Helicard antibody

Principal name

Helicard antibody

Alternative names for Helicard antibody

IFIH1, MDA5, RH116, Interferon-induced helicase C domain-containing protein 1, Helicase with 2 CARD domains, Melanoma differentiation-associated protein 5, RNA helicase-DEAD box protein 116, Murabutide down-regulated protein

SwissProt ID

Q8R5F7 (Mouse), Q9BYX4 (Human)

Gene ID

71586 (Ifih1), 64135 (IFIH1)

Available reactivities

Hu (Human), Ms (Mouse), Bov (Bovine), Por (Porcine), Rt (Rat), Eq (Equine)

Available hosts

Goat, Mouse, Rabbit

Available applications

Enzyme Immunoassay (E), Paraffin Sections (P), ELISA (detection) (E(detection)), Western blot / Immunoblot (WB), Immunoprecipitation (IP), Immunocytochemistry/Immunofluorescence (ICC/IF)

Background of Helicard antibody

Antiviral inte immunity depends on the combition of parallel pathways triggered by virus detecting proteins in the Toll-like receptor (TLR) family and R helicases, such as Rig-I (retinoic acid-inducible gene I) and MDA-5 (melanoma differentiation-associated antigen 5), which promote the transcription of type I interferons (IFN) and antiviral enzymes. TLRs and helicase proteins contain sites that recognize the molecular patterns of different virus types, including D, single-stranded R (ssR), double-stranded R (dsR), and glycoproteins. These antiviral proteins are found in different cell compartments; TLRs (i.e. TLR3, TLR7, TLR8, and TLR9) are expressed on endosomal membranes and helicases are localized to the cytoplasm. Rig-I expression is induced by retinoic acid, LPS, IFN, and viral infection. Both Rig-I and MDA-5 share a DExD/H-box helicase domain that detects viral dsR and two amino-termil caspase recruitment domains (CARD) that are required for triggering downstream sigling . Rig-I binds both dsR and viral ssR that contains a 5-triphosphate end not seen in host R. Though structurally related, Rig-I and MDA-5 detect a distinct set of viruses. The CARD domain of the helicases, which is sufficient to generate sigling and IFN production, is recruited to the CARD domain of the MAVS/VISA/Cardif/IPS-1 mitochondrial protein, which triggers activation of NF-¦ÊB, TBK1/IKK¦Å, and IRF-3/IRF-7.MDA-5, also med Ifih1 (interferon induced with helicase C domain 1), RH116 (R helicase-DEAD box protein 116), or Helicard is found to be induced by interferon. During apoptosis, MDA-5 is cleaved by caspases, separating the helicase and CARD domains. MDA-5 is uniquely activated by picorvirusand measles virus .

General readings

1. Sato S, Hoshino K, Satoh T, Fujita T, Kawakami Y, Fujita T, Kuwana M. RNA helicase encoded by melanoma differentiation-associated gene 5 is a major autoantigen in patients with clinically amyopathic dermatomyositis: Association with rapidly progressive interstitial lung disease. Arthritis Rheum. 2009 Jul;60(7):2193-200.
PMID: 19565506
2. Liao S, Bao X, Liu T, Lai S, Li K, Garofalo RP, Casola A.
Role of retinoic acid inducible gene-I in human metapneumovirus-induced cellular signalling. J Gen Virol. 2008 Aug;89(Pt 8):1978-86. PMID: 18632970
3. Bao X, Sinha M, Liu T, Hong C, Luxon BA, Garofalo RP, Casola A. Identification of human metapneumovirus-induced gene networks in airway epithelial cells by microarray analysis. Virology. 2008 Apr 25;374(1):114-27. PMID: 18234263

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

Helicard (N-term) antibody

  Goat Polyclonal Antibody against IFIH1 (MDA5)  
TA302870 (10µg/ml) staining of paraffin embedded Human Pancreas. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining. Goat Aff - Purified Hu E, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Helicard (C-term) antibody

  Rabbit Polyclonal MDA5 Antibody  
Western blot analysis of MDA5 in Daudi cell lysate with MDA5 antibody at (A) 1, (B) 2 and (C) 4 ug/ml. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Helicard (Center) antibody

  Rabbit Polyclonal MDA5 Antibody  
Western blot analysis of MDA5 in Daudi cell lysate with MDA5 antibody at (A) 1, (B) 2 and (C) 4 ug/ml. Rabbit IgG Aff - Purified Hu, Ms E, ICC/IF, P, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

Helicard antibody

  Rabbit polyclonal IFIH1 (MDA5) antibody  
Western blot analysis of extracts from Jurkat cells, using IFIH1 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

Helicard antibody

  IFIH1 Rabbit Polyclonal Antibody  
Western blot analysis of extracts of Jurkat cell lines, using IFIH1 antibody. Rabbit IgG Purified Hu
0.1 mg / €325.00
  OriGene Technologies, Inc.

