GTP cyclohydrolase 1 (GCH1) antibody

Principal name

GTP cyclohydrolase 1 (GCH1) antibody

Alternative names for GTP cyclohydrolase 1 (GCH1) antibody

GTP cyclohydrolase I, GTP-CH-I, DYT5, GCH

SwissProt ID

P30793 (Human), P50141 (Chick)

Gene ID

2643 (GCH1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit), Ye (Yeast), Ze (Zebrafish)

Available hosts

Mouse, Rabbit, Goat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Dot blot (Dot)

Background of GTP cyclohydrolase 1 (GCH1) antibody

Guanosine triphosphate cyclohydrolase I (GTPCHI) is the rate limiting enzyme for the synthesis of tetrahydrobioptrin, a cofactor for hydroxylation reaction in several pathways. GTPCHI is a critical enzyme in catecholamine function and is rate limiting for the synthesis of the catecholamine co-factor tetrahydrobiopterin (6BH)4, a key cofactor necessary for nitric oxide synthase and for the hydroxylases that are involved in the production of catecholamines and serotonin. (6R)-L-Erythro 5,6,7,8 tetrahydrobiopterin (6BH(4)) is crucial in the hydroxylation of L-phenylalanine-, L-tyrosine-, and L-tryptophan-regulating catecholamine and serotonin synthesis as well as tyrosinase in melanogenesis. The GTPCHI feed back regulatory protein (GFRP) binds to GTPCHI in presence of phenylalanine and inhibit its activity in the presence of biopterin. The shear stress in vasculature caused an up-regulation of eNOS and GTPCHI accompanied by increased blood flow. The GTPCHI increase caused a rise in BH4 production which required for sustained vasorelaxant response that increases the blood flow. The cofactor supply of (6BH)4 is indeed controlled by GFRP and GTPCHI in epidermal keratinocyltes and melanocytes for the biosynthesis of catecholamine and serotonin.
GTPCHI is a 241 aa (30 kDa) cytosolic protein that interact with GFPR. The expression of GTPCHI is reduced in nigrostraital neurons in middle and aged rats, monkeys and humans. The age related decrease in GTPHCI nigral neurons suggest a phenotypic-down regulation of this enzyme rather than generalized necrosis of cells and may be responsible for the generalized age-related decrease in dopaminergic tome with nigrostriatal system (1). Gene therapy approach for Parkinsonian condition was tested using purified adeno-associated virus containing tyrosine hydroxylase (TH) and GTPCHI to infect 6-OH denervated rat striatum. The viral delivery system indeed showed >90% of viral infected neurons to be TH positive (2), however, the production of L-DOPA was only evident in transfections with TH nd GTPCHI, suggesting that the trans-gene expression of TH and GTPCHI can be used to successfully deliver L-DOPA to striatum (2). The activity of GTPCHI is reduced by 50% in CNS of rats exhibiting chronic hepatic encephalopathy (3).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GTP cyclohydrolase 1 (GCH1) antibody

  Goat Anti-GCH1 Antibody  
TA305613 (1ug/ml) staining of Human Thymus lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

GTP cyclohydrolase 1 (GCH1) antibody

  Anti-GCH1 Rabbit Polyclonal Antibody  
Predicted band size: 28 kDa . Positive control: Human fetal liver tissue and Hela cell lysate. Recommended dilution: 1/200-1000 Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GTP cyclohydrolase 1 (GCH1) antibody

  Anti-GCH1 Rabbit Polyclonal Antibody  
Predicted band size: 24 kDa . Positive control: Human fetal liver tissue lysate. Recommended dilution: 1/500-2000 Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

GTP cyclohydrolase 1 (GCH1) antibody

  Rabbit Polyclonal Anti-GCH1 Antibody  
WB Suggested Anti-GCH1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: COLO205 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ye, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

GTP cyclohydrolase 1 (GCH1) antibody

  Rabbit Polyclonal Anti-GCH1 Antibody  
Host: Rabbit; Target Name: GCH1; Sample Tissue: Fetal Heart lysates; Antibody Dilution: 1.0 ug/ml Rabbit IgG Purified Bov, Can, Hu, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

CRISPR

Catalog No. Species Donor Vector  

GCH1 - human gene knockout kit via CRISPR

GCH1 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

GCH1 - human gene knockout kit via CRISPR

GCH1 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

GCH1 - human gene knockout kit via CRISPR

GCH1 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

GCH1 - human gene knockout kit via CRISPR

GCH1 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

GTP cyclohydrolase 1 (GCH1) (full length, N-term HIS tag)

  Recombinant protein of human GTP cyclohydrolase 1 (GCH1), full length, with N-terminal HIS tag, expressed in E.Coli, 50ug  
GTP cyclohydrolase 1 (GCH1) Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

GTP cyclohydrolase 1 (GCH1) (full length, N-term HIS tag, transcript variant 2)

  Purified recombinant protein of Human GTP cyclohydrolase 1 (GCH1), transcript variant 2, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug  
GTP cyclohydrolase 1 (GCH1) Human > 80 %
Preparation: .
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
E. coli
50 µg / €199.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, GCH1 (mGFP-tagged)-Human GTP cyclohydrolase 1 (GCH1), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, GCH1 (mGFP-tagged)-Human GTP cyclohydrolase 1 (GCH1), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, GCH1 (mGFP-tagged)-Human GTP cyclohydrolase 1 (GCH1), transcript variant 3, 200 uL, >10^7 TU/mL

Lenti ORF particles, GCH1 (mGFP-tagged)-Human GTP cyclohydrolase 1 (GCH1), transcript variant 3, 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, GCH1 (mGFP-tagged)-Human GTP cyclohydrolase 1 (GCH1), transcript variant 4, 200 uL, >10^7 TU/mL

Lenti ORF particles, GCH1 (mGFP-tagged)-Human GTP cyclohydrolase 1 (GCH1), transcript variant 4, 200 uL, >10^7 TU/mL  
200 µl / €880.00
  OriGene Technologies, Inc.

Lenti ORF particles, GCH1 (Myc-DDK-tagged)-Human GTP cyclohydrolase 1 (GCH1), transcript variant 2, 200 uL, >10^7 TU/mL

Lenti ORF particles, GCH1 (Myc-DDK-tagged)-Human GTP cyclohydrolase 1 (GCH1), transcript variant 2, 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

Lenti ORF particles, GCH1 (Myc-DDK-tagged)-Human GTP cyclohydrolase 1 (GCH1), transcript variant 3, 200 uL, >10^7 TU/mL

Lenti ORF particles, GCH1 (Myc-DDK-tagged)-Human GTP cyclohydrolase 1 (GCH1), transcript variant 3, 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

Lenti ORF particles, GCH1 (Myc-DDK-tagged)-Human GTP cyclohydrolase 1 (GCH1), transcript variant 4, 200 uL, >10^7 TU/mL

Lenti ORF particles, GCH1 (Myc-DDK-tagged)-Human GTP cyclohydrolase 1 (GCH1), transcript variant 4, 200 uL, >10^7 TU/mL  
200 µl / €780.00
  OriGene Technologies, Inc.

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