Hu (Human), Ms (Mouse), Rt (Rat), Mky (Monkey), Bov (Bovine), Can (Canine), Sh (Sheep), Ze (Zebrafish)
Enzyme Immunoassay (E), Western blot / Immunoblot (WB), Immunocytochemistry/Immunofluorescence (ICC/IF)
Background of Hepatic leukemia factor / HLF antibody
This gene encodes a member of the proline and acidic-rich (PAR) protein family, a subset of the bZIP transcription factors. The encoded protein forms homodimers or heterodimers with other PAR family members and binds sequence-specific promoter elements to activate transcription. Chromosomal translocations fusing portions of this gene with the E2A gene cause a subset of childhood B-lineage acute lymphoid leukemias. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Western Blot analysis of HLF expression in transfected 293T cell line by HLF monoclonal antibody (M01), clone 1F12-1C4.Lane 1: HLF transfected lysate(33.2 KDa).Lane 2: Non-transfected lysate.
Western blot analysis of HLF over-expressed 293 cell line, cotransfected with HLF Validated Chimera RNAi ( Cat # H00003131-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with HLF monoclonal antibody (M04), clone M2 (Cat # H00003131-M04 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.
Human HeLa; WB Suggested Anti-HLF Antibody Titration: 0.2-1 ug/ml. ELISA Titer: 1:1562500. Positive Control: Hela cell lysate; HLF antibody - C-terminal region (ARP38108_P050) in Human HeLa cells using Western Blot
HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY HLF (RC206297, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-HLF.
Western blot analysis of extracts (35ug) from 9 different cell lines by using anti-HLF polyclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).