HAT1 antibody

Principal name

HAT1 antibody

Alternative names for HAT1 antibody

Histone acetyltransferase type B catalytic subunit

SwissProt ID

O14929 (Human), Q12341 (Yeast), Q5M939 (Rat), Q8BY71 (Mouse)

Gene ID

8520 (HAT1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Mky (Monkey), Por (Porcine)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P)

Background of HAT1 antibody

The basic repeating unit of eukaryotic chromatin is the nucleosome, which consists of 147 bp of DNA wrapped around an octameric protein core formed of two molecules each of histones H2A, H2B, H3, and H4. These core histones are required for nuclear packaging and DNA organization as well as for regulating various cell processes. Acetylation is the most extensively studied posttranslational histone modification. Histone acetyltransferases (HATs) are members of a superfamily of enzymes that transfer the acetyl moiety from acetyl-coenzyme A cofactor onto one or more epsilon-amino groups of lysines contained in the extended N-terminal tail domains of core histone proteins. Type A HATs are located in the nucleus, and many of them play a role as transcriptional coactivators. Type B HATs, traditionally thought to locate to the cytoplasm, acetylate nascent cytoplasmic histones prior to chromatin assembly. HAT1, a type B HAT, has been identified in several species ranging from yeast and maize to human. Native human HAT1 is a ~100 kDa heterodimer consisting of a catalytic subunit (46 kDa) and a corehistone- binding subunit (Rbap46/Hat2) that greatly stimulates the acetyltransferase activity of the holoenzyme. Human HAT1 rapidly diacetylates soluble non-nucleosomal histone H4 at lysine-5 and lysine-12 with similar efficiency. It also acetylates, albeit to a lesser extent, histone H2A at lysine 5. These sites are distinct from those modified in transcriptional regulation. The newly synthesized acetylated histones interact with cellular proteins such as chromatin assembly factor 1 (CAF-1) to form complexes. Following deposition of the acetylated histones into chromosomes they are rapidly deaceylated. HAT1 is present in S-phase cell nuclei and in the cytoplasm. HAT1 may be involved in processes of chromatin assembly, chromatin repair, maintenance of specific heterochromatin acetylation pattern, and telomeric silencing.

General readings

Marmorstein R., et al. (2001) J Mol Biol. 311(3):433-44.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

HAT1 antibody

Western blot analysis of extracts from LOVO cells, using HAT antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HAT1 antibody

Western blot analysis of extracts of various cell lines, using HAT1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

HAT1 antibody

Western blot analysis on LOVO cell lysate using HAT Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HAT1 (N-term) antibody

Western blot analysis of KAT1 (HAT1) Antibody (N-term) Cat.-No AP11103PU-N in HL60 cell line lysates (35ug/lane). HAT1 (arrow) was detected using the purified Pab. Rabbit Ig Purified Hu WB

Regular Price: 0.4 ml / €370.00

Special Price: 0.4 ml / €231.00

  Acris Antibodies GmbH

HAT1 (C-term) antibody

Western blot analysis of HAT1 Antibody (C-term) Cat.-No AP11104PU-N in 293, HepG2, Jurkat cell line lysates (35ug/lane). HAT1 (arrow) was detected using the purified Pab. Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

HAT1 antibody

Western blot analysis of HAT1 antibody (AP20234PU-N) in extracts from LOVO cells. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 mg / €350.00
  Acris Antibodies GmbH

HAT1 antibody

HAT1 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

HAT1 antibody

HAT1 Rabbit Hu E
0.4 ml / €390.00
  Abnova Taiwan Corp.

HAT1 antibody

HAT1 Rabbit Hu E
0.4 ml / €390.00
  Abnova Taiwan Corp.

HAT1 antibody

Western blot analysis of HAT1 expression in Jurkat cell lysate. The antibody recognizes 50 kDa HAT-1 from samples of human, mouse and rat origins. Rabbit Hu, Ms, Rt WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

HAT1 antibody

Western blot analysis of extracts from K562 cells, using HAT Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
50 µg / €205.00
  Assay Biotech

HAT1 antibody

Western blot analysis of extracts from K562 cells, using HAT Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €310.00
  Assay Biotech

HAT1 antibody

Western blot analysis of HAT1 expression in HeLa (A); BT20 (B); A431 (C); HEK293T (D) whole cell lysates. Rabbit Aff - Purified Bov, Can, Hu, Mky, Por WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

HAT1 antibody

Western blot analysis of HAT1 expression in HeLa (A); BT20 (B); A431 (C); HEK293T (D) whole cell lysates. Rabbit Aff - Purified Bov, Can, Hu, Mky, Por WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

HAT1 antibody

Western blot analysis of HAT1 expression in HeLa (A); BT20 (B); A431 (C); HEK293T (D) whole cell lysates. Rabbit Aff - Purified Bov, Can, Hu, Mky, Por WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

HAT1 antibody

HAT1 Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €360.00
  Novus Biologicals Inc.

HAT1 antibody

HAT1 Rabbit IgG Aff - Purified Hu, Ms, Rt E, WB
50 µg / €290.00
  Novus Biologicals Inc.

HAT1 antibody

Immunohistochemistry-Paraffin: KAT1 Antibody [NBP1-87835] - Immunohistochemical staining of human colon shows strong nuclear positivity in glandular cells. Rabbit IgG Aff - Purified Hu ICC/IF, P, WB
0.1 ml / €500.00
  Novus Biologicals Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

HAT1 (20-341, His-tag)

HAT1 Human Purified > 90 % by SDS – PAGE E. coli
0.25 mg / €1,000.00
  Acris Antibodies GmbH

HAT1 (20-341, His-tag)

HAT1 Human Purified > 90 % by SDS – PAGE E. coli
50 µg / €370.00
  Acris Antibodies GmbH

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