GSK3 alpha antibody

Principal name

GSK3 alpha antibody

Alternative names for GSK3 alpha antibody

Glycogen synthase kinase-3 alpha, GSK3A, GSK-3 alpha, Factor A

SwissProt ID

P18265 (Rat), P49840 (Human), Q2NL51 (Mouse)

Gene ID

2931 (GSK3A), 50686 (Gsk3a), 2932 (GSK3B), 606496

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Bov (Bovine), Mky (Monkey), Por (Porcine), Rb (Rabbit), Sh (Sheep), Chk (Chicken), Chimp (Chimpanzee), Ze (Zebrafish), Xen (Xenopus)

Available hosts

Rabbit, Mouse, Chicken

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Frozen Sections (C)

Background of GSK3 alpha antibody

Glycogen synthase kinase 3 (GSK3) is a proline directed serine threonine kinase that was initially identified as a phosphorylating and inactivating glycogen synthase, a key enzyme in glycogen metabolism. Since then, it has been shown to be involved in the regulation of a diverse array of cellular functions, including protein synthesis, cell proliferation, cell differentiation, microtubule assembly/disassembly, and apoptosis. GSK3s substrate specificity is unique in that phosphorylation of substrate only occurs if a phosphoserine or phosphotyrosine is present four residues C terminal to the site of GSK phosphorylation. There exists two isoforms of GSK3, alpha and beta, and they show a high degree of amino acid homology. The two isoforms of GSK3 are strictly regulated via phosphorylation. Phosphorylation of GSK3 beta on Ser9 (Ser21 in GSK3 alpha) by protein kinase B (PKB) causes its inactivation is the primary mechanism responsible for growth factor inhibition of this kinase. Activation of GSK3 beta is dependent upon the phosphorylation of Tyr216 (Tyr279 in GSK3 alpha). Upon activation, it has been shown to phosphorylate a number of different cellular proteins, including p53, c-Myc, c-Jun, heat shock factor 1 (HSF1), and cyclin D1. GSK3 beta also has been shown to phosphorylate aberrant sites on the microtubule associated protein tau, which is critical for the progression of Alzheimer's disease. GSK3B is involved in energy metabolism, neuronal cell development, and body pattern formation. GSK3 (Glycogen Synthase Kinase-3 differs from most serine/threonine kinases) in that it is active in the absence of the action of signaling pathways. Two isoforms exist, GSK-3a and GSK-3ß. The function of GSK-3 is to phosphorylate glycogen synthase and thereby inactivate it. Insulin action stimulates the PI 3-kinase pathway, resulting in Akt activation, which phosphorylates and inactivates GSK-3.

General readings

1. Darrington RS  et al. Distinct expression and activity of GSK-3a and GSK-3ß in prostate cancer. Int J Cancer 131:E872-83 (2012).
2. Campa VM & Kypta RM Issues associated with the use of phosphospecific antibodies to localise active and inactive pools of GSK-3 in cells. Biol Direct 6:4 (2011). WB, ICC/IF ; Human .
3. Lauing KL  et al. Exogenous Activation of Wnt/ß-Catenin Signaling Attenuates Binge Alcohol-Induced Deficient Bone Fracture Healing. Alcohol Alcohol N/A:N/A (2014). Mouse .
4. Durak O  et al. Ankyrin-G regulates neurogenesis and Wnt signaling by altering the subcellular localization of ß-catenin. Mol Psychiatry N/A:N/A (2014).
5. Shanmugam V  et al. Whole genome sequencing reveals potential targets for therapy in patients with refractory KRAS mutated metastatic colorectal cancer. BMC Med Genomics7:36 (2014). WB .
6. Jin R  et al. The metastasis suppressor NDRG1 modulates the phosphorylation and nuclear translocation of ß-catenin through mechanisms involving FRAT1 and PAK4. J Cell Sci127:3116-30 (2014). Human .
7. Rui Y  et al. Activity-dependent regulation of dendritic growth and maintenance by glycogen synthase kinase 3ß. Nat Commun 4:2628 (2013).
8. Marques JM  et al. CRMP2 tethers kainate receptor activity to cytoskeleton dynamics during neuronal maturation. J Neurosci 33:18298-310 (2013).
9. Ohmi K  et al. Defects in the medial entorhinal cortex and dentate gyrus in the mouse model of Sanfilippo syndrome type B. PLoS One 6:e27461 (2011). IHC-Fr ; Mouse .
10. Ramirez SH  et al. Inhibition of glycogen synthase kinase 3beta (GSK3beta) decreases inflammatory responses in brain endothelial cells. Am J Pathol 176:881-92 (2010). IHC-Fr ; Human .

