Heat shock protein 70 / HSP70 antibody

Principal name

Heat shock protein 70 / HSP70 antibody

Alternative names for Heat shock protein 70 / HSP70 antibody

HSP70.1, HSP70-1/HSP70-2, HSPA1A, HSPA1B, HSPA1, Heat shock 70 kDa protein 1A/1B

SwissProt ID

P02825 (Drome), P08107 (Human), P10591 (Yeast), P22953 (Arath), P25840 (Chlre), Q07439 (Rat), Q28222 (Chlae), Q7YQC6 (Canfa), Q9I8F9 (Oryla)

Gene ID

3303 (HSPA1A), 831020 (HSC70-1), 24472 (Hspa1a)

Available reactivities

Bov (Bovine), Can (Canine), Chk (Chicken), GP (Guinea Pig), Hst (Hamster), Hu (Human), Mky (Monkey), Ms (Mouse), Rt (Rat), Sh (Sheep), Por (Porcine), Fe (Feline), Gt (Goat), Ce (C. elegans), Ze (Zebrafish), African clawed frog, Rb (Rabbit), Dros (Drosophila), Fi (Fish), Ye (Yeast), Pl (Plant), Amph (Amphibian), Av (Birds), Carp, Trout, Arabidopsis, Xen (Xenopus), Salmon, S. cerevisiae (Saccharomyces cerevisiae)

Available hosts

Mouse, Rabbit, Chicken

Available applications

Frozen Sections (C), Immunoprecipitation (IP), Western blot / Immunoblot (WB), Flow Cytometry (F), Paraffin Sections (P), Enzyme Immunoassay (E), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoelectron Microscopy (EM), Electrophoretic Mobility Shift Assay (EMSA), Luminex (LMNX)

Background of Heat shock protein 70 / HSP70 antibody

Heat-shock proteins, or stress proteins, are expressed in response to heat shock and a variety of other stress stimuli including oxidative free radicals and toxic metal ions. Sargent et al. (1989) identified a duplicated HSP70 locus in the class III region of the major histocompatibility complex on 6p21.3. A duplicated locus encoding the major heat shock-induced protein HSP70 is located in the major histocompatibility complex (MHC) class III region 92 kilobases (kb) telomeric to the C2 gene. The 70-kd mammalian heat shock proteins are structurally and functionally related to the uncoating protein that releases clathrin triskelia from coated vesicles.The 70 kilodalton heat shock proteins (Hsp70s) are a family of ubiquitously expressed heat shock proteins. The Hsp70s are an important part of the cell's machinery for protein folding, and help to protect cells from stress. All of the Hsp70 proteins have three major functional domains: An N-terminal ATPase domain binds ATP (Adenosine triphosphate) and hydrolyzes it to ADP (Adenosine diphosphate); A substrate binding domain contains a groove with an affinity for neutral, hydrophobic amino acid residues; A C-terminal domain rich in alpha helical structure acts as a 'lid' for the substrate binding domain. By binding tightly to partially-synthesized peptide sequences (incomplete proteins),Hsp70 prevents them from aggregating and being rendered nonfunctional. And it also can act to protect cells from thermal or oxidative stress. Finally, Hsp70 seems to be able to participate in disposal of damaged or defective proteins. Interaction with CHIP (Carboxyl-terminus of Hsp70 Interacting Protein)–an E3 ubiquitin ligase–allows Hsp70 to pass proteins to the cell's ubiquitination and proteolysis pathways.

