H2AFY / MACROH2A1 antibody

Principal name

H2AFY / MACROH2A1 antibody

Alternative names for H2AFY / MACROH2A1 antibody

Core histone macro-H2A.1, Histone macroH2A1, mH2A1, H2A.y, H2A/y, MU-MB-50.205, Medulloblastoma antigen MU-MB-50.205

SwissProt ID

O75367 (Human), O93327 (Chick), Q02874 (Rat), Q9QZQ8 (Mouse)

Gene ID

9555 (H2AFY), 26914 (H2afy), 29384

Available reactivities

Bov (Bovine), Can (Canine), Chk (Chicken), Hu (Human), Ms (Mouse), Rt (Rat), Ze (Zebrafish), African clawed frog, Eq (Equine), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit

Available applications

Western blot / Immunoblot (WB)

Background of H2AFY / MACROH2A1 antibody

Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. H2AFY is a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and participates in stable X chromosome inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms.Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and participates in stable X chromosome inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms.

General readings

Bernstein,E., (2008) Proc. Natl. Acad. Sci. U.S.A. 105 (5), 1533-1538

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

H2AFY / MACROH2A1 antibody

  Rabbit Polyclonal Anti-H2AFY Antibody - N-terminal region  
WB Suggested Anti-H2AFY Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: MCF7 cell lysate Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt, Ze WB
50 µg / €325.00
  OriGene Technologies, Inc.

H2AFY / MACROH2A1 antibody

  Rabbit Polyclonal Anti-H2AFY Antibody - middle region  
WB Suggested Anti-H2AFY Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: 293T cell lysateH2AFY is strongly supported by BioGPS gene expression data to be expressed in Human HEK293T cells Rabbit IgG Purified Bov, Can, Eq, Ms, Por, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

H2AFY / MACROH2A1 antibody

  Rabbit Polyclonal Anti-H2AFY Antibody - N-terminal region  
WB Suggested Anti-H2AFY Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: NCI-H226 cell lysateH2AFY is strongly supported by BioGPS gene expression data to be expressed in NCI-H226 Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

CRISPR

Catalog No. Species Donor Vector  

H2AFY - human gene knockout kit via CRISPR

H2AFY - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

H2AFY - human gene knockout kit via CRISPR

H2AFY - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

H2AFY - human gene knockout kit via CRISPR

H2AFY - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

H2AFY - human gene knockout kit via CRISPR

H2AFY - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

H2afy - mouse gene knockout kit via CRISPR

H2afy - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

H2afy - mouse gene knockout kit via CRISPR

H2afy - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

H2afy - mouse gene knockout kit via CRISPR

H2afy - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

H2afy - mouse gene knockout kit via CRISPR

H2afy - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

H2AFY / MACROH2A1 (transcript variant 2)

  Recombinant protein of human H2A histone family, member Y (H2AFY), transcript variant 2  
H2AFY / MACROH2A1 Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

H2AFY overexpression lysate

H2AFY overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

H2AFY overexpression lysate

H2AFY overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

H2AFY overexpression lysate

H2AFY overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, H2AFY (mGFP-tagged)-Human H2A histone family, member Y (H2AFY), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, H2AFY (mGFP-tagged)-Human H2A histone family, member Y (H2AFY), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €950.00
  OriGene Technologies, Inc.

Lenti ORF particles, H2AFY (mGFP-tagged)-Human H2A histone family, member Y (H2AFY), transcript variant 3, 200 uL, >10^7 TU/mL

Lenti ORF particles, H2AFY (mGFP-tagged)-Human H2A histone family, member Y (H2AFY), transcript variant 3, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, H2AFY (mGFP-tagged) - Human H2A histone family, member Y (H2AFY), transcript variant 4, 200 uL, >10^7 TU/mL

Lenti ORF particles, H2AFY (mGFP-tagged) - Human H2A histone family, member Y (H2AFY), transcript variant 4, 200 uL, >10^7 TU/mL  
200 µl / €1,050.00
  OriGene Technologies, Inc.

Lenti ORF particles, H2AFY (Myc-DDK-tagged)-Human H2A histone family, member Y (H2AFY), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, H2AFY (Myc-DDK-tagged)-Human H2A histone family, member Y (H2AFY), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €850.00
  OriGene Technologies, Inc.

Lenti ORF particles, H2AFY (Myc-DDK-tagged)-Human H2A histone family, member Y (H2AFY), transcript variant 3, 200 uL, >10^7 TU/mL

Lenti ORF particles, H2AFY (Myc-DDK-tagged)-Human H2A histone family, member Y (H2AFY), transcript variant 3, 200 uL, >10^7 TU/mL  
200 µl / €940.00
  OriGene Technologies, Inc.

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