HDGFRP3 antibody
Principal name
HDGFRP3 antibody
Alternative names for HDGFRP3 antibody
HRP-3, HDGF2, CGI-142, Hepatoma-derived growth factor-related protein 3, Hepatoma-derived growth factor 2
Gene ID
50810 (HDGFRP3)
Ncbi ID
Available reactivities
Available hosts
Available applications
Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Paraffin Sections (P)
Background of HDGFRP3 antibody
Hepatoma-derived growth factor, related protein 3, also known as HDGFRP3, is the original member of a family of polypeptides designated HDGF related proteins (HRPs). This protein enhances DNA synthesis and may play a role in cell proliferation. HDGFRP3 is expressed predominantly in the testis and brain, to an intermediate extent in the heart, and to a slight extent in the ovaries, kidneys, spleen, and liver in humans.
General readings
Ikegame K, Yamamoto M, Kishima Y, et al. (2000). "A new member of a hepatoma-derived growth factor gene family can translocate to the nucleus". Biochem. Biophys. Res. Commun. 266 (1): 81–7.
Immunofluorescence of monoclonal antibody to HDGFRP3 on HeLa cell . [antibody concentration 10 ug/ml]
Western Blot analysis of HDGFRP3 expression in transfected 293T cell line by HDGFRP3 monoclonal antibody (M01), clone 2G12.Lane 1: HDGFRP3 transfected lysate(23 KDa).Lane 2: Non-transfected lysate.
Detection limit for recombinant GST tagged HDGFRP3 is approximately 0.1ng/ml as a capture antibody.
Western Blot analysis of HDGFRP3 expression in transfected 293T cell line (H00050810-T01) by HDGFRP3 MaxPab polyclonal antibody.Lane 1: HDGFRP3 transfected lysate(22.33 KDa).Lane 2: Non-transfected lysate.
Western Blot analysis of HDGFRP3 expression in transfected 293T cell line (H00050810-T02) by HDGFRP3 MaxPab polyclonal antibody.Lane 1: HDGFRP3 transfected lysate(22.60 KDa).Lane 2: Non-transfected lysate.
Western Blot: HDGFRP3 Lysate [NBL1-11490] - Western Blot experiments. Left-Control; Right -Over-expression Lysate for HDGFRP3
Immunohistochemical of paraffin-embedded human prostate cancer using 12380-1-AP(HDGFRP3 antibody) at dilution of 1:50 (under 10x lens)
Immunofluorescent analysis of MCF-7 cells, using HDGFRP3 antibody 12380-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Human brain tissue were subjected to SDS PAGE followed by western blot with 12380-1-AP(HDGFRP3 antibody) at dilution of 1:500
Y79 cells were subjected to SDS PAGE followed by western blot with 12380-1-AP(HDGFRP3 antibody) at dilution of 1:300
IP Result of anti-HDGFRP3 (IP:12380-1-AP, 3ug; Detection:12380-1-AP 1:300) with mouse testis tissue lysate 4800ug.
Human Spleen; WB Suggested Anti-HDGFRP3 Antibody Titration: 0.2-1 ug/ml. Positive Control: Human Spleen; HDGFRP3 antibody - middle region (ARP56825_P050) in Human Spleen cells using Western Blot
Human Spleen; WB Suggested Anti-HDGFRP3 Antibody Titration: 0.2-1 ug/ml. Positive Control: Human Spleen; HDGFRP3 antibody - middle region (AP46182PU-N) in Human Spleen cells using Western Blot
Primary Antibodies
| Catalog No. | Host | Iso. | Clone | Pres. | React. | Applications | |
|---|---|---|---|---|---|---|---|
| TA345070 | HDGFRP3 (Center) antibody |
||||||
 |
Rabbit | Aff - Purified | Hu | WB |
50 µg /
€325.00
|
||
| OriGene Technologies, Inc. | |||||||
| H00050810-B01 | HDGFRP3 antibody |
||||||
 |
Mouse | Serum | Hu | E(detection), WB |
50 µl /
€370.00
|
||
| Abnova Taiwan Corp. | |||||||
| H00050810-B02P | HDGFRP3 antibody |
||||||
 |
Mouse | Purified | Hu | E(detection), WB |
50 µg /
€370.00
|
||
| Abnova Taiwan Corp. | |||||||
| H00050810-D01P | HDGFRP3 antibody |
||||||
 |
Rabbit | Purified | Hu | E(detection), WB |
0.1 mg /
€380.00
|
||
| Abnova Taiwan Corp. | |||||||
| H00050810-M01 | HDGFRP3 antibody |
||||||
![Immunofluorescence of monoclonal antibody to HDGFRP3 on HeLa cell . [antibody concentration 10 ug/ml]](https://m1.acris-antibodies.com/catalog/product/cache/1/small_image/50x50/9df78eab33525d08d6e5fb8d27136e95/H00050810-M01-4-1-1-L.jpg) |
Mouse | IgG2b | 2G12 | Purified | Hu | E, ICC/IF, WB |
0.1 mg /
€370.00
|
| Abnova Taiwan Corp. | |||||||
 +2 additional images |
|||||||
| H00050810-M02 | HDGFRP3 antibody |
||||||
|
Mouse | IgG2a | 1D1 | Purified | Hu | E, WB |
0.1 mg /
€370.00
|
| Abnova Taiwan Corp. | |||||||
| ARP56825_P050 | HDGFRP3 (Center) antibody |
||||||
 |
Rabbit | Aff - Purified | Hu | WB |
50 µg /
€350.00
|
||
| AVIVA Systems Biology | |||||||
| NBP1-79645 | HDGFRP3 antibody |
||||||
|
Rabbit | Aff - Purified | Hu | WB |
50 µg /
€440.00
|
||
| Novus Biologicals Inc. | |||||||
Proteins & Growth Factors
| Catalog No. | Species | Pres. | Purity | Source | |
|---|---|---|---|---|---|
| TP760165 | HDGFRP3 |
||||
 |
Human | > 80 % Preparation: . Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining. |
E. coli |
50 µg /
€199.00
|
|
| OriGene Technologies, Inc. | |||||
| AR50191PU-N | HDGFRP3 (1-203, His-tag) |
||||
 |
Human | Purified | > 90 % by SDS - PAGE | E. coli |
0.5 mg /
€730.00
|
| Acris Antibodies GmbH | |||||
| AR50191PU-S | HDGFRP3 (1-203, His-tag) |
||||
|
Human | Purified | > 90 % by SDS - PAGE | E. coli |
0.1 mg /
€300.00
|
| Acris Antibodies GmbH | |||||
| H00050810-P01-10 | HDGFRP3 |
||||
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. |
10 µg /
€380.00
|
|
| Abnova Taiwan Corp. | |||||
| H00050810-P01-25 | HDGFRP3 |
||||
|
Human | 12.5% SDS-PAGE Stained with Coomassie Blue. | in vitro transl. |
25 µg /
€510.00
|
|
| Abnova Taiwan Corp. | |||||
Lysates
| Catalog No. | ||
|---|---|---|
| H00050810-T01 | HDGFRP3 293T Cell Transient Overexpression Lysate(Denatured) |
|
|
0.1 ml /
€270.00
|
|
| Abnova Taiwan Corp. | ||
| L125T6 | HDGFRP3 HEK293 Cell Transient Overexpression Lysate (Non-Denatured) |
|
|
0.1 mg /
€270.00
|
|
| Abnova Taiwan Corp. |
