Heat shock factor 1 / HSF1 antibody

Principal name

Heat shock factor 1 / HSF1 antibody

Alternative names for Heat shock factor 1 / HSF1 antibody

HSTF1, HSF-1, Heat shock transcription factor 1

SwissProt ID

P38529 (Chick), P38532 (Mouse), Q00613 (Human), Q08DJ8 (Bovin)

Gene ID

3297 (HSF1), 15499 (Hsf1)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Bov (Bovine), Can (Canine), GP (Guinea Pig), Hst (Hamster), Mky (Monkey), Rb (Rabbit), Av (Birds)

Available hosts

Rabbit, Mouse, Rat

Available applications

Electrophoretic Mobility Shift Assay (EMSA), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Paraffin Sections (P), Flow Cytometry (F), Frozen Sections (C)

Background of Heat shock factor 1 / HSF1 antibody

All organisms respond to elevated temperatures and a variety of environmental stresses by rapid synthesis of heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by the transcriptional activator, heat shock factor (HSF), which binds to heat shock response elements (HSEs). These HSEs are found as three repeats of a 5-nucleotide {nGAAn} module, arranged in alternating orientation and present upstream of all heat shock genes. The HSEs are highly conserved among species yet HSF purified from yeast, Drosophila and human have different molecular weights and the proteins do not show significant immunological cross reaction. Two HSFs have been identified in human cells, HSF 1 and HSF 2, which bind to the same HSEs and have 38% sequence identity. These factors are activated by distinct stimuli, HSF 1 is responsive to classical stress signals such as heat, heavy metals and oxidative reagents, whereas HSF 2 is activated during hemin-mediated differentiation of human erythroleukemia cells. HSF 1 exists constitutively in the cytoplasm and the nucleus of unstressed cells as a monomer which lacks DNA binding activity. Through an unknown signal generated during stress, HSF 1 becomes activated to a nuclear localized, trimeric state which binds to DNA. The phosphorylation of HSF 1 is necessary for maximal transcription of heat shock genes. All organisms respond to elevated temperatures and a variety of environmental stresses by rapid synthesis of heat shock RNAs and proteins. The regulation of heat shock gene transcription is mediated by the transcriptional activator, heat shock factor (HSF), which binds to heat shock response elements (HSEs). These HSEs are found as three repeats of a 5-nucleotide {nGAAn} module, arranged in alternating orientation and present upstream of all heat shock genes. The HSEs are highly conserved among species yet HSF purified from yeast, Drosophila and human have different molecular weights and the proteins do not show significant immunological cross reaction. Two HSFs have been identified in human cells, HSF 1 and HSF 2, which bind to the same HSEs and have 38% sequence identity. These factors are activated by distinct stimuli, HSF 1 is responsive to classical stress signals such as heat, heavy metals and oxidative reagents, whereas HSF 2 is activated during hemin-mediated differentiation of human erythroleukemia cells. HSF 1 exists constitutively in the cytoplasm and the nucleus of unstressed cells as a monomer which lacks DNA binding activity. Through an unknown signal generated during stress, HSF 1 becomes activated to a nuclear localized, trimeric state which binds to DNA. The phosphorylation of HSF 1 is necessary for maximal transcription of heat shock genes.

General readings

This product has been used in:Sun L et al. Activation of HSF and selective increase in heat-shock proteins by acute dexamethasone treatment. Am J Physiol Heart Circ Physiol 278:H1091-7 (2000). PubMed PMID: 10749702
General / background references: Human heat shock factors 1 and 2 are differentially activated and can synergistically induce hsp70 gene transcription. Mol Cell Biol 14:2087-99 (1994). PubMed PMID: 8114740
Jurivich DA et al. Arachidonate is a potent modulator of human heat shock gene transcription. Proc Natl Acad Sci U S A 91:2280-4 (1994). PubMed PMID: 8134388
Rabindran SK et al. Regulation of heat shock factor trimer formation: role of a conserved leucine zipper. Science 259:230-4 (1993). PubMed PMID: 8421783
Sorger PK Heat shock factor and the heat shock response. Cell 65:363-6 (1991). PubMed PMID: 2018972
Rabindran SK et al. Molecular cloning and expression of a human heat shock factor, HSF1. Proc Natl Acad Sci U S A 88:6906-10 (1991). PubMed PMID: 1871105

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Heat shock factor 1 / HSF1 antibody

Western blot analysis of extracts from K562 cells, using HSF1 (Ab-142) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Heat shock factor 1 / HSF1 antibody

Western blot analysis of extracts from Jurkat cells, using HSF1 (Ab-121) antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Heat shock factor 1 / HSF1 (Sumoylation Site) antibody

