HDAC4 antibody
Principal name
HDAC4 antibody
Alternative names for HDAC4 antibody
Histone deacetylase 4, HD4, KIAA0288
Ncbi ID
NP_006028.2, 9606, NP_006028, AAH39904.1, NM_006037, NP_997108.1, NM_207225
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Paraffin Sections (P), ChIP assay (ChIP), Immunoprecipitation (IP), ELISA (detection) (E(detection))
Background of HDAC4 antibody
Eukaryotic chromatin is composed of highly packed chromosomal DNA that is stabilized by many nuclear proteins. The chromatin DNA is constantly packed and unpacked during gene expression and is controlled a host of proteins. The acetylation of lysine residues in histones, and/or of transcription factors, is an important posttranslational mechanism of transcriptional regulation. The acetylated histone protein produced confirmation changes that confer accessibility of the homology to yeast sequences DNA template to the transcriptional machinery for protein expression. During damage to double stranded DNA, a number of proteins are recruited to nuclear foci including HDAC4, 53BP1 and Rad51 proteins. The HDAC4 gradually disappear from foci in repair proficient cells but linger on for some time in repair deficient cells suggesting that hDCA4 is linked to DNA repair (1). Silencing CA4 by SiRNA also resulted in decrease level of partner protein 53BP1 and abrogated DNA damage-induced G2 delay in radio sensitized HeLa cells (1). Nucleocytoplasmic trafficking is an important aspect of HDAC4 functioning, and binding of 14-3-3 proteins is necessary for its cytoplasmic retention. The N-terminal 118 amino acids modulate the nuclear localization but residues 244-279 constitute a strong nuclear localization signal. A hydrophobic motif located at C-terminus serves as nuclear export signal that is necessary for cytoplasmic retentionof HDAC4 (2).
Histone deacetylases (HDACs) catalyze the deacetylation of histone and non-histone substrates, and in general act to repress transcription as part of larger corepressor complexes. Eighteen mammalian HDACs have been identified to date, and they are grouped into four classes based on their respective homology to yeast deacetylases (3). The class II HDACs can be further subdivided into class IIa (HDAC4, -5, -7, and -9) and class IIb (HDAC6 and -10), based on the presence in class IIa members of conserved motifs in the N-terminal domain, and extended C terminal tails, that are essential in regulating their function (4). The HDCA4 is a transcriptional corepressor that exist in distinct corepressor complex than class I proteins and it shuttles between nucleus and cytoplasmic compartment involving active nuclear transport. In the nucleus, HDAC4 associates with the myocyte enhancer factor MEF2A. Binding of HDAC4 to MEF2A results in the repression of MEF2A transcriptional activation. HDAC4 has also been shown to interact with other deacetylases such as HDAC3 as well as the corepressors NcoR and SMART. HDCA4 has a direct role in proproliferation of colon cancer by Sp1-dependent recruitment of HDCA4 to the p21 promotor and acting in concert with HDAC4-HDCA3-N-CoR/SMART co repressor complex resulting in transcriptional repression (5).
Western blot analysis of HDAC-4 in mouse brain tissue lysate. An 140 kDa band can be detected.
Western blot analysis of HDAC-4 expression in Jurkat cell lysate.
Figure 1. Western blot analysis of extracts from Jurkat cells using HDAC4 antibody (Lane 1 and 2) and HDAC4 (phospho-Ser632) antibody AP02464PU (Lane 3 and 4).
Immunofluorescence staining of methanol-fixed HeLa cells using HDAC4 antibody AP02668PU (Red).
Western Blot analysis of extracts from HepG2 cells using HDAC4 antibody
Western blot (WB) analysis of HDAC4 antibody (Cat.-No.: AP06392PU-N) in extracts from Jurkat cells.
Immunohistochemistry Image: Breast
Western blot analysis of HDAC-4 in Jurkat cell lysate with HDAC4 polyclonal antibody ( Cat # PAB0181 ) .
