GSK3 beta antibody

Principal name

GSK3 beta antibody

Alternative names for GSK3 beta antibody

GSK-3 beta, Glycogen synthase kinase-3 beta, GSK3B

SwissProt ID

P18266 (Rat), P49841 (Human), Q9WV60 (Mouse)

Gene ID

2932 (GSK3B), 84027 (Gsk3b), 56637

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Bov (Bovine), Chk (Chicken), Mky (Monkey), Xen (Xenopus), Ze (Zebrafish), Sh (Sheep), Frog, Por (Porcine), African clawed frog, Rb (Rabbit), Prim (Primate)

Available hosts

Rabbit, Mouse, Chicken

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Frozen Sections (C), PLA/Proximity ligation assay (PLA), Flow Cytometry (F)

Background of GSK3 beta antibody

Glycogen synthase kise 3 (GSK3) is a proline-directed serine/threonine protein kise that was origilly identified for its role in glycogen metabolism; however, subsequently it was shown to serve pleiotropic functions, including embryonic development, gene transcription, and neurol cell function. Mammalian GSK has two isoforms encoded by distinct genes: GSK3A produces a 51-kD GSK3-alpha protein and GSK3B a 47-kD GSK3-beta protein. Phosphorylation of Tyr279/Tyr216 on GSK3alpha/beta is important for enzymatic activity, whereas ictivation of GSK3 can be achieved through phosphorylation of Ser21/Ser9 residues within the N-termil domain on GSK3-alpha/beta, respectively. Upstream kises PKA, AKT/PKB, PKC, p90RSK/MAPKAP and p70S6K are known to phosphorylate Ser9 of GSK3-beta. Furthermore, GSK3 is also regulated via interactions with WNT ligand and its receptor Frizzled/co-receptor LRP5/6, and this interaction releases GSK3 from a multi-protein complex formed by beta-catenin, axin, and adenomatous polyposis coli (APC), which prevents GSK3-mediated beta-catenin degradation and induces beta-catenin-dependent gene transcription. GSK3 is one of the few sigling mediators that play central roles in a diverse range of sigling pathways, including those activated by WNT, hedgehog, growth factors, cytokines, and G protein-coupled ligands. GSK3 dysregulation, particularly hyperactivation, with various pathological conditions, including diabetes mellitus, obesity, inflammation, neurological disorders, and tumorigenesis.

General readings

Chin PC, et al. Brain Res Mol Brain Res 2005 Jun 13; 137(1-2): 193-201
Takahashi-Yanaga F, et al. Biochem Biophys Res Commun 2004 Apr 02; 316(2): 411-415
Fan G, et al. J Biol Chem 2003 Dec 26; 278(52): 52432-52436
Liao X, et al. Mol Cancer Ther 2003 Nov; 2(11): 1215-1222

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GSK3 beta antibody

Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using GSK3β antibody (AP02546PU-S or AP02546PU-N). Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 ml / €270.00
  Acris Antibodies GmbH

GSK3 beta antibody

Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using GSK3β antibody (AP02546PU-S or AP02546PU-N). Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
50 µl / €230.00
  Acris Antibodies GmbH

GSK3 beta antibody

Skeletal muscle, Human: Formalin-Fixed, Paraffin-Embedded (FFPE) Rabbit Purified Rt ICC/IF, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

GSK3 beta antibody

Human brain; Brain, cortex; GSK3B antibody - C-terminal region (ARP54644_P050) in Human brain cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Can, Hu, Ms, Por, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

GSK3 beta antibody

Western blot analysis of extracts from 293 cells treated with serum, using GSK3ß (Ab-9) Antibody antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GSK3 beta (4-12) pSer9 antibody

Anti-GSK3B / GSK3 Beta antibody IHC of human ovary. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml. Rabbit Aff - Purified Hu E, ICC/IF, IP, P, WB
50 µg / €345.00
  OriGene Technologies, Inc.

