HDAC8 antibody
Principal name
HDAC8 antibody
Alternative names for HDAC8 antibody
Histone deacetylase 8, HD8, HDACL1, CDA07
Ncbi ID
NP_001159890.1, 9606, NP_060956, 10090, NP_060956.1, NM_018486, NM_001166418, NP_081658.1, NM_027382, NM_001166448, NM_001166420, NM_001166419
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), Paraffin Sections (P), ELISA (detection) (E(detection)), Frozen Sections (C), Immunoprecipitation (IP)
Background of HDAC8 antibody
In the intact cell, DNA closely associates with histones and other nuclear proteins to form chromatin. The remodeling of chromatin is believed to be a critical component of transcriptional regulation and a major source of this remodeling is brought about by the acetylation of nucleosomal histones. Acetylation of lysine residues in the amino terminal tail domain of histone results in an allosteric change in the nucleosomal conformation and an increased accessibility to transcription factors by DNA. Conversely, the deacetylation of histones is associated with transcriptional silencing. Several mammalian proteins have been identified as nuclear histone acetylases, including GCN5, PCAF (p300/CBP-associated factor), p300/CBP, HAT1 and the TFIID subunit TAF II p250. Mammalian HDAC8, isolated from human kidney, is a histone deacetylase that shares homology to other HDACs but has different tissue distribution. HDAC8 is localized to the nucleus and plays a role in the development of a broad range of tissues and in the etiology of cancer.
General readings
Hu E., et al. (2000) J. Biol. Chem. 275:15254-15264Buggy J.J., et al. (2000) Biochem. J. 350:199-205
Immunofluorescence of monoclonal antibody to HDAC8 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of HDAC8 expression in transfected 293T cell line by HDAC8 monoclonal antibody (M01), clone 2F4.Lane 1: HDAC8 transfected lysate(41.8 KDa).Lane 2: Non-transfected lysate.
HDAC8 monoclonal antibody (M01), clone 2F4 Western Blot analysis of HDAC8 expression in Hela NE ( Cat # L013V3 ).
Detection limit for recombinant GST tagged HDAC8 is approximately 0.3ng/ml as a capture antibody.
Human Jurkat; WB Suggested Anti-HDAC8 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Jurkat cell lysate; HDAC8 antibody - C-terminal region (ARP34587_P050) in Human Jurkat cells using Western Blot
Immunohistochemistry (IHC) analyzes of HDAC8 antibody (Cat.-No.: AP06162PU-N) in paraffin-embedded human lung carcinoma tissue.
Western blot (WB) analysis of HDAC8 antibody (Cat.-No.: AP06162PU-N) in extracts from raw264.7 cells.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the HDAC8 polyclonal antibody ( Cat # PAB2336 ) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry ; clinical relevance has not been evaluated. HC = hepatocarcinoma.
Western blot analysis of HDAC8 polyclonal antibody ( Cat # PAB2336 ) in mouse NIH/3T3 cell lysate. HDAC8 ( Arrow ) was detected using purified polyclonal antibody. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Western blot analysis of extracts from NIH/3T3 cells using HDAC8 polyclonal antibody ( Cat # PAB5387 ) .
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HDAC8 polyclonal antibody ( Cat # PAB5387 ) .
Immunofluorescence of purified MaxPab antibody to HDAC8 on HeLa cell. [antibody concentration 10 ug/ml]
Western Blot analysis of HDAC8 expression in transfected 293T cell line (H00055869-T01) by HDAC8 MaxPab polyclonal antibody.Lane 1: HDAC8 transfected lysate(41.47 KDa).Lane 2: Non-transfected lysate.
Immunohistochemistry (IHC) analysis of HDAC8 pSer39 antibody in paraffin-embedded human lung carcinoma tissue.
Western blot (WB) analysis of HDAC8 pSer39 antibody in extracts from NIH/3T3 cells.
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of anti-HDAC8 Pab (Cat. #AP11136PU-N) in mouse 3T3 cell lysate. HDAC8 (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.
Western Blot analysis of HDAC8 expression in transfected 293T cell line (H00055869-T01) by HDAC8 MaxPab polyclonal antibody.Lane 1: HDAC8 transfected lysate(41.80 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of Hdac8 with human Jurkat cell lysates. The antibody recognizes 78 kDa and 42 kDa Hdac8 of human, mouse, and rat origins.
Immunoprecipitation of HDAC8 transfected lysate using anti-HDAC8 MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with HDAC8 MaxPab mouse polyclonal antibody (B01) (H00055869-B01).
Transfected 293T; WB Suggested Anti-HDAC8 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Transfected 293T; HDAC8 antibody - N-terminal region (ARP34586_P050) in Transfected 293T cells using Western Blot
Immunohistochemical analysis of Histone Deacetylase 8 (pS39) staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
Immunofluorescent analysis of Histone Deacetylase 8 (pS39) staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Western blot analysis of Histone Deacetylase 8 (pS39) expression in A549 (A); NIH3T3 (B); rat kidney (C) whole cell lysates.
Western blot analysis of extracts from NIH/3T3 cells using HDAC8 antibody.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using HDAC8 antibody.
Western blot analysis of HDAC8 with human Jurkat cell lysates
Western blot analysis using recombinant human HDAC8. Lane 1: 30 ng, Lane 2: 60 ng, Lane 3: 120 ng, Lane 4: 250 ng.
Western blot analysis of HDAC8 expression in HeLa whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Western blot analysis of extracts of various cell lines, using HDAC8 antibody.
WB Suggested Anti-HDAC8 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Transfected 293T
WB Suggested Anti-HDAC8 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:1562500; Positive Control: Jurkat cell lysate
Gel: 8%SDS-PAGE, Lysate: 40 ug, Lane: 293T cells, Primary antibody: (HDAC8 Antibody) at dilution 1/950, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 3 seconds
The image on the left is immunohistochemistry of paraffin-embedded Human tonsil tissue using (HDAC8 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200)
The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using (HDAC8 Antibody) at dilution 1/40, on the right is treated with fusion protein. (Original magnification: ×200)
Western blot analysis of HDAC8 phosphorylation expression in NIH-3T3 whole cell lysates,The lane on the left is treated with the antigen-specific peptide.
Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue using HDAC8 (phospho-Ser39) antibody.
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA337234 | HDAC8 antibody |
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| Rabbit Polyclonal Anti-HDAC8 Antibody | |||||||
 |
Rabbit | IgG | Purified | Bov, Can, Eq, GP, Hu, Ms, Rb, Rt | WB |
50 µg /
€360.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA337235 | HDAC8 antibody |
||||||
| Rabbit Polyclonal Anti-HDAC8 Antibody | |||||||
 |
Rabbit | IgG | Purified | Bov, Can, Eq, GP, Hu, Ms, Por, Rb, Rt, Ze | WB |
50 µg /
€360.00
|
|
| OriGene Technologies, Inc. | |||||||