Helicard antibody

  Rabbit Polyclonal Anti-IFIH1 Antibody  
WB Suggested Anti-IFIH1 Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: Human Muscle Rabbit IgG Purified Bov, Eq, Hu, Ms, Por, Rt P, WB
50 µg / €325.00
  OriGene Technologies, Inc.

Helicard antibody

  IFIH1 (MDA5)ᅠmouse monoclonal antibody, clone OTI8C10 (formerly 8C10)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IFIH1 (RC215661, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IFIH1. Mouse IgG1 OTI8C10 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Helicard antibody

  IFIH1 (MDA5)ᅠmouse monoclonal antibody, clone OTI3E10 (formerly 3E10)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IFIH1 (RC215661, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IFIH1. Mouse IgG2a OTI3E10 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Helicard antibody

  IFIH1 (MDA5)ᅠmouse monoclonal antibody, clone OTI11C11 (formerly 11C11)  
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY IFIH1 (RC215661, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-IFIH1. Mouse IgG1 OTI11C11 Purified Hu WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

TrueMAB

Helicard antibody

Pancreas: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Helicard antibody

Liver: Formalin-Fixed Paraffin-Embedded (FFPE) Rabbit IgG Aff - Purified Hu, Ms P, WB
50 µg / €470.00
  Acris Antibodies GmbH

Helicard antibody

Western blot analysis of extracts from Jurkat cells, using IFIH1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
50 µg / €230.00
  Assay Biotech

Helicard antibody

Western blot analysis of extracts from Jurkat cells, using IFIH1 Antibody. The lane on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms E, WB
0.1 mg / €330.00
  Assay Biotech

CRISPR

Catalog No. Species Donor Vector  

Ifih1 - mouse gene knockout kit via CRISPR

Ifih1 - mouse gene knockout kit via CRISPR Mouse GFP-Puro
1 kit / €1,200.00
  OriGene Technologies, Inc.

Ifih1 - mouse gene knockout kit via CRISPR

Ifih1 - mouse gene knockout kit via CRISPR Mouse mBFP-Neo
1 kit / €1,290.00
  OriGene Technologies, Inc.

Proteins & Growth Factors

Catalog No. Species Pres. Purity Source  

Helicard

  Recombinant protein of human interferon induced with helicase C domain 1 (IFIH1)  
Helicard Human > 80 %
Preparation: Recombint protein was captured through anti-DDK affinity column followed by conventiol chromatography steps.
Purity Detail: > 80% as determined by SDS-PAGE and Coomassie blue staining.
HEK293 cells
20 µg / €680.00
  OriGene Technologies, Inc.

Lysates

Catalog No.    

IFIH1 overexpression lysate

IFIH1 overexpression lysate  
0.1 mg / €480.00
  OriGene Technologies, Inc.

Human Lenti ORF Particles

Catalog No.    

Lenti ORF particles, IFIH1 (mGFP-tagged) - Human interferon induced with helicase C domain 1 (IFIH1), 200 uL, >10^7 TU/mL

Lenti ORF particles, IFIH1 (mGFP-tagged) - Human interferon induced with helicase C domain 1 (IFIH1), 200 uL, >10^7 TU/mL  
200 µl / €1,320.00
  OriGene Technologies, Inc.

Lenti ORF particles, IFIH1 (Myc-DDK tagged) - Human interferon induced with helicase C domain 1 (IFIH1) , 200 uL, >10^7 TU/mL

Lenti ORF particles, IFIH1 (Myc-DDK tagged) - Human interferon induced with helicase C domain 1 (IFIH1) , 200 uL, >10^7 TU/mL  
200 µl / €1,180.00
  OriGene Technologies, Inc.

Mouse Lenti ORF Particles

Catalog No.    

Lenti ORF particles, Ifih1 (GFP-tagged) - Mouse interferon induced with helicase C domain 1 (Ifih1), transcript variant 1, 200 uL, >10^7 TU/mL

Lenti ORF particles, Ifih1 (GFP-tagged) - Mouse interferon induced with helicase C domain 1 (Ifih1), transcript variant 1, 200 uL, >10^7 TU/mL  
200 µl / €1,810.00
  OriGene Technologies, Inc.

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