GSK3 beta pSer9 antibody

WB using Rabbit-anti-GSK3B pS9 antibody at a 1:1,000 dilution. All lanes contain human 293T whole cell lysate showing a band at 47 kDa. Cells were serum starved for 24 h prior to extraction. Key: Lane 1 Control, Lane 2 treated with IGF-1 (100 ng/ml) for 20', lane 3 pre-treated with 10 uM LY294002 (selective PI3K inhibitor) and treated with IGF-1 (100 ng/ml) for 20'. Molecular weight markers confirm a MW of ~ 49 kDa. Use a 1:2,000 dilution of HRP Goat-a-Rabbit IgG  for detection. Rabbit Aff - Purified Hu E, IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GSK3 beta antibody

Western blot analysis of Human small intestine stromal cancer tissue using TA323060 (GSK3B Antibody) at dilution 1/100 Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GSK3 beta antibody

Mouse Gsk3b Antibody (N-term) (Cat. #TA324570) western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).This demonstrates the Gsk3b antibody detected the Gsk3b protein (arrow). Rabbit IgG Purified Hu, Ms, Rt P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

GSK3 beta antibody

Western blot analysis of GSK3 beta expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GSK3 beta antibody

Western blot analysis of GSK-3Beta in a cell line mix, using a 1:1000 dilution of the antibody Rabbit Purified Bov, Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GSK3 beta antibody

Hela cell extract was resolved by electrophoresis, transferred to nitrocellulose and probed with rabbit anti-GSK 3ß antibody. Proteins were visualized using a donkey anti-rabbit secondary conjugated to HRP and a chemiluminescence detection system. Rabbit IgG Poly6042 Purified Hu, Ms WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

GSK3 beta antibody

Western Blot: GSK3 beta Antibody [TA336702] - Detection of GSK3B in A431 whole cell lysates using TA336702. Rabbit Purified Hu ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GSK3 beta antibody

Western Blot: GSK3 beta Antibody (3D10) [TA336772] - Western blot analysis using GSK3 beta mouse mAb against A549 (1), K562 (2), PC-12 (3), NIH/3T3 (4), and HEK293 (5) cell lysates. Mouse IgG2a 3D10 Purified Hu, Ms, Prim, Rt C, E, F, ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GSK3 beta (N-term) (incl. pos. control) antibody

GSK3 beta Mouse IgG1 11B9 Biotin Can, Hu, Ms, Rt E, WB
0.1 mg / €490.00
  Acris Antibodies GmbH

GSK3 beta (N-term) (incl. pos. control) antibody

Detection of Endogenous GSK3 beta: Whole cell lysates of serum starved tumor cells (20.000 cells per lane) were applied to SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with mab GSK3-11B9 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). 
Lane 1: A431
Lane 2:SW480 
Lane 3: SW620 
Lane 4: HT29 
Lane 5: MCF7 
Lane 6: MDA-MB-231 
Lane 7: T47D Mouse IgG1 11B9 Purified Can, Hu, Ms, Rt E, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

GSK3 beta pSer9 (incl. pos. control) antibody

GSK3 beta Mouse IgG1 3A8 Biotin Can, Hu, Ms E, WB
0.1 mg / €490.00
  Acris Antibodies GmbH

GSK3 beta pSer9 (incl. pos. control) antibody

Phosphospecificity Whole cell extracts of control (co), EGF stimulated (EGF) or  pervanadate treated (VH) A431 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to PVDF membranes. Immunoblots were probed with mab GSK-3A8 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Mouse IgG1 3A8 Purified Can, Hu, Ms E, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

GSK3 beta pSer9 (incl. pos. control) antibody

Phosphospecificity Whole cell extracts of control (co) and pervanadate (VH) treated A431 tumor cells were applied to SDS-PAGE (ca 20.000 cells per lane) and transferred to PVDF membranes. Immunoblots were probed with mab GSK3b-2D3 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). Mouse IgG1 2D3 Purified Hu, Ms E, WB
0.1 mg / €430.00
  Acris Antibodies GmbH

GSK3 beta antibody

GSK3 beta Mouse IgG2a SBGSK3a4 Purified Hu, Ms, Rt E, IP, WB
0.1 mg / €420.00
  Acris Antibodies GmbH

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