The HDAC4 polyclonal antibody ( Cat # PAB2329 ) is used in Western blot to detect HDAC4 in mouse brain tissue lysate.
Immunoperoxidase of monoclonal antibody to HDAC4 on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3 ug/ml]
HDAC4 monoclonal antibody (M03), clone 2B11. Western Blot analysis of HDAC4 expression in Jurkat ( Cat # L017V1 ).
Detection limit for recombinant GST tagged HDAC4 is approximately 0.1ng/ml as a capture antibody.
Western Blot analysis of HDAC4 expression in transfected 293T cell line (H00009759-T01) by HDAC4 MaxPab polyclonal antibody.Lane 1: HDAC4 transfected lysate(106.92 KDa).Lane 2: Non-transfected lysate.
Western blot (WB) analysis of p-HDAC4 (pSer632) antibody (Cat.-No.: AP01745PU-N) in extracts from Jurkat cells.
Western blot analysis of anti-HDAC4 Pab (Cat. #AP11127PU-N) to detect HDAC4 in mouse brain tissue lysate.
Skin: Formalin-Fixed Paraffin-Embedded (FFPE)
Lung, respiratory epithelium: Formalin-Fixed Paraffin-Embedded (FFPE)
Breast: Formalin-Fixed Paraffin-Embedded (FFPE)
Western blot analysis of HDAC4 in mouse braintissue lysate. An 140 kDa band can be detected.
Western Blot analysis of HDAC4 expression in transfected 293T cell line (H00009759-T02) by HDAC4 MaxPab polyclonal antibody.Lane 1: HDAC4 transfected lysate(106.40 KDa).Lane 2: Non-transfected lysate.
Western blot of AP10099PU-N (1:500).
Human HeLa; WB Suggested Anti-HDAC4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Hela cell lysate; HDAC4 antibody - N-terminal region (ARP34229_P050) in Human HeLa cells using Western Blot
Human HeLa; WB Suggested Anti-HDAC4 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:12500. Positive Control: Hela cell lysate; HDAC4 antibody - middle region (ARP34230_P050) in Human HeLa cells using Western Blot
Western blot analysis using HDAC4 mouse mAb against Hela (1), Jurkat (2) cell lysate.
ELISA: HDAC4 Antibody
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC4 (RC211495, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC4.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC4 (RC211495, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC4.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC4 (RC211495, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC4.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC4 (RC211495, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC4.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC4 (RC211495, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC4.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC4 (RC211495, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC4.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC4 (RC211495, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC4.
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC4 (RC211495, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC4.
Western blot analysis of extracts from HeLa/Jurkat cells, using HDAC4 (TA312828) Antibody. The lane on the right is treated with the synthesized peptide
Western blot analysis of extracts from Hela cells (Lane 2), using HDAC4 (TA312828) Antibody. The lane on the left is treated with synthesized peptide.
Predicted cell location: Cytoplasm. Positive control: Human tonsil tissue. Recommended dilution: 1/25-100 The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using HDAC4 antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification: x200)
Predicted cell location: Cytoplasm. Positive control: Human tonsil tissue. Recommended dilution: 1/10-50 The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using HDAC4 antibody at dilution 1/15, on the right is treated with the synthetic peptide. (Original magnification: x200)
HDAC4 Antibody (C-term) (Cat. #TA324523) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the HDAC4 antibody detected the HDAC4 protein (arrow).
HDAC4 Antibody (C-term) (Cat. #TA324523)immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of HDAC4 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
IF image of Hela cell stained with HDAC4 Antibody (C-term)(Cat#TA324523).Hela cells were incubated with HDAC4 primary antibody (1:25, 1 h at 37?). For secondary antibody, Alexa Fluor® 488 conjugated donkey anti-rabbit antibody (green) was used (1:400).Cytoplasmic actin was counterstained with Alexa Fluor® 555 (red) conjugated Phalloidin (7 units/ml). Nuclei were counterstained with DAPI (blue) . HDAC4 immunoreactivity is localized to Nucleus significantly.
