HCK antibody

Principal name

HCK antibody

Alternative names for HCK antibody

Hemopoietic cell kinase, p59-HCK, p60-HCK, BMK, p56-HCK, Tyrosine-protein kinase HCK, B-cell/myeloid kinase

SwissProt ID

P08103 (Mouse), P08631 (Human), P50545 (Rat), Q9H5Y5 (Human)

Gene ID

3055 (HCK)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), EPair for Elisa (EPair), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Flow Cytometry (F), Frozen Sections (C)

Background of HCK antibody

Hck (hemopoietic cell kise) is a protein tyrosine kise of the Src family prominently expressed in the lymphoid and myeloid lineages of hemopoiesis. It participates in transducing a variety of extracellular sigls, which ultimately affect cellular processes including proliferation, differentiation and migration.The well-defined modular structure of Hck comprises a relatively divergent, NH2-termil "unique" domain, which is subject to post-translatiol lipid modifications thereby targeting Hck to the plasma membrane. Src homology 3 (SH3) and 2 (SH2) domains, and a tyrosine kise catalytic domain follow the "unique" domain. The catalytic activity of Hck is regulated, both positively and negatively, by tyrosine phosphorylation of highly conserved tyrosine (Y) residues. Phosphorylation of a single conserved Tyr499 residue in the COOH terminus of Hck by the protein kise Csk renders Hck ictive as a result of an intramolecular interaction between the phosphorylated tyrosine (pY) residue and its own SH2 domain. Disruption of this interaction, either as a result of dephosphorylation, or substitution of the COOH-termil regulatory Y residue with phenylalanine (F; e.g., HckY499F), or COOH-termil truncation mutations as observed in the virally transduced v-Src oncoprotein, results in constitutive activation of Hck. In contrast to phosphorylation of the COOH-termil regulatory tyrosine residue, autophosphorylation of a tyrosine residue (Tyr388) within the kise domain of Hck acts to positively regulate its catalytic activity. Thus, activation of Hck requires both disruption of the COOH-termil regulatory tyrosine-SH2 domain interaction and autophosphorylation of the regulatory tyrosine residue within the kise domain. The dysfunction or dysregulation of Hck may contribute to the pathogenesis of some human leukemias.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

HCK antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between HCK and CRKL Mahlavu cells were stained with anti-HCK rabbit purified polyclonal 1:1200 and anti-CRKL mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

HCK antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue using HCK antibody.The picture on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, ICC/IF, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

HCK antibody

HCK Antibody (N-term) (Cat. #TA324975) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the HCK antibody detected the HCK protein (arrow). Rabbit IgG Purified Hu, Mky, Ms, Rt F, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

HCK antibody

Western blot analysis of extracts of HL-60 cell lines, using HCK antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HCK antibody

WB Suggested Anti-HCK Antibody; Titration: 1.0 ug/ml; Positive Control: Fetal Thymus Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

HCK antibody

Immunohistochemical analysis of paraffin-embedded human colon cancer (left) and ancreas cancer (right), showing cytoplasmic localization using HCK antibody Cat.-No  AM06200SU-N with DAB staining. Mouse IgG1 3D12E10 Ascites Hu P, WB
0.1 ml / €440.00
  Acris Antibodies GmbH

HCK (N-term) antibody

HCK Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

HCK pTyr521 antibody

Western blot analysis of extracts from JurKat cells using HCK (Phospho-Tyr521) Antibody AP55839PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 ml / €340.00
  Acris Antibodies GmbH

HCK pTyr521 antibody

Western blot analysis of extracts from JurKat cells using HCK (Phospho-Tyr521) Antibody AP55839PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µl / €270.00
  Acris Antibodies GmbH

HCK antibody

Western Blot analysis of HCK expression in transfected 293T cell line (H00003055-T01) by HCK MaxPab polyclonal antibody.

Lane 1: HCK transfected lysate(55.55 KDa).
Lane 2: Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

HCK antibody

Western Blot analysis of HCK expression in transfected 293T cell line (H00003055-T02) by HCK MaxPab polyclonal antibody.

Lane 1: HCK transfected lysate(55.55 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

HCK antibody

HCK Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

HCK antibody

HCK Mouse IgG1 1D9-1A9 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

HCK antibody

Proximity Ligation Analysis (PLA) of protein-protein interactions between FLT1 and HCK Huh7 cells were stained with anti-FLT1 rabbit purified polyclonal 1:1200 and anti-HCK mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613(red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex. Mouse IgG2a 2A6 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

HCK pTyr410 antibody

Immunofluorescence analysis of HeLa cells, using HCK (Phospho-Tyr410) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF
50 µg / €205.00
  Assay Biotech

HCK pTyr410 antibody

Immunofluorescence analysis of HeLa cells, using HCK (Phospho-Tyr410) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF
0.1 mg / €310.00
  Assay Biotech

HCK antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using HCK (Ab-410) Antibody Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF
50 µg / €205.00
  Assay Biotech

HCK antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using HCK (Ab-410) Antibody Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF
0.1 mg / €310.00
  Assay Biotech

HCK antibody

Western Blot: Hck Antibody (EP2415Y) [NB100-79963] - Western blot analysis on TF-1 cell lysate using anti-Hck Antibody, dilution 1:200,000. Rabbit IgG EP2415Y Hu F, ICC/IF, IP, WB
0.1 ml / €480.00
  Novus Biologicals Inc.

HCK antibody

Immunohistochemistry: HCK Antibody (3D12E10) [NBP1-47514] - Immunohistochemical analysis of paraffin-embedded human colon cancer(left) and ancreas cancer(right), showing cytoplasmic localization using anti-HCK antibody with DAB staining. Mouse IgG1 3D12E10 Purified Hu C, E, P, WB
0.1 ml / €440.00
  Novus Biologicals Inc.

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