GSK3 alpha antibody

Principal name

GSK3 alpha antibody

Alternative names for GSK3 alpha antibody

Glycogen synthase kinase-3 alpha, GSK3A, GSK-3 alpha, Factor A

SwissProt ID

P18265 (Rat), P49840 (Human), Q2NL51 (Mouse)

Gene ID

2931 (GSK3A), 606496 (Gsk3a)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Bov (Bovine), Mky (Monkey), Chk (Chicken), Chimp (Chimpanzee), Ze (Zebrafish)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Frozen Sections (C)

Background of GSK3 alpha antibody

Glycogen synthase kinase 3-alpha (GSK3A)is a multifunctional protein serine kinase implicated in the control of several regulatory proteins including glycogen synthase and transcription factors. It also plays a role in the WNT and PI3K signaling pathways.1 Under resting conditions GSK3A and its homologs are highly phosphorylated at tyr279 in the phosphorylation loop.2 Constitutive phosphorylation of this tyrosine is important for kinase activity. Dephosphorylation of tyr279 after mitogen activation is accompanied by kinase inactivation. PKA as well as PI3K-activated PKB inactivate GSK3A by phosphorylation at ser21.3 Lysophosphatidic acid primarily utilizes a PKC-dependent pathway to modulate GSK3 and certain growth factors (e.g., PDGFB), which control GSK3 mainly through PIK3-PKB, are able to regulate GSK3 through an alternative, redundant phospholipase-C-gamma-PKC pathway.4 Alzheimer disease (AD) is associated with increased production and aggregation of amyloid-beta-40 and -42 peptides into plaques. GSK3A is required for maximal production of the beta-amyloid-40 and -42 peptides generated from the amyloid precursor protein (APP) by presenilin (PSEN1)-dependent gamma-secretase cleavage.5 In vitro, lithium, a GSK3A inhibitor, blocked the production of the beta-amyloid peptides by interfering with the gamma-secretase step. In mice expressing familial AD-associated mutations in APP and PSEN1, lithium reduced the levels of beta-amyloid peptides GSK3A also phosphorylates the tau protein (MAPT), the principal component of neurofibrillary tangles in AD, and suggested that inhibition of GSK3A may offer a new therapeutic approach to AD.

General readings

1. Darrington RS  et al. Distinct expression and activity of GSK-3a and GSK-3ß in prostate cancer. Int J Cancer 131:E872-83 (2012).
2. Campa VM & Kypta RM Issues associated with the use of phosphospecific antibodies to localise active and inactive pools of GSK-3 in cells. Biol Direct 6:4 (2011). WB, ICC/IF ; Human .
3. Lauing KL  et al. Exogenous Activation of Wnt/ß-Catenin Signaling Attenuates Binge Alcohol-Induced Deficient Bone Fracture Healing. Alcohol Alcohol N/A:N/A (2014). Mouse .
4. Durak O  et al. Ankyrin-G regulates neurogenesis and Wnt signaling by altering the subcellular localization of ß-catenin. Mol Psychiatry N/A:N/A (2014).
5. Shanmugam V  et al. Whole genome sequencing reveals potential targets for therapy in patients with refractory KRAS mutated metastatic colorectal cancer. BMC Med Genomics7:36 (2014). WB .
6. Jin R  et al. The metastasis suppressor NDRG1 modulates the phosphorylation and nuclear translocation of ß-catenin through mechanisms involving FRAT1 and PAK4. J Cell Sci127:3116-30 (2014). Human .
7. Rui Y  et al. Activity-dependent regulation of dendritic growth and maintenance by glycogen synthase kinase 3ß. Nat Commun 4:2628 (2013).
8. Marques JM  et al. CRMP2 tethers kainate receptor activity to cytoskeleton dynamics during neuronal maturation. J Neurosci 33:18298-310 (2013).
9. Ohmi K  et al. Defects in the medial entorhinal cortex and dentate gyrus in the mouse model of Sanfilippo syndrome type B. PLoS One 6:e27461 (2011). IHC-Fr ; Mouse .
10. Ramirez SH  et al. Inhibition of glycogen synthase kinase 3beta (GSK3beta) decreases inflammatory responses in brain endothelial cells. Am J Pathol 176:881-92 (2010). IHC-Fr ; Human .

