HEXIM1 antibody

Principal name

HEXIM1 antibody

Alternative names for HEXIM1 antibody

HIS1, EDG1, CLP1, MAQ1, Hexamethylene bis-acetamide-inducible protein 1, Estrogen down-regulated gene 1 protein, Cardiac lineage protein 1, Menage a quatre protein 1

SwissProt ID

O94992 (Human), Q0X0C4 (Bovin), Q5M9G1 (Rat), Q8R409 (Mouse)

Gene ID

10614 (HEXIM1)

Available reactivities

Bov (Bovine), Can (Canine), Hu (Human), Ms (Mouse), Rt (Rat), Ze (Zebrafish), Mky (Monkey), Eq (Equine), Por (Porcine), Rb (Rabbit)

Available hosts

Rabbit, Goat, Mouse

Available applications

Western blot / Immunoblot (WB), Enzyme Immunoassay (E), ELISA (detection) (E(detection)), Immunocytochemistry/Immunofluorescence (ICC/IF), Immunoprecipitation (IP), Paraffin Sections (P)

Background of HEXIM1 antibody

Hexamethylene bis-acetamide inducible 1 (HEXIM1) and Hexamethylene bis-acetamide inducible 2 (HEXIM2) comprise a family of proteins which inhibit positive transcription elongation factor b (P-TEFb) through association with 7SK. P-TEFb is composed of a catalytic subunit, Cdk9, and either Cyclin T1 or T2 as a regulatory subunit. This complex regulates eukaryotic gene expression at the level of elongation. The C-terminal domains of HEXIM proteins interact directly with each other. Via these domains, HEXIM1 and HEXIM2 form stable homo- and hetero-oligomers, which may aid in the formation of the 7SK small nuclear ribonucleic acid particle. Despite their similar functions, HEXIM1 and HEXIM2 exhibit distinct expression patterns in various established cell lines and human tissues.

General readings

Schulte A, Czudnochowski N, Barboric M, Schonichen A, Blazek D, Peterlin BM, Geyer M.
Identification of a Cyclin T-Binding Domain in Hexim1 and Biochemical Analysis of Its Binding Competition with HIV-1 Tat.
J Biol Chem. 2005 Jul 1;280(26):24968-77. Epub 2005 Apr 25.
PMID: 15855166

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Primary Antibodies

Catalog No. Host Iso. Clone Pres. React. Applications  

HEXIM1 antibody

TA303065 (0.3µg/ml) staining of 1) HeLa cell lysate and 2) recombinant HEXIM1 (3ng). Detected by chemiluminescence.  Data kindly provided by David Price and Jeff Coopper, University of Iowa, USA. Goat Aff - Purified Hu E, WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HEXIM1 antibody

Western blot analysis of extracts from NIH-3T3 cells, using HES7 antibody.The lane on the right is treated with the synthesized peptide. Rabbit IgG Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 ml / €325.00
  OriGene Technologies, Inc.

HEXIM1 antibody

Predicted cell location: Cytoplasm,Nucleus. Positive control: Human thyroid and liver cancer tissue. Recommended dilution: 1/100-300  The image on the left is immunohistochemistry of paraffin-embedded human thyroid cancer tissue using HEXIM1 antibody at dilution 1/65, on the right is treated with the fusion protein. (Original magnification:x 200) Rabbit IgG Purified Hu, Ms, Rt E, P
0.1 ml / €325.00
  OriGene Technologies, Inc.

HEXIM1 antibody

Western blot analysis of extracts of various cell lines, using HEXIM1 antibody. Rabbit IgG Purified Hu, Ms, Rt
0.1 mg / €325.00
  OriGene Technologies, Inc.

HEXIM1 antibody

WB Suggested Anti-HEXIM1 Antibody Titration: 0.6 ug/ml; ELISA Titer: 1:12500; Positive Control: Jurkat cell lysate Rabbit IgG Purified Bov, Can, Eq, Hu, Ms, Por, Rb, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HEXIM1 antibody

Western blot analysis on NIH-3T3 cell lysate using HEXIM1 Antibody Rabbit IgG Purified Hu, Ms, Rt WB
0.1 mg / €325.00
  OriGene Technologies, Inc.