Western blot analysis of HDAC4 expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Western blot analysis of extracts of various cell lines, using HDAC4 antibody.
Immunohistochemistry of paraffin-embedded H-lung using HDAC4 Antibody .
Immunohistochemistry of paraffin-embedded human liver cancer using HDAC4 antibody at dilution of 1:200 (200x lens).
Immunohistochemistry of paraffin-embedded mouse liver using HDAC4 antibody at dilution of 1:200 (200x lens).
Immunohistochemistry of paraffin-embedded rat kidney using HDAC4 antibody at dilution of 1:200 (200x lens).
Immunohistochemistry of paraffin-embedded rat lung using HDAC4 antibody at dilution of 1:200 (200x lens).
Immunofluorescence analysis of A549 cell using HDAC4 antibody. Blue: DAPI for nuclear staining.
Western blot analysis of extracts from 293 cells untreated or treated with EGF using Phospho-HDAC4/5/9-S246/259/220 Antibody.
NIH 3T3 (left lane) and Jurkat (right lane) nuclear extracts were probed with rabbit anti-HDAC4 antibody by Western Blot.
Fixed and permeabilized Hela cells were stained with a 1:50 dilution of HDAC4 antibody (Poly6077) for 1 hr, incubated with goat anti-rabbit conjugated to Alexa Fluor® 488 (top panel). Cells were examined at 100 X magnification. DAPI stained cells are shown as a control (bottom panel).
WB Suggested Anti-Hdac4 Antibody; Titration: 1.0 ug/ml; Positive Control: Mouse Liver
Lanes: Lane 1: 40ug mouse brain, synaptosome lysate; Lane 2: 40ug mouse brain, membrane fraction; Lane 3: 40ug mouse brain, cytoplasm fraction; Lane 4: 40ug mouse brain, nuclear fraction; Lane 5: 40ug mouse brain, post synaptic density fraction; Lane 6: 4
Chromatin Immunoprecipitation (ChIP) Using Hdac4 Antibody - C-terminal region and HCT116 Cells
Western blot detection of HDAC4 in Jurkat,Hela and K562 cell lysates using HDAC4 mouse mAb (1:1000 diluted).Predicted band size: 140KDa.Observed band size: 140KDa.
Immunoprecipitation analysis of Hela cell lysates using HDAC4 mouse mAb.
NIH 3T3 (left lane) and Jurkat (right lane) nuclear extracts were probed with rabbit anti-HDAC4 antibody by Western Blot.
Fixed and permeabilized Hela cells were stained with a 1:50 dilution of HDAC4 antibody (Poly6077) for 1 hr, incubated with goat anti-rabbit conjugated to Alexa Fluorreg; 488 (top panel). Cells were examined at 100 X magnification. DAPI stained cells are shown as a control (bottom panel).
Predicted band size: 124; 140 kDa . Positive control: 293 cells untreated or treated with EGF lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1: 293 cells untreated with EGF lysate Lane2: 293 cells treated with EGF lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute)
Predicted cell location: Nucleus. Positive control: Human breast carcinoma tissue. Recommended dilution: 1/ 50-100 The image on the left is immunohistochemistry of paraffin-embedded human breast carcinoma tissue using HDAC4/HDAC5/HDAC9 (phospho-Ser246/259/220) antibody at dilution 1/50, on the right is treated with the synthetic peptide. (Original magnification: 200)
Western blot analysis of extracts from 293 cells and Jurkat cells,using HDAC4 (phospho-Ser632) antibody.The lane on the right is treated with the synthesized peptide.
Western blot analysis of extracts from JK cells (Lane 2), using HDAC4 (Phospho-Ser632) Antibody. The lane on the left is treated with synthesized peptide.