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

G3 alpha/beta antibody

Immunohistochemical analysis of GSK3 alpha/beta staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms, Rt, Ze P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

G3 alpha/beta antibody

Immunohistochemical analysis of GSK3 alpha/beta staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms, Rt, Ze P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

G3 alpha/beta antibody

Immunohistochemical analysis of GSK3 alpha/beta staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms, Rt, Ze P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

G3 alpha/beta pTyr279/pTyr216 antibody

Immunohistochemical analysis of GSK3 alpha/beta (pY279/216) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms, Rt, Ze P, WB
30 µl / €210.00
  Cohesion Biosciences Ltd.

G3 alpha/beta pTyr279/pTyr216 antibody

Immunohistochemical analysis of GSK3 alpha/beta (pY279/216) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms, Rt, Ze P, WB
0.1 ml / €330.00
  Cohesion Biosciences Ltd.

G3 alpha/beta pTyr279/pTyr216 antibody

Immunohistochemical analysis of GSK3 alpha/beta (pY279/216) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms, Rt, Ze P, WB
0.2 ml / €530.00
  Cohesion Biosciences Ltd.

GSK3 alpha antibody

Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using GSK3α antibody (AP02551PU-S; AP02551PU-N). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €270.00
  Acris Antibodies GmbH

GSK3 alpha antibody

Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using GSK3α antibody (AP02551PU-S; AP02551PU-N). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
50 µl / €230.00
  Acris Antibodies GmbH

GSK3 alpha (+ beta) antibody

Human brain stained with Anti-GSK-3 antibody Rabbit Purified Chk, Hu, Mky, Ms, Rt P
0.5 ml / €360.00
  Acris Antibodies GmbH

GSK3 alpha (+ beta) antibody

Human brain stained with Anti-GSK-3 antibody Rabbit Purified Chk, Hu, Mky, Ms, Rt P
1 ml / €520.00
  Acris Antibodies GmbH

GSK3 alpha (+ beta) antibody

Human brain stained with Anti-GSK-3 antibody Rabbit Purified Hu, Mky, Ms, Rt P
0.1 ml / €190.00
  Acris Antibodies GmbH

GSK3 alpha antibody

Western blot analysis of extracts from K562/HepG2/RAW264.7 cells, using GSK3 alpha (Ab-21) Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GSK3 alpha pSer21 antibody

WB using Anti-GSK3A antibody shows detection of a 52 kDa band corresponding to human GSK3A in various human derived 293T cell extracts. Lane 1: control, Lane 2: treated with IGF-1 (100 ng/ml) for 20 min, Lane 3: pre-treated with 10 uM LY294002 (selective PI3K inhibitor) and treated with IGF-1 (100 ng/ml) for 20 min.  Lane 1 shows some baseline GSK3A reactivity that is intensified upon stimulation (lane2) and diminished when an inhibitor is added (lane 3).  Primary antibody was used at 1:1,000. Rabbit Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GSK3 alpha antibody

WB using Anti-GSK3A antibody shows detection of a 52 kDa band corresponding to human GSK3A present in ~ 35 µg of HEK293 whole cell lysate (lane 1). Primary antibody was used at a 1:1,000 dilution in blocking buffer and reacted overnight at 4?. The membrane was washed and reacted with a 1:10,000 dilution of IRDye800™ conjugated Gt-a-Rabbit IgG [H&L] MX  for 45 min at RT. Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). Rabbit Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GSK3 alpha antibody

Predicted cell location: Cytoplasm, Nucleus. Positive control: Human colon and ovarian cancer tissue. Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded Human colon and ovarian cancer tissue using GSK3A antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

GSK3 alpha antibody

Predicted cell location: Cytoplasm. Positive control: Human colon and renal cancer tissue. Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded Human colon and renal cancer tissue using GSK3A antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

GSK3 alpha antibody

Mouse Gsk3a Antibody (C-term) (Cat. #TA324569) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the Gsk3a antibody detected the Gsk3a protein (arrow). Rabbit IgG Purified Ms P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

GSK3 alpha (C-term) antibody

Western blot analysis of GSK3A (arrow) using GSK3A Antibody (C-term) (Cat.#TA325026). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GSK3A gene (Lane 2) (Origene Technologies). Rabbit Ig Purified Hu P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

GSK3 alpha antibody

Western blot analysis of GSK3 alpha expression in ovarycancer whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GSK3 alpha antibody

Western blot analysis of GSK3 lpha/beta expression in TNF-a treated 293 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

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