Guanylin antibody

Principal name

Guanylin antibody

Alternative names for Guanylin antibody

Guanylate cyclase activator 2A, GUCA2A, GUCA2, Guanylate cyclase-activating protein 1

SwissProt ID

P28902 (Rat), P33680 (Mouse), P70664 (Cavpo), P79897 (Pig), Q02747 (Human)

Gene ID

2980 (GUCA2A), 14915 (Guca2a), 25656

Available reactivities

Hu (Human), Rt (Rat)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Radioimmunoassay (R), Western blot / Immunoblot (WB), Immunoprecipitation (IP)

Background of Guanylin antibody

Heat-stable enterotoxins (STa) are small, cysteine-rich peptides secreted by Escherichia coli that are able to induce diarrhea through the stimulation of an intestine-specific receptor-guanylyl cyclase known as STaR. Binding of STa to STaR induces a dramatic increase in the cGMP content of the cell; the increase, in turn, inhibits salt absorption and stimulates chloride secretion. This imbalance of ions is accompanied by a massive accumulation of water in the gut that gives rise to the diarrhea and dehydration characteristic of enterotoxin activity. The identification of a receptor for STa on intestinal brush border membranes suggested the existence of an endogenous activator, described guanylin, a 15-amino acid peptide purified from rat small intestine, as a potential ligand for the STaR. This peptide shares sequence similarity with STa; see also uroguanylin. The molecular cloning of the human and mouse cDNAs encoding guanylin was reported. The sequences demonstrated that guanylin is present at the C-terminal end of a larger precursor protein. Expression in mammalian cells indicated that the 94-amino acid proguanylin is inactive. The biologically active guanylin can be released by either chemical or enzymatic treatment of proguanylin. By Northern blot analysis and in situ hybridization, showed that expression of guanylin mRNA is restricted to cells of the intestinal epithelium, specifically the Paneth cells at the base of the small intestinal crypts. These results demonstrate that guanylin is an endogenous activator of STaR isolated a cDNA encoding an apparent precursor of guanylin from a human intestinal cDNA library. The mRNA was expressed at high levels in human ileum and colon. In the mouse, interspecific backcross analysis used to map the Guca2 gene to the distal half of mouse chromosome 4 in a region of homology with human chromosome 1p. By fluorescence in situ hybridization mapped the GUCA2 gene to human 1p35-p34 Guanylin is thought to modulate intestinal water/electrolyte transport in a paracrine mode reported the nucleotide sequence of the gene, the characteristics of its circulating molecular form, and its localization in enterochromaffin cells of the gut. The gene, approximately 2.6 kb in size, consists of 3 exons interrupted by 2 introns. The hormonal form of guanylin is a 94-amino acid peptide with a molecular mass of 10.3 kDa. Guanylin is synthesized by gut enterochromaffin cells as a prohormone of 115 amino acids and is processed to the molecular form of 94 amino acids circulating in the blood.

General readings

Martin S, Adermann K, Forssmann WG, Kuhn M. Regulated, sidedirected secretion of proguanylin from isolated rat colonic mucosa. Endocrinology 1999;140:5022-9.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Guanylin (78-94) antibody

Guanylin Mouse IgG1 MAK-L-G-11 Purified Hu E
0.1 mg / €500.00
  Acris Antibodies GmbH

Guanylin (78-94) antibody

Guanylin Mouse IgG1 MAK-L-G-11 Purified Hu E
10 µg / €180.00
  Acris Antibodies GmbH

Guanylin (80-94) antibody

Guanylin Rabbit Serum Hu E
0.1 ml / €420.00
  Acris Antibodies GmbH

Guanylin (80-94) antibody

Guanylin Rabbit Serum Hu E
20 µl / €160.00
  Acris Antibodies GmbH

Guanylin (13-25) antibody

Guanylin Rabbit Serum Hu E, R, WB
0.1 ml / €420.00
  Acris Antibodies GmbH

Guanylin (13-25) antibody

Guanylin Rabbit Serum Hu E, R, WB
20 µl / €160.00
  Acris Antibodies GmbH

Guanylin (4-16) antibody

Guanylin Rabbit Serum Hu E, R, WB
0.1 ml / €420.00
  Acris Antibodies GmbH

Guanylin (4-16) antibody

Guanylin Rabbit Serum Hu E, R, WB
20 µl / €160.00
  Acris Antibodies GmbH

Guanylin (78-92) antibody

Guanylin Rabbit Serum Rt E, R
0.1 ml / €480.00
  Acris Antibodies GmbH

Guanylin (78-92) antibody

Guanylin Rabbit Serum Rt E, R
20 µl / €170.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

GUCA2A - human gene knockout kit via CRISPR

GUCA2A - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

GUCA2A - human gene knockout kit via CRISPR

GUCA2A - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

GUCA2A - human gene knockout kit via CRISPR

GUCA2A - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

GUCA2A - human gene knockout kit via CRISPR

GUCA2A - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Guca2a - mouse gene knockout kit via CRISPR

Guca2a - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Guca2a - mouse gene knockout kit via CRISPR

Guca2a - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Guca2a - mouse gene knockout kit via CRISPR

Guca2a - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Guca2a - mouse gene knockout kit via CRISPR

Guca2a - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Guanylin

  Recombinant protein of human guanylate cyclase activator 2A (guanylin) (GUCA2A)  
Guanylin Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Guanylin

12% SDS-PAGE separation of Human Proguanylin 1. M.W. marker - 14, 21, 31, 45, 66, 97 kDa 2. reduced and heated sample, 5µg/lane 3. non-reduced and non-heated sample, 5µg/lane Human Purified > 95 % >95% E. coli
0.1 mg / €470.00
  Acris Antibodies GmbH

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