HDAC2 antibody

Principal name

HDAC2 antibody

Alternative names for HDAC2 antibody

Histone deacetylase 2, HD2

SwissProt ID

P56519 (Chick), P70288 (Mouse), Q92769 (Human)

Gene ID

3066 (HDAC2), 395635

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Chk (Chicken), African clawed frog, Can (Canine), Mky (Monkey), Hst (Hamster), Eq (Equine), GP (Guinea Pig), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Mouse

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), ChIP assay (ChIP), Immunoprecipitation (IP), Frozen Sections (C), ELISA (detection) (E(detection)), Flow Cytometry (F)

Background of HDAC2 antibody

The basic repeating unit of chromatin is the nucleosome, which is composed of a protein octamer containing two each of the core histones H2A, H2B, H3, and H4, surrounded by approximately 146 base pairs of DNA. Reversible acetylation of highly conserved lysine residues in the N-terminal tail domains of core histones plays an important role in transcriptional regulation, cell cycle progression, and development events. Several histone acetyltransferases (HATs) catalyze this acetylation reaction (e.g. GCN5, PCAF, p300/CBP, TAFII250, P/CAF, SRC-1, BRCA-2). Acetylation of the core histones is generally considered to be associated with gene activation, probably through maintenance of the unfolded structure of transcribing nucleosomes. Histone acetylation is a dynamic process in which levels are determined by the net activities of HATs and the competing enzymes histone deacetylases (HDACs). Both activities are associated with the nuclear matrix. Eleven different mammalian HDACs have been described. HDACs 1-3 & 8 (Class I) are similar to yeast Rpd3 protein, while HDACs 4-7, 9 & 10 (Class II) are similar to yeast Hda1 protein. The activities of the histone deacetylases are often, but not always, associated with transcriptional repression and nucleosome condensation. HDAC1, HDAC2 and several others are the catalytic subunits of different multiprotein regulatory complexes. Other components of such complexes may include: corepressors such as mSin3, N-CoR, SMRT, associated proteins such as SAP18, SAP30, RbAp46, RbAp48, and c-Ski oncogenic protein (involved in DNA methylation). Nucleosome remodeling and deacetylation (NRD) complexes containing HDAC1, HDAC2, Mi-2 (CH3, CH4) dermatomyositis specific autoantigen, and MAT2 (metastasis-associated protein) (related to MAT1) have been described. It is therefore assumed that ATP-dependent nucleosome remodeling activity and histone deacetylation may be interconnected or interdependent. Recruitment of the multiprotein complexes to promoter sites occurs by many sequence specific DNA-binding proteins such as unliganded nuclear hormone receptors, DP1-E2F, YY1, and Rb family of transcription factors, transcriptional repressors, and tumor suppressors (e.g. BRCA1). Aberrant recruitment of HDACs by various oncoproteins may occur in certain neoplastic diseases. It has been found that inhibition ofHDAC2 activity by valporic acid induces proteosomal degradation of HDAC2.

General readings

Ishikawa, F., et al. Oncogene 29(6):909-919(2010)
Bush, E.W., et al. Circ. Res. 106(2):272-284(2010)
Krishnan, M., et al. Oncogene 29(2):305-312(2010)
Lehmann, A., et al. BMC Cancer 9, 395 (2009)
Hassig, C.A., et al. Proc. Natl. Acad. Sci. U.S.A. 95(7):3519-3524(1998)

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

HDAC2 antibody

Immunohistochemical staining of paraffin-embedded Human endometrium tissue using anti-HDAC2 rabbit polyclonal antibody. (TA590734) Rabbit IgG Purified Can, Hu, Mky, Ms, Rt E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HDAC2 antibody

Immunohistochemical staining of paraffin-embedded Human colon tissue within the normal limits using anti-HDAC2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120°C for 3 min, TA808327)(1:150) Mouse IgG1 OTI7E10 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HDAC2 pSer394 antibody

Figure 1. Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using HDAC2 (pSer394) antibody AP08014PU. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

HDAC2 pSer394 antibody

Figure 1. Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using HDAC2 (pSer394) antibody AP08014PU. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
50 µl / €240.00
  Acris Antibodies GmbH

HDAC2 (C-term) antibody

Human tonsil stained with Anti-HDAC2 antibody (Cat# AP15593PU). Rabbit Purified Hu, Ms, Rt P, WB
0.5 ml / €410.00
  Acris Antibodies GmbH

HDAC2 (C-term) antibody

Human tonsil stained with Anti-HDAC2 antibody (Cat# AP15593PU). Rabbit Purified Hu, Ms, Rt P, WB
1 ml / €580.00
  Acris Antibodies GmbH

HDAC2 (C-term) antibody

Human tonsil stained with Anti-HDAC2 antibody (Cat# AP15593PU). Rabbit Purified Hu, Ms, Rt P, WB
0.1 ml / €190.00
  Acris Antibodies GmbH

HDAC2 antibody

Western blot analysis of extracts from HepG2 cells, using HDAC2 (Ab-394) Antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HDAC2 antibody

Predicted band size: 55 kDa . Positive control: 293 and Hela cells lysate. Recommended dilution: 1/ 500-1000. (Gel: 10%SDS-PAGE Lane1: 293 cells lysate Lane2: Hela cells lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HDAC2 antibody

Predicted cell location: Nucleus. Positive control: Human breast and colon cancer tissue. Recommended dilution: 1/15-50  The image on the left is immunohistochemistry of paraffin-embedded Human breast and colon cancer tissue using HDAC2 antibody at dilution 1/17, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

HDAC2 antibody

Predicted cell location: Nucleus. Positive control: Human ovarian and colon cancer tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded Human ovarian and colon cancer tissue using HDAC2 antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification:x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

HDAC2 (Center) antibody

HDAC2 Antibody (Center) (Cat. #TA325112) western blot analysis in SH-SY5Y cell line lysates (35ug/lane).This demonstrates the HDAC2 antibody detected the HDAC2 protein (arrow). Rabbit Ig Aff - Purified Hu F, ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

HDAC2 antibody

Western blot analysis of HDAC2 expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HDAC2 antibody

Western blot analysis of extracts of various cell lines, using HDAC2 antibody. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HDAC2 antibody

HDAC2 Antibody (Center) (Cat.# TA328156) western blot analysis in Hela cell lysate (35ug/lane). This demonstrates that the HDAC2 antibody detected HDAC2 protein (arrow). Mouse IgG1 1194CT18.5.1 Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

HDAC2 antibody

WB Suggested Anti-HDAC2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: HT1080 cell lysate Rabbit IgG Purified Hu ChIP, P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

HDAC2 antibody

Host:Rabbit; Target Name:HDAC2; Sample Tissue:Human Adult Placenta; Antibody Dilution: 1.0ug/ml Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

HDAC2 antibody

WB using the antibody against HDAC2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 55 kDa); the marker (in kDa) is shown on the left. Mouse IgG1 Monoclol Purified Hu
50 µg / €325.00
  OriGene Technologies, Inc.

HDAC2 antibody

Western blot analysis of HDAC2 in HeLa cell lysate with HDAC2 antibody at (A) 0.5 and (B) 1 ug/mL. Rabbit IgG Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HDAC2 Control Peptide

HDAC2 Purified
50 µg / €190.00
  Acris Antibodies GmbH

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