General readings

This product has been used in:Wimmer B et al. The glyoxysomal and plastid molecular chaperones (70-kDa heat shock protein) of watermelon cotyledons are encoded by a single gene. Proc Natl Acad Sci U S A 94:13624-9 (1997). PubMed PMID: 9391076
Velez-Granell CS et al. Molecular chaperones in pancreatic tissue: the presence of cpn10, cpn60 and hsp70 in distinct compartments along the secretory pathway of the acinar cells. J Cell Sci 107 (Pt 3):539-49 (1994). PubMed PMID: 7911805
Marchesi VT & Ngo N In vitro assembly of multiprotein complexes containing alpha, beta, and gamma tubulin, heat shock protein HSP70, and elongation factor 1 alpha. Proc Natl Acad Sci U S A 90:3028-32 (1993). PubMed PMID: 8464918
General / background references:Hamos JE et al. Expression of heat shock proteins in Alzheimer's disease. Neurology 41:345-50 (1991). PubMed PMID: 2005999
Kaufmann SH Heat shock proteins and the immune response. Immunol Today 11:129-36 (1990). PubMed PMID: 2187470
Omar R et al. Immunocytochemical detection of the 70-kd heat shock protein in alcoholic liver disease. Arch Pathol Lab Med 114:589-92 (1990). PubMed PMID: 1693268
Mizzen LA et al. Identification, characterization, and purification of two mammalian stress proteins present in mitochondria, grp 75, a member of the hsp 70 family and hsp 58, a homolog of the bacterial groEL protein. J Biol Chem 264:20664-75 (1989). PubMed PMID: 2573603
Reading DS et al. Characterization of the yeast HSP60 gene coding for a mitochondrial assembly factor. Nature 337:655-9 (1989). PubMed PMID: 2563898
Reading DS et al. Characterization of the yeast HSP60 gene coding for a mitochondrial assembly factor. Nature 337:655-9 (1989). PubMed PMID: 2563898
Lindquist S & Craig EA The heat-shock proteins. Annu Rev Genet 22:631-77 (1988). PubMed PMID: 2853609
Pelham HR Speculations on the functions of the major heat shock and glucose-regulated proteins. Cell 46:959-61 (1986). PubMed PMID: 2944601
Velazquez JM & Lindquist S hsp70: nuclear concentration during environmental stress and cytoplasmic storage during recovery. Cell 36:655-62 (1984). PubMed PMID: 6421488

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Heat shock protein 70 / HSP70 antibody

FACS analysis. Anti-Hsp70-FITC staining on heat shock treated CD3+CD8+T cells. Mouse IgG1 C92F3A-5 FITC Bov, Can, Carp, Chk, Dros, GP, Hst, Hu, Mky, Ms, Por, Rb, Rt, Sh F
0.2 mg / €325.00
  OriGene Technologies, Inc.

Heat shock protein 70 / HSP70 antibody

Western blot analysis of Hsp70 in cell lysates from 12 human cancer cell lines at 1:1000 dilution of TA326356. Mouse IgG1 C92F3A-5 Aff - Purified Bov, Can, Carp, Ce, Chk, Dros, GP, Hst, Hu, Mky, Ms, Por, Rb, Rt, Sh C, E, EM, F, ICC/IF, IP, WB
0.2 mg / €325.00
  OriGene Technologies, Inc.

Heat shock protein 70 / HSP70 antibody

Western blot analysis of Hsp70 in Pam212 cells using a 1:1000 dilution of the antibody Rabbit Serum Beluga, Bov, Can, Coral, Fi, GP, Hst, Hu, Mky, Ms, Por, Rt, Sh, Trout C, E, ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Heat shock protein 70 / HSP70 antibody

Immunohistochemical staining of paraffin-embedded Human liver tissue within the normal limits using anti-HSPA1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500772, Dilution 1:50) Mouse IgG1 OTI3C6 Purified Can, Hu, Mky F, ICC/IF, P, WB
0.1 ml / €248.00
  OriGene Technologies, Inc.

TrueMAB

Heat shock protein 70 / HSP70 antibody

FACS analysis. Anti-Hsp70-FITC staining on heat shock treated CD3+CD8+T cells. Mouse IgG1 C92F3A-5 FITC Bov, Can, Carp, Chk, Dros, GP, Hst, Hu, Mky, Ms, Por, Rb, Rt, Sh F
50 µg / €240.00
  Acris Antibodies GmbH

Heat shock protein 70 / HSP70 antibody

Western blot analysis of Hsp70 in cell lysates from 12 human cancer cell lines at 1:1000 dilution of TA326356. Mouse IgG1 C92F3A-5 Aff - Purified Bov, Can, Carp, Ce, Chk, Dros, GP, Hst, Hu, Mky, Ms, Por, Rb, Rt, Sh C, E, EM, F, ICC/IF, IP, WB
50 µg / €230.00
  Acris Antibodies GmbH

