GTF2B antibody

Principal name

GTF2B antibody

Alternative names for GTF2B antibody

Transcription initiation factor IIB, TF2B, TFIIB, General transcription factor TFIIB, S300-II

SwissProt ID

P62915 (Mouse), P62916 (Rat), Q00403 (Human), Q2KIN8 (Bovin)

Gene ID

2959 (GTF2B), 81673 (Gtf2b), 229906

Available reactivities

Bov (Bovine), Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Mky (Monkey), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Mam (Mammalia)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Immunoprecipitation (IP)

Background of GTF2B antibody

GTF2B is the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II.This gene encodes the general transcription factor IIB, one of the ubiquitous factors required for transcription initiation by RNA polymerase II. The protein localizes to the nucleus where it forms a complex (the DAB complex) with transcription factors IID and IIA. Transcription factor IIB serves as a bridge between IID, the factor which initially recognizes the promoter sequence, and RNA polymerase II. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

General readings

Tubon,T.C., et al., (2004) Mol. Cell. Biol. 24 (7), 2863-2874

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GTF2B antibody

  Rabbit Polyclonal Anti-GTF2B Antibody  
WB Suggested Anti-GTF2B Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Human brain Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

GTF2B antibody

  Rabbit Polyclonal Anti-GTF2B Antibody  
WB Suggested Anti-GTF2B Antibody Titration: 4.0ug/ml; Positive Control: HepG2 cell lysate Rabbit IgG Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GTF2B antibody

  Rabbit Polyclonal Anti-GTF2B Antibody - C-terminal region  
WB Suggested Anti-GTF2B Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human Placenta Rabbit IgG Purified Can, Eq, GP, Ms, Por, Rb, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

GTF2B antibody

  Mouse Monoclonal TFIIB Antibody  
Western blot detection of TFIIB in Jurkat,MCF7,COS7,3T3 and Hela cell lysates using TFIIB mouse mAb (1:500 diluted).Observed band size: 34KDa Mouse IgG2b 2F6-A3 Purified WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GTF2B antibody

  Mouse Monoclonal anti-TFIIB Antibody  
GTF2B Mouse IgG2a IIB8 Purified Hu, Mam E, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GTF2B antibody

  GTF2B mouse monoclonal antibody, clone OTI1H10 (formerly 1H10)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GTF2B (RC204607, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GTF2B.(1:2000) Mouse IgG1 OTI1H10 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GTF2B antibody

  GTF2B mouse monoclonal antibody, clone OTI2A7 (formerly 2A7)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GTF2B (RC204607, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GTF2B.(1:2000) Mouse IgG1 OTI2A7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GTF2B antibody

  GTF2B mouse monoclonal antibody, clone OTI2A1 (formerly 2A1)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GTF2B (RC204607, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GTF2B.(1:2000) Mouse IgG1 OTI2A1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GTF2B antibody

  GTF2B mouse monoclonal antibody, clone OTI2F5 (formerly 2F5)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GTF2B (RC204607, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GTF2B.(1:2000) Mouse IgG1 OTI2F5 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GTF2B antibody

  GTF2B mouse monoclonal antibody, clone OTI5B6 (formerly 5B6)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY GTF2B (RC204607, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-GTF2B.(1:2000) Mouse IgG2b OTI5B6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GTF2B antibody

Western blot analysis using GTF2B mouse mAb against Hela (1), NIH/3T3 (2), COS7 (3) and A431 (4) cell lysate. Mouse IgG1 4C1 Ascites Hu, Mky E, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

GTF2B - human gene knockout kit via CRISPR

GTF2B - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

GTF2B - human gene knockout kit via CRISPR

GTF2B - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

GTF2B - human gene knockout kit via CRISPR

GTF2B - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

GTF2B - human gene knockout kit via CRISPR

GTF2B - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

GTF2B

  Recombinant protein of human general transcription factor IIB (GTF2B)  
GTF2B Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

GTF2B

  Recombinant protein of human general transcription factor IIB (GTF2B)  
GTF2B Human > 95 %
Preparation: .
Purity Detail: >95% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
10 µg / €399.00
  OriGene Technologies, Inc.

GTF2B (1-316, His-tag)

GTF2B Human Purified > 90 % by SDS - PAGE E. coli
0.5 mg / €670.00
  Acris Antibodies GmbH

GTF2B (1-316, His-tag)

GTF2B Human Purified > 90 % by SDS - PAGE E. coli
0.1 mg / €250.00
  Acris Antibodies GmbH

Lysates

Catalog No.    

GTF2B overexpression lysate

GTF2B overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

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