GTF2B antibody

Principal name

GTF2B antibody

Alternative names for GTF2B antibody

Transcription initiation factor IIB, TF2B, TFIIB, General transcription factor TFIIB, S300-II

SwissProt ID

P62915 (Mouse), P62916 (Rat), Q00403 (Human), Q2KIN8 (Bovin)

Gene ID

2959 (GTF2B), 81673 (Gtf2b)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Rt (Rat), Ze (Zebrafish), African clawed frog, Mam (Mammalia), Mky (Monkey), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Electrophoretic Mobility Shift Assay (EMSA), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), PLA/Proximity ligation assay (PLA), Flow Cytometry (F)

Background of GTF2B antibody

GTF2B is the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II.This gene encodes the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Tubon,T.C., et al., (2004) Mol. Cell. Biol. 24 (7), 2863-2874

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GTF2B antibody

Western Blot analysis of GTF2B expression in transfected 293T cell line (H00002959-T02) by GTF2B MaxPab polyclonal antibody.

Lane 1: GTF2B transfected lysate(34.76 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu, Ms, Rt E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

GTF2B antibody

WB Suggested Anti-GTF2B Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human brain Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

GTF2B antibody

WB Suggested Anti-GTF2B Antibody Titration: 4.0ug/ml; Positive Control: HepG2 cell lysate Rabbit IgG Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GTF2B antibody

WB Suggested Anti-GTF2B Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human Placenta Rabbit IgG Purified Can, Eq, GP, Ms, Por, Rb, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

GTF2B antibody

Western blot detection of TFIIB in Jurkat,MCF7,COS7,3T3 and Hela cell lysates using TFIIB mouse mAb (1:500 diluted).Observed band size: 34KDa Mouse IgG2b 2F6-A3 Purified WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GTF2B antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GTF2B (RC204607, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GTF2B.(1:2000) Mouse IgG1 OTI1H10 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GTF2B antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GTF2B (RC204607, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GTF2B.(1:2000) Mouse IgG1 OTI2A7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GTF2B antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GTF2B (RC204607, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GTF2B.(1:2000) Mouse IgG1 OTI2A1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GTF2B antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GTF2B (RC204607, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GTF2B.(1:2000) Mouse IgG1 OTI2F5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GTF2B antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GTF2B (RC204607, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GTF2B.(1:2000) Mouse IgG2b OTI5B6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GTF2B antibody

Western blot analysis using GTF2B mouse mAb against Hela (1), NIH/3T3 (2), COS7 (3) and A431 (4) cell lysate. Mouse IgG1 4C1 Ascites Hu, Mky E, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

GTF2B antibody

Immunofluorescence of purified MaxPab antibody to GTF2B on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

GTF2B antibody

Immunofluorescence of purified MaxPab antibody to GTF2B on HeLa cell. [antibody concentration 10 ug/ml] Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

GTF2B antibody

GTF2B MaxPab rabbit polyclonal antibody. Western Blot analysis of GTF2B expression in mouse kidney. Rabbit Purified Hu, Ms E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

GTF2B (N-term) antibody

Rat Lung; WB Suggested Anti-Gtf2b Antibody. . Titration: 1.0 ug/ml. . Positive Control: Rat lung; Gtf2b antibody - N-terminal region (ARP58005_P050) in Rat Lung cells using Western Blot Rabbit Aff - Purified Hu, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

GTF2B antibody

Human Placenta; WB Suggested Anti-GTF2B Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:62500. Positive Control: Human Placenta; GTF2B antibody - C-terminal region (ARP58006_P050) in Human Placenta cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

GTF2B antibody

Human Brain; WB Suggested Anti-GTF2B Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Human brain; GTF2B antibody - N-terminal region (P100989_P050) in Human Brain cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Can, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

GTF2B antibody

Human Intestine; GTF2B antibody - N-terminal region (P100990_T100) in Human Intestine cells using Immunohistochemistry Rabbit Aff - Purified African clawed frog, Bov, Can, Hu, Ms, Rt, Ze P, WB
0.1 mg / €310.00
  AVIVA Systems Biology

GTF2B antibody

Immunohistochemistry: TFIIB Antibody [NBP1-32622] - analysis of paraffin-embedded Cal27 xenograft, using TFIIB  antibody at 1:100 dilution. Rabbit IgG Hu P, WB
0.1 mg / €420.00
  Novus Biologicals Inc.

GTF2B antibody

Western Blot: TFIIB Antibody [NBP1-49981] - Detection of Human GTF2B/ TFIIB by Western Blot of Immunoprecipitates.  Samples:  Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells.   Antibodies: Affinity purified rabbit anti- GTF2B/ TFIIB antibody used for IP at 3 mcg/mg lysate.  TFIIB was also immunoprecipitated by rabbit anti- GTF2B/ TFIIB antibody which recognizes a downstream epitope.  For blotting immunoprecipitated GTF2B/ TFIIB was used at 1 mcg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds. Rabbit Aff - Purified Hu IP, PLA
0.1 mg / €440.00
  Novus Biologicals Inc.

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