucose regulated 75 kDa protein present within the mitochondrial matrix. Members of the Hsp70 family are thought to function as molecular chaperones, assisting in the folding of other proteins in various intracellular compartments. Grp75 is localized in the mitochondrial matrix, where, in concert with Hsp60, is thought to participate in the refolding of proteins translocated into this organelle. Like its E. coli homolog DnaK, Grp75 possesses a cation dependent ATPase activity thought to be central to its function as a chaperone.
The HSP 70 family is a set of highly conserved proteins that are induced by a variety of biological stresses, including heat stress, in every organism in which the proteins have been examined. The human HSP 70 family members include: HSP 70, a 70 kDa protein which is strongly inducible in all organisms but which is also constitutively expressed in primate cells; HSP 72, a 72 kDa protein which is induced exclusively under stress conditions; HSC 70, or cognate protein, is a 72 kDa constitutively expressed protein which is involved in uncoating clathrin-coated vesicles; GRP 78, or BiP, is a glucose-regulated 78 kDa protein localized to the endoplasmic reticulum; and mitochondrial HSP 70 (mtHSP 70, GRP 75 or mortalin) a 75 kDa protein that is found within the mitochondria.
mtHSP 70 is a mitochondrial resident protein that is involved in protein translocation into the mitochondria. Preproteins cross the mitochondrial membranes in an extended conformation. This requires unfolding of preproteins before entering translocation pores in the mitochondrial outer membrane. Preprotein unfolding is thought to be mediated by mtHSP 70 and other inner membrane proteins of the mitochondria.

General readings

1. Chen, T. H.-P., Kambal, A., Krysiak, K., Walshauser, M. A., Raju, G., Tibbitts, J. F., Walter, M.
J. Knockdown of Hspa9, a del(5q31.2) gene, results in a decrease in hematopoietic progenitors in
mice. Blood 117: 1530-1539, 2011.
2. Gross, M. B. Personal Communication. Baltimore, Md. 9/12/2011.
3. Kaul, S. C., Wadhwa, R., Matsuda, Y., Hensler, P. J., Pereira-Smith, O. M., Komatsu, Y., Mitsui,
Y. Mouse and human chromosomal assignments of mortalin, a novel member of the murine hsp70
family of proteins. FEBS Lett. 361: 269-272, 1995.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GRP75 antibody

Immunohistochemical analysis of GRP75 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

GRP75 antibody

Immunohistochemical analysis of GRP75 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

GRP75 antibody

Immunohistochemical analysis of GRP75 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

Grp75 / HSPA9 antibody

Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-HSPA9 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500516) Mouse IgG2b OTI9F8 Purified Can, Hu, Mky, Rt F, ICC/IF, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Grp75 / HSPA9 antibody

Immunofluorescent staining of HT29 cells using anti-HSPA9  mouse monoclonal antibody (TA500548). Mouse IgG2a OTI4A1 Purified Can, Hu, Mky, Rt ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Grp75 / HSPA9 antibody

Human Skeletal muscle; Skeletal muscle; HSPA9 antibody - C-terminal region (ARP30175_P050) in Human Skeletal muscle cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Can, Dros, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

Grp75 / HSPA9 antibody

Immunohistochemistry-Paraffin: Grp75 Antibody (9F8) [NBP1-47801] - Immunohistochemical staining of paraffin-embedded pancreas tissue using antiHSPA9mouse monoclonal antibody. (Dilution 1:50) Mouse 9F8 Aff - Purified Can, Hu, Mky, Rt F, ICC/IF, P, WB
0.1 ml / €620.00
  Novus Biologicals Inc.

Grp75 / HSPA9 (661-679) antibody

Human colon ca. stained with Anti-GRP75, cat# AP15586PU Rabbit Purified Hu, Ms, Rt P
0.5 ml / €360.00
  Acris Antibodies GmbH

Grp75 / HSPA9 (661-679) antibody

Human colon ca. stained with Anti-GRP75, cat# AP15586PU Rabbit Purified Hu, Ms, Rt P
1 ml / €520.00
  Acris Antibodies GmbH

Grp75 / HSPA9 (661-679) antibody

Human colon ca. stained with Anti-GRP75, cat# AP15586PU Rabbit Purified Hu, Ms, Rt P
0.1 ml / €190.00
  Acris Antibodies GmbH

Grp75 / HSPA9 antibody

Predicted band size: 74 kDa . Positive control: MCF7, K562, HeLa and Jurkat cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lane1: MCF7 cell lysate Lane2: K562 cell lysate Lane3: HeLa cell lysate Lane4: Jurkat cell lysate Lysates: 40 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Grp75 / HSPA9 antibody

Western blot analysis of Grp75 in HeLa cell lysates using a 1:1000 dilution of the antibody Mouse IgG1 S52A-42 Purified Hu, Ms, Rt C, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Grp75 / HSPA9 antibody

Western blot analysis of extracts of various cell lines, using HSPA9 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

Grp75 / HSPA9 antibody

Western blot analysis on COS7 cell lysate using GRP75 Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Grp75 / HSPA9 antibody

Western blot analysis of GRP75 expression in Cos7 cell extract Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Grp75 / HSPA9 antibody

Anti-HSPA9 mouse monoclonal antibody (TA500630) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY HSPA9(RC201397). Mouse IgG1 OTI10D7 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Grp75 / HSPA9 antibody

adult rat hippocampus immunohistochemistry Mouse IgG1 N52A/42 Supernatant Hu, Ms, Rt C, IP, WB
5 ml / €320.00
  Antibodies Incorporated

NeuroMab

Grp75 / HSPA9 antibody

adult rat hippocampus immunohistochemistry Mouse IgG1 N52A/42 Purified Hu, Ms, Rt C, IP, WB
0.1 ml / €380.00
  Antibodies Incorporated

NeuroMab

Grp75 / HSPA9 antibody

Western blot analysis of Grp75 in HeLa cell lysates using a 1:1000 dilution of the antibody Mouse IgG1 S52A-42 Purified Hu, Ms, Rt C, IP, WB
25 µg / €230.00
  Acris Antibodies GmbH

Grp75 / HSPA9 antibody

Immunohistochemistry (IHC) analysis of GRP75/HSPA9
antibody (Cat.-No.: AP06148PU-N) in paraffin-embedded human breast carcinoma
tissue. Rabbit Aff - Purified Hu, Ms, Rt E, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

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