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HCN3 antibody

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Principal name

HCN3 antibody

Alternative names for HCN3 antibody

KIAA1535, Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3

SwissProt ID

O88705 (Mouse), Q9JKA8 (Rat), Q9P1Z3 (Human)

Gene ID

57657 (HCN3), 15168 (Hcn3), 114245

Ncbi ID

NP_065948, NP_065948.1, AAI56231.1, NP_032253.1, NP_032253, NP_446137.1, NM_008227, NM_053685, NM_020897

Available reactivities

Bov (Bovine), Hu (Human), Ms (Mouse), Rt (Rat), Can (Canine), Por (Porcine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rabbit, Goat, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of HCN3 antibody

Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces.
There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligand- gated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels; each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward-rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2).
Specifically, hyperpolarization-activated cation channels of the HCN gene family contribute to spontaneous rhythmic activity in both the heart and brain (3).

ARP35310_P050 - HCN3
Human COLO205; WB Suggested Anti-HCN3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: COLO205 cell lysate; HCN3 antibody - middle region (ARP35310_P050) in Human COLO205 cells using Western Blot
AP23226PU-N - HCN3
AP23226PU-N HCN3 antibody staining of Rat Kidney lysate at 1 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
75-175 - HCN3
Adult rat brain membrane immunoblot
75-175 - HCN3
Adult rat hippocampus immunohistochemistry
73-175 - HCN3
Adult rat brain membrane immunoblot
73-175 - HCN3
Adult rat hippocampus immunohistochemistry
TA311496 - HCN3
(0.01ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
TA326475 - HCN3
Western blot analysis of HCN3 in rat brain membrane lysates using a 1:1000 dilution of the antibody
TA326475 - HCN3
IF analysis of HCN3 in human hippocampal tissues using the antibody
TA328956 - HCN3
Western blot analysis of HCN3 in rat brain lysate: 1. Anti-HCN3 antibody , (1:200). 2. Anti-HCN3 antibody, preincubated with the control peptide antigen.
TA328956 - HCN3
Expression of HCN3 in Rat Dorsal Root Ganglia (DRG) Immunohistochemical staining of frozen section of rat DRG using Anti-HCN3 antibody , (1:50) followed by mouse anti-rabbit Alexa Fluor 555 (red). Staining is present in neuronal cell bodies. Hoechst 33342 (blue) is used as counterstain.
TA338594 - HCN3
WB Suggested Anti-HCN3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: COLO205 cell lysate

Items 1 to 20 of 22 total

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

HCN3 antibody
AP23226PU-N HCN3 antibody staining of Rat Kidney lysate at 1 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, WB
0.1 mg / €350.00
  Acris Antibodies GmbH
 

Acris recommends this product for Western blotting.

HCN3 antibody
  Goat Anti-HCN3 (aa 715-728) Antibody  
(0.01ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Purified Bov, Can, Ms, Por, Rt WB
0.1 mg / €360.00
  OriGene Technologies, Inc.

HCN3 antibody
  Mouse Monoclonal Anti-HCN3 Antibody  
Western blot analysis of HCN3 in rat brain membrane lysates using a 1:1000 dilution of the antibody Mouse IgG1 S141-28 Purified Ms, Rt C, ICC/IF, WB
0.1 mg / €410.00
  OriGene Technologies, Inc.
+1 additional image

HCN3 antibody
  Rabbit polyclonal Anti-HCN3  
Western blot analysis of HCN3 in rat brain lysate: 1. Anti-HCN3 antibody , (1:200). 2. Anti-HCN3 antibody, preincubated with the control peptide antigen. Rabbit Purified Rt WB
0.2 ml / €622.00
  OriGene Technologies, Inc.
+1 additional image

HCN3 antibody
  Rabbit Polyclonal Anti-HCN3 Antibody  
WB Suggested Anti-HCN3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: COLO205 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €360.00
  OriGene Technologies, Inc.

