Heat shock protein 70 / HSP70 antibody

Principal name

Heat shock protein 70 / HSP70 antibody

Alternative names for Heat shock protein 70 / HSP70 antibody

HSP70.1, HSP70-1/HSP70-2, HSPA1A, HSPA1B, HSPA1, Heat shock 70 kDa protein 1A/1B

SwissProt ID

P02825 (Drome), P08107 (Human), P10591 (Yeast), P22953 (Arath), P25840 (Chlre), Q07439 (Rat), Q28222 (Chlae), Q7YQC6 (Canfa), Q9I8F9 (Oryla)

Gene ID

3303 (HSPA1A), 831020 (HSC70-1), 24472 (Hspa1a), 3304 (HSPA1B), 294254 (Hspa1b)

Available reactivities

Bov (Bovine), Can (Canine), Chk (Chicken), GP (Guinea Pig), Hst (Hamster), Hu (Human), Mky (Monkey), Ms (Mouse), Rt (Rat), Sh (Sheep), Por (Porcine), Fe (Feline), Gt (Goat), Ce (C. elegans), Ze (Zebrafish), African clawed frog, Carp, Dros (Drosophila), Rb (Rabbit), Arabidopsis, Fi (Fish), Salmon, Trout, Amph (Amphibian), Av (Birds), S. cerevisiae (Saccharomyces cerevisiae)

Available hosts

Mouse, Rabbit, Chicken

Available applications

Frozen Sections (C), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Flow Cytometry (F), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoelectron Microscopy (EM), Luminex (LMNX)

Background of Heat shock protein 70 / HSP70 antibody

Heat-shock proteins, or stress proteins, are expressed in response to heat shock and a variety of other stress stimuli including oxidative free radicals and toxic metal ions. Sargent et al. (1989) identified a duplicated HSP70 locus in the class III region of the major histocompatibility complex on 6p21.3. A duplicated locus encoding the major heat shock-induced protein HSP70 is located in the major histocompatibility complex (MHC) class III region 92 kilobases (kb) telomeric to the C2 gene. The 70-kd mammalian heat shock proteins are structurally and functionally related to the uncoating protein that releases clathrin triskelia from coated vesicles.The 70 kilodalton heat shock proteins (Hsp70s) are a family of ubiquitously expressed heat shock proteins. The Hsp70s are an important part of the cell's machinery for protein folding, and help to protect cells from stress. All of the Hsp70 proteins have three major functional domains: An N-terminal ATPase domain binds ATP (Adenosine triphosphate) and hydrolyzes it to ADP (Adenosine diphosphate); A substrate binding domain contains a groove with an affinity for neutral, hydrophobic amino acid residues; A C-terminal domain rich in alpha helical structure acts as a 'lid' for the substrate binding domain. By binding tightly to partially-synthesized peptide sequences (incomplete proteins),Hsp70 prevents them from aggregating and being rendered nonfunctional. And it also can act to protect cells from thermal or oxidative stress. Finally, Hsp70 seems to be able to participate in disposal of damaged or defective proteins. Interaction with CHIP (Carboxyl-terminus of Hsp70 Interacting Protein)–an E3 ubiquitin ligase–allows Hsp70 to pass proteins to the cell's ubiquitination and proteolysis pathways.

General readings

. Welch W.J. and Suhan J.P. (1986) J.Cell Biol. 103: 2035-2050.
2. Boorstein W. R., Ziegelhoffer T. & Craig E. A. (1993), J. Mol. Evol.38 (1): 1-17.
3. Rothman J. (1989) Cell 59: 591 -601.
4. DeLuca-Flaherty et al. (1990), Cell 62: 875-887.
5. Bork P., Sander C. & Valencia A. (1992) Proc. Nut1 Acad. Sci. USA 89: 7290-7294.
6. Fink A.L. (1999) Physiol. Rev. 79: 425-449.
7. Polanonka-Grabowska R. et. al. (1997) Blood 90: 1516-1526.
8. Schnell D.J. et. al. (1994) Science 266: 1007-1012.
9. Kabakov A.E., et. al. (2002) Am. J.Physiol. 283(2): C521-C534.
10. Ricart J. et. al. (1997) Biochem. J. 324: 635-643.
11. Hang H. and Fox M.H. (1995) Cytometry 19(2): 119-125.

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Heat shock protein 70 / HSP70 antibody

  Mouse Monoclonal anti-HSPA1A Antibody  
FACS analysis. Anti-Hsp70-FITC staining on heat shock treated CD3+CD8+T cells. Mouse IgG1 C92F3A-5 FITC Bov, Can, Carp, Chk, Dros, GP, Hst, Hu, Mky, Ms, Por, Rb, Rt, Sh F
0.2 mg / €325.00
  OriGene Technologies, Inc.

Heat shock protein 70 / HSP70 antibody

  Mouse monoclonal Hsp70 Antibody  
Western blot analysis of Hsp70 in cell lysates from 12 human cancer cell lines at 1:1000 dilution of TA326356. Mouse IgG1 C92F3A-5 Aff - Purified Bov, Can, Carp, Ce, Chk, Dros, GP, Hst, Hu, Mky, Ms, Por, Rb, Rt, Sh C, E, EM, F, ICC/IF, IP, WB
0.2 mg / €325.00
  OriGene Technologies, Inc.

