HCK antibody

Principal name

HCK antibody

Alternative names for HCK antibody

Hemopoietic cell kinase, p59-HCK, p60-HCK, BMK, p56-HCK, Tyrosine-protein kinase HCK, B-cell/myeloid kinase

SwissProt ID

P08103 (Mouse), P08631 (Human), P50545 (Rat), Q9H5Y5 (Human)

Gene ID

3055 (HCK), 15162 (Hck)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey), Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), EPair for Elisa (EPair), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P), Flow Cytometry (F)

Background of HCK antibody

Hck (hemopoietic cell kise) is a protein tyrosine kise of the Src family prominently expressed in the lymphoid and myeloid lineages of hemopoiesis. It participates in transducing a variety of extracellular sigls, which ultimately affect cellular processes including proliferation, differentiation and migration.The well-defined modular structure of Hck comprises a relatively divergent, NH2-termil "unique" domain, which is subject to post-translatiol lipid modifications thereby targeting Hck to the plasma membrane. Src homology 3 (SH3) and 2 (SH2) domains, and a tyrosine kise catalytic domain follow the "unique" domain. The catalytic activity of Hck is regulated, both positively and negatively, by tyrosine phosphorylation of highly conserved tyrosine (Y) residues. Phosphorylation of a single conserved Tyr499 residue in the COOH terminus of Hck by the protein kise Csk renders Hck ictive as a result of an intramolecular interaction between the phosphorylated tyrosine (pY) residue and its own SH2 domain. Disruption of this interaction, either as a result of dephosphorylation, or substitution of the COOH-termil regulatory Y residue with phenylalanine (F; e.g., HckY499F), or COOH-termil truncation mutations as observed in the virally transduced v-Src oncoprotein, results in constitutive activation of Hck. In contrast to phosphorylation of the COOH-termil regulatory tyrosine residue, autophosphorylation of a tyrosine residue (Tyr388) within the kise domain of Hck acts to positively regulate its catalytic activity. Thus, activation of Hck requires both disruption of the COOH-termil regulatory tyrosine-SH2 domain interaction and autophosphorylation of the regulatory tyrosine residue within the kise domain. The dysfunction or dysregulation of Hck may contribute to the pathogenesis of some human leukemias.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

HCK antibody

  Rabbit polyclonal HCK (Tyr521) antibody(Phospho-specific)  
HCK Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HCK antibody

  Rabbit polyclonal HCK Antibody (N-term)  
HCK Antibody (N-term) (Cat. #TA324975) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the HCK antibody detected the HCK protein (arrow). Rabbit IgG Purified Hu, Mky, Ms, Rt F, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

HCK antibody

  HCK Rabbit Polyclonal Antibody  
Western blot analysis of extracts of HL-60 cell lines, using HCK antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HCK antibody

  Rabbit Polyclonal Anti-HCK Antibody  
WB Suggested Anti-HCK Antibody; Titration: 1.0 ug/ml; Positive Control: Fetal Thymus Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

HCK antibody

  Rabbit Polyclonal Anti-HCK Antibody  
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: A549 cells, Primary antibody: (HCK Antibody) at dilution 1/450, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 15 seconds Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HCK antibody

Immunohistochemical analysis of paraffin-embedded human colon cancer (left) and ancreas cancer (right), showing cytoplasmic localization using HCK antibody Cat.-No  AM06200SU-N with DAB staining. Mouse IgG1 3D12E10 Ascites Hu P, WB
0.1 ml / €440.00
  Acris Antibodies GmbH

HCK (N-term) antibody

HCK Rabbit Ig Purified Hu WB
0.4 ml / €370.00
  Acris Antibodies GmbH

HCK pTyr521 antibody

Western blot analysis of extracts from JurKat cells using HCK (Phospho-Tyr521) Antibody AP55839PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms, Rt WB
0.1 ml / €340.00
  Acris Antibodies GmbH

HCK pTyr521 antibody

Western blot analysis of extracts from JurKat cells using HCK (Phospho-Tyr521) Antibody AP55839PU-N.The lane on the right is treated with the antigen-specific peptide. Rabbit Aff - Purified Hu, Ms, Rt WB
50 µl / €270.00
  Acris Antibodies GmbH

HCK pTyr410 antibody

Immunofluorescence analysis of HeLa cells, using HCK (Phospho-Tyr410) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF
50 µg / €230.00
  Assay Biotech

HCK pTyr410 antibody

Immunofluorescence analysis of HeLa cells, using HCK (Phospho-Tyr410) Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF
0.1 mg / €330.00
  Assay Biotech

HCK antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using HCK (Ab-410) Antibody Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF
50 µg / €230.00
  Assay Biotech

HCK antibody

Immunohistochemistry analysis of paraffin-embedded human brain tissue, using HCK (Ab-410) Antibody Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

HCK - human gene knockout kit via CRISPR

HCK - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

HCK - human gene knockout kit via CRISPR

HCK - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

HCK - human gene knockout kit via CRISPR

HCK - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

HCK - human gene knockout kit via CRISPR

HCK - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

HCK

  Recombinant protein of human hemopoietic cell kinase (HCK)  
HCK Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

HCK overexpression lysate

HCK overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

HCK overexpression lysate

HCK overexpression lysate  
0.1 mg / €280.00
  OriGene Technologies, Inc.

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