GUCY2D / RETGC1 antibody

Principal name

GUCY2D / RETGC1 antibody

Alternative names for GUCY2D / RETGC1 antibody

Retinal guanylyl cyclase 1, RETGC-1, retinal Guanylate cyclase 2D, CORD6, GUC1A4, GUC2D, RETGC, PGCD, Rod outer segment membrane guanylate cyclase

SwissProt ID

O19179 (Canfa), P51839 (Rat), P55203 (Bovin), Q02846 (Human)

Gene ID

3000 (GUCY2D)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Flow Cytometry (F), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of GUCY2D / RETGC1 antibody

Cyclic GMP (cGMP), a key messenger in several signal transduction pathways, the intracellular levels of cGMP ate maintained by the activity of opposing enzymes: synthesizing gualylyl cyclases (GC) and hydrolyzing phosphodiesterases (PDEs). The synthesizing enzymes (GCs) are found in two forms: cytosolic (soluble) and membrane-bound (particulate), while they share similar structural characteristics, they differ in their mechanisms of physiological regulations. Most importantly, sGC contains a heme group and binds NO that activates the enzyme, while particulate GC is stimulated by natriuretic peptides. Membrane-bound guanylyl cyclases (GCs) are peptide hormone receptors whereas the cytosolic isoforms are receptors for nitric oxide. Particulate GC (PGCs) have 7 different isoforms, PGCA through PGCG and are expressed in most tissues in isoform specific manner. Plasma membrane forms of guanylyl cyclase have been shown to function as natriuretic peptide receptors. In response to G-protein couples receptor stimulation, the cGMP can be produced from GTP by either cytoplasmic, soluble guanylate cyclase (sGC) are heterodimers, that are stimulated by nitric oxide and carbon monoxide or by particulate membrane-bound guanylyl cyclases which are activated by a complex mechanism by natriuretic peptides. There is significant structural homology among various PGCs, there is a large N-terminal extracellular domain (ECD), a single TMD and a large intracellular domain with protein kinase activity (KLD), a C terminal catalytic domain (CD) and in between is a dimmerization domain (DD). Two cDNA clones isolated from rat eye cDNA library encodes for two membranes associated guanylate cyclase E and F. The expression of PGCF is confined to eye, none of the ligand kown to stimulate other guanylate cyclases failed to stimulate PGCE and F subtype. Tus both PGCE and PGCF are considered as orphan receptors. The PGCF structure resembles most closely with other sensory PGC isoforms, the conservations are in the intracellular kinase like and catalytic domains, and is most divergent at N and C terminal regions. The PGCF is expressed only in retina. It is suggested that membrane receptor GCs may be involved in the control of inner ear electrolyte and fluid composition whereas NO stimulated GC isoforms mainly participate in the regulation of blood flow and supporting cell physiology. At present PGC-E and PGCF ligands are not known and they fall under Orphan Receptor category.

General readings

Silva, L.K., et al. Eur. J. Hum. Genet. 18(11):1221-1227(2010)
Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010)
Joslyn, G., et al. Alcohol. Clin. Exp. Res. 34(5):800-812(2010)
Pasadhika, S., et al. Invest. Ophthalmol. Vis. Sci. 51(5):2608-2614(2010)
Sundaresan, P., et al. Mol. Vis. 15, 1781-1787 (2009)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GUCY2D / RETGC1 (C-term) antibody

GUCY2D / RETGC1 Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €420.00
  Acris Antibodies GmbH

GUCY2D / RETGC1 (Center) antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human thyroid tissue reacted with GUCY2D / GUCY2D / RETGC1 Antibody (Center) Cat..-No AP51984PU-N, which was peroxidase conjugated to the secondary antibody and followed by DAB staining. Rabbit Ig Aff - Purified Hu F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

GUCY2D / RETGC1 antibody

GUCY2D / RETGC1 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

GUCY2D / RETGC1 antibody

GUCY2D / RETGC1 Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

GUCY2D / RETGC1 antibody

GUCY2D monoclonal antibody (M01), clone 4E12. Western Blot analysis of GUCY2D expression in human spleen. Mouse IgG 4E12 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

GUCY2D / RETGC1 antibody

Detection limit for recombinant GST tagged GUCY2D is approximately 0.1ng/ml as a capture antibody. Mouse IgG 6D9 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

GUCY2D / RETGC1 antibody

Detection limit for recombinant GST tagged GUCY2D is approximately 0.03ng/ml as a capture antibody. Mouse IgG 6E9 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

GUCY2D / RETGC1 antibody

GUCY2D / RETGC1 Mouse IgG 7B6 Ascites Hu E, WB
0.2 ml / €370.00
  Abnova Taiwan Corp.

GUCY2D / RETGC1 antibody

Detection limit for recombinant GST tagged GUCY2D is approximately 0.3ng/ml as a capture antibody. Mouse IgG 5E10 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

GUCY2D / RETGC1 antibody

Detection limit for recombinant GST tagged GUCY2D is approximately 0.1ng/ml as a capture antibody. Mouse IgG 1E6 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

GUCY2D / RETGC1 antibody

Detection limit for recombinant GST tagged GUCY2D is approximately 0.1ng/ml as a capture antibody. Mouse IgG 7E5 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

GUCY2D / RETGC1 antibody

Detection limit for recombinant GST tagged GUCY2D is approximately 0.1ng/ml as a capture antibody. Mouse IgG 1F12 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

GUCY2D / RETGC1 antibody

GUCY2D / RETGC1 Mouse IgG1 X1 Purified Hu E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

GUCY2D / RETGC1

GUCY2D / RETGC1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

GUCY2D / RETGC1

GUCY2D / RETGC1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
10 µg / €380.00
  Abnova Taiwan Corp.

GUCY2D / RETGC1

GUCY2D / RETGC1 Human 12.5% SDS-PAGE Stained with Coomassie Blue. in vitro transl.
25 µg / €510.00
  Abnova Taiwan Corp.

Lysates

Catalog No.    

GUCY2D overexpression lysate

GUCY2D overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

17 Item(s)

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