HDAC4 antibody

Principal name

HDAC4 antibody

Alternative names for HDAC4 antibody

Histone deacetylase 4, HD4, KIAA0288

SwissProt ID

P56524 (Human), P83038 (Chick), Q6NZM9 (Mouse), Q99P99 (Rat)

Gene ID

9759 (HDAC4), 208727 (Hdac4)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Chk (Chicken), Mky (Monkey), Ze (Zebrafish), African clawed frog, Bov (Bovine), Can (Canine), Eq (Equine), GP (Guinea Pig), Ye (Yeast)

Available hosts

Rabbit, Mouse

Available applications

Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF), Enzyme Immunoassay (E), Paraffin Sections (P), ChIP assay (ChIP), Immunoprecipitation (IP), ELISA (detection) (E(detection))

Background of HDAC4 antibody

Eukaryotic chromatin is composed of highly packed chromosomal DNA that is stabilized by many nuclear proteins. The chromatin DNA is constantly packed and unpacked during gene expression and is controlled a host of proteins. The acetylation of lysine residues in histones, and/or of transcription factors, is an important posttranslational mechanism of transcriptional regulation. The acetylated histone protein produced confirmation changes that confer accessibility of the homology to yeast sequences DNA template to the transcriptional machinery for protein expression. During damage to double stranded DNA, a number of proteins are recruited to nuclear foci including HDAC4, 53BP1 and Rad51 proteins. The HDAC4 gradually disappear from foci in repair proficient cells but linger on for some time in repair deficient cells suggesting that hDCA4 is linked to DNA repair (1). Silencing CA4 by SiRNA also resulted in decrease level of partner protein 53BP1 and abrogated DNA damage-induced G2 delay in radio sensitized HeLa cells (1). Nucleocytoplasmic trafficking is an important aspect of HDAC4 functioning, and binding of 14-3-3 proteins is necessary for its cytoplasmic retention. The N-terminal 118 amino acids modulate the nuclear localization but residues 244-279 constitute a strong nuclear localization signal. A hydrophobic motif located at C-terminus serves as nuclear export signal that is necessary for cytoplasmic retentionof HDAC4 (2).
Histone deacetylases (HDACs) catalyze the deacetylation of histone and non-histone substrates, and in general act to repress transcription as part of larger corepressor complexes. Eighteen mammalian HDACs have been identified to date, and they are grouped into four classes based on their respective homology to yeast deacetylases (3). The class II HDACs can be further subdivided into class IIa (HDAC4, -5, -7, and -9) and class IIb (HDAC6 and -10), based on the presence in class IIa members of conserved motifs in the N-terminal domain, and extended C terminal tails, that are essential in regulating their function (4). The HDCA4 is a transcriptional corepressor that exist in distinct corepressor complex than class I proteins and it shuttles between nucleus and cytoplasmic compartment involving active nuclear transport. In the nucleus, HDAC4 associates with the myocyte enhancer factor MEF2A. Binding of HDAC4 to MEF2A results in the repression of MEF2A transcriptional activation. HDAC4 has also been shown to interact with other deacetylases such as HDAC3 as well as the corepressors NcoR and SMART. HDCA4 has a direct role in proproliferation of colon cancer by Sp1-dependent recruitment of HDCA4 to the p21 promotor and acting in concert with HDAC4-HDCA3-N-CoR/SMART co repressor complex resulting in transcriptional repression (5).

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

HDAC4 antibody

  HDAC4 Rabbit Polyclonal Antibody  
Western blot analysis of extracts of various cell lines, using HDAC4 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

HDAC4 antibody

  Rabbit polyclonal HDAC4 (Ab-632) antibody  
Western blot analysis of extracts from HeLa/Jurkat cells, using HDAC4 (TA312828) Antibody. The lane on the right is treated with the synthesized peptide Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HDAC4 antibody

  Rabbit polyclonal HDAC4 (Ser632) antibody(Phospho-specific)  
Western blot analysis of extracts from 293 cells and Jurkat cells,using HDAC4 (phospho-Ser632) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HDAC4 antibody