Heat shock protein 70 / HSP70 antibody

  Rabbit polyclonal Hsp70 Antibody  
Western blot analysis of Hsp70 in Pam212 cells using a 1:1000 dilution of the antibody Rabbit Serum Beluga, Bov, Can, Coral, Fi, GP, Hst, Hu, Mky, Ms, Por, Rt, Sh, Trout C, E, ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Heat shock protein 70 / HSP70 antibody

  Mouse monoclonal anti-HSPA1A(HSP70) antibody, clone 3C6, Loadi, clone OTI3C6 (formerly 3C6)  
Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-HSPA1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500772, Dilution 1:50) Mouse IgG1 OTI3C6 Purified Can, Hu, Mky F, ICC/IF, P, WB
0.1 ml / €248.00
  OriGene Technologies, Inc.

TrueMAB

Heat shock protein 70 / HSP70 antibody

  Rabbit polyclonal anti-HSPA1A(HSP70) antibody, Loading control  
Sample (whole cell lysate). A: 293T 20ug. B: 293T 10ug. C: 293T 5ug. D: 293T 1ug. 7.5% SDS PAGE. TA308905 diluted at 1:10000. Rabbit IgG Aff - Purified Hu, Ms ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Heat shock protein 70 / HSP70 antibody

  anti-HSPA1A (Hsp70) mouse monoclonal antibody, clone OTI2C12 (formerly 2C12)  
Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-HSPA1Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500731, Dilution 1:50) Mouse IgG1 OTI2C12 Purified Can, Hu, Mky ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Heat shock protein 70 / HSP70 antibody

  anti-HSPA1A (Hsp70) mouse monoclonal antibody, clone OTI5F3 (formerly 5F3)  
Immunofluorescent staining of HepG2 cells using anti-HSPA1A mouse monoclonal antibody (TA500770). Mouse IgG1 OTI5F3 Purified Can, Hu, Mky F, ICC/IF, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Heat shock protein 70 / HSP70 antibody

  anti-HSPA1A (Hsp70) mouse monoclonal antibody, clone OTI3G9 (formerly 3G9)  
Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-HSPA1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500771, Dilution 1:50) Mouse IgG1 OTI3G9 Purified Can, Hu, Mky ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Heat shock protein 70 / HSP70 antibody

  anti-HSPA1A (Hsp70) mouse monoclonal antibody, clone OTI1B2 (formerly 1B2)  
Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-HSPA1Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500827, Dilution 1:50) Mouse IgG1 OTI1B2 Purified Can, Hu, Mky P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Heat shock protein 70 / HSP70 antibody

  anti-HSPA1A (Hsp70) mouse monoclonal antibody, clone OTI1H5 (formerly 1H5)  
Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-HSPA1Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500828, Dilution 1:50) Mouse IgG2b OTI1H5 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Heat shock protein 70 / HSP70 antibody

Paraffin embedded sections of human eccrine gland were incubated with anti-human Hsp70 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB) Mouse IgG1 4E7 Purified Hu E, P, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

Heat shock protein 70 / HSP70 antibody

Paraffin embedded sections of human eccrine gland were incubated with anti-human Hsp70 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB) Mouse IgG1 4E7 Purified Hu E, P, WB
50 µl / €230.00
  Acris Antibodies GmbH

Heat shock protein 70 / HSP70 antibody

  Rabbit Polyclonal Antibody against HSPA1A (Y41)  
Western blot analysis of HSPA1A (arrow) using rabbit polyclonal HSPA1A Antibody (S41) (Cat.#TA302005). Rabbit Ig Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Heat shock protein 70 / HSP70 antibody

  Rabbit Polyclonal Antibody against HSPA1A (Y525)  
Western blot analysis of HSPA1A (arrow) using rabbit polyclonal HSPA1A Antibody (Y525) (Cat.#TA302006). Rabbit Ig Aff - Purified Hu WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Heat shock protein 70 / HSP70 antibody

  Chicken Polyclonal anti-HSPA1A Antibody  
Western blot analysis of Hsp70 in HeLa cell lysates using a 1:1000 dilution of TA309382. Chicken IgY Purified Hu, Ms, Rt E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Heat shock protein 70 / HSP70 antibody

  Anti-HSPA1A Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm, Nucleus. Positive control: Human lung cancer tissue. Recommended dilution: 1/50-100  The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using HSPA1A antibody at dilution 1/60, on the right is treated with the fusion protein. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Heat shock protein 70 / HSP70 antibody

  Anti-HSPA1A Rabbit Polyclonal Antibody  
Predicted cell location: Cytoplasm. Positive control: Human colon cancer tissue. Recommended dilution: 1/50-100  The image on the left is immunohistochemistry of paraffin-embedded Human colon cancer tissue using HSPA1A antibody at dilution 1/40, on the right is treated with the fusion protein. (Original magnification: x200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

Heat shock protein 70 / HSP70 antibody

  Mouse monoclonal Hsp70 Antibody  
Western blot analysis of Hsp70 in rat tissue lysates, using a dilution of the antibody Mouse IgG1 5A5 Aff - Purified Amph, Av, Dros, Fi, Hu, Ms, Rb, Rt, S. cerevisiae ICC/IF, IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Heat shock protein 70 / HSP70 antibody

  Mouse monoclonal Hsp70 Antibody  
Western blot analysis of Hsp70 in rat tissue mix using a 1:5000 dilution of the antibody Mouse IgM 2A4 Purified Amph, Chk, Dros, Fi, Hu, Ms, Rb, Rt, S. cerevisiae C, ICC/IF, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Heat shock protein 70 / HSP70 antibody

  Mouse monoclonal Hsp70 Antibody  
10ug cell free extract from Artemia probed with anti-Hsp70 Mouse IgG1 3A3 Aff - Purified Amph, Chk, Dros, Fi, Hu, Ms, Rb, Rt, S. cerevisiae ICC/IF, IP, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

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