Helicard antibody

Principal name

Helicard antibody

Alternative names for Helicard antibody

IFIH1, MDA5, RH116, Interferon-induced helicase C domain-containing protein 1, Helicase with 2 CARD domains, Melanoma differentiation-associated protein 5, RNA helicase-DEAD box protein 116, Murabutide down-regulated protein

SwissProt ID

Q8R5F7 (Mouse), Q9BYX4 (Human)

Gene ID

64135 (IFIH1), 71586 (Ifih1)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Por (Porcine), Rt (Rat), Eq (Equine)

Available hosts

Goat, Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Frozen Sections (C), Paraffin Sections (P), Western blot / Immunoblot (WB), ELISA (detection) (E(detection)), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of Helicard antibody

Antiviral inte immunity depends on the combition of parallel pathways triggered by virus detecting proteins in the Toll-like receptor (TLR) family and R helicases, such as Rig-I (retinoic acid-inducible gene I) and MDA-5 (melanoma differentiation-associated antigen 5), which promote the transcription of type I interferons (IFN) and antiviral enzymes. TLRs and helicase proteins contain sites that recognize the molecular patterns of different virus types, including D, single-stranded R (ssR), double-stranded R (dsR), and glycoproteins. These antiviral proteins are found in different cell compartments; TLRs (i.e. TLR3, TLR7, TLR8, and TLR9) are expressed on endosomal membranes and helicases are localized to the cytoplasm. Rig-I expression is induced by retinoic acid, LPS, IFN, and viral infection. Both Rig-I and MDA-5 share a DExD/H-box helicase domain that detects viral dsR and two amino-termil caspase recruitment domains (CARD) that are required for triggering downstream sigling . Rig-I binds both dsR and viral ssR that contains a 5-triphosphate end not seen in host R. Though structurally related, Rig-I and MDA-5 detect a distinct set of viruses. The CARD domain of the helicases, which is sufficient to generate sigling and IFN production, is recruited to the CARD domain of the MAVS/VISA/Cardif/IPS-1 mitochondrial protein, which triggers activation of NF-¦ÊB, TBK1/IKK¦Å, and IRF-3/IRF-7.MDA-5, also med Ifih1 (interferon induced with helicase C domain 1), RH116 (R helicase-DEAD box protein 116), or Helicard is found to be induced by interferon. During apoptosis, MDA-5 is cleaved by caspases, separating the helicase and CARD domains. MDA-5 is uniquely activated by picorvirusand measles virus .

General readings

1. Sato S, Hoshino K, Satoh T, Fujita T, Kawakami Y, Fujita T, Kuwana M. RNA helicase encoded by melanoma differentiation-associated gene 5 is a major autoantigen in patients with clinically amyopathic dermatomyositis: Association with rapidly progressive interstitial lung disease. Arthritis Rheum. 2009 Jul;60(7):2193-200.
PMID: 19565506
2. Liao S, Bao X, Liu T, Lai S, Li K, Garofalo RP, Casola A.
Role of retinoic acid inducible gene-I in human metapneumovirus-induced cellular signalling. J Gen Virol. 2008 Aug;89(Pt 8):1978-86. PMID: 18632970
3. Bao X, Sinha M, Liu T, Hong C, Luxon BA, Garofalo RP, Casola A. Identification of human metapneumovirus-induced gene networks in airway epithelial cells by microarray analysis. Virology. 2008 Apr 25;374(1):114-27. PMID: 18234263

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Helicard (N-term) antibody

TA302870 (10µg/ml) staining of paraffin embedded Human Pancreas. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. Goat Aff - Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Helicard (C-term) antibody

Western blot analysis of MDA5 in Daudi cell lysate with MDA5 antibody at (A) 1, (B) 2 and (C) 4 ug/ml. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Helicard (Center) antibody

Western blot analysis of MDA5 in Daudi cell lysate with MDA5 antibody at (A) 1, (B) 2 and (C) 4 ug/ml. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Helicard antibody

Western blot analysis of extracts from Jurkat cells, using IFIH1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Helicard antibody

Western blot analysis of extracts of Jurkat cell lines, using IFIH1 antibody. Rabbit IgG Purified Hu
0.1 mg / €325.00
  OriGene Technologies, Inc.

Helicard antibody

WB Suggested Anti-IFIH1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human Muscle Rabbit IgG Purified Bov, Eq, Hu, Ms, Por, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Helicard antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IFIH1 (RC215661, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IFIH1. Mouse IgG1 OTI8C10 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Helicard antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IFIH1 (RC215661, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IFIH1. Mouse IgG2a OTI3E10 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Helicard antibody

HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IFIH1 (RC215661, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IFIH1. Mouse IgG1 OTI11C11 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Helicard antibody

Pancreas: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Helicard antibody

Liver: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Helicard Control Peptide

Helicard
50 µg / €160.00
  Acris Antibodies GmbH

Helicard Control Peptide

Helicard
50 µg / €160.00
  Acris Antibodies GmbH

Helicard antibody

Western Blot analysis of IFIH1 expression in transfected 293T cell line (H00064135-T01) by IFIH1 MaxPab polyclonal antibody.

Lane 1: IFIH1 transfected lysate(24.31 KDa).
Lane 2: Non-transfected lysate. Mouse Purified Hu E(detection), WB
50 µg / €370.00
  Abnova Taiwan Corp.

Helicard antibody

Western Blot analysis of IFIH1 expression in transfected 293T cell line (H00064135-T02) by IFIH1 MaxPab polyclonal antibody.

Lane 1: IFIH1 transfected lysate(25.10 KDa).
Lane 2: Non-transfected lysate. Rabbit Serum Hu E(detection), IP, WB
0.1 ml / €380.00
  Abnova Taiwan Corp.

Helicard antibody

Western Blot analysis of IFIH1 expression in transfected 293T cell line (H00064135-T02) by IFIH1 MaxPab polyclonal antibody.

Lane 1: IFIH1 transfected lysate(25.10 KDa).
Lane 2: Non-transfected lysate. Rabbit Purified Hu E(detection), WB
0.1 mg / €380.00
  Abnova Taiwan Corp.

Helicard antibody

Immunoperoxidase of monoclonal antibody to IFIH1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml] Mouse IgG2a 3F6 Purified Hu E, P
0.1 mg / €370.00
  Abnova Taiwan Corp.

Helicard antibody

IFIH1 polyclonal antibody ( Cat # PAB6401 ) staining ( 10 µg/ml ) of paraffin embedded human pancreas.  Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. Goat Hu E, P
0.1 mg / €400.00
  Abnova Taiwan Corp.

Helicard antibody

Helicard Goat Ms E
0.1 mg / €390.00
  Abnova Taiwan Corp.

Helicard antibody

Immunohistochemistry of IFIH1 in human lymph node tissue with IFIH1 polyclonal antibody ( Cat # PAB13246 ) at 5 µg/ml. Rabbit Hu, Ms P, WB
0.1 mg / €390.00
  Abnova Taiwan Corp.

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