HEXIM1 (Center) antibody

Immunohistochemistry analysis in formalin fixed and paraffin embedded human placenta tissue reacted with AP52035PU-N followed by peroxidase conjugation of the secondary antibody and DAB staining. Rabbit Ig Aff - Purified Hu, Ms P, WB
0.4 ml / €370.00
  Acris Antibodies GmbH

HEXIM1 antibody

Western Blot analysis of HEXIM1 expression in transfected 293T cell line (H00010614-T01) by HEXIM1 MaxPab polyclonal antibody.

Lane1:HEXIM1 transfected lysate(39.6 KDa).
Lane 2:Non-transfected lysate. Mouse Serum Hu E(detection), WB
50 µl / €370.00
  Abnova Taiwan Corp.

HEXIM1 antibody

Immunofluorescence of purified MaxPab antibody to HEXIM1 on HeLa cell. [antibody concentration 10 ug/ml] Mouse Serum Hu E(detection), ICC/IF, WB
50 µl / €370.00
  Abnova Taiwan Corp.

HEXIM1 antibody

HEXIM1 Mouse IgG2a 4G4 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

HEXIM1 antibody

HEXIM1 Mouse IgG2a 2D12 Purified Hu E
0.1 mg / €370.00
  Abnova Taiwan Corp.

HEXIM1 antibody

HEXIM1 Mouse IgG2b 1H5 Purified Hu E, WB
0.1 mg / €370.00
  Abnova Taiwan Corp.

HEXIM1 antibody

HEXIM1 polyclonal antibody ( Cat # PAB6886 ) ( 0.3 µg/ml ) staining of 1 ) HeLa cell lysate and 2 ) recombinant HEXIM1 ( 3 ng ) . Detected by chemiluminescence. Data kindly provided by David Price and Jeff Coopper, University of Iowa, USA. Goat Hu E, WB
0.1 mg / €400.00
  Abnova Taiwan Corp.

HEXIM1 antibody

Immunofluorescence analysis of HeLa cells, using HEXIM1 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
50 µg / €205.00
  Assay Biotech

HEXIM1 antibody

Immunofluorescence analysis of HeLa cells, using HEXIM1 Antibody. The picture on the right is treated with the synthesized peptide. Rabbit Aff - Purified Hu, Ms, Rt E, ICC/IF, WB
0.1 mg / €310.00
  Assay Biotech

HEXIM1 antibody

Human Jurkat; WB Suggested Anti-HEXIM1 Antibody Titration: 0.6ug/ml. ELISA Titer: 1:12500. Positive Control: Jurkat cell lysate; HEXIM1 antibody - C-terminal region (ARP34420_T100) in Human Jurkat cells using Western Blot Rabbit Aff - Purified Bov, Can, Hu, Ms, Rt, Ze WB
0.1 mg / €310.00
  AVIVA Systems Biology

HEXIM1 antibody

Western blot analysis of HEXIM1 expression in A431 (A); Jurkat (B); NIH3T3 (C); rat heart (D) whole cell lysates. Rabbit Aff - Purified Hu, Mky, Ms, Rt ICC/IF, P, WB
30 µl / €190.00
  Cohesion Biosciences Ltd.

HEXIM1 antibody

Western blot analysis of HEXIM1 expression in A431 (A); Jurkat (B); NIH3T3 (C); rat heart (D) whole cell lysates. Rabbit Aff - Purified Hu, Mky, Ms, Rt ICC/IF, P, WB
0.1 ml / €270.00
  Cohesion Biosciences Ltd.

HEXIM1 antibody

Western blot analysis of HEXIM1 expression in A431 (A); Jurkat (B); NIH3T3 (C); rat heart (D) whole cell lysates. Rabbit Aff - Purified Hu, Mky, Ms, Rt ICC/IF, P, WB
0.2 ml / €440.00
  Cohesion Biosciences Ltd.

HEXIM1 antibody

Western Blot: HEXIM1 Antibody [NB100-55391] - NB100-55391 (0.1ug/ml) staining of Human Brain (Cerebellum) lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence. Goat IgG Aff - Purified Hu E, WB
0.1 mg / €400.00
  Novus Biologicals Inc.

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