HCN3 antibody
Adult rat brain membrane immunoblot Mouse IgG1 N141/28 Supernatant Ms, Rt C, ICC/IF, WB
5 ml / €460.00
  Antibodies Incorporated
+1 additional image

NeuroMab

HCN3 antibody
Adult rat brain membrane immunoblot Mouse IgG1 N141/28 Purified Ms, Rt C, ICC/IF, WB
0.1 ml / €540.00
  Antibodies Incorporated
+1 additional image

NeuroMab

HCN3 antibody
HCN3 Rabbit Serum Hu C, E, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

CRISPR

Catalog No. Species Donor Vector  

HCN3 - human gene knockout kit via CRISPR
HCN3 - human gene knockout kit via CRISPR Human GFP-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

HCN3 - human gene knockout kit via CRISPR
HCN3 - human gene knockout kit via CRISPR Human mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

HCN3 - human gene knockout kit via CRISPR
HCN3 - human gene knockout kit via CRISPR Human Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

HCN3 - human gene knockout kit via CRISPR
HCN3 - human gene knockout kit via CRISPR Human RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Hcn3 - mouse gene knockout kit via CRISPR
Hcn3 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Hcn3 - mouse gene knockout kit via CRISPR
Hcn3 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Hcn3 - mouse gene knockout kit via CRISPR
Hcn3 - mouse gene knockout kit via CRISPR Mouse Luciferase-Puro
1 kit / €1,290.00
  OriGene Technologies, Inc.

Hcn3 - mouse gene knockout kit via CRISPR
Hcn3 - mouse gene knockout kit via CRISPR Mouse RFP-BSD
1 kit / €1,290.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, HCN3 (mGFP-tagged)-Human hyperpolarization activated cyclic nucleotide-gated potassium channel 3 (HCN3), 200 uL, >10^7 TU/mL
Lenti ORF particles, HCN3 (mGFP-tagged)-Human hyperpolarization activated cyclic nucleotide-gated potassium channel 3 (HCN3), 200 uL, >10^7 TU/mL  
200 µl / €1,741.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, HCN3 (Myc-DDK-tagged)-Human hyperpolarization activated cyclic nucleotide-gated potassium channel 3 (HCN3), 200 uL, >10^7 TU/mL
Lenti ORF particles, HCN3 (Myc-DDK-tagged)-Human hyperpolarization activated cyclic nucleotide-gated potassium channel 3 (HCN3), 200 uL, >10^7 TU/mL  
200 µl / €1,566.00
  OriGene Technologies, Inc.
 

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Hcn3 (GFP-tagged) - Mouse hyperpolarization-activated, cyclic nucleotide-gated K+ 3 (Hcn3), 200 uL, >10^7 TU/mL
Lenti ORF particles, Hcn3 (GFP-tagged) - Mouse hyperpolarization-activated, cyclic nucleotide-gated K+ 3 (Hcn3), 200 uL, >10^7 TU/mL  
200 µl / €1,535.00
  OriGene Technologies, Inc.
 

Lenti ORF particles, Hcn3 (Myc-DDK-tagged) - Mouse hyperpolarization-activated, cyclic nucleotide-gated K+ 3 (Hcn3), 200 uL, >10^7 TU/mL
Lenti ORF particles, Hcn3 (Myc-DDK-tagged) - Mouse hyperpolarization-activated, cyclic nucleotide-gated K+ 3 (Hcn3), 200 uL, >10^7 TU/mL  
200 µl / €1,381.00
  OriGene Technologies, Inc.
 

Items 1 to 20 of 22 total

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22 products - FILTER YOUR RESULTS!

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Product Type
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Clonality
  1. Polyclonal (5)
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Target
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Reactivity
  1. Rat (Rt) (7)
  2. Mouse (Ms) (6)
  3. Bovine (Bov) (3)
  4. Canine (Can) (3)
  5. Human (Hu) (3)
  6. Porcine (Por) (2)
  7. Equine (Eq) (1)
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Applications
  1. Western blot / Immunoblot (WB) (8)
  2. Frozen sections (C) (4)
  3. Immunocytochemistry/​Immunofluorescence (ICC/IF) (3)
  4. Enzyme Immunoassay (E) (2)
Presentation
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  1. Mouse (3)
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Images
  1. 1 image (7)
Images: Applications
  1. Western blot / Immunoblot (WB) (7)
  2. Frozen sections (C) (2)
  3. Immunofluorescence (IF) (1)
  4. IHC (1)
Species (CRISPR)
  1. Human (4)
  2. Mouse (4)
Donor Vector
  1. GFP-Puro (2)
  2. Luciferase-Puro (2)
  3. RFP-BSD (2)
  4. mBFP-Neo (2)
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  1. Acris - OriGene (20)
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Service
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