HDAC3 antibody

Principal name

HDAC3 antibody

Alternative names for HDAC3 antibody

Histone deacetylase 3, RPD3-2, SMAP45, HD3

SwissProt ID

O15379 (Human), O88895 (Mouse), P56520 (Chick), Q6IRL9 (Xenla), Q6P6W3 (Rat)

Gene ID

8841 (HDAC3), 84578 (Hdac3)

Available reactivities

Can (Canine), Hst (Hamster), Hu (Human), Rt (Rat), Ms (Mouse), Chk (Chicken), Bov (Bovine), Por (Porcine)

Available hosts

Rabbit, Mouse, Goat

Available applications

Western blot / Immunoblot (WB), Paraffin Sections (P), Immunoprecipitation (IP), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), ELISA (detection) (E(detection)), ChIP assay (ChIP), Frozen Sections (C), Dot blot (Dot)

Background of HDAC3 antibody

Acetylation of the histone tail causes chromatin to adopt an "open" conformation, allowing increased accessibility of transcription factors to D. The identification of histone acetyltransferases (HATs) and their large multiprotein complexes has yielded important insights into how these enzymes regulate transcription (1,2). HAT complexes interact with sequence-specific activator proteins to target specific genes. In addition to histones, HATs can acetylate nonhistone proteins, suggesting multiple roles for these enzymes (3). In contrast, histone deacetylation promotes a "closed" chromatin conformation and typically leads to repression of gene activity (4). Mammalian histone deacetylases can be divided into three classes on the basis of their similarity to various yeast deacetylases (5). Class I proteins (HDACs 1, 2, 3, and 8) are related to the yeast Rpd3-like proteins, those in class II (HDACs 4, 5, 6, 7, 9, and 10) are related to yeast Hda1-like proteins, and class III proteins are related to the yeast protein Sir2. Inhibitors of HDAC activity are now being explored as potential therapeutic cancer agents (6,7).HDAC3 is a nuclear and cytoplasmic protein that deacetylates both histone (H2A, H3, H4) and non-histone substrates (RelA, SRY, p53, MEF2, PCAF and p300/CBP) (8). HDAC3 deacetylase activity is stimulated by interactions with the N-CoR and SMRT co-repressor proteins. Together, these three proteins form a functiol complex that represses transcription associated with nuclear hormone receptors and other transcription factors, including Rev-Erb, COUP-TF, DAX1, MAD and Pit-1 (8,9). Phosphorylation of HDAC3 on Ser424 by casein kise 2 (CK2) also increases HDAC3 deacetylase activity (9). Subsequently, de-phosphorylation by protein phosphatase 4 (PP4) decreases HDAC3 activity (9).1. Marmorstein, R. (2001) Cell Mol Life Sci 58, 693-703.2. Gregory, P.D. et al. (2001) Exp Cell Res 265, 195-202.3. Liu, Y. et al. (2000) Mol Cell Biol 20, 5540-53.4

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

HDAC3 antibody

Western blot analysis of extracts from HepG2 cells, using HDAC3 antibody .The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HDAC3 antibody

HDAC3 monoclonal antibody (M02), clone 3E11. Western Blot analysis of HDAC3 expression in NIH/3T3 ( Cat # L018V1 ). Mouse IgG2a 3E11 Purified Hu, Ms, Rt E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

HDAC3 antibody

Immunofluorescence of monoclonal antibody to HDAC3 on HeLa cell. [antibody concentration 10 ug/ml] Mouse IgG2a 2A3 Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

HDAC3 antibody

Predicted band size: 49 kDa . Positive control: 293T cell lysate. Recommended dilution: 1/500-2000. (Gel: 8%SDS-PAGE Lysate: 40 ug Primary antibody: 1/200 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HDAC3 antibody

Western blot analysis of A549, NIH/3T3, Raji, 293T and Hela cell using TA321609 (HDAC3 Antibody) at dilution 1/200 Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HDAC3 antibody

Western blot analysis of HDAC3 expression in NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HDAC3 antibody

Western blot analysis of extracts of various cell lines, using HDAC3 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

HDAC3 antibody

Western Blot: HDAC3 Antibody [TA336265] - Analysis of HDAC3 in HeLa cell lysate with anti-HDAC3 this antibody. Rabbit Purified Hu C, ChIP, ICC/IF, IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HDAC3 antibody

Western blot detection of HDAC3 in THP-1,LNCAP,MCF7,PC12,293,Jurkat,3T3 and Hela cell lysates using HDAC3 mouse mAb (1:1000 diluted).Predicted band size:49KDa.Observed band size:49KDa. Mouse IgG1 3G3-H6-H10 Purified WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HDAC3 antibody

Western blot analysis on NIH-3T3 cell lysate using HDAC3 Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HDAC3 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC3 (RC200605, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC3. Mouse IgG2a OTI4E10 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HDAC3 antibody

Confocal Immunofluorescence analysis of Hela cells using HDAC3 antibody Cat.-No AM06267SU-N (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Mouse IgG2a 7G6C5 Ascites Hu ICC/IF, WB
0.1 ml / €440.00
  Acris Antibodies GmbH

HDAC3 antibody

Immunohistochemical analysis of paraffin-embedded human esophagus cancer (left) and breast carcinoma tissue (right), showing nuclear localization with DAB staining using HDAC3 antibody Cat.-No AM06268SU-N Mouse IgG1 3A7B5 Ascites Hu E, P, WB
0.1 ml / €440.00
  Acris Antibodies GmbH

HDAC3 (224-428) antibody

Brain, cerebellum, Human: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG1 3A7B5 Ascites Hu E, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

HDAC3 antibody

IHC Image of AM20548SU-N
Brain, cerebellum, Human: Formalin-Fixed, Paraffin-Embedded (FFPE) Mouse IgG2a 7G6C5 Ascites Hu E, ICC/IF, P, WB
50 µl / €470.00
  Acris Antibodies GmbH

HDAC3 control peptide

HDAC3 Purified
50 µg / €190.00
  Acris Antibodies GmbH

HDAC3 antibody

Western blot analysis of HDAC-3 expression in Jurkat cell lysate. Rabbit Aff - Purified Hu, Ms, Rt P, WB
0.1 mg / €370.00
  Acris Antibodies GmbH

HDAC3 (2-17) antibody

Placenta: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu ChIP, IP, P, WB
50 µg / €470.00
  Acris Antibodies GmbH

HDAC3 antibody

Immunohistochemistry analyzes of SMAP45 antibody (AP20235PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms, Rt ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

HDAC3 antibody

AP33509PU-N (0.1µg/ml) staining of Jurkat nuclear lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat Aff - Purified Bov, Can, Hu, Ms, Por, Rt WB
0.1 mg / €340.00
  Acris Antibodies GmbH

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