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Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA326791 | HDAC4 antibody |
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| HDAC4 Rabbit Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€325.00
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| OriGene Technologies, Inc. | |||||||
 +6 additional images |
Highly validated antibody! |
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| TA312828 | HDAC4 antibody |
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| Rabbit polyclonal HDAC4 (Ab-632) antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA312829 | HDAC4 antibody |
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| Rabbit polyclonal HDAC4 (Ser632) antibody(Phospho-specific) | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA322533 | HDAC4 antibody |
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| Anti-HDAC4/HDAC5/HDAC9 (phospho-Ser246/259/220) Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | P, WB |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA322534 | HDAC4 antibody |
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| Anti-HDAC4 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA322535 | HDAC4 antibody |
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| Anti-HDAC4 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | E, P |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA324523 | HDAC4 antibody |
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| Rabbit polyclonal HDAC4 Antibody (C-term) | |||||||
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Rabbit | IgG | Purified | Hu | ICC/IF, P, WB |
0.4 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA325515 | HDAC4 antibody |
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| Rabbit Polyclonal HDAC4 Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA325516 | HDAC4 antibody |
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| Rabbit Polyclonal HDAC4 (Ser632) Antibody (Phospho-specific) | |||||||
 |
Rabbit | IgG | Purified | Hu, Ms, Rt | WB |
0.1 ml /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA327587 | HDAC4 antibody |
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| Phospho-HDAC4/5/9-S246/259/220 Rabbit Polyclonal Antibody | |||||||
 |
Rabbit | IgG | Purified | Hu | WB |
0.1 mg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA328016 | HDAC4 antibody |
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| Rabbit Polyclonal anti-HDAC4 Antibody | |||||||
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Rabbit | IgG | Poly6077 | Purified | Hu, Ms | ICC/IF, WB |
0.2 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA332046 | HDAC4 antibody |
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| Rabbit Polyclonal Anti-Hdac4 Antibody | |||||||
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Rabbit | IgG | Purified | Bov, Can, Eq, GP, Hu, Ms, Rt, Ye, Ze | ChIP, WB |
50 µg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| +2 additional images |
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| TA333209 | HDAC4 antibody |
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| Phospho-HDAC4-S632 Rabbit Polyclonal Antibody | |||||||
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Rabbit | IgG | Purified | Hu, Ms, Rt |
0.1 mg /
€325.00
|
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| OriGene Technologies, Inc. | |||||||
| TA346963 | HDAC4 antibody |
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| Mouse Monoclonal HDAC4 (N-terminus) Antibody | |||||||
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Mouse | IgG2a | 4A3-H7-C6 | Purified | WB |
0.1 ml /
€325.00
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA352612 | HDAC4 antibody |
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| Rabbit Polyclonal anti-HDAC4 Antibody | |||||||
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Rabbit | Poly6077 | Purified | Hu, Ms | ICC/IF, WB |
50 µl /
€248.00
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| OriGene Technologies, Inc. | |||||||
| +1 additional image |
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| TA590588 | HDAC4 antibody |
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| Rabbit Polyclonal HDAC4 Antibody | |||||||
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Rabbit | IgG | Purified | Hu | E |
50 µg /
€325.00
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| OriGene Technologies, Inc. | |||||||
| TA804962 | HDAC4 antibody |
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| HDAC4 mouse monoclonal antibody, clone OTI1E1 (formerly 1E1) | |||||||
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Mouse | IgG1 | OTI1E1 | Purified | Hu | WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
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| TA804963 | HDAC4 antibody |
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| HDAC4 mouse monoclonal antibody, clone OTI1C8 (formerly 1C8) | |||||||
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Mouse | IgG1 | OTI1C8 | Purified | Hu | WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
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| TA805008 | HDAC4 antibody |
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| HDAC4 mouse monoclonal antibody, clone OTI1E12 (formerly 1E12) | |||||||
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Mouse | IgG1 | OTI1E12 | Purified | Hu | WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
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| TA805009 | HDAC4 antibody |
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| HDAC4 mouse monoclonal antibody, clone OTI1A12 (formerly 1A12) | |||||||
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Mouse | IgG1 | OTI1A12 | Purified | Hu | WB |
0.1 ml /
€325.00
|
| OriGene Technologies, Inc. | |||||||
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