GST-Tag antibody

Principal name

GST-Tag antibody

Alternative names for GST-Tag antibody

Glutathione S-transferase Tag, GST26-Tag

SwissProt ID

P08515 (Schja)

Ncbi ID

6182

Available reactivities

All Species, Hu (Human), Ms (Mouse), Rt (Rat), Sj (Schistosoma japonicum), E. coli (Escherichia coli), Mam (Mammalia)

Available hosts

Rabbit, Mouse, Rat, Goat, Chicken

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Frozen Sections (C), Flow Cytometry (F), Dot blot (Dot), Paraffin Sections (P), ELISA (capture) (E(capture)), ELISA (detection) (E(detection))

Background of GST-Tag antibody

Epitope tagging is a powerful and versatile strategy for detecting and purifying proteins expressed by cloned genes. To utilize this feature, protein expression vectors are typically engineered with a nucleotide sequence that encodes the peptide epitope tag. The gene of interest is cloned in-frame relative to the tag and, upon expression, the protein of interest is synthesized as a fusion protein with the peptide tag. Fusion protein detection and/or purification is mediated by highly specific antibodies to the engineered peptide, thus eliminating the need for antibodies to proteins from each newly cloned gene. Commonly used epitope tags include glutathione-S-transferase (GST), c-myc, 6-histidine (6X-His), FLAG, green fluorescent protein (GFP), maltose binding protein (MBP), influenza A virus haemagglutinin (HA), b-galactosidase, and GAL4.

General readings

This product has been used in:
Lehti K et al. Oligomerization through hemopexin and cytoplasmic domains regulates the activity and turnover of membrane-type 1 matrix metalloproteinase. J Biol Chem 277:8440-8 (2002).
Chen, P-L., Chen, C-F., Chen, Y., Xiao, J., Sharp, Z.D. and Lee, W-H. (1998) The BRC repeats in BRCA2 are Critical for RAD51 Binding and Resistance to Methyl Methanesulfonate Treatment. Proc Natl Acad Science, USA 95:5287-5292. 1998
Carlos M. Luque, Carmen M. Pérez-Ferreiro, Alicia Pérez-González, Ludwig Englmeier, Maria D. Koffa, and Isabel Correas. An Alternative Domain Containing a Leucine-rich Sequence Regulates Nuclear Cytoplasmic Localization of Protein 4.1R. J. Biol. Chem., Jan 2003; 278: 2686 - 2691. 2003

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GST-Tag antibody

GST-Tag Goat IgG Aff - Purified Sj WB
20 µl / €50.00
  SICGEN

GST-Tag antibody

GST-Tag Goat IgG Aff - Purified Sj WB
1.2 mg / €320.00
  SICGEN

GST-Tag antibody

GST-Tag Goat IgG Aff - Purified Sj WB
3.0 mg / €450.00
  SICGEN

GST-Tag antibody

GST-Tag Goat IgG Aff - Purified Sj WB
20 µl / €50.00
  SICGEN

GST-Tag antibody

GST-Tag Goat IgG Aff - Purified Sj WB
0.6 mg / €300.00
  SICGEN

GST-Tag antibody

GST-Tag Goat IgG Aff - Purified Sj WB
1.5 mg / €420.00
  SICGEN

GST-Tag antibody

Bacterially expressed GST fusion protein immunoblot Mouse IgG1 N100/13 Supernatant E, WB
5 ml / €320.00
  Antibodies Incorporated

NeuroMab

GST-Tag antibody

Bacterially expressed GST fusion protein immunoblot Mouse IgG1 N100/13 Purified E, WB
0.1 ml / €380.00
  Antibodies Incorporated

