GTF2F1 / RAP74 antibody

Principal name

GTF2F1 / RAP74 antibody

Alternative names for GTF2F1 / RAP74 antibody

TFIIF-alpha, General transcription factor IIF subunit 1, Transcription initiation factor IIF subunit alpha, Transcription initiation factor RAP74, General transcription factor IIF polypeptide 1 74 kDa subunit protein

SwissProt ID

P35269 (Human), Q04870 (Xenla), Q3THK3 (Mouse), Q5EA53 (Bovin), Q6AY96 (Rat)

Gene ID

2962 (GTF2F1), 296229 (P22k15)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), African clawed frog, Can (Canine), Ze (Zebrafish), Mky (Monkey), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Chicken, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F)

Background of GTF2F1 / RAP74 antibody

The transcription factor IIF mediates the association of RNA polymerase II with promoter sequences containing transcription factors IID, IIB, and IIA (DAB complex). The resulting DNA-protein complex contained RNA polymerase II and the two subunits of transcription factor IIF (RAP 30 and RAP 74). Cloned human RAP 30 was sufficient for the recruitment of RNA polymerase II to the DAB complex. This ability of RAP 30 to recruit RNA polymerase to a promoter is also a characteristic of sigma factors in prokaryotes.

General readings

Zhang,C., et al., (2005) Mol. Cell. Biol. 25 (9), 3583-3595

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18 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GTF2F1 / RAP74 antibody

Anti-GTF2F1 mouse monoclonal antibody (TA503372) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GTF2F1(RC201294). Mouse IgG1 OTI1D5 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GTF2F1 / RAP74 antibody

Anti-GTF2F1 mouse monoclonal antibody (TA503373) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GTF2F1(RC201294). Mouse IgG1 OTI1C5 Purified Hu F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GTF2F1 / RAP74 antibody

Anti-GTF2F1 mouse monoclonal antibody (TA503380) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY GTF2F1(RC201294). Mouse IgG1 OTI2H3 Purified Hu, Mky, Ms F, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

GTF2F1 / RAP74 antibody

Immunohistochemical staining of paraffin-embedded Human endometrium tissue within the normal limits using anti-GTF2F1 mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA503604) Mouse IgG1 OTI4B10 Purified Can, Hu, Mky F, P, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

GTF2F1 / RAP74 antibody

WB Suggested Anti-GTF2F1 Antibody Titration: 0.2-1 ug/ml; Positive Control: K562 cell lysateThere is BioGPS gene expression data showing that GTF2F1 is expressed in K562 Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

GTF2F1 / RAP74 antibody

WB Suggested Anti-GTF2F1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1: 12500; Positive Control: HT1080 cell lysateGTF2F1 is strongly supported by BioGPS gene expression data to be expressed in Human HT1080 cells Rabbit IgG Purified Can, Eq, GP, Hu, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

GTF2F1 / RAP74 antibody

GTF2F1 / RAP74 Mouse Purified Hu E(detection), ICC/IF, WB
50 µg / €370.00
  Abnova Taiwan Corp.

GTF2F1 / RAP74 antibody

Human HT1080; WB Suggested Anti-GTF2F1 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: HT1080 cell lysate. GTF2F1 is strongly supported by BioGPS gene expression data to be expressed in Human HT1080 cells.; GTF2F1 antibody - N-terminal region (ARP38104_P050) in Human HT1080 cells using Western Blot Rabbit Aff - Purified African clawed frog, Bov, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

GTF2F1 / RAP74 antibody

Human K562; WB Suggested Anti-GTF2F1 Antibody Titration: 0.2-1 ug/ml. Positive Control: K562 cell lysate. There is BioGPS gene expression data showing that GTF2F1 is expressed in K562.; GTF2F1 antibody - N-terminal region (P100810_P050) in Human K562 cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt, Ze WB
50 µg / €350.00
  AVIVA Systems Biology

GTF2F1 / RAP74 antibody

Immunohistochemistry-Paraffin: RAP30 Antibody [NBP1-89922] - Immunohistochemical staining of human rectum shows strong nuclear positivity in glandular cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

GTF2F1 / RAP74 antibody

Immunohistochemistry-Paraffin: RAP30 Antibody [NBP1-90812] - staining of human rectum shows strong nuclear and cytoplasmic positivity in glandular cells. Rabbit Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

GTF2F1 / RAP74

GTF2F1 / RAP74 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells

Regular Price: 20 µg / €680.00

Special Price: 20 µg / €398.00

  OriGene Technologies, Inc.

GTF2F1 / RAP74

GTF2F1 / RAP74 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

GTF2F1 / RAP74

GTF2F1 / RAP74 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

GTF2F1 / RAP74

GTF2F1 / RAP74 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
10 µg / €380.00
  Abnova Taiwan Corp.

GTF2F1 / RAP74

GTF2F1 / RAP74 Human Purified 12.5% SDS-PAGE Stained with Coomassie Blue.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

GTF2F1 293T Cell Transient Overexpression Lysate(Denatured)

GTF2F1 293T Cell Transient Overexpression Lysate(Denatured)  
0.1 ml / €270.00
  Abnova Taiwan Corp.

GTF2F1 overexpression lysate

GTF2F1 overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

18 Item(s)

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