HDAC4 antibody

Principal name

HDAC4 antibody

Alternative names for HDAC4 antibody

Histone deacetylase 4, HD4, KIAA0288

SwissProt ID

P56524 (Human), P83038 (Chick), Q6NZM9 (Mouse), Q99P99 (Rat)

Gene ID

9759 (HDAC4)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Chk (Chicken), Mky (Monkey), Ze (Zebrafish), African clawed frog, Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Ye (Yeast)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Enzyme Immunoassay (E), ChIP assay (ChIP), Paraffin Sections (P), ELISA (detection) (E(detection))

Background of HDAC4 antibody

Eukaryotic chromatin is composed of highly packed chromosomal DNA that is stabilized by many nuclear proteins. The chromatin DNA is constantly packed and unpacked during gene expression and is controlled a host of proteins. The acetylation of lysine residues in histones, and/or of transcription factors, is an important posttranslational mechanism of transcriptional regulation. The acetylated histone protein produced confirmation changes that confer accessibility of the homology to yeast sequences DNA template to the transcriptional machinery for protein expression. During damage to double stranded DNA, a number of proteins are recruited to nuclear foci including HDAC4, 53BP1 and Rad51 proteins. The HDAC4 gradually disappear from foci in repair proficient cells but linger on for some time in repair deficient cells suggesting that hDCA4 is linked to DNA repair (1). Silencing CA4 by SiRNA also resulted in decrease level of partner protein 53BP1 and abrogated DNA damage-induced G2 delay in radio sensitized HeLa cells (1). Nucleocytoplasmic trafficking is an important aspect of HDAC4 functioning, and binding of 14-3-3 proteins is necessary for its cytoplasmic retention. The N-terminal 118 amino acids modulate the nuclear localization but residues 244-279 constitute a strong nuclear localization signal. A hydrophobic motif located at C-terminus serves as nuclear export signal that is necessary for cytoplasmic retentionof HDAC4 (2).
Histone deacetylases (HDACs) catalyze the deacetylation of histone and non-histone substrates, and in general act to repress transcription as part of larger corepressor complexes. Eighteen mammalian HDACs have been identified to date, and they are grouped into four classes based on their respective homology to yeast deacetylases (3). The class II HDACs can be further subdivided into class IIa (HDAC4, -5, -7, and -9) and class IIb (HDAC6 and -10), based on the presence in class IIa members of conserved motifs in the N-terminal domain, and extended C terminal tails, that are essential in regulating their function (4). The HDCA4 is a transcriptional corepressor that exist in distinct corepressor complex than class I proteins and it shuttles between nucleus and cytoplasmic compartment involving active nuclear transport. In the nucleus, HDAC4 associates with the myocyte enhancer factor MEF2A. Binding of HDAC4 to MEF2A results in the repression of MEF2A transcriptional activation. HDAC4 has also been shown to interact with other deacetylases such as HDAC3 as well as the corepressors NcoR and SMART. HDCA4 has a direct role in proproliferation of colon cancer by Sp1-dependent recruitment of HDCA4 to the p21 promotor and acting in concert with HDAC4-HDCA3-N-CoR/SMART co repressor complex resulting in transcriptional repression (5).

General readings

General / background references:Fischle W et al. The emerging role of class II histone deacetylases. Biochem Cell Biol 79:337-48 (2001). PubMed PMID: 11467747
Khochbin S et al. Functional significance of histone deacetylase diversity. Curr Opin Genet Dev 11:162-6 (2001). PubMed PMID: 11250139
Wang AH et al. HDAC4, a human histone deacetylase related to yeast HDA1, is a transcriptional corepressor. Mol Cell Biol 19:7816-27 (1999). PubMed PMID: 10523670
Grozinger CM et al. Three proteins define a class of human histone deacetylases related to yeast Hda1p. Proc Natl Acad Sci U S A 96:4868-73 (1999). PubMed PMID: 10220385
1. Geng, L., Cuneo, K. C., Fu, A., Tu, T., Atadja, P. W., and Hallahan, D. E. (2006) Histone Deacetylase (HDAC) Inhibitor LBH589 Increases Duration of {gamma}-H2AX Foci and Confines HDAC4 to the Cytoplasm in Irradiated Non-Small Cell Lung Cancer, 66, 11298-11304.

  1. Quick-Filters:
  2. Cited (1)
  3. Highly Validated (1)

Items 1 to 20 of 64 total

per page
Page:
  1. 1
  2. 2
  3. 3
  4. 4

Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

HDAC4 antibody

Western blot analysis of extracts of various cell lines, using HDAC4 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

HDAC4 antibody

Western blot analysis of extracts from HeLa/Jurkat cells, using HDAC4 (TA312828) Antibody. The lane on the right is treated with the synthesized peptide Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HDAC4 antibody

Predicted cell location: Cytoplasm. Positive control: Human tonsil tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using HDAC4 antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

HDAC4 antibody

Predicted cell location: Cytoplasm. Positive control: Human tonsil tissue. Recommended dilution: 1/10-50  The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using HDAC4 antibody at dilution 1/15, on the right is treated with the synthetic peptide. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

HDAC4 antibody

HDAC4 Antibody (C-term) (Cat. #TA324523) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the HDAC4 antibody detected the HDAC4 protein (arrow). Rabbit IgG Purified Hu ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

HDAC4 antibody

Western blot analysis of HDAC4 expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HDAC4 antibody

Western blot analysis of extracts from 293 cells untreated or treated with EGF using Phospho-HDAC4/5/9-S246/259/220 Antibody. Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HDAC4 antibody

NIH 3T3 (left lane) and Jurkat (right lane) nuclear extracts were probed with rabbit anti-HDAC4 antibody by Western Blot. Rabbit IgG Poly6077 Purified Hu, Ms ICC/IF, WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

HDAC4 antibody

WB Suggested Anti-Hdac4 Antibody; Titration: 1.0 ug/ml; Positive Control: Mouse Liver Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rt, Ye, Ze ChIP, WB
50 µg / €325.00
  OriGene Technologies, Inc.

HDAC4 antibody

Western blot detection of HDAC4 in Jurkat,Hela and K562 cell lysates using HDAC4 mouse mAb (1:1000 diluted).Predicted band size: 140KDa.Observed band size: 140KDa. Mouse IgG2a 4A3-H7-C6 Purified WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HDAC4 antibody

ELISA: HDAC4 Antibody Rabbit IgG Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

HDAC4 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC4 (RC211495, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC4. Mouse IgG1 OTI1E1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HDAC4 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC4 (RC211495, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC4. Mouse IgG1 OTI1C8 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HDAC4 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC4 (RC211495, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC4. Mouse IgG1 OTI1E12 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HDAC4 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC4 (RC211495, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC4. Mouse IgG1 OTI1A12 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HDAC4 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC4 (RC211495, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC4. Mouse IgG1 OTI4A4 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HDAC4 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC4 (RC211495, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC4. Mouse IgG1 OTI6E6 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HDAC4 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC4 (RC211495, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC4. Mouse IgG1 OTI7E1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HDAC4 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC4 (RC211495, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC4. Mouse IgG1 OTI4D7 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HDAC4 antibody

Western blot analysis using HDAC4 mouse mAb against Hela (1), Jurkat (2) cell lysate. Mouse IgG1 7B2 Ascites Hu E, WB
0.1 ml / €400.00
  Acris Antibodies GmbH

Items 1 to 20 of 64 total

per page
Page:
  1. 1
  2. 2
  3. 3
  4. 4
  • LinkedIn