Heat shock factor 2 / HSF2 antibody

Principal name

Heat shock factor 2 / HSF2 antibody

Alternative names for Heat shock factor 2 / HSF2 antibody

HSF-2, HSTF2, Heat shock transcription factor 2

SwissProt ID

P38530 (Chick), P38533 (Mouse), Q03933 (Human)

Gene ID

3298 (HSF2), 15500 (Hsf2)

Available reactivities

Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey), Bov (Bovine), Can (Canine), GP (Guinea Pig), Hst (Hamster), Por (Porcine), Rb (Rabbit), Sh (Sheep), Av (Birds), var.

Available hosts

Mouse, Rabbit, Rat

Available applications

Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Paraffin Sections (P), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Frozen Sections (C), Electrophoretic Mobility Shift Assay (EMSA)

Background of Heat shock factor 2 / HSF2 antibody

HSF2, or heat shock factor 2, belongs to a family of Heat Shock transcription factors that activate the transcription of genes encoding products required for protein folding, processing, targeting, degradation, and function (2). The up-regulation of HSP (heat shock proteins) expression by stressors is achieved at the level of transcription through a heat shock element (HSE) and a transcription factor (HSF) (3, 4, 5).
Most HSFs have highly conserved amino acid sequences. On all HSFs there is a DNA binding domain at the Nterminus. Hydrophobic repeats located adjacent to this binding domain are essential for the formation of active trimers. Towards the C-terminal region another short hydrophobic repeat exists, and is thought to be necessary for suppression of trimerization (6).
There are two main heat shock factors, 1 and 2. Mouse HSF1 exists as two isoforms, however in higher eukaryotes HSF1 is found in a diffuse cytoplasmic and nuclear distribution in un-stressed cells. Once exposed to a multitude of stressors, it localizes to discrete nuclear granules within seconds. As it recovers from stress, HSF1 dissipates from these granules to a diffuse nuceloplasmic distribution. HSF2 on the other hand is similar to mouse HSF1, as it exists as two isoforms, the alpha form being more transciptionally active than the smaller beta form (7, 8). Various experiments have suggested that HFS2 may have roles in differentiation and development (9, 10, 11).

General readings

Zhang,J., (2008) J. Biol. Chem. 283 (12), 7464-7469

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Heat shock factor 2 / HSF2 antibody

Immunohistochemical staining of paraffin-embedded Human pancreas tissue within the normal limits using anti-HSF2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1mM EDTA in 10mM Tris buffer (pH8.5) at 120 oC for 3 min, TA807407)(1:150) Mouse IgG1 OTI3E1 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Heat shock factor 2 / HSF2 antibody

Immunohistochemical staining of paraffin-embedded Human adult brain tissue using anti-HSF2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807435)(1:150) Mouse IgG1 OTI2E11 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Heat shock factor 2 / HSF2 antibody

Western blot analysis of extracts from LOVO cells and A549 cells, using HSF2 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Heat shock factor 2 / HSF2 antibody

Predicted band size: 60 kDa . Positive control: Hela cell lysate. Recommended dilution: 1/500-2000. (Gel: 10%SDS-PAGE Lysate: 40 ug Primary antibody: 1/550 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 2 minutes) Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Heat shock factor 2 / HSF2 antibody

Gel: 12%SDS-PAGE, Lysate: 40 µg, Lane: 231 cells, Primary antibody: HSF2 Antibody at dilution 1/400, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 5 minutes Rabbit IgG Purified Hu, Ms, Rt E, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Heat shock factor 2 / HSF2 antibody

HSF2 Antibody (Center) (Cat. #TA324656) western blot analysis in 293T,A549,Hela cell line lysates (35ug/lane).This demonstrates the HSF2 antibody detected the HSF2 protein (arrow). Rabbit IgG Purified Hu WB
0.4 ml / €325.00
  OriGene Technologies, Inc.

Heat shock factor 2 / HSF2 antibody

Western blot analysis (K562 cells) transiently transfected with control,  HSF1 or HSF2 shRNA constructs using HSF1, HSF2 (3E2) antibodies. Courtesy of Lea Sistonen, Abo Akademi University, Finland. Rat IgG1 3E2 Purified var. C, E, EMSA, ICC/IF, IP, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Heat shock factor 2 / HSF2 antibody

WB Suggested Anti-HSF2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:12500; Positive Control: 721_B cell lysateHSF2 is supported by BioGPS gene expression data to be expressed in 721_B Rabbit IgG Purified Hu WB
50 µg / €325.00
  OriGene Technologies, Inc.

Heat shock factor 2 / HSF2 antibody

WB Suggested Anti-Hsf2 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:312500; Positive Control: Mouse Uterus Rabbit IgG Purified Hu, Ms, Rb, Rt WB
50 µg / €325.00
  OriGene Technologies, Inc.

Heat shock factor 2 / HSF2 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSF2 (RC210751, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSF2.(1:2000) Mouse IgG1 OTI2E2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Heat shock factor 2 / HSF2 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSF2 (RC210751, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSF2.(1:2000) Mouse IgG1 OTI4G9 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Heat shock factor 2 / HSF2 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSF2 (RC210751, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSF2.(1:2000) Mouse IgG1 OTI2F11 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Heat shock factor 2 / HSF2 antibody

Immunohistochemical staining of paraffin-embedded Human bladder tissue within the normal limits using anti-HSF2 mouse monoclonal antibody. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min, TA807434) Mouse IgG1 OTI2A7 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Heat shock factor 2 / HSF2 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSF2 (RC210751, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSF2. Mouse IgG2a OTI4B2 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Heat shock factor 2 / HSF2 antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HSF2 (RC210751, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HSF2. Mouse IgG1 OTI5C1 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Heat shock factor 2 / HSF2 antibody

Western blot analysis (K562 cells) transiently transfected with control, HSF1 or HSF2 shRNA constructs using HSF1, HSF2 (3E2) antibodies. Courtesy of Lea Sistonen, Abo Akademi University, Finland. Rat IgG1 3E2 Purified var. C, E, EMSA, ICC/IF, IP, WB
25 µg / €190.00
  Acris Antibodies GmbH

Heat shock factor 2 / HSF2 antibody

Western blot analysis of HSF2 antibody (Cat.-No.: AP06712PU-N)  in extracts from LOVO cells Rabbit Aff - Purified Hu, Ms, Rt E, WB
0.1 mg / €350.00
  Acris Antibodies GmbH

Heat shock factor 2 / HSF2 antibody

Heat shock factor 2 / HSF2 Mouse Serum Hu E, WB
50 µl / €290.00
  Abnova Taiwan Corp.

Heat shock factor 2 / HSF2 antibody

Heat shock factor 2 / HSF2 Mouse Hu WB
0.5 mg / €1,400.00
  Abnova Taiwan Corp.

Heat shock factor 2 / HSF2 antibody

Detection limit for recombinant GST tagged HSF2 is approximately 0.3ng/ml as a capture antibody. Mouse IgG1 1F11-A3 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

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