NeuroMab

GST-Tag antibody

GST-Tag Goat IgG Aff - Purified Sj WB
1.2 mg / €320.00
  SICGEN

GST-Tag antibody

GST-Tag Goat IgG Aff - Purified Sj WB
3.0 mg / €450.00
  SICGEN

GST-Tag antibody

GST-Tag Goat IgG Aff - Purified Sj WB
0.6 mg / €300.00
  SICGEN

GST-Tag antibody

GST-Tag Goat IgG Aff - Purified Sj WB
1.5 mg / €420.00
  SICGEN

GST-Tag antibody

Antibody sensitivity Recombinant GST-PIN1 was separated by SDS-PAGE and transferred to PVDF membranes. Immunoblots were probed with mab 4H3 (0.5 µg/ ml) for 1h at RT and developed by ECL (exp. time: 30 sec). lane 1: 100ng, lane 2: 50ng, lane 3: 25ng, lane 4: 10ng, lane 5: 5ng, lane 6: 2ng, lane 7: 1ng Mouse IgG2a 4H3 Purified Sj E, IP, WB
0.1 mg / €380.00
  Acris Antibodies GmbH

GST-Tag antibody

Western Blot Mouse IgG1 KAB29 Purified WB
0.1 mg / €360.00
  Acris Antibodies GmbH

GST-Tag antibody

Western blot using Affinity Purified anti-GST antibody to detect purified GST recombinant protein (Left) and in a lysate of HeLa cells expressing recombinant GST protein (Right). Approximately 0.1 μg of protein was separated by SDS-PAGE and transferred onto nitrocellulose. After blocking the membrane was probed with the primary antibody diluted to 1.0 μg/ml for 1 h at RT followed by washes and reaction with a 1/5,000 dilution of IRDye(TM)800 conjugated Goat anti-Mouse IgG [H&L] MX. IRDye(TM)800 fluorescence image was captured using the Odyssey® Infrared Imaging System developed by LI-COR. IRDye is a trademark of LI-COR, Inc. Other detection systems will yield similar results. Mouse IgG1 3D4 Purified C, E, WB
0.5 mg / €420.00
  Acris Antibodies GmbH

GST-Tag antibody

Recombinant GST protein (20ng, Lane 1) and 293 cellular lysates transfected with a GST expression vector (20µg, Lane 2) were probed with anti-GST (1µg/ml). Arrow indicates GST (~26kDa) and GST-fustion protein (~29kDa). Mouse IgG2a GST.B6 Purified C, IP, WB
0.1 mg / €290.00
  Acris Antibodies GmbH

GST-Tag antibody

Figure 1. Western blot using GST Mab (Cat. #AM11012PU-N) to detect GST recombinant protein purified from bacterial lysate. Mouse IgG1 9AT106 Purified WB

Regular Price: 0.4 ml / €370.00

Special Price: 0.4 ml / €231.00

  Acris Antibodies GmbH

GST-Tag antibody

12G8 can specifically detect 1 ng of recombinant protein. There is 6 ng, 3 ng, 1 ng, and 0 ng GSTtagged recombinant protein in lanes 1-4, respectively. The 12G8 antibody dilution is 1/5000. Mouse IgG1 12G8 Aff - Purified All Species, Hu, Ms, Rt E, WB
0.2 ml / €350.00
  Acris Antibodies GmbH

GST-Tag antibody

12G8 can specifically detect 1 ng of recombinant protein. There is 6 ng, 3 ng, 1 ng, and 0 ng GSTtagged recombinant protein in lanes 1-4, respectively. The 12G8 antibody dilution is 1/5000. Mouse IgG1 12G8 Aff - Purified All Species, Hu, Ms, Rt E, WB
0.1 ml / €240.00
  Acris Antibodies GmbH

GST-Tag antibody

12G8 can specifically detect 1 ng of recombinant protein. There is 6 ng, 3 ng, 1 ng, and 0 ng GSTtagged recombinant protein in lanes 1-4, respectively. The 12G8 antibody dilution is 1/5000. Mouse IgG1 12G8 Aff - Purified All Species, Hu, Ms, Rt E, WB
50 µl / €190.00
  Acris Antibodies GmbH

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