Heat shock protein 70 / HSP70 antibody

Sample (whole cell lysate). A: 293T 20ug. B: 293T 10ug. C: 293T 5ug. D: 293T 1ug. 7.5% SDS PAGE. TA308905 diluted at 1:10000. Rabbit IgG Aff - Purified Hu, Ms ICC/IF, IP, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Heat shock protein 70 / HSP70 antibody

Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-HSPA1Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500731, Dilution 1:50) Mouse IgG1 OTI2C12 Purified Can, Hu, Mky ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Heat shock protein 70 / HSP70 antibody

Immunofluorescent staining of HepG2 cells using anti-HSPA1A mouse monoclonal antibody (TA500770). Mouse IgG1 OTI5F3 Purified Can, Hu, Mky F, ICC/IF, WB
0.1 ml / €379.00
  OriGene Technologies, Inc.

TrueMAB

Heat shock protein 70 / HSP70 antibody

Immunohistochemical staining of paraffin-embedded pancreas tissue within the normal limits using anti-HSPA1A mouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500771, Dilution 1:50) Mouse IgG1 OTI3G9 Purified Can, Hu, Mky ICC/IF, P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Heat shock protein 70 / HSP70 antibody

Immunohistochemical staining of paraffin-embedded liver tissue within the normal limits using anti-HSPA1Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500827, Dilution 1:50) Mouse IgG1 OTI1B2 Purified Can, Hu, Mky P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Heat shock protein 70 / HSP70 antibody

Immunohistochemical staining of paraffin-embedded Carcinoma of liver tissue using anti-HSPA1Amouse monoclonal antibody. (Heat-induced epitope retrieval by 10mM citric buffer, pH6.0, 100C for 10min, TA500828, Dilution 1:50) Mouse IgG2b OTI1H5 Purified Hu P, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Heat shock protein 70 / HSP70 antibody

Paraffin embedded sections of human eccrine gland were incubated with anti-human Hsp70 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB) Mouse IgG1 4E7 Purified Hu E, P, WB
0.1 ml / €310.00
  Acris Antibodies GmbH

Heat shock protein 70 / HSP70 antibody

Paraffin embedded sections of human eccrine gland were incubated with anti-human Hsp70 (1:100) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium citrate buffer and detected using Diaminobenzidine (DAB) Mouse IgG1 4E7 Purified Hu E, P, WB
50 µl / €230.00
  Acris Antibodies GmbH

Heat shock protein 70 / HSP70 antibody

Immunoperoxidase of monoclonal antibody to HSPA1B on formalin-fixed paraffin-embedded human testis. [antibody concentration 6 ug/ml] Mouse IgG2a 3B7 Purified Hu, Ms, Rt E, ICC/IF, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Heat shock protein 70 / HSP70 antibody

Immunoperoxidase of monoclonal antibody to HSPA1B on formalin-fixed paraffin-embedded human esophagus. [antibody concentration 3 ug/ml] Mouse IgG2a 2D11 Purified Hu, Ms, Rt E, P, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

Heat shock protein 70 / HSP70 antibody

Immunohistochemistry: Hsp70 Antibody(C92) [NB110-61582]-Mouse Colon Cancer Mouse IgG C92F3A-5 Purified Bov, Can, Ce, Chk, Dros, Fi, GP, Hu, Mky, Ms, Por, Rb, Rt, Sh E, F, ICC/IF, P, WB
0.2 mg / €480.00
  Novus Biologicals Inc.

Heat shock protein 70 / HSP70 (574-591) antibody

AP15595PU HSP70 antibody staining of Human Breast Carcinoma. Rabbit Purified Hu, Ms, Rt P, WB
0.5 ml / €360.00
  Acris Antibodies GmbH

Heat shock protein 70 / HSP70 (574-591) antibody

AP15595PU HSP70 antibody staining of Human Breast Carcinoma. Rabbit Purified Hu, Ms, Rt P, WB
1 ml / €520.00
  Acris Antibodies GmbH

Heat shock protein 70 / HSP70 (574-591) antibody

AP15595PU HSP70 antibody staining of Human Breast Carcinoma. Rabbit Purified Hu, Ms, Rt P, WB
0.1 ml / €190.00
  Acris Antibodies GmbH

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