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HCN3 antibody

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Principal name

HCN3 antibody

Alternative names for HCN3 antibody

KIAA1535, Potassium/sodium hyperpolarization-activated cyclic nucleotide-gated channel 3

SwissProt ID

O88705 (Mouse), Q9JKA8 (Rat), Q9P1Z3 (Human)

Gene ID

57657 (HCN3), 15168 (Hcn3), 114245

Ncbi ID

9606, NP_065948, NP_032253, NP_065948.1, AAI56231.1, NP_032253.1, NP_446137.1

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), Por (Porcine), Eq (Equine), GP (Guinea Pig), Rb (Rabbit)

Available hosts

Rat, Rabbit, Mouse, Goat

Available applications

Paraffin Sections (P), Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Frozen Sections (C), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of HCN3 antibody

Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces.
There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligand- gated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels; each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward-rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2).
Specifically, hyperpolarization-activated cation channels of the HCN gene family contribute to spontaneous rhythmic activity in both the heart and brain (3).

13745-1-AP - HCN3
Human kidney tissue were subjected to SDS PAGE followed by western blot with 13745-1-AP(HCN3 antibody) at dilution of 1:800
ARP35310_P050 - HCN3
Human COLO205; WB Suggested Anti-HCN3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: COLO205 cell lysate; HCN3 antibody - middle region (ARP35310_P050) in Human COLO205 cells using Western Blot
AP23226PU-N - HCN3
AP23226PU-N HCN3 antibody staining of Rat Kidney lysate at 1 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
NBP1-81402 - HCN3
Immunohistochemistry-Paraffin: HCN3 Antibody [NBP1-81402] Immunohistochemical staining of human duodenum shows strong cytoplasmic and membranous positivity in glandular cells.
GTX41987 - HCN3
IF analysis of HCN3 in human hippocampal tissues using GTX41987.
GTX41987 - HCN3
Western blot analysis of HCN3 in rat brain membrane lysates using a 1:1000 dilution of GTX41987.
GTX47663 - HCN3
Detection of HCN3 in COLO205 using anti-HCN3 antibody (GTX47663) at 1 µg/mL.
GTX79455 - HCN3
IHC-P analysis of human brain tissue with (left) or without (right) HCN3 antibody [TLL6C5] at a dilution of 1:20. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes.
GTX79455 - HCN3
IHC-P analysis of human colon carcinoma tissues with (left) or without (right) HCN3 antibody [TLL6C5] at a dilution of 1:20. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes.
75-175 - HCN3
Adult rat brain membrane immunoblot
75-175 - HCN3
Adult rat hippocampus immunohistochemistry
73-175 - HCN3
Adult rat brain membrane immunoblot
73-175 - HCN3
Adult rat hippocampus immunohistochemistry

13 Item(s)

per page

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

HCN3 antibody
AP23226PU-N HCN3 antibody staining of Rat Kidney lysate at 1 µg/ml (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Rt E, WB
0.1 mg / €340.00
  Acris Antibodies GmbH
 

Acris recommends this product for Western blotting.

HCN3 antibody
(0.01ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Purified Bov, Can, Ms, Por, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HCN3 antibody
Western blot analysis of HCN3 in rat brain membrane lysates using a 1:1000 dilution of the antibody Mouse IgG1 S141-28 Purified Ms, Rt C, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.
+1 additional image

HCN3 antibody
Western blot analysis of HCN3 in rat brain lysate: 1. Anti-HCN3 antibody , (1:200). 2. Anti-HCN3 antibody, preincubated with the control peptide antigen. Rabbit Purified Rt WB
0.2 ml / €435.00
  OriGene Technologies, Inc.
+1 additional image

HCN3 antibody
WB Suggested Anti-HCN3 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: COLO205 cell lysate Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

HCN3 antibody
Adult rat brain membrane immunoblot Mouse IgG1 N141/28 Supernatant Ms, Rt C, ICC/IF, WB
5 ml / €320.00
  Antibodies Incorporated
+1 additional image

NeuroMab

HCN3 antibody
Adult rat brain membrane immunoblot Mouse IgG1 N141/28 Purified Ms, Rt C, ICC/IF, WB
0.1 ml / €380.00
  Antibodies Incorporated
+1 additional image

NeuroMab

HCN3 antibody
HCN3 Rabbit Serum Hu C, E, WB
0.2 ml / €400.00
  Acris Antibodies GmbH

HCN3 antibody
HCN3 Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

HCN3 antibody
Human COLO205; WB Suggested Anti-HCN3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: COLO205 cell lysate; HCN3 antibody - middle region (ARP35310_P050) in Human COLO205 cells using Western Blot Rabbit Aff - Purified Bov, Hu, Ms, Rt WB
50 µg / €350.00
  AVIVA Systems Biology

HCN3 antibody
HCN3 Rat IgG1 TLL6C5 Ascites Hu, Ms, Rt P
0.1 ml / €400.00
  Novus Biologicals Inc.

HCN3 antibody
HCN3 Goat Aff - Purified Rt E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

HCN3 antibody
Immunohistochemistry-Paraffin: HCN3 Antibody [NBP1-81402] Immunohistochemical staining of human duodenum shows strong cytoplasmic and membranous positivity in glandular cells. Rabbit IgG Aff - Purified Hu P
0.1 ml / €500.00
  Novus Biologicals Inc.

13 Item(s)

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13 products - FILTER YOUR RESULTS!

Available Filters

Product Type
  1. Primary Antibody (13)
Clonality
  1. Polyclonal (9)
  2. Monoclonal (4)
Target
  1. HCN3 (13)
Reactivity
  1. Rat (Rt) (10)
  2. Mouse (Ms) (8)
  3. Human (Hu) (7)
  4. Bovine (Bov) (4)
  5. Canine (Can) (3)
  6. Porcine (Por) (2)
  7. Equine (Eq) (1)
  8. Guinea Pig (GP) (1)
  9. Rabbit (Rb) (1)
Applications
  1. Western blot / Immunoblot (WB) (11)
  2. Frozen sections (C) (4)
  3. Enzyme Immunoassay (E) (3)
  4. Immunocytochemistry/​Immunofluorescence (ICC/IF) (3)
  5. Paraffin Sections (P) (2)
Presentation
  1. Purified (5)
  2. Aff - Purified (4)
  3. Ascites (1)
  4. Serum (1)
  5. Supernatant (1)
Host
  1. Rabbit (5)
  2. Mouse (4)
  3. Goat (3)
  4. Rat (1)
Images
  1. 1 image (9)
Images: Applications
  1. Western blot / Immunoblot (WB) (8)
  2. Frozen sections (C) (2)
  3. Immunofluorescence (IF) (1)
  4. IHC (1)
  5. Paraffin Sections (P) (1)
Manufacturer
  1. Acris - OriGene (6)
  2. Novus Biologicals Inc. (3)
  3. Antibodies Incorporated (2)
  4. AVIVA Systems Biology (1)
  5. Abnova Taiwan Corp. (1)
Service
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