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

G3 alpha/beta antibody

Immunohistochemical analysis of GSK3 alpha/beta staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms, Rt, Ze P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

G3 alpha/beta antibody

Immunohistochemical analysis of GSK3 alpha/beta staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms, Rt, Ze P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

G3 alpha/beta antibody

Immunohistochemical analysis of GSK3 alpha/beta staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms, Rt, Ze P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

G3 alpha/beta pTyr279/pTyr216 antibody

Immunohistochemical analysis of GSK3 alpha/beta (pY279/216) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms, Rt, Ze P, WB
30 µl / €210.00
  Cohesion Biosciences Ltd.

G3 alpha/beta pTyr279/pTyr216 antibody

Immunohistochemical analysis of GSK3 alpha/beta (pY279/216) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms, Rt, Ze P, WB
0.1 ml / €330.00
  Cohesion Biosciences Ltd.

G3 alpha/beta pTyr279/pTyr216 antibody

Immunohistochemical analysis of GSK3 alpha/beta (pY279/216) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. Rabbit Aff - Purified Hu, Ms, Rt, Ze P, WB
0.2 ml / €530.00
  Cohesion Biosciences Ltd.

GSK3 alpha antibody

Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using GSK3α antibody (AP02551PU-S; AP02551PU-N). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €270.00
  Acris Antibodies GmbH

GSK3 alpha antibody

Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using GSK3α antibody (AP02551PU-S; AP02551PU-N). Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
50 µl / €230.00
  Acris Antibodies GmbH

GSK3 alpha (+ beta) antibody

Human brain stained with Anti-GSK-3 antibody Rabbit Purified Chk, Hu, Mky, Ms, Rt P
0.5 ml / €360.00
  Acris Antibodies GmbH

GSK3 alpha (+ beta) antibody

Human brain stained with Anti-GSK-3 antibody Rabbit Purified Chk, Hu, Mky, Ms, Rt P
1 ml / €530.00
  Acris Antibodies GmbH

GSK3 alpha (+ beta) antibody

Human brain stained with Anti-GSK-3 antibody Rabbit Purified Hu, Mky, Ms, Rt P
0.1 ml / €200.00
  Acris Antibodies GmbH

GSK3 alpha antibody

Western blot analysis of extracts from K562/HepG2/RAW264.7 cells, using GSK3 alpha (Ab-21) Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GSK3 alpha pSer21 antibody

WB using Anti-GSK3A antibody shows detection of a 52 kDa band corresponding to human GSK3A in various human derived 293T cell extracts. Lane 1: control, Lane 2: treated with IGF-1 (100 ng/ml) for 20 min, Lane 3: pre-treated with 10 uM LY294002 (selective PI3K inhibitor) and treated with IGF-1 (100 ng/ml) for 20 min.  Lane 1 shows some baseline GSK3A reactivity that is intensified upon stimulation (lane2) and diminished when an inhibitor is added (lane 3).  Primary antibody was used at 1:1,000. Rabbit Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GSK3 alpha antibody

WB using Anti-GSK3A antibody shows detection of a 52 kDa band corresponding to human GSK3A present in ~ 35 µg of HEK293 whole cell lysate (lane 1). Primary antibody was used at a 1:1,000 dilution in blocking buffer and reacted overnight at 4?. The membrane was washed and reacted with a 1:10,000 dilution of IRDye800™ conjugated Gt-a-Rabbit IgG [H&L] MX  for 45 min at RT. Molecular weight estimation was made by comparison to prestained MW markers in lane M (700 nm channel, red). Rabbit Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GSK3 alpha antibody

Predicted band size: 51 kDa . Positive control: 293 cells untreated or treated with serum lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1: 293 cells untreated with serum lysate Lane2: 293 cells treated with serum lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GSK3 alpha antibody

Predicted cell location: Cytoplasm, Nucleus. Positive control: Human colon and ovarian cancer tissue. Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded Human colon and ovarian cancer tissue using GSK3A antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

GSK3 alpha antibody

Predicted cell location: Cytoplasm. Positive control: Human colon and renal cancer tissue. Recommended dilution: 1/50-200  The image on the left is immunohistochemistry of paraffin-embedded Human colon and renal cancer tissue using GSK3A antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

GSK3 alpha antibody

Mouse Gsk3a Antibody (C-term) (Cat. #TA324569) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the Gsk3a antibody detected the Gsk3a protein (arrow). Rabbit IgG Purified Ms P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

GSK3 alpha (C-term) antibody

Western blot analysis of GSK3A (arrow) using GSK3A Antibody (C-term) (Cat.#TA325026). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the GSK3A gene (Lane 2) (Origene Technologies). Rabbit Ig Purified Hu P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

GSK3 alpha antibody

Western blot analysis of GSK3 alpha expression in ovarycancer whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

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