GUCY2D / RETGC1 antibody

Principal name

GUCY2D / RETGC1 antibody

Alternative names for GUCY2D / RETGC1 antibody

Retinal guanylyl cyclase 1, RETGC-1, retinal Guanylate cyclase 2D, CORD6, GUC1A4, GUC2D, RETGC, PGCD, Rod outer segment membrane guanylate cyclase

SwissProt ID

O19179 (Canfa), P51839 (Rat), P55203 (Bovin), Q02846 (Human)

Gene ID

3000 (GUCY2D), 113911 (Gucy2d)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Flow Cytometry (F), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of GUCY2D / RETGC1 antibody

Cyclic GMP (cGMP), a key messenger in several signal transduction pathways, the intracellular levels of cGMP ate maintained by the activity of opposing enzymes: synthesizing gualylyl cyclases (GC) and hydrolyzing phosphodiesterases (PDEs). The synthesizing enzymes (GCs) are found in two forms: cytosolic (soluble) and membrane-bound (particulate), while they share similar structural characteristics, they differ in their mechanisms of physiological regulations. Most importantly, sGC contains a heme group and binds NO that activates the enzyme, while particulate GC is stimulated by natriuretic peptides. Plasma membrane forms of guanylyl cyclase have been shown to function as natriuretic peptide receptors. In response to G-protein couples receptor stimulation, the cGMP can be produced from GTP by either cytoplasmic, soluble guanylate cyclase (sGC) are heterodimers (a & b polypeptide chains), that are stimulated by nitric oxide and carbon monoxide or by particulate membrane-bound guanylyl cyclases which are activated by a complex mechanism by natriuretic peptides. Particulate GC (PGCs) have 7 different isoforms, PGC-A through PGC-G and are expressed in most tissues in isoform specific manner (See Table 1). There is significant structural homology among various PGCs, there is a large N-terminal extracellular domain (ECD), a single TMD and a large intracellular domain with protein kinase activity (KLD), a C-terminal catalytic domain (CD) and in between is a dimmerization domain (DD). The particulate GC-D, also known as olfactory gunylate cyclas, is specifically expressed in a subpopulation of olfactory sensory neurons. The expression of GC-D RNA is restricted to a small, randomly dispersed population of neurons that is within a single topographic zone in the olfactory neuroepithelium and resembles the pattern of the more diverse seven-transmembrane-domain odorant receptors (1). Particulate GC-D resembles two other GC expressed in retina (P-GC-E and F), but diverges considerable form other GCs. These observations suggest that GC-D may function directly in odor recognition or in modulating the sensitivity of a subpopulation of sensory neurons to specific odors.

General readings

Silva, L.K., et al. Eur. J. Hum. Genet. 18(11):1221-1227(2010)
Liu, C.Y., et al. Carcinogenesis 31(7):1259-1263(2010)
Joslyn, G., et al. Alcohol. Clin. Exp. Res. 34(5):800-812(2010)
Pasadhika, S., et al. Invest. Ophthalmol. Vis. Sci. 51(5):2608-2614(2010)
Sundaresan, P., et al. Mol. Vis. 15, 1781-1787 (2009)

11 Item(s)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

GUCY2D / RETGC1 (Center) antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human thyroid tissue reacted with GUCY2D / GUCY2D / RETGC1 Antibody (Center) Cat..-No AP51984PU-N, which was peroxidase conjugated to the secondary antibody and followed by DAB staining. Rabbit Ig Aff - Purified Hu F, P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

GUCY2D - human gene knockout kit via CRISPR

GUCY2D - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

GUCY2D - human gene knockout kit via CRISPR

GUCY2D - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

GUCY2D - human gene knockout kit via CRISPR

GUCY2D - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

GUCY2D - human gene knockout kit via CRISPR

GUCY2D - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

GUCY2D / RETGC1

  Recombinant protein of human guanylate cyclase 2D, membrane (retina-specific) (GUCY2D)  
GUCY2D / RETGC1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

GUCY2D overexpression lysate

GUCY2D overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, GUCY2D (mGFP-tagged) - Human guanylate cyclase 2D, membrane (retina-specific) (GUCY2D), 200 uL, >10^7 TU/mL

Lenti ORF particles, GUCY2D (mGFP-tagged) - Human guanylate cyclase 2D, membrane (retina-specific) (GUCY2D), 200 uL, >10^7 TU/mL  
200 µl / €2,210.00
  OriGene Technologies, Inc.

Lenti ORF particles, GUCY2D (Myc-DDK tagged) - Human guanylate cyclase 2D, membrane (retina-specific) (GUCY2D) , 200 uL, >10^7 TU/mL

Lenti ORF particles, GUCY2D (Myc-DDK tagged) - Human guanylate cyclase 2D, membrane (retina-specific) (GUCY2D) , 200 uL, >10^7 TU/mL  
200 µl / €1,990.00
  OriGene Technologies, Inc.

Rat Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Gucy2d (GFP-tagged ORF) - Rat guanylate cyclase 2d (Gucy2d), 200 uL, >10^7 TU/mL

Lenti ORF particles, Gucy2d (GFP-tagged ORF) - Rat guanylate cyclase 2d (Gucy2d), 200 uL, >10^7 TU/mL  
200 µl / €2,290.00
  OriGene Technologies, Inc.

Lenti ORF particles, Gucy2d (Myc-DDK-tagged ORF) - Rat guanylate cyclase 2d (Gucy2d), 200 uL, >10^7 TU/mL

Lenti ORF particles, Gucy2d (Myc-DDK-tagged ORF) - Rat guanylate cyclase 2d (Gucy2d), 200 uL, >10^7 TU/mL  
200 µl / €2,067.00
  OriGene Technologies, Inc.

11 Item(s)

per page
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