HDDC3 antibody
Principal name
HDDC3 antibody
Alternative names for HDDC3 antibody
HD domain-containing protein 3
Ncbi ID
Available reactivities
Available hosts
Available applications
Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Paraffin Sections (P)
Background of HDDC3 antibody
HDDC3, also known as guanosine-3',5'-bis(diphosphate)-pyrophosphohydrolase MESH1, contains an active site for ppGpp hydrolysis and a conserved His-Asp-box motif for Mn(2+) binding. Consistent with these structural data, HDDC3 efficiently catalyzes hydrolysis of guanosine 3',5'-diphosphate (ppGpp) both in vitro and in vivo. HDDC3 also suppresses SpoT-deficient lethality and RelA-induced delayed cell growth in bacteria.
General readings
Sun D., et al. (2010) Nat. Struct. Mol. Biol. 17:1188-1194Zody M.C., et al. (2006) Nature. 440:671-675
Human HeLa; WB Suggested Anti-HDDC3 Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:312500. Positive Control: Hela cell lysate; HDDC3 antibody - middle region (ARP55898_P050) in Human HeLa cells using Western Blot
Immunohistochemical of paraffin-embedded human kidney using 21091-1-AP(HDDC3 antibody) at dilution of 1:100 (under 40x lens)
Immunohistochemical of paraffin-embedded human kidney using 21091-1-AP(HDDC3 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using 21091-1-AP(HDDC3 antibody) at dilution of 1:100 (under 10x lens)
Immunohistochemical of paraffin-embedded human brain using 21091-1-AP(HDDC3 antibody) at dilution of 1:100 (under 40x lens)
Mouse heart tissue were subjected to SDS PAGE followed by western blot with 21091-1-AP(HDDC3 antibody) at dilution of 1:600
Mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21091-1-AP(HDDC3 antibody) at dilution of 1:600
HeLa cells were subjected to SDS PAGE followed by western blot with 21091-1-AP(HDDC3 antibody) at dilution of 1:500
Mouse testis tissue were subjected to SDS PAGE followed by western blot with 21091-1-AP(HDDC3 antibody) at dilution of 1:800
Mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 21091-1-AP(HDDC3 antibody) at dilution of 1:500
Mouse lung tissue were subjected to SDS PAGE followed by western blot with 21091-1-AP(HDDC3 antibody) at dilution of 1:500
Human heart tissue were subjected to SDS PAGE followed by western blot with 21091-1-AP(HDDC3 antibody) at dilution of 1:500
Western Blot: HDDC3 Antibody [NBP1-56806] - This Anti-HDDC3 antibody was used in Western Blot of Hela tissue lysate at a concentration of 1ug/ml.
Immunohistochemistry-Paraffin: HDDC3 Antibody [NBP1-82684] - Immunohistochemical staining of human stomach, lower shows strong cytoplasmic and nuclear positivity in glandular cells.
Western Blot: HDDC3 Antibody [NBP1-82684] Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10 | Lane 2: RT-4 | Lane 3: U-251 MG | Lane 4: Human Plasma | Lane 5: Liver | Lane 6: Tonsil
Detection of HDDC3 in Hela using anti-HDDC3 antibody (GTX45162) at 1 µg/mL.
WB suggested Anti-HDDC3 Antibody Titration: 0.2-1 ug/mL; ELISA Titer: 1:312500; Positive Control: HeLa cell lysate
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA333355 | HDDC3 antibody |
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 |
Rabbit | IgG | Purified | Bov, Can, Hu, Por, Rt, Ze | WB |
50 µg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| TA334094 | HDDC3 antibody |
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 |
Rabbit | IgG | Purified | Hu, Rt | WB |
50 µg /
€325.00
|
|
| OriGene Technologies, Inc. | |||||||
| H00374659-M05 | HDDC3 antibody |
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 |
Mouse | IgG2a | 7E6 | Purified | Hu | E, WB |
0.1 mg /
€370.00
|
| Abnova Taiwan Corp. | |||||||
| ARP55898_P050 | HDDC3 antibody |
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 |
Rabbit | Aff - Purified | African clawed frog, Can, Hu, Ms, Rt, Ze | WB |
50 µg /
€350.00
|
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| AVIVA Systems Biology | |||||||
| NBP1-56806 | HDDC3 antibody |
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![Western Blot: HDDC3 Antibody [NBP1-56806] - This Anti-HDDC3 antibody was used in Western Blot of Hela tissue lysate at a concentration of 1ug/ml.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/HDDC3 Antibody-Western Blot-NBP1-56806-img0001.jpg) |
Rabbit | Aff - Purified | Hu, Ms | WB |
50 µg /
€440.00
|
||
| Novus Biologicals Inc. | |||||||
| NBP1-82684 | HDDC3 antibody |
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![Immunohistochemistry-Paraffin: HDDC3 Antibody [NBP1-82684] - Immunohistochemical staining of human stomach, lower shows strong cytoplasmic and nuclear positivity in glandular cells.](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/Novus/HDDC3 Antibody-Immunohistochemistry-Paraffin-NBP1-82684-0005.jpg) |
Rabbit | IgG | Aff - Purified | Hu | P, WB |
0.1 ml /
€500.00
|
|
| Novus Biologicals Inc. | |||||||
 +1 additional image |
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Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP307636 | HDDC3 |
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 |
Human | > 80 % Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps. Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
HEK293 cells |
20 µg /
€680.00
|
|
| OriGene Technologies, Inc. | |||||
| AR50092PU-N | HDDC3 (1-140, His-tag) |
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 |
Human | Purified | > 90 % by SDS - PAGE | E. coli |
0.5 mg /
€730.00
|
| Acris Antibodies GmbH | |||||
| AR50092PU-S | HDDC3 (1-140, His-tag) |
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|
Human | Purified | > 90 % by SDS - PAGE | E. coli |
0.1 mg /
€300.00
|
| Acris Antibodies GmbH | |||||
| H00374659-P01-10 | HDDC3 |
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|
Human | Purified | 12.5% SDS-PAGE Stained with Coomassie Blue. |
10 µg /
€380.00
|
|
| Abnova Taiwan Corp. | |||||
| H00374659-P01-25 | HDDC3 |
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|
Human | Purified | 12.5% SDS-PAGE Stained with Coomassie Blue. |
25 µg /
€510.00
|
|
| Abnova Taiwan Corp. | |||||
Lysates (Overexpressed)
| Catalog No. | ||
|---|---|---|
| LY404868 | HDDC3 overexpression lysate |
|
|
0.1 mg /
€280.00
|
|
| OriGene Technologies, Inc. |