  Anti-HDAC4/HDAC5/HDAC9 (phospho-Ser246/259/220) Rabbit Polyclonal Antibody  
Predicted band size: 124; 140 kDa . Positive control: 293 cells untreated or treated with EGF lysate. Recommended dilution: 1/ 500-1000. (Gel: 8%SDS-PAGE Lane1: 293 cells untreated with EGF lysate Lane2: 293 cells treated with EGF lysate Lysates: 30 ug per lane Primary antibody: 1/500 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 1 minute) Rabbit IgG Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HDAC4 antibody

  Anti-HDAC4 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human tonsil tissue. Recommended dilution: 1/25-100  The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using HDAC4 antibody at dilution 1/25, on the right is treated with the synthetic peptide. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

HDAC4 antibody

  Anti-HDAC4 Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human tonsil tissue. Recommended dilution: 1/10-50  The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using HDAC4 antibody at dilution 1/15, on the right is treated with the synthetic peptide. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

HDAC4 antibody

  Rabbit polyclonal HDAC4 Antibody (C-term)  
HDAC4 Antibody (C-term) (Cat. #TA324523) western blot analysis in NCI-H292 cell line lysates (35ug/lane).This demonstrates the HDAC4 antibody detected the HDAC4 protein (arrow). Rabbit IgG Purified Hu ICC/IF, P, WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

HDAC4 antibody

  Rabbit Polyclonal HDAC4 Antibody  
Western blot analysis of HDAC4 expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HDAC4 antibody

  Rabbit Polyclonal HDAC4 (Ser632) Antibody (Phospho-specific)  
HDAC4 Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HDAC4 antibody

  Phospho-HDAC4/5/9-S246/259/220 Rabbit Polyclonal Antibody  
Western blot analysis of extracts from 293 cells untreated or treated with EGF using Phospho-HDAC4/5/9-S246/259/220 Antibody. Rabbit IgG Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HDAC4 antibody

  Rabbit Polyclonal anti-HDAC4 Antibody  
NIH 3T3 (left lane) and Jurkat (right lane) nuclear extracts were probed with rabbit anti-HDAC4 antibody by Western Blot. Rabbit IgG Poly6077 Purified Hu, Ms ICC/IF, WB
0.2 ml / €325.00
  OriGene Technologies, Inc.

HDAC4 antibody

  Rabbit Polyclonal Anti-Hdac4 Antibody  
WB Suggested Anti-Hdac4 Antibody; Titration: 1.0 ug/ml; Positive Control: Mouse Liver Rabbit IgG Purified Bov, Can, Eq, GP, Hu, Ms, Rt, Ye, Ze ChIP, WB
50 µg / €325.00
  OriGene Technologies, Inc.

HDAC4 antibody

  Phospho-HDAC4-S632 Rabbit Polyclonal Antibody  
HDAC4 Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

HDAC4 antibody

  Mouse Monoclonal HDAC4 (N-terminus) Antibody  
Western blot detection of HDAC4 in Jurkat,Hela and K562 cell lysates using HDAC4 mouse mAb (1:1000 diluted).Predicted band size: 140KDa.Observed band size: 140KDa. Mouse IgG2a 4A3-H7-C6 Purified WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HDAC4 antibody

  Rabbit Polyclonal anti-HDAC4 Antibody  
NIH 3T3 (left lane) and Jurkat (right lane) nuclear extracts were probed with rabbit anti-HDAC4 antibody by Western Blot. Rabbit Poly6077 Purified Hu, Ms ICC/IF, WB
50 µl / €248.00
  OriGene Technologies, Inc.

HDAC4 antibody

  Rabbit Polyclonal HDAC4 Antibody  
ELISA: HDAC4 Antibody Rabbit IgG Purified Hu E
50 µg / €325.00
  OriGene Technologies, Inc.

HDAC4 antibody

  HDAC4 mouse monoclonal antibody, clone OTI1E1 (formerly 1E1)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC4 (RC211495, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC4. Mouse IgG1 OTI1E1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HDAC4 antibody

  HDAC4 mouse monoclonal antibody, clone OTI1C8 (formerly 1C8)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC4 (RC211495, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC4. Mouse IgG1 OTI1C8 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HDAC4 antibody

  HDAC4 mouse monoclonal antibody, clone OTI1E12 (formerly 1E12)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC4 (RC211495, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC4. Mouse IgG1 OTI1E12 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

HDAC4 antibody

  HDAC4 mouse monoclonal antibody, clone OTI1A12 (formerly 1A12)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HDAC4 (RC211495, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HDAC4. Mouse IgG1 OTI1A12 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

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