HSF1 Antibody (Cat. #TA324718) western blot analysis in Jurkat cell line and mouse heart tissue lysates (35ug/lane).This demonstrates the HSF1 antibody detected the HSF1 protein (arrow). Rabbit Ig Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Heat shock factor 1 / HSF1 antibody

Western blot analysis of HSF1 expression in TNF-a treated MCF7 whole cell lysates,The lane on the left is treated with the antigen-specific peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Heat shock factor 1 / HSF1 antibody

HSF1 granules present in heat-shocked mitotic cells (green) detected by IF in mitotic heat shocked HeLa cells.  Courtesy of Morimoto Lab, Northwestern University, USA. Rat IgG1 10H8 Purified Bov, GP, Hst, Hu, Mky, Ms, Rb, Rt E, EMSA, ICC/IF, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Heat shock factor 1 / HSF1 antibody

Western blot analysis on Jurkat cell lysate using HSF1 Antibody Rabbit IgG Purified Hu, Ms WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Heat shock factor 1 / HSF1 antibody

Heat shock factor 1 / HSF1 Rat IgG1 4B4 Purified Hu, Ms, Rt ICC/IF, IP, WB
0.1 mg / €380.00
  Acris Antibodies GmbH

Heat shock factor 1 / HSF1 antibody

Immunohistochemistry: AM03138PU-N HSF1 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Prostate at 15 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Mouse IgG1 J7F9 Purified Hu E, P, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

Heat shock factor 1 / HSF1 antibody

Immunohistochemistry: AM03138PU-N HSF1 antibody staining of Formalin-Fixed, Paraffin-Embedded Human Prostate at 15 µg/ml followed by biotinylated anti-mouse IgG secondary antibody, alkaline phosphatase-streptavidin and chromogen. Mouse IgG1 J7F9 Purified Hu E, P, WB
50 µl / €220.00
  Acris Antibodies GmbH

Heat shock factor 1 / HSF1 antibody

Heat shock factor 1 / HSF1 Rat IgG1 10H8 Purified Bov, GP, Hst, Hu, Mky, Ms, Rb, Rt E, EMSA, ICC/IF, IP, WB
25 µg / €190.00
  Acris Antibodies GmbH

Heat shock factor 1 / HSF1 pSer303 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSF1 antibody pSer303 Cat.-No. AP02511PU (Left) and the same antibody preincubated with blockig peptide (Right). Rabbit Aff - Purified Hu P, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

Heat shock factor 1 / HSF1 pSer303 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSF1 antibody pSer303 Cat.-No. AP02511PU (Left) and the same antibody preincubated with blockig peptide (Right). Rabbit Aff - Purified Hu P, WB
50 µl / €240.00
  Acris Antibodies GmbH

Heat shock factor 1 / HSF1 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using HSF1 antibody. Rabbit Aff - Purified Hu P, WB
0.1 ml / €270.00
  Acris Antibodies GmbH

Heat shock factor 1 / HSF1 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using HSF1 antibody. Rabbit Aff - Purified Hu P, WB
50 µl / €230.00
  Acris Antibodies GmbH

Heat shock factor 1 / HSF1 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSF1 antibody. Rabbit Aff - Purified Hu P, WB
0.1 ml / €270.00
  Acris Antibodies GmbH

Heat shock factor 1 / HSF1 antibody

Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HSF1 antibody. Rabbit Aff - Purified Hu P, WB
50 µl / €230.00
  Acris Antibodies GmbH

Heat shock factor 1 / HSF1 pSer307 antibody

Figure 2. Immunofluorescence staining of methanol-fixed HeLa cells using HSF1 (pSer307) antibody AP08015PU. Rabbit Aff - Purified Hu ICC/IF, WB
0.1 ml / €320.00
  Acris Antibodies GmbH

Heat shock factor 1 / HSF1 pSer307 antibody

Figure 2. Immunofluorescence staining of methanol-fixed HeLa cells using HSF1 (pSer307) antibody AP08015PU. Rabbit Aff - Purified Hu ICC/IF, WB
50 µl / €240.00
  Acris Antibodies GmbH

Heat shock factor 1 / HSF1 antibody

Immunohistochemistry (IHC) analyzes of HSF1 antibody (Cat.-No.: AP20676PU-N) in paraffin-embedded human breast carcinoma tissue. Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Heat shock factor 1 / HSF1 pSer303 antibody

Immunohistochemistry (IHC) analyzes of p-HSF1 antibody (Cat.-No.: AP20867PU-N) in paraffin-embedded human brain tissue. Rabbit Aff - Purified Hu, Ms ICC/IF, P, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

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