HCLS1 antibody

Principal name

HCLS1 antibody

Alternative names for HCLS1 antibody

LckBP1, Hematopoietic lineage cell-specific protein, Hematopoietic cell-specific LYN substrate 1

SwissProt ID

P14317 (Human), P49710 (Mouse)

Gene ID

3059 (HCLS1)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Can (Canine), Rt (Rat), Chk (Chicken), Ze (Zebrafish), African clawed frog, Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Goat, Mouse, Rabbit

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), Dot blot (Dot), Immunoprecipitation (IP)

Background of HCLS1 antibody

HS1 which is hematopoietic lineage cell-specific protein 1, is a substrate of protein tyrosine kinases in lymphocytes, it binds to F-actin, and promotes Arp2/3 complex-mediated actin polymerization. However, the mechanism for the interaction between HS1 and F-actin has not yet been fully characterized. HS1 contains 3.5 tandem repeats, a coiled-coil region, and an SH3 domain at the C terminus. Unlike cortactin, which is closely related to HS1 and requires absolutely the repeat domain for F-actin binding, an HS1 mutant with deletion of the repeat domain maintains a significant F-actin binding activity. Deletion of the coiled-coil region abolished the ability of HS1 to bind to actin filaments and to activate the Arp2/3 complex for actin nucleation and actin branching.

General readings

Ruzzene,M., et al., (2002) Biochem. J. 364 (Pt 1), 41-47

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

HCLS1 antibody

WB Suggested Anti-HCLS1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Jurkat cell lysateHCLS1 is supported by BioGPS gene expression data to be expressed in Jurkat Rabbit IgG Purified Hu P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

HCLS1 antibody

WB Suggested Anti-HCLS1 Antibody Titration: 1.25ug/ml; Positive Control: Jurkat cell lysateHCLS1 is supported by BioGPS gene expression data to be expressed in Jurkat Rabbit IgG Purified Hu P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HCLS1 antibody

Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 2A8 Hu E, P, WB
50 µg / €530.00
  Acris Antibodies GmbH

HCLS1 antibody

Immunoperoxidase of monoclonal antibody to HCLS1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml] Mouse IgG1 1A8 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

HCLS1 antibody

Immunoperoxidase of monoclonal antibody to HCLS1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml] Mouse IgG1 2A9 Purified Hu E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

HCLS1 antibody

Human Heart; Rabbit Anti-HCLS1 Antibody. Catalog Number: ARP31928. Paraffin Embedded Tissue: Human Heart. Cellular Data: Myocardial cells. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; HCLS1 antibody - N-terminal region (ARP31928_P050) in Human Heart cells using Immunohistochemistry Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt P, WB
50 µg / €350.00
  AVIVA Systems Biology

HCLS1 antibody

Human Heart; Rabbit Anti-HCLS1 Antibody. Catalog Number: ARP31928. Paraffin Embedded Tissue: Human Heart. Cellular Data: Myocardial cells. Antibody Concentration: 4.0-8.0 ug/ml. Magnification: 400X; HCLS1 antibody - N-terminal region (ARP31928_T100) in Human Heart cells using Immunohistochemistry Rabbit Aff - Purified Hu P, WB
0.1 mg / €310.00
  AVIVA Systems Biology

HCLS1 antibody

Western blot analysis of Raji cell lysates with anti-HCLS1 antibody (1:500). Rabbit Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HCLS1 antibody

Western blot analysis of extracts from JurKat cells, using HS1 (Ab-397)antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HCLS1 antibody

Western blot analysis of extracts of HEK-293 cell lines, using HCLS1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

HCLS1 antibody

WB Suggested Anti-HCLS1 Antibody Titration: 1.25ug/ml; Positive Control: Jurkat cell lysateHCLS1 is supported by BioGPS gene expression data to be expressed in Jurkat Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HCLS1 antibody

Western blot analysis of HS1 expression in Raji (1), Jurkat (2), WR19L (3) and PC12 (4) using AM26574AF-N. Mouse IgG1 3A3 Azide Free Hu, Ms, Rt IP, WB
0.1 mg / €380.00
  Acris Antibodies GmbH

HCLS1 pTyr397 antibody

Western blot analysis of extracts from JK cells (Lane 2), using HS1 (Phospho-Tyr397) Antibody AP55834PU-N.The lane on the left is treated with antigen-specific peptide. Rabbit Aff - Purified Hu WB
0.1 ml / €340.00
  Acris Antibodies GmbH

HCLS1 pTyr397 antibody

Western blot analysis of extracts from JK cells (Lane 2), using HS1 (Phospho-Tyr397) Antibody AP55834PU-N.The lane on the left is treated with antigen-specific peptide. Rabbit Aff - Purified Hu WB
50 µl / €270.00
  Acris Antibodies GmbH

HCLS1 antibody

HCLS1 MaxPab polyclonal antibody. Western Blot analysis of HCLS1 expression in human liver. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

HCLS1 antibody

Immunoprecipitation of HCLS1 transfected lysate using anti-HCLS1 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with HCLS1 MaxPab mouse polyclonal antibody (B01) (H00003059-B01). Rabbit Serum Hu E(detection), IP
0.1 ml / €380.00
  Abnova Taiwan Corp.

HCLS1 antibody

HCLS1 monoclonal antibody (M01), clone 3D5 Western Blot analysis of HCLS1 expression in K-562 ( Cat # L009V1 ). Mouse IgG1 3D5 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

HCLS1 antibody

Immunoperoxidase of monoclonal antibody to HCLS1 on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 3 ug/ml] Mouse IgG1 2A6 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

HCLS1 antibody

Immunoperoxidase of monoclonal antibody to HCLS1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml] Mouse IgG1 3F4 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

HCLS1 antibody

Immunoperoxidase of monoclonal antibody to HCLS1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml] Mouse IgG1 2A8 Purified Hu E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

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